Phytogenic blend in the diet of growing Holstein steers: Effects on performance, digestibility, rumen volatile fatty acid profile, and immune and antioxidant responses

The present study determined whether adding the phytogenic blend to the diet of growing steers would improve weight gain, the apparent digestibility of nutrients, volatile fatty acid profiles, and alter immune and antioxidant responses. The experiment included 24 Holstein calves, with an average wei...

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Veröffentlicht in:Animal feed science and technology 2023-03, Vol.297, p.115595, Article 115595
Hauptverfasser: Brunetto, Andrei L.R., Giacomelli, Charles M., Favero, Juscivete F., Bissacotti, Bianca F., Copeti, Priscila M., Morsch, Vera M., de Oliveira, Fernanda de C., Wagner, Roger, Alves, Raissa, Pereira, Wanderson A.B., Vedovatto, Marcelo, Fritzen, Alexandro, Kozloski, Gilberto V., Zotti, Claiton A., Da Silva, Aleksandro S.
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container_start_page 115595
container_title Animal feed science and technology
container_volume 297
creator Brunetto, Andrei L.R.
Giacomelli, Charles M.
Favero, Juscivete F.
Bissacotti, Bianca F.
Copeti, Priscila M.
Morsch, Vera M.
de Oliveira, Fernanda de C.
Wagner, Roger
Alves, Raissa
Pereira, Wanderson A.B.
Vedovatto, Marcelo
Fritzen, Alexandro
Kozloski, Gilberto V.
Zotti, Claiton A.
Da Silva, Aleksandro S.
description The present study determined whether adding the phytogenic blend to the diet of growing steers would improve weight gain, the apparent digestibility of nutrients, volatile fatty acid profiles, and alter immune and antioxidant responses. The experiment included 24 Holstein calves, with an average weight of 149.6 ± 5 kg maintained in a semi-confinement system. The animals were divided into two groups (treated and control). In the concentrate of the treated group, the test product was a phytogenic blend composed of micro-encapsulated cinnamon and oregano essential oils, free turmeric extract, and tannic acid. The animals in the treated group showed more significant weight gain than the control on day 30; there was a trend toward more significant weight gain from 1 to 60 days (P = 0.07). Butyric acid concentrations were higher in the rumen, and there was an interaction between treatment and day (day 30) in the treated group. The ruminal acetic and propionic acid concentrations were higher in animals in the treated group on day 30. The intake of the phytogenic blend increased the apparent digestibility coefficient of neutral detergent fiber but did not affect the apparent digestibility coefficient of dry matter, crude protein, or mineral matter. There were significantly fewer lymphocytes in the treated group on day 30. There was a treatment effect and interaction between treatment and day for cholesterol levels, with lower levels in the treated group than in the control group. Serum glucose concentrations were lower in animals in the treated group. There was an effect of the treatment on the concentration of total proteins and globulins, with the highest levels of these proteins in the animals in the treated group. Protein electrophoresis revealed that IgA and ceruloplasmin levels showed a treatment effect and interaction, with higher levels in the treated group (P 
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The experiment included 24 Holstein calves, with an average weight of 149.6 ± 5 kg maintained in a semi-confinement system. The animals were divided into two groups (treated and control). In the concentrate of the treated group, the test product was a phytogenic blend composed of micro-encapsulated cinnamon and oregano essential oils, free turmeric extract, and tannic acid. The animals in the treated group showed more significant weight gain than the control on day 30; there was a trend toward more significant weight gain from 1 to 60 days (P = 0.07). Butyric acid concentrations were higher in the rumen, and there was an interaction between treatment and day (day 30) in the treated group. The ruminal acetic and propionic acid concentrations were higher in animals in the treated group on day 30. The intake of the phytogenic blend increased the apparent digestibility coefficient of neutral detergent fiber but did not affect the apparent digestibility coefficient of dry matter, crude protein, or mineral matter. There were significantly fewer lymphocytes in the treated group on day 30. There was a treatment effect and interaction between treatment and day for cholesterol levels, with lower levels in the treated group than in the control group. Serum glucose concentrations were lower in animals in the treated group. There was an effect of the treatment on the concentration of total proteins and globulins, with the highest levels of these proteins in the animals in the treated group. Protein electrophoresis revealed that IgA and ceruloplasmin levels showed a treatment effect and interaction, with higher levels in the treated group (P &lt; 0.05). Heavy chain immunoglobulin levels were lower in steers in the treated group at day 90. Thiobarbituric acid reactive substance levels were lower in the treated group, indicating less lipid peroxidation (P &lt; 0.05). We found that the levels of reactive oxygen species were lower in the treatment group at day 30, and glutathione S-transferase activity and protein thiol levels were higher in this group. These findings suggest that adding the phytogenic blend to the animals' diet was critical in the initial phase, as it modulates volatile fatty acids, stimulates immunological biomarkers, and has antioxidant activity. •The addition of the phytogenic blend had a substantial impact in the initial phase on weight gain.•Butyric acid concentrations were higher in the rumen, as well as ruminal acetic and propionic acid.•The intake of the phytogenic blend increased the apparent digestibility coefficient of neutral detergent fiber.•The phytogenic blend increased serum globulins, characterized by the higher of IgA and ceruloplasmin.•The phytogenic blend's minimizes cattle's physiological oxidative stress, as it increase of antioxidants.</description><identifier>ISSN: 0377-8401</identifier><identifier>DOI: 10.1016/j.anifeedsci.2023.115595</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>antioxidant activity ; biomarkers ; blood glucose ; butyric acid ; Cattle ; cholesterol ; cinnamon ; crude protein ; diet ; digestibility ; electrophoresis ; Essential oil ; fatty acid composition ; feeds ; ferroxidase ; glutathione transferase ; Holstein ; immunoglobulins ; lipid peroxidation ; microencapsulation ; neutral detergent fiber ; oregano ; Phytobiotics ; propionic acid ; reactive oxygen species ; Tannic acid ; tannins ; thiobarbituric acid-reactive substances ; thiols ; turmeric ; Turmeric extract ; volatile fatty acids ; weight gain</subject><ispartof>Animal feed science and technology, 2023-03, Vol.297, p.115595, Article 115595</ispartof><rights>2023 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c351t-7d4e298272af1107351f02b1fed115ef79d0beb99a117e7e1024cced12f1d133</citedby><cites>FETCH-LOGICAL-c351t-7d4e298272af1107351f02b1fed115ef79d0beb99a117e7e1024cced12f1d133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0377840123000299$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Brunetto, Andrei L.R.</creatorcontrib><creatorcontrib>Giacomelli, Charles M.</creatorcontrib><creatorcontrib>Favero, Juscivete F.</creatorcontrib><creatorcontrib>Bissacotti, Bianca F.</creatorcontrib><creatorcontrib>Copeti, Priscila M.</creatorcontrib><creatorcontrib>Morsch, Vera M.</creatorcontrib><creatorcontrib>de Oliveira, Fernanda de C.</creatorcontrib><creatorcontrib>Wagner, Roger</creatorcontrib><creatorcontrib>Alves, Raissa</creatorcontrib><creatorcontrib>Pereira, Wanderson A.B.</creatorcontrib><creatorcontrib>Vedovatto, Marcelo</creatorcontrib><creatorcontrib>Fritzen, Alexandro</creatorcontrib><creatorcontrib>Kozloski, Gilberto V.</creatorcontrib><creatorcontrib>Zotti, Claiton A.</creatorcontrib><creatorcontrib>Da Silva, Aleksandro S.</creatorcontrib><title>Phytogenic blend in the diet of growing Holstein steers: Effects on performance, digestibility, rumen volatile fatty acid profile, and immune and antioxidant responses</title><title>Animal feed science and technology</title><description>The present study determined whether adding the phytogenic blend to the diet of growing steers would improve weight gain, the apparent digestibility of nutrients, volatile fatty acid profiles, and alter immune and antioxidant responses. The experiment included 24 Holstein calves, with an average weight of 149.6 ± 5 kg maintained in a semi-confinement system. The animals were divided into two groups (treated and control). In the concentrate of the treated group, the test product was a phytogenic blend composed of micro-encapsulated cinnamon and oregano essential oils, free turmeric extract, and tannic acid. The animals in the treated group showed more significant weight gain than the control on day 30; there was a trend toward more significant weight gain from 1 to 60 days (P = 0.07). Butyric acid concentrations were higher in the rumen, and there was an interaction between treatment and day (day 30) in the treated group. The ruminal acetic and propionic acid concentrations were higher in animals in the treated group on day 30. The intake of the phytogenic blend increased the apparent digestibility coefficient of neutral detergent fiber but did not affect the apparent digestibility coefficient of dry matter, crude protein, or mineral matter. There were significantly fewer lymphocytes in the treated group on day 30. There was a treatment effect and interaction between treatment and day for cholesterol levels, with lower levels in the treated group than in the control group. Serum glucose concentrations were lower in animals in the treated group. There was an effect of the treatment on the concentration of total proteins and globulins, with the highest levels of these proteins in the animals in the treated group. Protein electrophoresis revealed that IgA and ceruloplasmin levels showed a treatment effect and interaction, with higher levels in the treated group (P &lt; 0.05). Heavy chain immunoglobulin levels were lower in steers in the treated group at day 90. Thiobarbituric acid reactive substance levels were lower in the treated group, indicating less lipid peroxidation (P &lt; 0.05). We found that the levels of reactive oxygen species were lower in the treatment group at day 30, and glutathione S-transferase activity and protein thiol levels were higher in this group. These findings suggest that adding the phytogenic blend to the animals' diet was critical in the initial phase, as it modulates volatile fatty acids, stimulates immunological biomarkers, and has antioxidant activity. •The addition of the phytogenic blend had a substantial impact in the initial phase on weight gain.•Butyric acid concentrations were higher in the rumen, as well as ruminal acetic and propionic acid.•The intake of the phytogenic blend increased the apparent digestibility coefficient of neutral detergent fiber.•The phytogenic blend increased serum globulins, characterized by the higher of IgA and ceruloplasmin.•The phytogenic blend's minimizes cattle's physiological oxidative stress, as it increase of antioxidants.</description><subject>antioxidant activity</subject><subject>biomarkers</subject><subject>blood glucose</subject><subject>butyric acid</subject><subject>Cattle</subject><subject>cholesterol</subject><subject>cinnamon</subject><subject>crude protein</subject><subject>diet</subject><subject>digestibility</subject><subject>electrophoresis</subject><subject>Essential oil</subject><subject>fatty acid composition</subject><subject>feeds</subject><subject>ferroxidase</subject><subject>glutathione transferase</subject><subject>Holstein</subject><subject>immunoglobulins</subject><subject>lipid peroxidation</subject><subject>microencapsulation</subject><subject>neutral detergent fiber</subject><subject>oregano</subject><subject>Phytobiotics</subject><subject>propionic acid</subject><subject>reactive oxygen species</subject><subject>Tannic acid</subject><subject>tannins</subject><subject>thiobarbituric acid-reactive substances</subject><subject>thiols</subject><subject>turmeric</subject><subject>Turmeric extract</subject><subject>volatile fatty acids</subject><subject>weight gain</subject><issn>0377-8401</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNqFUbFuGzEMvaEBmib9B44dbEfU2T1ftzRImgIB2iG7oJMoh8ad5EpyWn9Rf7N0XCBjFpIg36PE95oGUC1Q4eer7cJGDkS-OF5opdsF4mrVr94156rtuvl6qfB986GUrVKo160-b_7-fDrUtKHIDoaRogeOUJ8IPFOFFGCT02-OG7hPY6kkQ4mUyxe4DYFcLZAi7CiHlCcbHc2EuKFSeeCR62EGeT9RhOc02sojQbC1HsA69rDLKUhrBvb46jTtI72UNlZOf9hLhkxll2KhctmcBTsW-vg_XzSPd7ePN_fzhx_fvt9cP8xdu8I67_ySdL_WnbYBUXXSDEoPGMiLFBS63quBhr63iB11hEovnZOhDuixbS-aT6e18rlfe7nDTFwcjaONlPbFiGhLERY1CnR9grqcSskUzC7zZPPBoDJHO8zWvNphjnaYkx1C_XqiklzyzJSNIEjU85xFU-MTv73kHwI3np8</recordid><startdate>202303</startdate><enddate>202303</enddate><creator>Brunetto, Andrei L.R.</creator><creator>Giacomelli, Charles M.</creator><creator>Favero, Juscivete F.</creator><creator>Bissacotti, Bianca F.</creator><creator>Copeti, Priscila M.</creator><creator>Morsch, Vera M.</creator><creator>de Oliveira, Fernanda de C.</creator><creator>Wagner, Roger</creator><creator>Alves, Raissa</creator><creator>Pereira, Wanderson A.B.</creator><creator>Vedovatto, Marcelo</creator><creator>Fritzen, Alexandro</creator><creator>Kozloski, Gilberto V.</creator><creator>Zotti, Claiton A.</creator><creator>Da Silva, Aleksandro S.</creator><general>Elsevier B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>202303</creationdate><title>Phytogenic blend in the diet of growing Holstein steers: Effects on performance, digestibility, rumen volatile fatty acid profile, and immune and antioxidant responses</title><author>Brunetto, Andrei L.R. ; Giacomelli, Charles M. ; Favero, Juscivete F. ; Bissacotti, Bianca F. ; Copeti, Priscila M. ; Morsch, Vera M. ; de Oliveira, Fernanda de C. ; Wagner, Roger ; Alves, Raissa ; Pereira, Wanderson A.B. ; Vedovatto, Marcelo ; Fritzen, Alexandro ; Kozloski, Gilberto V. ; Zotti, Claiton A. ; Da Silva, Aleksandro S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c351t-7d4e298272af1107351f02b1fed115ef79d0beb99a117e7e1024cced12f1d133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>antioxidant activity</topic><topic>biomarkers</topic><topic>blood glucose</topic><topic>butyric acid</topic><topic>Cattle</topic><topic>cholesterol</topic><topic>cinnamon</topic><topic>crude protein</topic><topic>diet</topic><topic>digestibility</topic><topic>electrophoresis</topic><topic>Essential oil</topic><topic>fatty acid composition</topic><topic>feeds</topic><topic>ferroxidase</topic><topic>glutathione transferase</topic><topic>Holstein</topic><topic>immunoglobulins</topic><topic>lipid peroxidation</topic><topic>microencapsulation</topic><topic>neutral detergent fiber</topic><topic>oregano</topic><topic>Phytobiotics</topic><topic>propionic acid</topic><topic>reactive oxygen species</topic><topic>Tannic acid</topic><topic>tannins</topic><topic>thiobarbituric acid-reactive substances</topic><topic>thiols</topic><topic>turmeric</topic><topic>Turmeric extract</topic><topic>volatile fatty acids</topic><topic>weight gain</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brunetto, Andrei L.R.</creatorcontrib><creatorcontrib>Giacomelli, Charles M.</creatorcontrib><creatorcontrib>Favero, Juscivete F.</creatorcontrib><creatorcontrib>Bissacotti, Bianca F.</creatorcontrib><creatorcontrib>Copeti, Priscila M.</creatorcontrib><creatorcontrib>Morsch, Vera M.</creatorcontrib><creatorcontrib>de Oliveira, Fernanda de C.</creatorcontrib><creatorcontrib>Wagner, Roger</creatorcontrib><creatorcontrib>Alves, Raissa</creatorcontrib><creatorcontrib>Pereira, Wanderson A.B.</creatorcontrib><creatorcontrib>Vedovatto, Marcelo</creatorcontrib><creatorcontrib>Fritzen, Alexandro</creatorcontrib><creatorcontrib>Kozloski, Gilberto V.</creatorcontrib><creatorcontrib>Zotti, Claiton A.</creatorcontrib><creatorcontrib>Da Silva, Aleksandro S.</creatorcontrib><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Animal feed science and technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brunetto, Andrei L.R.</au><au>Giacomelli, Charles M.</au><au>Favero, Juscivete F.</au><au>Bissacotti, Bianca F.</au><au>Copeti, Priscila M.</au><au>Morsch, Vera M.</au><au>de Oliveira, Fernanda de C.</au><au>Wagner, Roger</au><au>Alves, Raissa</au><au>Pereira, Wanderson A.B.</au><au>Vedovatto, Marcelo</au><au>Fritzen, Alexandro</au><au>Kozloski, Gilberto V.</au><au>Zotti, Claiton A.</au><au>Da Silva, Aleksandro S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phytogenic blend in the diet of growing Holstein steers: Effects on performance, digestibility, rumen volatile fatty acid profile, and immune and antioxidant responses</atitle><jtitle>Animal feed science and technology</jtitle><date>2023-03</date><risdate>2023</risdate><volume>297</volume><spage>115595</spage><pages>115595-</pages><artnum>115595</artnum><issn>0377-8401</issn><abstract>The present study determined whether adding the phytogenic blend to the diet of growing steers would improve weight gain, the apparent digestibility of nutrients, volatile fatty acid profiles, and alter immune and antioxidant responses. The experiment included 24 Holstein calves, with an average weight of 149.6 ± 5 kg maintained in a semi-confinement system. The animals were divided into two groups (treated and control). In the concentrate of the treated group, the test product was a phytogenic blend composed of micro-encapsulated cinnamon and oregano essential oils, free turmeric extract, and tannic acid. The animals in the treated group showed more significant weight gain than the control on day 30; there was a trend toward more significant weight gain from 1 to 60 days (P = 0.07). Butyric acid concentrations were higher in the rumen, and there was an interaction between treatment and day (day 30) in the treated group. The ruminal acetic and propionic acid concentrations were higher in animals in the treated group on day 30. The intake of the phytogenic blend increased the apparent digestibility coefficient of neutral detergent fiber but did not affect the apparent digestibility coefficient of dry matter, crude protein, or mineral matter. There were significantly fewer lymphocytes in the treated group on day 30. There was a treatment effect and interaction between treatment and day for cholesterol levels, with lower levels in the treated group than in the control group. Serum glucose concentrations were lower in animals in the treated group. There was an effect of the treatment on the concentration of total proteins and globulins, with the highest levels of these proteins in the animals in the treated group. Protein electrophoresis revealed that IgA and ceruloplasmin levels showed a treatment effect and interaction, with higher levels in the treated group (P &lt; 0.05). Heavy chain immunoglobulin levels were lower in steers in the treated group at day 90. Thiobarbituric acid reactive substance levels were lower in the treated group, indicating less lipid peroxidation (P &lt; 0.05). We found that the levels of reactive oxygen species were lower in the treatment group at day 30, and glutathione S-transferase activity and protein thiol levels were higher in this group. These findings suggest that adding the phytogenic blend to the animals' diet was critical in the initial phase, as it modulates volatile fatty acids, stimulates immunological biomarkers, and has antioxidant activity. •The addition of the phytogenic blend had a substantial impact in the initial phase on weight gain.•Butyric acid concentrations were higher in the rumen, as well as ruminal acetic and propionic acid.•The intake of the phytogenic blend increased the apparent digestibility coefficient of neutral detergent fiber.•The phytogenic blend increased serum globulins, characterized by the higher of IgA and ceruloplasmin.•The phytogenic blend's minimizes cattle's physiological oxidative stress, as it increase of antioxidants.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.anifeedsci.2023.115595</doi></addata></record>
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source Elsevier ScienceDirect Journals
subjects antioxidant activity
biomarkers
blood glucose
butyric acid
Cattle
cholesterol
cinnamon
crude protein
diet
digestibility
electrophoresis
Essential oil
fatty acid composition
feeds
ferroxidase
glutathione transferase
Holstein
immunoglobulins
lipid peroxidation
microencapsulation
neutral detergent fiber
oregano
Phytobiotics
propionic acid
reactive oxygen species
Tannic acid
tannins
thiobarbituric acid-reactive substances
thiols
turmeric
Turmeric extract
volatile fatty acids
weight gain
title Phytogenic blend in the diet of growing Holstein steers: Effects on performance, digestibility, rumen volatile fatty acid profile, and immune and antioxidant responses
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