Effect of IGFBP6 Knockdown on Proteins Regulating Exosome Synthesis and Secretion in MDA-MB-231 Cell Line
One of the potential causes of cancer recurrence is disruption of the cell—cell communication in the primary tumors that is realized, among other things, through secretion and uptake of exosomes by cells. Low expression of the IGFBP6 gene (insulin-like growth factor binding protein 6) is associated...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 2023-05, Vol.175 (1), p.157-161 |
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| creator | Efimova, A. S. Antipenko, I. D. Evtushenko, E. A. Balan, P. V. Tonevitskaya, S. A. |
| description | One of the potential causes of cancer recurrence is disruption of the cell—cell communication in the primary tumors that is realized, among other things, through secretion and uptake of exosomes by cells. Low expression of the
IGFBP6
gene (insulin-like growth factor binding protein 6) is associated with a high recurrence rate and can serve as a prognostic marker of luminal breast cancer. The knockdown of the
IGFBP6
gene leads to significant changes in lipid metabolism. We performed a quantitative analysis of both exosomes and proteins involved in the mechanism of their biogenesis. Changes in the expression profile of mRNAs and their proteins responsible for the synthesis and secretion of exosomes were revealed. We showed a decrease in the expression of the of the
VPS28
gene mRNA (vacuolar protein sorting-associated protein 28) and the corresponding protein by 2.3 and 5.6 times, respectively. The secretion of exosomes by MDA-MB-231 cells with
IGFBP6
knockdown decreased by 2 times. We discussed a mechanism of disruption of cell—cell communication. |
| doi_str_mv | 10.1007/s10517-023-05828-9 |
| format | Article |
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IGFBP6
gene (insulin-like growth factor binding protein 6) is associated with a high recurrence rate and can serve as a prognostic marker of luminal breast cancer. The knockdown of the
IGFBP6
gene leads to significant changes in lipid metabolism. We performed a quantitative analysis of both exosomes and proteins involved in the mechanism of their biogenesis. Changes in the expression profile of mRNAs and their proteins responsible for the synthesis and secretion of exosomes were revealed. We showed a decrease in the expression of the of the
VPS28
gene mRNA (vacuolar protein sorting-associated protein 28) and the corresponding protein by 2.3 and 5.6 times, respectively. The secretion of exosomes by MDA-MB-231 cells with
IGFBP6
knockdown decreased by 2 times. We discussed a mechanism of disruption of cell—cell communication.</description><identifier>ISSN: 0007-4888</identifier><identifier>EISSN: 1573-8221</identifier><identifier>DOI: 10.1007/s10517-023-05828-9</identifier><identifier>PMID: 37336811</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Analysis ; Biomedical and Life Sciences ; Biomedicine ; Breast cancer ; Cell Biology ; Cell interactions ; Cell Line, Tumor ; Ethylenediaminetetraacetic acid ; Exosomes ; Exosomes - genetics ; Exosomes - metabolism ; Gene Expression Regulation, Neoplastic ; Genes ; Health aspects ; Humans ; Insulin-Like Growth Factor Binding Protein 6 - genetics ; Insulin-Like Growth Factor Binding Protein 6 - metabolism ; Insulin-like growth factor-binding protein 6 ; Internal Medicine ; Laboratory Medicine ; Lipid metabolism ; MDA-MB-231 Cells ; mRNA ; Neoplasm Recurrence, Local - genetics ; Pathology ; Physiological aspects ; Protein binding ; Protein transport ; Proteins ; RNA ; Secretion</subject><ispartof>Bulletin of experimental biology and medicine, 2023-05, Vol.175 (1), p.157-161</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>COPYRIGHT 2023 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-227631b9b5c47c650b2fc4f786365e5a7dff6b3734ef574113491f59ed657b9c3</citedby><cites>FETCH-LOGICAL-c473t-227631b9b5c47c650b2fc4f786365e5a7dff6b3734ef574113491f59ed657b9c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10517-023-05828-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10517-023-05828-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37336811$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Efimova, A. S.</creatorcontrib><creatorcontrib>Antipenko, I. D.</creatorcontrib><creatorcontrib>Evtushenko, E. A.</creatorcontrib><creatorcontrib>Balan, P. V.</creatorcontrib><creatorcontrib>Tonevitskaya, S. A.</creatorcontrib><title>Effect of IGFBP6 Knockdown on Proteins Regulating Exosome Synthesis and Secretion in MDA-MB-231 Cell Line</title><title>Bulletin of experimental biology and medicine</title><addtitle>Bull Exp Biol Med</addtitle><addtitle>Bull Exp Biol Med</addtitle><description>One of the potential causes of cancer recurrence is disruption of the cell—cell communication in the primary tumors that is realized, among other things, through secretion and uptake of exosomes by cells. Low expression of the
IGFBP6
gene (insulin-like growth factor binding protein 6) is associated with a high recurrence rate and can serve as a prognostic marker of luminal breast cancer. The knockdown of the
IGFBP6
gene leads to significant changes in lipid metabolism. We performed a quantitative analysis of both exosomes and proteins involved in the mechanism of their biogenesis. Changes in the expression profile of mRNAs and their proteins responsible for the synthesis and secretion of exosomes were revealed. We showed a decrease in the expression of the of the
VPS28
gene mRNA (vacuolar protein sorting-associated protein 28) and the corresponding protein by 2.3 and 5.6 times, respectively. The secretion of exosomes by MDA-MB-231 cells with
IGFBP6
knockdown decreased by 2 times. We discussed a mechanism of disruption of cell—cell communication.</description><subject>Analysis</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Breast cancer</subject><subject>Cell Biology</subject><subject>Cell interactions</subject><subject>Cell Line, Tumor</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Exosomes</subject><subject>Exosomes - genetics</subject><subject>Exosomes - metabolism</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genes</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Insulin-Like Growth Factor Binding Protein 6 - genetics</subject><subject>Insulin-Like Growth Factor Binding Protein 6 - metabolism</subject><subject>Insulin-like growth factor-binding protein 6</subject><subject>Internal Medicine</subject><subject>Laboratory Medicine</subject><subject>Lipid metabolism</subject><subject>MDA-MB-231 Cells</subject><subject>mRNA</subject><subject>Neoplasm Recurrence, Local - genetics</subject><subject>Pathology</subject><subject>Physiological aspects</subject><subject>Protein binding</subject><subject>Protein transport</subject><subject>Proteins</subject><subject>RNA</subject><subject>Secretion</subject><issn>0007-4888</issn><issn>1573-8221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kktv1DAUhS0EokPhD7BAlpAQmxQ_4keW02FaKqaiorC2Eud6xiWxS5wI-u9xmEIpQsgL69rfObrXPgg9p-SIEqLeJEoEVQVhvCBCM11UD9CCCsULzRh9iBYkU0WptT5AT1K6mksi6WN0wBXnUlO6QH7tHNgRR4fPTk-OLyR-H6L90sZvAceAL4Y4gg8Jf4Tt1NWjD1u8_h5T7AFf3oRxB8knXIcWX4IdYPRZ4wM-f7sszo8LxileQdfhjQ_wFD1ydZfg2e1-iD6frD-t3hWbD6dnq-WmsKXiY8GYkpw2VSNybaUgDXO2dEpLLgWIWrXOySYPUIITqqSUlxV1ooJWCtVUlh-i13vf6yF-nSCNpvfJ5i7qAHFKhmmmKsYk1Rl9-Rd6Fach5O5mqhJKl4rdUdu6A-ODi-NQ29nULJWkFdVCVZk6-geVVwu9tzGA8_n8nuDVH4Id1N24S7Gb5jdM90G2B-0QUxrAmevB9_VwYygxcxDMPggmB8H8DIKZRS9uR5uaHtrfkl8_nwG-B1K-ClsY7mb_j-0PX_G4UQ</recordid><startdate>20230501</startdate><enddate>20230501</enddate><creator>Efimova, A. 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S. ; Antipenko, I. D. ; Evtushenko, E. A. ; Balan, P. V. ; Tonevitskaya, S. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-227631b9b5c47c650b2fc4f786365e5a7dff6b3734ef574113491f59ed657b9c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Analysis</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Breast cancer</topic><topic>Cell Biology</topic><topic>Cell interactions</topic><topic>Cell Line, Tumor</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Exosomes</topic><topic>Exosomes - genetics</topic><topic>Exosomes - metabolism</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genes</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Insulin-Like Growth Factor Binding Protein 6 - genetics</topic><topic>Insulin-Like Growth Factor Binding Protein 6 - metabolism</topic><topic>Insulin-like growth factor-binding protein 6</topic><topic>Internal Medicine</topic><topic>Laboratory Medicine</topic><topic>Lipid metabolism</topic><topic>MDA-MB-231 Cells</topic><topic>mRNA</topic><topic>Neoplasm Recurrence, Local - genetics</topic><topic>Pathology</topic><topic>Physiological aspects</topic><topic>Protein binding</topic><topic>Protein transport</topic><topic>Proteins</topic><topic>RNA</topic><topic>Secretion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Efimova, A. 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S.</au><au>Antipenko, I. D.</au><au>Evtushenko, E. A.</au><au>Balan, P. V.</au><au>Tonevitskaya, S. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of IGFBP6 Knockdown on Proteins Regulating Exosome Synthesis and Secretion in MDA-MB-231 Cell Line</atitle><jtitle>Bulletin of experimental biology and medicine</jtitle><stitle>Bull Exp Biol Med</stitle><addtitle>Bull Exp Biol Med</addtitle><date>2023-05-01</date><risdate>2023</risdate><volume>175</volume><issue>1</issue><spage>157</spage><epage>161</epage><pages>157-161</pages><issn>0007-4888</issn><eissn>1573-8221</eissn><abstract>One of the potential causes of cancer recurrence is disruption of the cell—cell communication in the primary tumors that is realized, among other things, through secretion and uptake of exosomes by cells. Low expression of the
IGFBP6
gene (insulin-like growth factor binding protein 6) is associated with a high recurrence rate and can serve as a prognostic marker of luminal breast cancer. The knockdown of the
IGFBP6
gene leads to significant changes in lipid metabolism. We performed a quantitative analysis of both exosomes and proteins involved in the mechanism of their biogenesis. Changes in the expression profile of mRNAs and their proteins responsible for the synthesis and secretion of exosomes were revealed. We showed a decrease in the expression of the of the
VPS28
gene mRNA (vacuolar protein sorting-associated protein 28) and the corresponding protein by 2.3 and 5.6 times, respectively. The secretion of exosomes by MDA-MB-231 cells with
IGFBP6
knockdown decreased by 2 times. We discussed a mechanism of disruption of cell—cell communication.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>37336811</pmid><doi>10.1007/s10517-023-05828-9</doi><tpages>5</tpages></addata></record> |
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| subjects | Analysis Biomedical and Life Sciences Biomedicine Breast cancer Cell Biology Cell interactions Cell Line, Tumor Ethylenediaminetetraacetic acid Exosomes Exosomes - genetics Exosomes - metabolism Gene Expression Regulation, Neoplastic Genes Health aspects Humans Insulin-Like Growth Factor Binding Protein 6 - genetics Insulin-Like Growth Factor Binding Protein 6 - metabolism Insulin-like growth factor-binding protein 6 Internal Medicine Laboratory Medicine Lipid metabolism MDA-MB-231 Cells mRNA Neoplasm Recurrence, Local - genetics Pathology Physiological aspects Protein binding Protein transport Proteins RNA Secretion |
| title | Effect of IGFBP6 Knockdown on Proteins Regulating Exosome Synthesis and Secretion in MDA-MB-231 Cell Line |
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