Antineoplastic Effects of Mucuna pruriens Against Human Colorectal Adenocarcinoma
Mucuna pruriens (MP) which is commonly known as “Velvet Bean” is an underutilized legume traditionally used to treat Parkinson’s disease and male fertility issues. Extracts of MP have also been identified for their antidiabetic, antioxidant, and antineoplastic effects. Commonly, the antioxidant and...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2024-03, Vol.196 (3), p.1350-1364 |
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| creator | Seetharamaiah, Sagar Muddappa, Vidya Shimoga Krishnaswamy, Manjunatha Bukkambudhi Vasappa, Rashmi Kanugodu |
| description | Mucuna pruriens
(MP) which is commonly known as “Velvet Bean” is an underutilized legume traditionally used to treat Parkinson’s disease and male fertility issues. Extracts of MP have also been identified for their antidiabetic, antioxidant, and antineoplastic effects. Commonly, the antioxidant and anticancer properties of a drug are linked together as antioxidants scavenge free radicals and prevent the cellular DNA damage which could result in cancer development. In this investigation, comparative assessment of the anticancer and antioxidant potentials of methanolic seed extracts from two common varieties of MP,
Mucuna pruriens var. pruriens
(MPP) and
Mucuna pruriens var. utilis
(MPU) against human colorectal cancer adenocarcinoma cells COLO-205, was carried out. The highest antioxidant potential was recorded with MPP with an IC
50
of 45.71 μg/ml. The in vitro antiproliferative effects of MPP and MPU on COLO-205 showed an IC
50
of 131.1 μg/ml and 246.9 μg/ml respectively. Our results revealed intervention of the MPP and MPU extracts in growth kinetics of the COLO-205 cells in concomitance with apoptosis induction up to 8.73- and 5.58-folds respectively. The AO/EtBr dual staining and the flow cytometry results also confirmed the better apoptotic efficacy of MPP over MPU. MPP at a concentration of 160 μg/ml exhibited highest apoptosis and cell cycle arrest. Furthermore, effect of the seed extracts on p53 expression was investigated by quantitative RT-PCR and a maximum upregulation of 1.12-fold was recorded with MPP. |
| doi_str_mv | 10.1007/s12010-023-04598-4 |
| format | Article |
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(MP) which is commonly known as “Velvet Bean” is an underutilized legume traditionally used to treat Parkinson’s disease and male fertility issues. Extracts of MP have also been identified for their antidiabetic, antioxidant, and antineoplastic effects. Commonly, the antioxidant and anticancer properties of a drug are linked together as antioxidants scavenge free radicals and prevent the cellular DNA damage which could result in cancer development. In this investigation, comparative assessment of the anticancer and antioxidant potentials of methanolic seed extracts from two common varieties of MP,
Mucuna pruriens var. pruriens
(MPP) and
Mucuna pruriens var. utilis
(MPU) against human colorectal cancer adenocarcinoma cells COLO-205, was carried out. The highest antioxidant potential was recorded with MPP with an IC
50
of 45.71 μg/ml. The in vitro antiproliferative effects of MPP and MPU on COLO-205 showed an IC
50
of 131.1 μg/ml and 246.9 μg/ml respectively. Our results revealed intervention of the MPP and MPU extracts in growth kinetics of the COLO-205 cells in concomitance with apoptosis induction up to 8.73- and 5.58-folds respectively. The AO/EtBr dual staining and the flow cytometry results also confirmed the better apoptotic efficacy of MPP over MPU. MPP at a concentration of 160 μg/ml exhibited highest apoptosis and cell cycle arrest. Furthermore, effect of the seed extracts on p53 expression was investigated by quantitative RT-PCR and a maximum upregulation of 1.12-fold was recorded with MPP.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-023-04598-4</identifier><identifier>PMID: 37395947</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adenocarcinoma ; Adenocarcinoma - drug therapy ; Anticancer properties ; Antineoplastic Agents - pharmacology ; Antioxidants ; Antioxidants - pharmacology ; Apoptosis ; Biochemistry ; Biotechnology ; Cancer ; Cell cycle ; Chemistry ; Chemistry and Materials Science ; Colorectal cancer ; Colorectal carcinoma ; Colorectal Neoplasms - drug therapy ; Damage prevention ; Diabetes mellitus ; DNA damage ; Fertility ; Flow cytometry ; Free radicals ; Growth kinetics ; Humans ; Legumes ; Male ; Movement disorders ; Mucuna ; Mucuna pruriens ; Neurodegenerative diseases ; Original Article ; Parkinson's disease ; Plant Extracts - pharmacology ; Seeds</subject><ispartof>Applied biochemistry and biotechnology, 2024-03, Vol.196 (3), p.1350-1364</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-e2e10c72af3c8763a96cde5bb7de17641613ed7a745a44e046fc24f2d0cae4f73</citedby><cites>FETCH-LOGICAL-c375t-e2e10c72af3c8763a96cde5bb7de17641613ed7a745a44e046fc24f2d0cae4f73</cites><orcidid>0000-0001-6994-5182 ; 0000-0003-1319-7466 ; 0000-0002-6545-1983</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-023-04598-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-023-04598-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37395947$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Seetharamaiah, Sagar</creatorcontrib><creatorcontrib>Muddappa, Vidya Shimoga</creatorcontrib><creatorcontrib>Krishnaswamy, Manjunatha Bukkambudhi</creatorcontrib><creatorcontrib>Vasappa, Rashmi Kanugodu</creatorcontrib><title>Antineoplastic Effects of Mucuna pruriens Against Human Colorectal Adenocarcinoma</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>Mucuna pruriens
(MP) which is commonly known as “Velvet Bean” is an underutilized legume traditionally used to treat Parkinson’s disease and male fertility issues. Extracts of MP have also been identified for their antidiabetic, antioxidant, and antineoplastic effects. Commonly, the antioxidant and anticancer properties of a drug are linked together as antioxidants scavenge free radicals and prevent the cellular DNA damage which could result in cancer development. In this investigation, comparative assessment of the anticancer and antioxidant potentials of methanolic seed extracts from two common varieties of MP,
Mucuna pruriens var. pruriens
(MPP) and
Mucuna pruriens var. utilis
(MPU) against human colorectal cancer adenocarcinoma cells COLO-205, was carried out. The highest antioxidant potential was recorded with MPP with an IC
50
of 45.71 μg/ml. The in vitro antiproliferative effects of MPP and MPU on COLO-205 showed an IC
50
of 131.1 μg/ml and 246.9 μg/ml respectively. Our results revealed intervention of the MPP and MPU extracts in growth kinetics of the COLO-205 cells in concomitance with apoptosis induction up to 8.73- and 5.58-folds respectively. The AO/EtBr dual staining and the flow cytometry results also confirmed the better apoptotic efficacy of MPP over MPU. MPP at a concentration of 160 μg/ml exhibited highest apoptosis and cell cycle arrest. Furthermore, effect of the seed extracts on p53 expression was investigated by quantitative RT-PCR and a maximum upregulation of 1.12-fold was recorded with MPP.</description><subject>Adenocarcinoma</subject><subject>Adenocarcinoma - drug therapy</subject><subject>Anticancer properties</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>Apoptosis</subject><subject>Biochemistry</subject><subject>Biotechnology</subject><subject>Cancer</subject><subject>Cell cycle</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Colorectal cancer</subject><subject>Colorectal carcinoma</subject><subject>Colorectal Neoplasms - drug therapy</subject><subject>Damage prevention</subject><subject>Diabetes mellitus</subject><subject>DNA damage</subject><subject>Fertility</subject><subject>Flow cytometry</subject><subject>Free radicals</subject><subject>Growth kinetics</subject><subject>Humans</subject><subject>Legumes</subject><subject>Male</subject><subject>Movement disorders</subject><subject>Mucuna</subject><subject>Mucuna pruriens</subject><subject>Neurodegenerative diseases</subject><subject>Original Article</subject><subject>Parkinson's disease</subject><subject>Plant Extracts - pharmacology</subject><subject>Seeds</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKxDAUhoMozjj6Ai6k4MZNNdemWQ6DNxgRQdchk55IpU3GpF349kbrBVy4OpDz_X-SD6Fjgs8JxvIiEYoJLjFlJeZC1SXfQXMihMpHiuyiOaaSlZTWaoYOUnrBmNBayH00Y5Ipobico4elH1oPYduZNLS2uHQO7JCK4Iq70Y7eFNs4xhZ8KpbPpvVpKG7G3vhiFboQM2q6YtmAD9ZE2_rQm0O050yX4OhrLtDT1eXj6qZc31_frpbr0jIphhIoEGwlNY7ZWlbMqMo2IDYb2QCRFScVYdBII7kwnAPmlbOUO9pga4A7yRbobOrdxvA6Qhp03yYLXWfyd8akac1ozQnlIqOnf9CXMEafX6epyhDntaKZohNlY0gpgtPb2PYmvmmC9YdwPQnXWbj-FK55Dp18VY-bHpqfyLfhDLAJSHnlnyH-3v1P7TuTpIth</recordid><startdate>20240301</startdate><enddate>20240301</enddate><creator>Seetharamaiah, Sagar</creator><creator>Muddappa, Vidya Shimoga</creator><creator>Krishnaswamy, Manjunatha Bukkambudhi</creator><creator>Vasappa, Rashmi Kanugodu</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6994-5182</orcidid><orcidid>https://orcid.org/0000-0003-1319-7466</orcidid><orcidid>https://orcid.org/0000-0002-6545-1983</orcidid></search><sort><creationdate>20240301</creationdate><title>Antineoplastic Effects of Mucuna pruriens Against Human Colorectal Adenocarcinoma</title><author>Seetharamaiah, Sagar ; Muddappa, Vidya Shimoga ; Krishnaswamy, Manjunatha Bukkambudhi ; Vasappa, Rashmi Kanugodu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-e2e10c72af3c8763a96cde5bb7de17641613ed7a745a44e046fc24f2d0cae4f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Adenocarcinoma</topic><topic>Adenocarcinoma - drug therapy</topic><topic>Anticancer properties</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Antioxidants</topic><topic>Antioxidants - pharmacology</topic><topic>Apoptosis</topic><topic>Biochemistry</topic><topic>Biotechnology</topic><topic>Cancer</topic><topic>Cell cycle</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Colorectal cancer</topic><topic>Colorectal carcinoma</topic><topic>Colorectal Neoplasms - drug therapy</topic><topic>Damage prevention</topic><topic>Diabetes mellitus</topic><topic>DNA damage</topic><topic>Fertility</topic><topic>Flow cytometry</topic><topic>Free radicals</topic><topic>Growth kinetics</topic><topic>Humans</topic><topic>Legumes</topic><topic>Male</topic><topic>Movement disorders</topic><topic>Mucuna</topic><topic>Mucuna pruriens</topic><topic>Neurodegenerative diseases</topic><topic>Original Article</topic><topic>Parkinson's disease</topic><topic>Plant Extracts - pharmacology</topic><topic>Seeds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seetharamaiah, Sagar</creatorcontrib><creatorcontrib>Muddappa, Vidya Shimoga</creatorcontrib><creatorcontrib>Krishnaswamy, Manjunatha Bukkambudhi</creatorcontrib><creatorcontrib>Vasappa, Rashmi Kanugodu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seetharamaiah, Sagar</au><au>Muddappa, Vidya Shimoga</au><au>Krishnaswamy, Manjunatha Bukkambudhi</au><au>Vasappa, Rashmi Kanugodu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antineoplastic Effects of Mucuna pruriens Against Human Colorectal Adenocarcinoma</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2024-03-01</date><risdate>2024</risdate><volume>196</volume><issue>3</issue><spage>1350</spage><epage>1364</epage><pages>1350-1364</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><abstract>Mucuna pruriens
(MP) which is commonly known as “Velvet Bean” is an underutilized legume traditionally used to treat Parkinson’s disease and male fertility issues. Extracts of MP have also been identified for their antidiabetic, antioxidant, and antineoplastic effects. Commonly, the antioxidant and anticancer properties of a drug are linked together as antioxidants scavenge free radicals and prevent the cellular DNA damage which could result in cancer development. In this investigation, comparative assessment of the anticancer and antioxidant potentials of methanolic seed extracts from two common varieties of MP,
Mucuna pruriens var. pruriens
(MPP) and
Mucuna pruriens var. utilis
(MPU) against human colorectal cancer adenocarcinoma cells COLO-205, was carried out. The highest antioxidant potential was recorded with MPP with an IC
50
of 45.71 μg/ml. The in vitro antiproliferative effects of MPP and MPU on COLO-205 showed an IC
50
of 131.1 μg/ml and 246.9 μg/ml respectively. Our results revealed intervention of the MPP and MPU extracts in growth kinetics of the COLO-205 cells in concomitance with apoptosis induction up to 8.73- and 5.58-folds respectively. The AO/EtBr dual staining and the flow cytometry results also confirmed the better apoptotic efficacy of MPP over MPU. MPP at a concentration of 160 μg/ml exhibited highest apoptosis and cell cycle arrest. Furthermore, effect of the seed extracts on p53 expression was investigated by quantitative RT-PCR and a maximum upregulation of 1.12-fold was recorded with MPP.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>37395947</pmid><doi>10.1007/s12010-023-04598-4</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0001-6994-5182</orcidid><orcidid>https://orcid.org/0000-0003-1319-7466</orcidid><orcidid>https://orcid.org/0000-0002-6545-1983</orcidid></addata></record> |
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| subjects | Adenocarcinoma Adenocarcinoma - drug therapy Anticancer properties Antineoplastic Agents - pharmacology Antioxidants Antioxidants - pharmacology Apoptosis Biochemistry Biotechnology Cancer Cell cycle Chemistry Chemistry and Materials Science Colorectal cancer Colorectal carcinoma Colorectal Neoplasms - drug therapy Damage prevention Diabetes mellitus DNA damage Fertility Flow cytometry Free radicals Growth kinetics Humans Legumes Male Movement disorders Mucuna Mucuna pruriens Neurodegenerative diseases Original Article Parkinson's disease Plant Extracts - pharmacology Seeds |
| title | Antineoplastic Effects of Mucuna pruriens Against Human Colorectal Adenocarcinoma |
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