Distinct innate and adaptive immunity phenotypic profile at the circulating single-cell level in Psoriatic Arthritis

Mass cytometry was employed to investigate 47 circulating leukocyte subsets in patients with active psoriatic arthritis (PsA, n = 16) compared to healthy controls (n = 13), seropositive (RF and/or anti-CCP, n = 12) and seronegative (n = 9) RA patients. Comparing PsA to controls, different cell frequencies were found in both innate and adaptive immunity cell subsets, as well as in cells bridging innate and adaptive immunity. In some T cell subsets increased costimulatory molecules' expression in PsA, was also noted.No changes were observed in patients who remained disease-active after 3 months of treatment, in contrast to those who achieved remission/low-disease activity. Comparing PsA to seropositive RA, elevated frequencies of naïve and activated CD8+ T cells, B cells, MAIT/iNKT and ILCs were found, while the opposite was the case for terminal effector, senescent, and Th2-like cells. Strikingly, the composition of the leukocyte pool in PsA was comparable to seronegative RA, providing evidence for the pathogenetic similarities between these two entities. •Innate and adaptive immunity phenotypic profile by CyTOF is distinct in active PsA.•Circulating MAIT/iNKT and ILCs are increased in active PsA compared to healthy controls.•T and B cell compartments, MAIT/iNKT and ILCs differ between PsA and seropositive RA.•PsA is similar to seronegative RA in terms of circulating immunocyte phenotype.