Molecular characterization of a novel ambiguivirus isolated from the phytopathogenic fungus Setosphaeria turcica
In this study, a novel single-stranded RNA virus was isolated from the plant-pathogenic fungus Setosphaeria turcica strain TG2, and the virus was named "Setosphaeria turcica ambiguivirus 2" (StAV2). The complete nucleotide sequence of the StAV2 genome was determined using RT-PCR and RLM-RA...
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| Veröffentlicht in: | Archives of virology 2023-08, Vol.168 (8), p.199-199, Article 199 |
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| creator | Jia, Jichun Chen, Xu Wang, Xue Liu, Xu Zhang, Nuo Zhang, Baojun Chang, Yindong Mu, Fan |
| description | In this study, a novel single-stranded RNA virus was isolated from the plant-pathogenic fungus
Setosphaeria turcica
strain TG2, and the virus was named "Setosphaeria turcica ambiguivirus 2" (StAV2). The complete nucleotide sequence of the StAV2 genome was determined using RT-PCR and RLM-RACE. The StAV2 genome comprises 3,000 nucleotides with a G+C content of 57.77%. StAV2 contains two in-frame open reading frames (ORFs) with the potential to produce an ORF1-ORF2 fusion protein via a stop codon readthrough mechanism. ORF1 encodes a hypothetical protein (HP) of unknown function. The ORF2-encoded protein shows a high degree of sequence similarity to the RNA-dependent RNA polymerases (RdRps) of ambiguiviruses. BLASTp searches showed that the StAV2 HP and RdRp share the highest amino acid sequence identity (46.38% and 69.23%, respectively) with the corresponding proteins of a virus identified as "
Riboviria
sp." isolated from a soil sample. Multiple sequence alignments and phylogenetic analysis based on the amino acid sequences of the RdRp revealed that StAV2 is a new member of the proposed family "
Ambiguiviridae
". |
| doi_str_mv | 10.1007/s00705-023-05829-z |
| format | Article |
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Setosphaeria turcica
strain TG2, and the virus was named "Setosphaeria turcica ambiguivirus 2" (StAV2). The complete nucleotide sequence of the StAV2 genome was determined using RT-PCR and RLM-RACE. The StAV2 genome comprises 3,000 nucleotides with a G+C content of 57.77%. StAV2 contains two in-frame open reading frames (ORFs) with the potential to produce an ORF1-ORF2 fusion protein via a stop codon readthrough mechanism. ORF1 encodes a hypothetical protein (HP) of unknown function. The ORF2-encoded protein shows a high degree of sequence similarity to the RNA-dependent RNA polymerases (RdRps) of ambiguiviruses. BLASTp searches showed that the StAV2 HP and RdRp share the highest amino acid sequence identity (46.38% and 69.23%, respectively) with the corresponding proteins of a virus identified as "
Riboviria
sp." isolated from a soil sample. Multiple sequence alignments and phylogenetic analysis based on the amino acid sequences of the RdRp revealed that StAV2 is a new member of the proposed family "
Ambiguiviridae
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Setosphaeria turcica
strain TG2, and the virus was named "Setosphaeria turcica ambiguivirus 2" (StAV2). The complete nucleotide sequence of the StAV2 genome was determined using RT-PCR and RLM-RACE. The StAV2 genome comprises 3,000 nucleotides with a G+C content of 57.77%. StAV2 contains two in-frame open reading frames (ORFs) with the potential to produce an ORF1-ORF2 fusion protein via a stop codon readthrough mechanism. ORF1 encodes a hypothetical protein (HP) of unknown function. The ORF2-encoded protein shows a high degree of sequence similarity to the RNA-dependent RNA polymerases (RdRps) of ambiguiviruses. BLASTp searches showed that the StAV2 HP and RdRp share the highest amino acid sequence identity (46.38% and 69.23%, respectively) with the corresponding proteins of a virus identified as "
Riboviria
sp." isolated from a soil sample. Multiple sequence alignments and phylogenetic analysis based on the amino acid sequences of the RdRp revealed that StAV2 is a new member of the proposed family "
Ambiguiviridae
".</description><subject>Ambiguiviridae</subject><subject>Amino acid sequence</subject><subject>Annotated Sequence Record</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Fusion protein</subject><subject>Genomes</subject><subject>Infectious Diseases</subject><subject>Medical Microbiology</subject><subject>Nucleotide sequence</subject><subject>Open reading frames</subject><subject>Phylogeny</subject><subject>Phytopathogenic fungi</subject><subject>Proteins</subject><subject>RNA viruses</subject><subject>Setosphaeria turcica</subject><subject>Stop codon</subject><subject>Virology</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1vFCEYgInR2G31D3gwJF68TH35WBaOTdPaJjUe1DNhgdmhmRmmfDTp_nppt9rEgxdI4HkfSB6EPhA4JQCbL7ktsO6Asg7Wkqpu_wqtCGe0kxslX6MVMOCdFCCP0HHOtwDtgK3foiO24QBCsBVavsXR2zqahO1gkrHFp7A3JcQZxx4bPMd7P2IzbcOuhvuQasYhx9EU73Cf4oTL4PEyPJS4mDLEnZ-DxX2ddw384UvMy2Ca0uBSkw3WvENvejNm__55P0G_Li9-nl91N9-_Xp-f3XSWUVE6S5gwRlHh3NoptfGut1YYKqijW-qIsJIBJUL0cguccyGIdIoyqZRiVnF2gj4fvEuKd9XnoqeQrR9HM_tYs6aSNQGnQBr66R_0NtY0t989UlQwJjltFD1QNsWck-_1ksJk0oMmoB976EMP3Xropx5634Y-PqvrdvLu78ifAA1gByC3q3nn08vb_9H-Bp4Al6U</recordid><startdate>20230801</startdate><enddate>20230801</enddate><creator>Jia, Jichun</creator><creator>Chen, Xu</creator><creator>Wang, Xue</creator><creator>Liu, Xu</creator><creator>Zhang, Nuo</creator><creator>Zhang, Baojun</creator><creator>Chang, Yindong</creator><creator>Mu, Fan</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0009-0003-8760-8181</orcidid><orcidid>https://orcid.org/0009-0000-2797-6685</orcidid></search><sort><creationdate>20230801</creationdate><title>Molecular characterization of a novel ambiguivirus isolated from the phytopathogenic fungus Setosphaeria turcica</title><author>Jia, Jichun ; 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Setosphaeria turcica
strain TG2, and the virus was named "Setosphaeria turcica ambiguivirus 2" (StAV2). The complete nucleotide sequence of the StAV2 genome was determined using RT-PCR and RLM-RACE. The StAV2 genome comprises 3,000 nucleotides with a G+C content of 57.77%. StAV2 contains two in-frame open reading frames (ORFs) with the potential to produce an ORF1-ORF2 fusion protein via a stop codon readthrough mechanism. ORF1 encodes a hypothetical protein (HP) of unknown function. The ORF2-encoded protein shows a high degree of sequence similarity to the RNA-dependent RNA polymerases (RdRps) of ambiguiviruses. BLASTp searches showed that the StAV2 HP and RdRp share the highest amino acid sequence identity (46.38% and 69.23%, respectively) with the corresponding proteins of a virus identified as "
Riboviria
sp." isolated from a soil sample. Multiple sequence alignments and phylogenetic analysis based on the amino acid sequences of the RdRp revealed that StAV2 is a new member of the proposed family "
Ambiguiviridae
".</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>37400663</pmid><doi>10.1007/s00705-023-05829-z</doi><tpages>1</tpages><orcidid>https://orcid.org/0009-0003-8760-8181</orcidid><orcidid>https://orcid.org/0009-0000-2797-6685</orcidid></addata></record> |
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| ispartof | Archives of virology, 2023-08, Vol.168 (8), p.199-199, Article 199 |
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| subjects | Ambiguiviridae Amino acid sequence Annotated Sequence Record Biomedical and Life Sciences Biomedicine Fusion protein Genomes Infectious Diseases Medical Microbiology Nucleotide sequence Open reading frames Phylogeny Phytopathogenic fungi Proteins RNA viruses Setosphaeria turcica Stop codon Virology |
| title | Molecular characterization of a novel ambiguivirus isolated from the phytopathogenic fungus Setosphaeria turcica |
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