A single dose of genetically-attenuated malaria blood-stage parasites protects against two Plasmodium species infections
Genetically-growth-attenuated blood-stage parasites were generated inPlasmodium falciparumby targeted deletion of NT1 (Nucleoside Transporter-1) gene, and Pfnt1(-) parasites only grew after providing the culture with supra-physiological concentrations of purines. Genetically-attenuatedP. yoeliint1(-...
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| Veröffentlicht in: | Vaccine 2023-02, Vol.41 (7), p.1281-1285 |
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| creator | Deveci, Gozde Kamil, Mohd Aly, Ahmed S.I. |
| description | Genetically-growth-attenuated blood-stage parasites were generated inPlasmodium falciparumby targeted deletion of NT1 (Nucleoside Transporter-1) gene, and Pfnt1(-) parasites only grew after providing the culture with supra-physiological concentrations of purines. Genetically-attenuatedP. yoeliint1(-)parasites induced sterile-protection against homologous blood-stage infectious challenge after immunization with single subpatent doses, which remained subpatent even in immune-compromised mice. Here, we showed that immunizations with frozen-stocks of equally-mixedP. bergheiandP. yoelii nt1(-)parasites in single subcutaneous doses, which did not lead to patent blood-stage infection, conferred sterile protection against intravenous infectious blood-stage challenge with wild-type parasites ofP. bergheiANKA andP. yoelii17X-NL strains. This data highlights the possibility that a single subcutaneous sub-patent dose of two species of genetically-growth-attenuated parasites, which can protect humans against twoPlasmodiumspp. infections, could be developed in cultures provided with supra-physiological concentrations of purines, and shipped to endemic areas as frozen-stock doses. |
| doi_str_mv | 10.1016/j.vaccine.2023.01.004 |
| format | Article |
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Genetically-attenuatedP. yoeliint1(-)parasites induced sterile-protection against homologous blood-stage infectious challenge after immunization with single subpatent doses, which remained subpatent even in immune-compromised mice. Here, we showed that immunizations with frozen-stocks of equally-mixedP. bergheiandP. yoelii nt1(-)parasites in single subcutaneous doses, which did not lead to patent blood-stage infection, conferred sterile protection against intravenous infectious blood-stage challenge with wild-type parasites ofP. bergheiANKA andP. yoelii17X-NL strains. This data highlights the possibility that a single subcutaneous sub-patent dose of two species of genetically-growth-attenuated parasites, which can protect humans against twoPlasmodiumspp. infections, could be developed in cultures provided with supra-physiological concentrations of purines, and shipped to endemic areas as frozen-stock doses.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2023.01.004</identifier><identifier>PMID: 36653222</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Animals ; Attenuation ; Blood ; Blood-stage vaccine ; Frozen-stock ; Gene deletion ; genes ; Genotype & phenotype ; Humans ; Immunization ; Infections ; Injections ; intravenous injection ; Malaria ; Malaria Vaccines ; Mice ; Microscopy ; Mixed immunization ; Neurotrophin 1 ; Nucleoside transporter ; nucleosides ; Parasites ; Physiology ; Plasmodium ; Plasmodium berghei ; Plasmodium yoelii ; Purines ; species ; Subcutaneous immunization ; Vaccination ; Vaccines ; Vector-borne diseases</subject><ispartof>Vaccine, 2023-02, Vol.41 (7), p.1281-1285</ispartof><rights>2023</rights><rights>Copyright © 2023. Published by Elsevier Ltd.</rights><rights>Copyright Elsevier Limited Feb 10, 2023</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c374t-60b5f6495d81625e1ad3a85f20d7291e87bdd8017e491e1ec232a286ad2667483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0264410X23000038$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36653222$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deveci, Gozde</creatorcontrib><creatorcontrib>Kamil, Mohd</creatorcontrib><creatorcontrib>Aly, Ahmed S.I.</creatorcontrib><title>A single dose of genetically-attenuated malaria blood-stage parasites protects against two Plasmodium species infections</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Genetically-growth-attenuated blood-stage parasites were generated inPlasmodium falciparumby targeted deletion of NT1 (Nucleoside Transporter-1) gene, and Pfnt1(-) parasites only grew after providing the culture with supra-physiological concentrations of purines. Genetically-attenuatedP. yoeliint1(-)parasites induced sterile-protection against homologous blood-stage infectious challenge after immunization with single subpatent doses, which remained subpatent even in immune-compromised mice. Here, we showed that immunizations with frozen-stocks of equally-mixedP. bergheiandP. yoelii nt1(-)parasites in single subcutaneous doses, which did not lead to patent blood-stage infection, conferred sterile protection against intravenous infectious blood-stage challenge with wild-type parasites ofP. bergheiANKA andP. yoelii17X-NL strains. This data highlights the possibility that a single subcutaneous sub-patent dose of two species of genetically-growth-attenuated parasites, which can protect humans against twoPlasmodiumspp. infections, could be developed in cultures provided with supra-physiological concentrations of purines, and shipped to endemic areas as frozen-stock doses.</description><subject>Animals</subject><subject>Attenuation</subject><subject>Blood</subject><subject>Blood-stage vaccine</subject><subject>Frozen-stock</subject><subject>Gene deletion</subject><subject>genes</subject><subject>Genotype & phenotype</subject><subject>Humans</subject><subject>Immunization</subject><subject>Infections</subject><subject>Injections</subject><subject>intravenous injection</subject><subject>Malaria</subject><subject>Malaria Vaccines</subject><subject>Mice</subject><subject>Microscopy</subject><subject>Mixed immunization</subject><subject>Neurotrophin 1</subject><subject>Nucleoside 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Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deveci, Gozde</au><au>Kamil, Mohd</au><au>Aly, Ahmed S.I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A single dose of genetically-attenuated malaria blood-stage parasites protects against two Plasmodium species infections</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2023-02-10</date><risdate>2023</risdate><volume>41</volume><issue>7</issue><spage>1281</spage><epage>1285</epage><pages>1281-1285</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><abstract>Genetically-growth-attenuated blood-stage parasites were generated inPlasmodium falciparumby targeted deletion of NT1 (Nucleoside Transporter-1) gene, and Pfnt1(-) parasites only grew after providing the culture with supra-physiological concentrations of purines. Genetically-attenuatedP. yoeliint1(-)parasites induced sterile-protection against homologous blood-stage infectious challenge after immunization with single subpatent doses, which remained subpatent even in immune-compromised mice. Here, we showed that immunizations with frozen-stocks of equally-mixedP. bergheiandP. yoelii nt1(-)parasites in single subcutaneous doses, which did not lead to patent blood-stage infection, conferred sterile protection against intravenous infectious blood-stage challenge with wild-type parasites ofP. bergheiANKA andP. yoelii17X-NL strains. This data highlights the possibility that a single subcutaneous sub-patent dose of two species of genetically-growth-attenuated parasites, which can protect humans against twoPlasmodiumspp. infections, could be developed in cultures provided with supra-physiological concentrations of purines, and shipped to endemic areas as frozen-stock doses.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>36653222</pmid><doi>10.1016/j.vaccine.2023.01.004</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Attenuation Blood Blood-stage vaccine Frozen-stock Gene deletion genes Genotype & phenotype Humans Immunization Infections Injections intravenous injection Malaria Malaria Vaccines Mice Microscopy Mixed immunization Neurotrophin 1 Nucleoside transporter nucleosides Parasites Physiology Plasmodium Plasmodium berghei Plasmodium yoelii Purines species Subcutaneous immunization Vaccination Vaccines Vector-borne diseases |
| title | A single dose of genetically-attenuated malaria blood-stage parasites protects against two Plasmodium species infections |
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