Versatile methanotrophs form an active methane biofilter in the oxycline of a seasonally stratified coastal basin
The potential and drivers of microbial methane removal in the water column of seasonally stratified coastal ecosystems and the importance of the methanotrophic community composition for ecosystem functioning are not well explored. Here, we combined depth profiles of oxygen and methane with 16S rRNA...
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| Veröffentlicht in: | Environmental microbiology 2023-11, Vol.25 (11), p.2277-2288 |
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| creator | Venetz, Jessica Żygadłowska, Olga M. Lenstra, Wytze K. Helmond, Niels A. G. M. Nuijten, Guylaine H. L. Wallenius, Anna J. Dalcin Martins, Paula Slomp, Caroline P. Jetten, Mike S. M. Veraart, Annelies J. |
| description | The potential and drivers of microbial methane removal in the water column of seasonally stratified coastal ecosystems and the importance of the methanotrophic community composition for ecosystem functioning are not well explored. Here, we combined depth profiles of oxygen and methane with 16S rRNA gene amplicon sequencing, metagenomics and methane oxidation rates at discrete depths in a stratified coastal marine system (Lake Grevelingen, The Netherlands). Three amplicon sequence variants (ASVs) belonging to different genera of aerobic Methylomonadaceae and the corresponding three methanotrophic metagenome‐assembled genomes (MOB‐MAGs) were retrieved by 16S rRNA sequencing and metagenomic analysis, respectively. The abundances of the different methanotrophic ASVs and MOB‐MAGs peaked at different depths along the methane oxygen counter‐gradient and the MOB‐MAGs show a quite diverse genomic potential regarding oxygen metabolism, partial denitrification and sulphur metabolism. Moreover, potential aerobic methane oxidation rates indicated high methanotrophic activity throughout the methane oxygen counter‐gradient, even at depths with low in situ methane or oxygen concentration. This suggests that niche‐partitioning with high genomic versatility of the present Methylomonadaceae might contribute to the functional resilience of the methanotrophic community and ultimately the efficiency of methane removal in the stratified water column of a marine basin. |
| doi_str_mv | 10.1111/1462-2920.16448 |
| format | Article |
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G. M. ; Nuijten, Guylaine H. L. ; Wallenius, Anna J. ; Dalcin Martins, Paula ; Slomp, Caroline P. ; Jetten, Mike S. M. ; Veraart, Annelies J.</creator><creatorcontrib>Venetz, Jessica ; Żygadłowska, Olga M. ; Lenstra, Wytze K. ; Helmond, Niels A. G. M. ; Nuijten, Guylaine H. L. ; Wallenius, Anna J. ; Dalcin Martins, Paula ; Slomp, Caroline P. ; Jetten, Mike S. M. ; Veraart, Annelies J.</creatorcontrib><description>The potential and drivers of microbial methane removal in the water column of seasonally stratified coastal ecosystems and the importance of the methanotrophic community composition for ecosystem functioning are not well explored. Here, we combined depth profiles of oxygen and methane with 16S rRNA gene amplicon sequencing, metagenomics and methane oxidation rates at discrete depths in a stratified coastal marine system (Lake Grevelingen, The Netherlands). Three amplicon sequence variants (ASVs) belonging to different genera of aerobic Methylomonadaceae and the corresponding three methanotrophic metagenome‐assembled genomes (MOB‐MAGs) were retrieved by 16S rRNA sequencing and metagenomic analysis, respectively. The abundances of the different methanotrophic ASVs and MOB‐MAGs peaked at different depths along the methane oxygen counter‐gradient and the MOB‐MAGs show a quite diverse genomic potential regarding oxygen metabolism, partial denitrification and sulphur metabolism. Moreover, potential aerobic methane oxidation rates indicated high methanotrophic activity throughout the methane oxygen counter‐gradient, even at depths with low in situ methane or oxygen concentration. This suggests that niche‐partitioning with high genomic versatility of the present Methylomonadaceae might contribute to the functional resilience of the methanotrophic community and ultimately the efficiency of methane removal in the stratified water column of a marine basin.</description><identifier>ISSN: 1462-2912</identifier><identifier>EISSN: 1462-2920</identifier><identifier>DOI: 10.1111/1462-2920.16448</identifier><identifier>PMID: 37381163</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Biofilters ; Coastal ecosystems ; Community composition ; Concentration gradient ; Denitrification ; Depth ; Depth profiling ; Gene sequencing ; Genomes ; Genomics ; Lakes ; Metabolism ; Metagenomics ; Methane ; Methanotrophic bacteria ; Microorganisms ; Ocean basins ; Oxidation ; Oxygen ; Oxygen metabolism ; Removal ; rRNA 16S ; Sequencing ; Stratified water ; Sulphur ; Water circulation ; Water column</subject><ispartof>Environmental microbiology, 2023-11, Vol.25 (11), p.2277-2288</ispartof><rights>2023 The Authors. published by Applied Microbiology International and John Wiley & Sons Ltd.</rights><rights>2023 The Authors. 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Here, we combined depth profiles of oxygen and methane with 16S rRNA gene amplicon sequencing, metagenomics and methane oxidation rates at discrete depths in a stratified coastal marine system (Lake Grevelingen, The Netherlands). Three amplicon sequence variants (ASVs) belonging to different genera of aerobic Methylomonadaceae and the corresponding three methanotrophic metagenome‐assembled genomes (MOB‐MAGs) were retrieved by 16S rRNA sequencing and metagenomic analysis, respectively. The abundances of the different methanotrophic ASVs and MOB‐MAGs peaked at different depths along the methane oxygen counter‐gradient and the MOB‐MAGs show a quite diverse genomic potential regarding oxygen metabolism, partial denitrification and sulphur metabolism. Moreover, potential aerobic methane oxidation rates indicated high methanotrophic activity throughout the methane oxygen counter‐gradient, even at depths with low in situ methane or oxygen concentration. This suggests that niche‐partitioning with high genomic versatility of the present Methylomonadaceae might contribute to the functional resilience of the methanotrophic community and ultimately the efficiency of methane removal in the stratified water column of a marine basin.</description><subject>Biofilters</subject><subject>Coastal ecosystems</subject><subject>Community composition</subject><subject>Concentration gradient</subject><subject>Denitrification</subject><subject>Depth</subject><subject>Depth profiling</subject><subject>Gene sequencing</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Lakes</subject><subject>Metabolism</subject><subject>Metagenomics</subject><subject>Methane</subject><subject>Methanotrophic bacteria</subject><subject>Microorganisms</subject><subject>Ocean basins</subject><subject>Oxidation</subject><subject>Oxygen</subject><subject>Oxygen metabolism</subject><subject>Removal</subject><subject>rRNA 16S</subject><subject>Sequencing</subject><subject>Stratified water</subject><subject>Sulphur</subject><subject>Water circulation</subject><subject>Water column</subject><issn>1462-2912</issn><issn>1462-2920</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><recordid>eNqFkctPAyEQxonR-KievRkSL17W8thd4GhMfSQaL41XwrJsSsMuFaja_17qag9eJJkAM7_5kvkGgHOMrnE-U1zWpCCC5G9dlnwPHO8y-7s3JkfgJMYlQphRhg7BEWWUY1zTY_D2akJUyToDe5MWavAp-NUiws6HHqoBKp3s-2_RwMb6zrpkArQDTAsD_edGO5srvoMKRqOiH5RzGxhTyLqdNS3UXsWkHGxUtMMpOOiUi-bs556A-d1sfvtQPL3cP97ePBW6xIQXtUa0FUyYlnSiJqSpWsJEy1hVioYgTVmLKNFVWWpGK8MIxojhrqWoIbzidAKuRtlV8G9rE5PsbdTGuTyFX0dJOMVECMZpRi__oEu_DnmKLcVFyVGOTE1HSgcfYzCdXAXbq7CRGMntMuTWbrm1Xn4vI3dc_Oium960O_7X_QxUI_CR_d_8pydnz4-j8Be8BJMx</recordid><startdate>202311</startdate><enddate>202311</enddate><creator>Venetz, Jessica</creator><creator>Żygadłowska, Olga M.</creator><creator>Lenstra, Wytze K.</creator><creator>Helmond, Niels A. 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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Biofilters Coastal ecosystems Community composition Concentration gradient Denitrification Depth Depth profiling Gene sequencing Genomes Genomics Lakes Metabolism Metagenomics Methane Methanotrophic bacteria Microorganisms Ocean basins Oxidation Oxygen Oxygen metabolism Removal rRNA 16S Sequencing Stratified water Sulphur Water circulation Water column |
| title | Versatile methanotrophs form an active methane biofilter in the oxycline of a seasonally stratified coastal basin |
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