In vitro and in silico assessment of anti-quorum sensing activity of Naproxen against Pseudomonas aeruginosa
Serious infections caused by Pseudomonas aeruginosa are usually related to quorum sensing (QS)-dependent virulence factors. Hence, QS inhibition is a promising approach to overcoming P. aeruginosa infections. This study aimed to investigate the effect of naproxen on biofilm formation and QS-related...
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| Veröffentlicht in: | World journal of microbiology & biotechnology 2023-09, Vol.39 (9), p.244-244, Article 244 |
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| creator | Esnaashari, Fatemeh Rostamnejad, Dorna Zahmatkesh, Hossein Zamani, Hojjatolah |
| description | Serious infections caused by
Pseudomonas aeruginosa
are usually related to quorum sensing (QS)-dependent virulence factors. Hence, QS inhibition is a promising approach to overcoming
P. aeruginosa
infections. This study aimed to investigate the effect of naproxen on biofilm formation and QS-related virulence traits of
P. aeruginosa
. Furthermore, the anti-QS potential of naproxen was evaluated using real-time PCR and molecular docking analysis. Our findings supported the anti-QS activity of naproxen, as evidenced by down-regulation of the
lasI
and
rhlI
genes expression as well as the attenuation of bacterial protease, hemolysin, pyocyanin, biofilm, and motility. Additionally, the high binding affinity of naproxen with QS regulatory proteins was determined in the molecular docking simulation. Altogether, these findings suggest that naproxen has a promising potential in inhibiting QS-associated traits of
P. aeruginosa
. |
| doi_str_mv | 10.1007/s11274-023-03690-5 |
| format | Article |
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Pseudomonas aeruginosa
are usually related to quorum sensing (QS)-dependent virulence factors. Hence, QS inhibition is a promising approach to overcoming
P. aeruginosa
infections. This study aimed to investigate the effect of naproxen on biofilm formation and QS-related virulence traits of
P. aeruginosa
. Furthermore, the anti-QS potential of naproxen was evaluated using real-time PCR and molecular docking analysis. Our findings supported the anti-QS activity of naproxen, as evidenced by down-regulation of the
lasI
and
rhlI
genes expression as well as the attenuation of bacterial protease, hemolysin, pyocyanin, biofilm, and motility. Additionally, the high binding affinity of naproxen with QS regulatory proteins was determined in the molecular docking simulation. Altogether, these findings suggest that naproxen has a promising potential in inhibiting QS-associated traits of
P. aeruginosa
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Pseudomonas aeruginosa
are usually related to quorum sensing (QS)-dependent virulence factors. Hence, QS inhibition is a promising approach to overcoming
P. aeruginosa
infections. This study aimed to investigate the effect of naproxen on biofilm formation and QS-related virulence traits of
P. aeruginosa
. Furthermore, the anti-QS potential of naproxen was evaluated using real-time PCR and molecular docking analysis. Our findings supported the anti-QS activity of naproxen, as evidenced by down-regulation of the
lasI
and
rhlI
genes expression as well as the attenuation of bacterial protease, hemolysin, pyocyanin, biofilm, and motility. Additionally, the high binding affinity of naproxen with QS regulatory proteins was determined in the molecular docking simulation. Altogether, these findings suggest that naproxen has a promising potential in inhibiting QS-associated traits of
P. aeruginosa
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Fatemeh</au><au>Rostamnejad, Dorna</au><au>Zahmatkesh, Hossein</au><au>Zamani, Hojjatolah</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro and in silico assessment of anti-quorum sensing activity of Naproxen against Pseudomonas aeruginosa</atitle><jtitle>World journal of microbiology & biotechnology</jtitle><stitle>World J Microbiol Biotechnol</stitle><addtitle>World J Microbiol Biotechnol</addtitle><date>2023-09-01</date><risdate>2023</risdate><volume>39</volume><issue>9</issue><spage>244</spage><epage>244</epage><pages>244-244</pages><artnum>244</artnum><issn>0959-3993</issn><eissn>1573-0972</eissn><abstract>Serious infections caused by
Pseudomonas aeruginosa
are usually related to quorum sensing (QS)-dependent virulence factors. Hence, QS inhibition is a promising approach to overcoming
P. aeruginosa
infections. This study aimed to investigate the effect of naproxen on biofilm formation and QS-related virulence traits of
P. aeruginosa
. Furthermore, the anti-QS potential of naproxen was evaluated using real-time PCR and molecular docking analysis. Our findings supported the anti-QS activity of naproxen, as evidenced by down-regulation of the
lasI
and
rhlI
genes expression as well as the attenuation of bacterial protease, hemolysin, pyocyanin, biofilm, and motility. Additionally, the high binding affinity of naproxen with QS regulatory proteins was determined in the molecular docking simulation. Altogether, these findings suggest that naproxen has a promising potential in inhibiting QS-associated traits of
P. aeruginosa
.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>37407806</pmid><doi>10.1007/s11274-023-03690-5</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-1622-4530</orcidid><orcidid>https://orcid.org/0000-0003-2765-7718</orcidid></addata></record> |
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| identifier | ISSN: 0959-3993 |
| ispartof | World journal of microbiology & biotechnology, 2023-09, Vol.39 (9), p.244-244, Article 244 |
| issn | 0959-3993 1573-0972 |
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| subjects | Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Antibiotics Applied Microbiology Bacterial infections Bacterial Proteins - metabolism Biochemistry Biofilms Biomedical and Life Sciences Biotechnology Cystic fibrosis Drug resistance Environmental Engineering/Biotechnology Gene expression Infections Life Sciences Microbiology Molecular docking Molecular Docking Simulation Motility Naproxen Naproxen - pharmacology Pathogenesis Pseudomonas aeruginosa Pyocyanin Quorum Sensing Regulatory proteins Virulence Virulence factors Virulence Factors - genetics Virulence Factors - metabolism |
| title | In vitro and in silico assessment of anti-quorum sensing activity of Naproxen against Pseudomonas aeruginosa |
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