Isolation and characterization of the gene HvFAR1 encoding acyl‐CoA reductase from the cer‐za.227 mutant of barley (Hordeum vulgare) and analysis of the cuticular barrier functions
Summary The cuticle is a protective layer covering aerial plant organs. We studied the function of waxes for the establishment of the cuticular barrier in barley (Hordeum vulgare). The barley eceriferum mutants cer‐za.227 and cer‐ye.267 display reduced wax loads, but the genes affected, and the cons...
Gespeichert in:
| Veröffentlicht in: | The New phytologist 2023-09, Vol.239 (5), p.1903-1918 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1918 |
|---|---|
| container_issue | 5 |
| container_start_page | 1903 |
| container_title | The New phytologist |
| container_volume | 239 |
| creator | Müller, Yannic Patwari, Payal Stöcker, Tyll Zeisler‐Diehl, Viktoria Steiner, Ulrike Campoli, Chiara Grewe, Lea Kuczkowska, Magdalena Dierig, Maya Marita Jose, Sarah Hetherington, Alistair M. Acosta, Ivan F. Schoof, Heiko Schreiber, Lukas Dörmann, Peter |
| description | Summary
The cuticle is a protective layer covering aerial plant organs. We studied the function of waxes for the establishment of the cuticular barrier in barley (Hordeum vulgare). The barley eceriferum mutants cer‐za.227 and cer‐ye.267 display reduced wax loads, but the genes affected, and the consequences of the wax changes for the barrier function remained unknown.
Cuticular waxes and permeabilities were measured in cer‐za.227 and cer‐ye.267. The mutant loci were isolated by bulked segregant RNA sequencing. New cer‐za alleles were generated by genome editing. The CER‐ZA protein was characterized after expression in yeast and Arabidopsis cer4‐3.
Cer‐za.227 carries a mutation in HORVU5Hr1G089230 encoding acyl‐CoA reductase (FAR1). The cer‐ye.267 mutation is located to HORVU4Hr1G063420 encoding β‐ketoacyl‐CoA synthase (KAS1) and is allelic to cer‐zh.54. The amounts of intracuticular waxes were strongly decreased in cer‐ye.267. The cuticular water loss and permeability of cer‐za.227 were similar to wild‐type (WT), but were increased in cer‐ye.267. Removal of epicuticular waxes revealed that intracuticular, but not epicuticular waxes are required to regulate cuticular transpiration.
The differential decrease in intracuticular waxes between cer‐za.227 and cer‐ye.267, and the removal of epicuticular waxes indicate that the cuticular barrier function mostly depends on the presence of intracuticular waxes. |
| doi_str_mv | 10.1111/nph.19063 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2829430957</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2829430957</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3883-f968ba9fa8d90344e85a15f8b08ee04dcd4236b9d93091458d3505a61b0868e83</originalsourceid><addsrcrecordid>eNp1kcFuEzEQhi0EoqFw4AWQJS7tYVN77XXsYxS1pFIFCIHEbeXYs8lWu3aw10XpiUfgcXgengRvtuWAhC-WPN__z4x_hF5TMqf5XLj9bk4VEewJmlEuVCEpWzxFM0JKWQguvp6gFzHeEkJUJcrn6IQtGFdS8Bn6dR19p4fWO6ydxWangzYDhPZ-evQNHnaAt-AAr--ulp8oBme8bd0Wa3Pofv_4ufJLHMAmM-gIuAm-P0oMhFy81_OyXOA-DdoNo9tGhw4O-Gztg4XU47vUbXWA82N77XR3iG18bGvS0JrU6TDKQgsBN8mZcbD4Ej1rdBfh1cN9ir5cXX5erYubD--uV8ubwjApWdEoITdaNVpaRRjnICtNq0ZuiAQg3BrLSyY2yipGFOWVtKwilRY0A0KCZKfobPLdB_8tQRzqvo0Guk478CnWpSwVz9pqkdG3_6C3PoW80khxzisiyEidT5QJPsYATb0Pba_DoaakHuOsc5z1Mc7MvnlwTJse7F_yMb8MXEzA9zb_6v-d6vcf15PlH7ibrJU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2844450607</pqid></control><display><type>article</type><title>Isolation and characterization of the gene HvFAR1 encoding acyl‐CoA reductase from the cer‐za.227 mutant of barley (Hordeum vulgare) and analysis of the cuticular barrier functions</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Wiley Free Content</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Müller, Yannic ; Patwari, Payal ; Stöcker, Tyll ; Zeisler‐Diehl, Viktoria ; Steiner, Ulrike ; Campoli, Chiara ; Grewe, Lea ; Kuczkowska, Magdalena ; Dierig, Maya Marita ; Jose, Sarah ; Hetherington, Alistair M. ; Acosta, Ivan F. ; Schoof, Heiko ; Schreiber, Lukas ; Dörmann, Peter</creator><creatorcontrib>Müller, Yannic ; Patwari, Payal ; Stöcker, Tyll ; Zeisler‐Diehl, Viktoria ; Steiner, Ulrike ; Campoli, Chiara ; Grewe, Lea ; Kuczkowska, Magdalena ; Dierig, Maya Marita ; Jose, Sarah ; Hetherington, Alistair M. ; Acosta, Ivan F. ; Schoof, Heiko ; Schreiber, Lukas ; Dörmann, Peter</creatorcontrib><description>Summary
The cuticle is a protective layer covering aerial plant organs. We studied the function of waxes for the establishment of the cuticular barrier in barley (Hordeum vulgare). The barley eceriferum mutants cer‐za.227 and cer‐ye.267 display reduced wax loads, but the genes affected, and the consequences of the wax changes for the barrier function remained unknown.
Cuticular waxes and permeabilities were measured in cer‐za.227 and cer‐ye.267. The mutant loci were isolated by bulked segregant RNA sequencing. New cer‐za alleles were generated by genome editing. The CER‐ZA protein was characterized after expression in yeast and Arabidopsis cer4‐3.
Cer‐za.227 carries a mutation in HORVU5Hr1G089230 encoding acyl‐CoA reductase (FAR1). The cer‐ye.267 mutation is located to HORVU4Hr1G063420 encoding β‐ketoacyl‐CoA synthase (KAS1) and is allelic to cer‐zh.54. The amounts of intracuticular waxes were strongly decreased in cer‐ye.267. The cuticular water loss and permeability of cer‐za.227 were similar to wild‐type (WT), but were increased in cer‐ye.267. Removal of epicuticular waxes revealed that intracuticular, but not epicuticular waxes are required to regulate cuticular transpiration.
The differential decrease in intracuticular waxes between cer‐za.227 and cer‐ye.267, and the removal of epicuticular waxes indicate that the cuticular barrier function mostly depends on the presence of intracuticular waxes.</description><identifier>ISSN: 0028-646X</identifier><identifier>EISSN: 1469-8137</identifier><identifier>DOI: 10.1111/nph.19063</identifier><identifier>PMID: 37349864</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Alleles ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - metabolism ; Barley ; Body organs ; cuticle ; Cuticular transpiration ; Cuticular wax ; cutin ; Cyclin-Dependent Kinase Inhibitor Proteins - genetics ; Cyclin-Dependent Kinase Inhibitor Proteins - metabolism ; eceriferum ; Epicuticular wax ; Gene sequencing ; Genomes ; Hordeum - genetics ; Hordeum - metabolism ; Hordeum vulgare ; Mutants ; Mutation ; Mutation - genetics ; Nuclear Proteins - metabolism ; Permeability ; Plant Epidermis - metabolism ; Plant Leaves - metabolism ; Plant organs ; Reductases ; Removal ; RNA sequencing ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins - metabolism ; Transpiration ; Water - metabolism ; Water loss ; wax ; Waxes ; Waxes - metabolism ; Yeasts</subject><ispartof>The New phytologist, 2023-09, Vol.239 (5), p.1903-1918</ispartof><rights>2023 The Authors. © 2023 New Phytologist Foundation</rights><rights>2023 The Authors. New Phytologist © 2023 New Phytologist Foundation.</rights><rights>2023. This article is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3883-f968ba9fa8d90344e85a15f8b08ee04dcd4236b9d93091458d3505a61b0868e83</citedby><cites>FETCH-LOGICAL-c3883-f968ba9fa8d90344e85a15f8b08ee04dcd4236b9d93091458d3505a61b0868e83</cites><orcidid>0000-0001-7080-3384 ; 0000-0001-7593-7840 ; 0000-0001-7050-9716 ; 0000-0003-2737-6473 ; 0000-0002-6974-4951 ; 0000-0001-7809-2927 ; 0000-0003-1356-6639 ; 0000-0002-5845-9370 ; 0000-0001-7184-9472 ; 0000-0001-6060-9203 ; 0000-0001-7003-9929 ; 0000-0002-1527-3752</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fnph.19063$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fnph.19063$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>314,777,781,1412,1428,27905,27906,45555,45556,46390,46814</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37349864$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Müller, Yannic</creatorcontrib><creatorcontrib>Patwari, Payal</creatorcontrib><creatorcontrib>Stöcker, Tyll</creatorcontrib><creatorcontrib>Zeisler‐Diehl, Viktoria</creatorcontrib><creatorcontrib>Steiner, Ulrike</creatorcontrib><creatorcontrib>Campoli, Chiara</creatorcontrib><creatorcontrib>Grewe, Lea</creatorcontrib><creatorcontrib>Kuczkowska, Magdalena</creatorcontrib><creatorcontrib>Dierig, Maya Marita</creatorcontrib><creatorcontrib>Jose, Sarah</creatorcontrib><creatorcontrib>Hetherington, Alistair M.</creatorcontrib><creatorcontrib>Acosta, Ivan F.</creatorcontrib><creatorcontrib>Schoof, Heiko</creatorcontrib><creatorcontrib>Schreiber, Lukas</creatorcontrib><creatorcontrib>Dörmann, Peter</creatorcontrib><title>Isolation and characterization of the gene HvFAR1 encoding acyl‐CoA reductase from the cer‐za.227 mutant of barley (Hordeum vulgare) and analysis of the cuticular barrier functions</title><title>The New phytologist</title><addtitle>New Phytol</addtitle><description>Summary
The cuticle is a protective layer covering aerial plant organs. We studied the function of waxes for the establishment of the cuticular barrier in barley (Hordeum vulgare). The barley eceriferum mutants cer‐za.227 and cer‐ye.267 display reduced wax loads, but the genes affected, and the consequences of the wax changes for the barrier function remained unknown.
Cuticular waxes and permeabilities were measured in cer‐za.227 and cer‐ye.267. The mutant loci were isolated by bulked segregant RNA sequencing. New cer‐za alleles were generated by genome editing. The CER‐ZA protein was characterized after expression in yeast and Arabidopsis cer4‐3.
Cer‐za.227 carries a mutation in HORVU5Hr1G089230 encoding acyl‐CoA reductase (FAR1). The cer‐ye.267 mutation is located to HORVU4Hr1G063420 encoding β‐ketoacyl‐CoA synthase (KAS1) and is allelic to cer‐zh.54. The amounts of intracuticular waxes were strongly decreased in cer‐ye.267. The cuticular water loss and permeability of cer‐za.227 were similar to wild‐type (WT), but were increased in cer‐ye.267. Removal of epicuticular waxes revealed that intracuticular, but not epicuticular waxes are required to regulate cuticular transpiration.
The differential decrease in intracuticular waxes between cer‐za.227 and cer‐ye.267, and the removal of epicuticular waxes indicate that the cuticular barrier function mostly depends on the presence of intracuticular waxes.</description><subject>Alleles</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Barley</subject><subject>Body organs</subject><subject>cuticle</subject><subject>Cuticular transpiration</subject><subject>Cuticular wax</subject><subject>cutin</subject><subject>Cyclin-Dependent Kinase Inhibitor Proteins - genetics</subject><subject>Cyclin-Dependent Kinase Inhibitor Proteins - metabolism</subject><subject>eceriferum</subject><subject>Epicuticular wax</subject><subject>Gene sequencing</subject><subject>Genomes</subject><subject>Hordeum - genetics</subject><subject>Hordeum - metabolism</subject><subject>Hordeum vulgare</subject><subject>Mutants</subject><subject>Mutation</subject><subject>Mutation - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>Permeability</subject><subject>Plant Epidermis - metabolism</subject><subject>Plant Leaves - metabolism</subject><subject>Plant organs</subject><subject>Reductases</subject><subject>Removal</subject><subject>RNA sequencing</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Transpiration</subject><subject>Water - metabolism</subject><subject>Water loss</subject><subject>wax</subject><subject>Waxes</subject><subject>Waxes - metabolism</subject><subject>Yeasts</subject><issn>0028-646X</issn><issn>1469-8137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNp1kcFuEzEQhi0EoqFw4AWQJS7tYVN77XXsYxS1pFIFCIHEbeXYs8lWu3aw10XpiUfgcXgengRvtuWAhC-WPN__z4x_hF5TMqf5XLj9bk4VEewJmlEuVCEpWzxFM0JKWQguvp6gFzHeEkJUJcrn6IQtGFdS8Bn6dR19p4fWO6ydxWangzYDhPZ-evQNHnaAt-AAr--ulp8oBme8bd0Wa3Pofv_4ufJLHMAmM-gIuAm-P0oMhFy81_OyXOA-DdoNo9tGhw4O-Gztg4XU47vUbXWA82N77XR3iG18bGvS0JrU6TDKQgsBN8mZcbD4Ej1rdBfh1cN9ir5cXX5erYubD--uV8ubwjApWdEoITdaNVpaRRjnICtNq0ZuiAQg3BrLSyY2yipGFOWVtKwilRY0A0KCZKfobPLdB_8tQRzqvo0Guk478CnWpSwVz9pqkdG3_6C3PoW80khxzisiyEidT5QJPsYATb0Pba_DoaakHuOsc5z1Mc7MvnlwTJse7F_yMb8MXEzA9zb_6v-d6vcf15PlH7ibrJU</recordid><startdate>202309</startdate><enddate>202309</enddate><creator>Müller, Yannic</creator><creator>Patwari, Payal</creator><creator>Stöcker, Tyll</creator><creator>Zeisler‐Diehl, Viktoria</creator><creator>Steiner, Ulrike</creator><creator>Campoli, Chiara</creator><creator>Grewe, Lea</creator><creator>Kuczkowska, Magdalena</creator><creator>Dierig, Maya Marita</creator><creator>Jose, Sarah</creator><creator>Hetherington, Alistair M.</creator><creator>Acosta, Ivan F.</creator><creator>Schoof, Heiko</creator><creator>Schreiber, Lukas</creator><creator>Dörmann, Peter</creator><general>Wiley Subscription Services, Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7080-3384</orcidid><orcidid>https://orcid.org/0000-0001-7593-7840</orcidid><orcidid>https://orcid.org/0000-0001-7050-9716</orcidid><orcidid>https://orcid.org/0000-0003-2737-6473</orcidid><orcidid>https://orcid.org/0000-0002-6974-4951</orcidid><orcidid>https://orcid.org/0000-0001-7809-2927</orcidid><orcidid>https://orcid.org/0000-0003-1356-6639</orcidid><orcidid>https://orcid.org/0000-0002-5845-9370</orcidid><orcidid>https://orcid.org/0000-0001-7184-9472</orcidid><orcidid>https://orcid.org/0000-0001-6060-9203</orcidid><orcidid>https://orcid.org/0000-0001-7003-9929</orcidid><orcidid>https://orcid.org/0000-0002-1527-3752</orcidid></search><sort><creationdate>202309</creationdate><title>Isolation and characterization of the gene HvFAR1 encoding acyl‐CoA reductase from the cer‐za.227 mutant of barley (Hordeum vulgare) and analysis of the cuticular barrier functions</title><author>Müller, Yannic ; Patwari, Payal ; Stöcker, Tyll ; Zeisler‐Diehl, Viktoria ; Steiner, Ulrike ; Campoli, Chiara ; Grewe, Lea ; Kuczkowska, Magdalena ; Dierig, Maya Marita ; Jose, Sarah ; Hetherington, Alistair M. ; Acosta, Ivan F. ; Schoof, Heiko ; Schreiber, Lukas ; Dörmann, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3883-f968ba9fa8d90344e85a15f8b08ee04dcd4236b9d93091458d3505a61b0868e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Alleles</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Barley</topic><topic>Body organs</topic><topic>cuticle</topic><topic>Cuticular transpiration</topic><topic>Cuticular wax</topic><topic>cutin</topic><topic>Cyclin-Dependent Kinase Inhibitor Proteins - genetics</topic><topic>Cyclin-Dependent Kinase Inhibitor Proteins - metabolism</topic><topic>eceriferum</topic><topic>Epicuticular wax</topic><topic>Gene sequencing</topic><topic>Genomes</topic><topic>Hordeum - genetics</topic><topic>Hordeum - metabolism</topic><topic>Hordeum vulgare</topic><topic>Mutants</topic><topic>Mutation</topic><topic>Mutation - genetics</topic><topic>Nuclear Proteins - metabolism</topic><topic>Permeability</topic><topic>Plant Epidermis - metabolism</topic><topic>Plant Leaves - metabolism</topic><topic>Plant organs</topic><topic>Reductases</topic><topic>Removal</topic><topic>RNA sequencing</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>Transpiration</topic><topic>Water - metabolism</topic><topic>Water loss</topic><topic>wax</topic><topic>Waxes</topic><topic>Waxes - metabolism</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Müller, Yannic</creatorcontrib><creatorcontrib>Patwari, Payal</creatorcontrib><creatorcontrib>Stöcker, Tyll</creatorcontrib><creatorcontrib>Zeisler‐Diehl, Viktoria</creatorcontrib><creatorcontrib>Steiner, Ulrike</creatorcontrib><creatorcontrib>Campoli, Chiara</creatorcontrib><creatorcontrib>Grewe, Lea</creatorcontrib><creatorcontrib>Kuczkowska, Magdalena</creatorcontrib><creatorcontrib>Dierig, Maya Marita</creatorcontrib><creatorcontrib>Jose, Sarah</creatorcontrib><creatorcontrib>Hetherington, Alistair M.</creatorcontrib><creatorcontrib>Acosta, Ivan F.</creatorcontrib><creatorcontrib>Schoof, Heiko</creatorcontrib><creatorcontrib>Schreiber, Lukas</creatorcontrib><creatorcontrib>Dörmann, Peter</creatorcontrib><collection>Wiley-Blackwell Open Access Titles</collection><collection>Wiley Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The New phytologist</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Müller, Yannic</au><au>Patwari, Payal</au><au>Stöcker, Tyll</au><au>Zeisler‐Diehl, Viktoria</au><au>Steiner, Ulrike</au><au>Campoli, Chiara</au><au>Grewe, Lea</au><au>Kuczkowska, Magdalena</au><au>Dierig, Maya Marita</au><au>Jose, Sarah</au><au>Hetherington, Alistair M.</au><au>Acosta, Ivan F.</au><au>Schoof, Heiko</au><au>Schreiber, Lukas</au><au>Dörmann, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of the gene HvFAR1 encoding acyl‐CoA reductase from the cer‐za.227 mutant of barley (Hordeum vulgare) and analysis of the cuticular barrier functions</atitle><jtitle>The New phytologist</jtitle><addtitle>New Phytol</addtitle><date>2023-09</date><risdate>2023</risdate><volume>239</volume><issue>5</issue><spage>1903</spage><epage>1918</epage><pages>1903-1918</pages><issn>0028-646X</issn><eissn>1469-8137</eissn><abstract>Summary
The cuticle is a protective layer covering aerial plant organs. We studied the function of waxes for the establishment of the cuticular barrier in barley (Hordeum vulgare). The barley eceriferum mutants cer‐za.227 and cer‐ye.267 display reduced wax loads, but the genes affected, and the consequences of the wax changes for the barrier function remained unknown.
Cuticular waxes and permeabilities were measured in cer‐za.227 and cer‐ye.267. The mutant loci were isolated by bulked segregant RNA sequencing. New cer‐za alleles were generated by genome editing. The CER‐ZA protein was characterized after expression in yeast and Arabidopsis cer4‐3.
Cer‐za.227 carries a mutation in HORVU5Hr1G089230 encoding acyl‐CoA reductase (FAR1). The cer‐ye.267 mutation is located to HORVU4Hr1G063420 encoding β‐ketoacyl‐CoA synthase (KAS1) and is allelic to cer‐zh.54. The amounts of intracuticular waxes were strongly decreased in cer‐ye.267. The cuticular water loss and permeability of cer‐za.227 were similar to wild‐type (WT), but were increased in cer‐ye.267. Removal of epicuticular waxes revealed that intracuticular, but not epicuticular waxes are required to regulate cuticular transpiration.
The differential decrease in intracuticular waxes between cer‐za.227 and cer‐ye.267, and the removal of epicuticular waxes indicate that the cuticular barrier function mostly depends on the presence of intracuticular waxes.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>37349864</pmid><doi>10.1111/nph.19063</doi><tpages>1918</tpages><orcidid>https://orcid.org/0000-0001-7080-3384</orcidid><orcidid>https://orcid.org/0000-0001-7593-7840</orcidid><orcidid>https://orcid.org/0000-0001-7050-9716</orcidid><orcidid>https://orcid.org/0000-0003-2737-6473</orcidid><orcidid>https://orcid.org/0000-0002-6974-4951</orcidid><orcidid>https://orcid.org/0000-0001-7809-2927</orcidid><orcidid>https://orcid.org/0000-0003-1356-6639</orcidid><orcidid>https://orcid.org/0000-0002-5845-9370</orcidid><orcidid>https://orcid.org/0000-0001-7184-9472</orcidid><orcidid>https://orcid.org/0000-0001-6060-9203</orcidid><orcidid>https://orcid.org/0000-0001-7003-9929</orcidid><orcidid>https://orcid.org/0000-0002-1527-3752</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-646X |
| ispartof | The New phytologist, 2023-09, Vol.239 (5), p.1903-1918 |
| issn | 0028-646X 1469-8137 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2829430957 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Wiley Free Content; EZB-FREE-00999 freely available EZB journals |
| subjects | Alleles Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - metabolism Barley Body organs cuticle Cuticular transpiration Cuticular wax cutin Cyclin-Dependent Kinase Inhibitor Proteins - genetics Cyclin-Dependent Kinase Inhibitor Proteins - metabolism eceriferum Epicuticular wax Gene sequencing Genomes Hordeum - genetics Hordeum - metabolism Hordeum vulgare Mutants Mutation Mutation - genetics Nuclear Proteins - metabolism Permeability Plant Epidermis - metabolism Plant Leaves - metabolism Plant organs Reductases Removal RNA sequencing Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - metabolism Transpiration Water - metabolism Water loss wax Waxes Waxes - metabolism Yeasts |
| title | Isolation and characterization of the gene HvFAR1 encoding acyl‐CoA reductase from the cer‐za.227 mutant of barley (Hordeum vulgare) and analysis of the cuticular barrier functions |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T04%3A01%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Isolation%20and%20characterization%20of%20the%20gene%20HvFAR1%20encoding%20acyl%E2%80%90CoA%20reductase%20from%20the%20cer%E2%80%90za.227%20mutant%20of%20barley%20(Hordeum%20vulgare)%20and%20analysis%20of%20the%20cuticular%20barrier%20functions&rft.jtitle=The%20New%20phytologist&rft.au=M%C3%BCller,%20Yannic&rft.date=2023-09&rft.volume=239&rft.issue=5&rft.spage=1903&rft.epage=1918&rft.pages=1903-1918&rft.issn=0028-646X&rft.eissn=1469-8137&rft_id=info:doi/10.1111/nph.19063&rft_dat=%3Cproquest_cross%3E2829430957%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2844450607&rft_id=info:pmid/37349864&rfr_iscdi=true |