Detection of androgenic activity in emissions from diesel fuel and biomass combustion
The present study evaluated both diesel fuel exhaust and biomass (wood) burn extracts for androgen receptor–mediated activity using MDA‐kb2 cells, which contain an androgen‐responsive promoter‐luciferase reporter gene construct. This assay and analytical fractionization of the samples were used as t...
Gespeichert in:
| Veröffentlicht in: | Environmental toxicology and chemistry 2006-08, Vol.25 (8), p.2123-2131 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2131 |
|---|---|
| container_issue | 8 |
| container_start_page | 2123 |
| container_title | Environmental toxicology and chemistry |
| container_volume | 25 |
| creator | Owens Jr, Clyde V. Lambright, Christy Cardon, Mary Gray Jr, L. Earl Gullett, Brian K. Wilson, Vickie S. |
| description | The present study evaluated both diesel fuel exhaust and biomass (wood) burn extracts for androgen receptor–mediated activity using MDA‐kb2 cells, which contain an androgen‐responsive promoter‐luciferase reporter gene construct. This assay and analytical fractionization of the samples were used as tools to separate active from inactive fractions, with the goal of identifying the specific compounds responsible for the activity. A significant androgenic response was detected from the diesel emission. High‐performance liquid chromatographic fractionation of the sample indicated that significant androgenic activity was retained in three fractions. 4‐Hydroxybiphenyl was identified from the most active fraction using gas chromatography/mass spectroscopy. This purified compound was then tested at doses from 1 nM to 100 μM. 4‐Hydroxybiphenol exhibited antagonist activity at low concentrations and agonist activity at high concentrations. A competitive‐binding assay confirmed binding to the androgen receptor, with a median inhibitory concentration for radioligand binding of approximately 370 nM. Significant androgenic activity also was detected in the wood burn samples, but we were unable to identify the specific chemicals responsible for this endocrine activity. The present study demonstrates that in vitro bioassays can serve as sensitive bioanalytical tools to aid in characterization of complex environmental mixtures. |
| doi_str_mv | 10.1897/05-551R.1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_28258521</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>19339446</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5161-ce66f45e44124547792cfdb8987d1b658e9ddb2111f829b416d7f82915a50a5a3</originalsourceid><addsrcrecordid>eNqF0V9rFDEQAPAgij2rD34BWQQFH7Zmspn8eSytVsupIFYfQzabldTdTU12be_bm_MOC4L0JRPCbyaTDCFPgR6B0vI1xRoRPh_BPbICRFYrAeo-WVHZ0FoyoQ7Io5wvKQWhtX5IDkoEQRu2IhenfvZuDnGqYl_ZqUvxu5-Cq2w5_BXmTRWmyo8h50Jy1ac4Vl3w2Q9Vv5SlZFRtiKPNuXJxbJe8rfWYPOjtkP2TfTwkF2_ffDl5V68_nb0_OV7XDkFA7bwQPUfPOTCOXErNXN-1SivZQStQed11LQOAXjHdchCd3O4ALVKLtjkkL3d1r1L8ufg8m9Kp88NgJx-XbJhiqJDBnRB002jOxd2QS4XImwKf_wMv45Km8lrDgDZCSy0LerVDLsWck-_NVQqjTRsD1GxHZyia7ejMtsVn-4JLO_ruVu5nVcCLPbDZ2aFPdnIh3zqptWQKi-M7dx0Gv_n_jaYgFIwypOrPF9W7tJBnf_M3zaYfRshGovn28czQU_j64XwN5rz5DcznvSw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>210369797</pqid></control><display><type>article</type><title>Detection of androgenic activity in emissions from diesel fuel and biomass combustion</title><source>MEDLINE</source><source>Wiley Journals</source><creator>Owens Jr, Clyde V. ; Lambright, Christy ; Cardon, Mary ; Gray Jr, L. Earl ; Gullett, Brian K. ; Wilson, Vickie S.</creator><creatorcontrib>Owens Jr, Clyde V. ; Lambright, Christy ; Cardon, Mary ; Gray Jr, L. Earl ; Gullett, Brian K. ; Wilson, Vickie S.</creatorcontrib><description>The present study evaluated both diesel fuel exhaust and biomass (wood) burn extracts for androgen receptor–mediated activity using MDA‐kb2 cells, which contain an androgen‐responsive promoter‐luciferase reporter gene construct. This assay and analytical fractionization of the samples were used as tools to separate active from inactive fractions, with the goal of identifying the specific compounds responsible for the activity. A significant androgenic response was detected from the diesel emission. High‐performance liquid chromatographic fractionation of the sample indicated that significant androgenic activity was retained in three fractions. 4‐Hydroxybiphenyl was identified from the most active fraction using gas chromatography/mass spectroscopy. This purified compound was then tested at doses from 1 nM to 100 μM. 4‐Hydroxybiphenol exhibited antagonist activity at low concentrations and agonist activity at high concentrations. A competitive‐binding assay confirmed binding to the androgen receptor, with a median inhibitory concentration for radioligand binding of approximately 370 nM. Significant androgenic activity also was detected in the wood burn samples, but we were unable to identify the specific chemicals responsible for this endocrine activity. The present study demonstrates that in vitro bioassays can serve as sensitive bioanalytical tools to aid in characterization of complex environmental mixtures.</description><identifier>ISSN: 0730-7268</identifier><identifier>EISSN: 1552-8618</identifier><identifier>DOI: 10.1897/05-551R.1</identifier><identifier>PMID: 16916032</identifier><identifier>CODEN: ETOCDK</identifier><language>eng</language><publisher>Hoboken: Wiley Periodicals, Inc</publisher><subject>Androgenic activity ; Androgens ; Androgens - analysis ; Androgens - metabolism ; Androgens - pharmacology ; Animal, plant and microbial ecology ; Animals ; Applied ecology ; Binding, Competitive ; Bioassays ; Biological and medical sciences ; Biomass ; By products ; Cell Line ; Cells ; Cercopithecus aethiops ; Chromatography, High Pressure Liquid ; COS Cells ; Diesel exhaust ; Diesel fuels ; Dose-Response Relationship, Drug ; Ecotoxicology, biological effects of pollution ; Emissions ; Endocrine disruptors ; Endocrine Disruptors - analysis ; Endocrine Disruptors - metabolism ; Fractionation ; Fundamental and applied biological sciences. Psychology ; Gas chromatography ; Gas Chromatography-Mass Spectrometry ; Gases ; Gene expression ; General aspects ; Humans ; Mass spectroscopy ; Receptors, Androgen - metabolism ; Reproductive system ; Samples ; Vehicle Emissions - analysis</subject><ispartof>Environmental toxicology and chemistry, 2006-08, Vol.25 (8), p.2123-2131</ispartof><rights>Copyright © 2006 SETAC</rights><rights>2006 INIST-CNRS</rights><rights>Copyright Alliance Communications Group, A Division of Allen Press, Inc. Aug 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5161-ce66f45e44124547792cfdb8987d1b658e9ddb2111f829b416d7f82915a50a5a3</citedby><cites>FETCH-LOGICAL-c5161-ce66f45e44124547792cfdb8987d1b658e9ddb2111f829b416d7f82915a50a5a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1897%2F05-551R.1$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1897%2F05-551R.1$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17997285$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16916032$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Owens Jr, Clyde V.</creatorcontrib><creatorcontrib>Lambright, Christy</creatorcontrib><creatorcontrib>Cardon, Mary</creatorcontrib><creatorcontrib>Gray Jr, L. Earl</creatorcontrib><creatorcontrib>Gullett, Brian K.</creatorcontrib><creatorcontrib>Wilson, Vickie S.</creatorcontrib><title>Detection of androgenic activity in emissions from diesel fuel and biomass combustion</title><title>Environmental toxicology and chemistry</title><addtitle>Environmental Toxicology and Chemistry</addtitle><description>The present study evaluated both diesel fuel exhaust and biomass (wood) burn extracts for androgen receptor–mediated activity using MDA‐kb2 cells, which contain an androgen‐responsive promoter‐luciferase reporter gene construct. This assay and analytical fractionization of the samples were used as tools to separate active from inactive fractions, with the goal of identifying the specific compounds responsible for the activity. A significant androgenic response was detected from the diesel emission. High‐performance liquid chromatographic fractionation of the sample indicated that significant androgenic activity was retained in three fractions. 4‐Hydroxybiphenyl was identified from the most active fraction using gas chromatography/mass spectroscopy. This purified compound was then tested at doses from 1 nM to 100 μM. 4‐Hydroxybiphenol exhibited antagonist activity at low concentrations and agonist activity at high concentrations. A competitive‐binding assay confirmed binding to the androgen receptor, with a median inhibitory concentration for radioligand binding of approximately 370 nM. Significant androgenic activity also was detected in the wood burn samples, but we were unable to identify the specific chemicals responsible for this endocrine activity. The present study demonstrates that in vitro bioassays can serve as sensitive bioanalytical tools to aid in characterization of complex environmental mixtures.</description><subject>Androgenic activity</subject><subject>Androgens</subject><subject>Androgens - analysis</subject><subject>Androgens - metabolism</subject><subject>Androgens - pharmacology</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Applied ecology</subject><subject>Binding, Competitive</subject><subject>Bioassays</subject><subject>Biological and medical sciences</subject><subject>Biomass</subject><subject>By products</subject><subject>Cell Line</subject><subject>Cells</subject><subject>Cercopithecus aethiops</subject><subject>Chromatography, High Pressure Liquid</subject><subject>COS Cells</subject><subject>Diesel exhaust</subject><subject>Diesel fuels</subject><subject>Dose-Response Relationship, Drug</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Emissions</subject><subject>Endocrine disruptors</subject><subject>Endocrine Disruptors - analysis</subject><subject>Endocrine Disruptors - metabolism</subject><subject>Fractionation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gas chromatography</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Gases</subject><subject>Gene expression</subject><subject>General aspects</subject><subject>Humans</subject><subject>Mass spectroscopy</subject><subject>Receptors, Androgen - metabolism</subject><subject>Reproductive system</subject><subject>Samples</subject><subject>Vehicle Emissions - analysis</subject><issn>0730-7268</issn><issn>1552-8618</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0V9rFDEQAPAgij2rD34BWQQFH7Zmspn8eSytVsupIFYfQzabldTdTU12be_bm_MOC4L0JRPCbyaTDCFPgR6B0vI1xRoRPh_BPbICRFYrAeo-WVHZ0FoyoQ7Io5wvKQWhtX5IDkoEQRu2IhenfvZuDnGqYl_ZqUvxu5-Cq2w5_BXmTRWmyo8h50Jy1ac4Vl3w2Q9Vv5SlZFRtiKPNuXJxbJe8rfWYPOjtkP2TfTwkF2_ffDl5V68_nb0_OV7XDkFA7bwQPUfPOTCOXErNXN-1SivZQStQed11LQOAXjHdchCd3O4ALVKLtjkkL3d1r1L8ufg8m9Kp88NgJx-XbJhiqJDBnRB002jOxd2QS4XImwKf_wMv45Km8lrDgDZCSy0LerVDLsWck-_NVQqjTRsD1GxHZyia7ejMtsVn-4JLO_ruVu5nVcCLPbDZ2aFPdnIh3zqptWQKi-M7dx0Gv_n_jaYgFIwypOrPF9W7tJBnf_M3zaYfRshGovn28czQU_j64XwN5rz5DcznvSw</recordid><startdate>200608</startdate><enddate>200608</enddate><creator>Owens Jr, Clyde V.</creator><creator>Lambright, Christy</creator><creator>Cardon, Mary</creator><creator>Gray Jr, L. Earl</creator><creator>Gullett, Brian K.</creator><creator>Wilson, Vickie S.</creator><general>Wiley Periodicals, Inc</general><general>SETAC</general><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>L6V</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7TV</scope><scope>F28</scope><scope>KR7</scope></search><sort><creationdate>200608</creationdate><title>Detection of androgenic activity in emissions from diesel fuel and biomass combustion</title><author>Owens Jr, Clyde V. ; Lambright, Christy ; Cardon, Mary ; Gray Jr, L. Earl ; Gullett, Brian K. ; Wilson, Vickie S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5161-ce66f45e44124547792cfdb8987d1b658e9ddb2111f829b416d7f82915a50a5a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Androgenic activity</topic><topic>Androgens</topic><topic>Androgens - analysis</topic><topic>Androgens - metabolism</topic><topic>Androgens - pharmacology</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Applied ecology</topic><topic>Binding, Competitive</topic><topic>Bioassays</topic><topic>Biological and medical sciences</topic><topic>Biomass</topic><topic>By products</topic><topic>Cell Line</topic><topic>Cells</topic><topic>Cercopithecus aethiops</topic><topic>Chromatography, High Pressure Liquid</topic><topic>COS Cells</topic><topic>Diesel exhaust</topic><topic>Diesel fuels</topic><topic>Dose-Response Relationship, Drug</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>Emissions</topic><topic>Endocrine disruptors</topic><topic>Endocrine Disruptors - analysis</topic><topic>Endocrine Disruptors - metabolism</topic><topic>Fractionation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gas chromatography</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Gases</topic><topic>Gene expression</topic><topic>General aspects</topic><topic>Humans</topic><topic>Mass spectroscopy</topic><topic>Receptors, Androgen - metabolism</topic><topic>Reproductive system</topic><topic>Samples</topic><topic>Vehicle Emissions - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Owens Jr, Clyde V.</creatorcontrib><creatorcontrib>Lambright, Christy</creatorcontrib><creatorcontrib>Cardon, Mary</creatorcontrib><creatorcontrib>Gray Jr, L. Earl</creatorcontrib><creatorcontrib>Gullett, Brian K.</creatorcontrib><creatorcontrib>Wilson, Vickie S.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>ProQuest Engineering Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>Engineering Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>Pollution Abstracts</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Civil Engineering Abstracts</collection><jtitle>Environmental toxicology and chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Owens Jr, Clyde V.</au><au>Lambright, Christy</au><au>Cardon, Mary</au><au>Gray Jr, L. Earl</au><au>Gullett, Brian K.</au><au>Wilson, Vickie S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of androgenic activity in emissions from diesel fuel and biomass combustion</atitle><jtitle>Environmental toxicology and chemistry</jtitle><addtitle>Environmental Toxicology and Chemistry</addtitle><date>2006-08</date><risdate>2006</risdate><volume>25</volume><issue>8</issue><spage>2123</spage><epage>2131</epage><pages>2123-2131</pages><issn>0730-7268</issn><eissn>1552-8618</eissn><coden>ETOCDK</coden><abstract>The present study evaluated both diesel fuel exhaust and biomass (wood) burn extracts for androgen receptor–mediated activity using MDA‐kb2 cells, which contain an androgen‐responsive promoter‐luciferase reporter gene construct. This assay and analytical fractionization of the samples were used as tools to separate active from inactive fractions, with the goal of identifying the specific compounds responsible for the activity. A significant androgenic response was detected from the diesel emission. High‐performance liquid chromatographic fractionation of the sample indicated that significant androgenic activity was retained in three fractions. 4‐Hydroxybiphenyl was identified from the most active fraction using gas chromatography/mass spectroscopy. This purified compound was then tested at doses from 1 nM to 100 μM. 4‐Hydroxybiphenol exhibited antagonist activity at low concentrations and agonist activity at high concentrations. A competitive‐binding assay confirmed binding to the androgen receptor, with a median inhibitory concentration for radioligand binding of approximately 370 nM. Significant androgenic activity also was detected in the wood burn samples, but we were unable to identify the specific chemicals responsible for this endocrine activity. The present study demonstrates that in vitro bioassays can serve as sensitive bioanalytical tools to aid in characterization of complex environmental mixtures.</abstract><cop>Hoboken</cop><pub>Wiley Periodicals, Inc</pub><pmid>16916032</pmid><doi>10.1897/05-551R.1</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0730-7268 |
| ispartof | Environmental toxicology and chemistry, 2006-08, Vol.25 (8), p.2123-2131 |
| issn | 0730-7268 1552-8618 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_28258521 |
| source | MEDLINE; Wiley Journals |
| subjects | Androgenic activity Androgens Androgens - analysis Androgens - metabolism Androgens - pharmacology Animal, plant and microbial ecology Animals Applied ecology Binding, Competitive Bioassays Biological and medical sciences Biomass By products Cell Line Cells Cercopithecus aethiops Chromatography, High Pressure Liquid COS Cells Diesel exhaust Diesel fuels Dose-Response Relationship, Drug Ecotoxicology, biological effects of pollution Emissions Endocrine disruptors Endocrine Disruptors - analysis Endocrine Disruptors - metabolism Fractionation Fundamental and applied biological sciences. Psychology Gas chromatography Gas Chromatography-Mass Spectrometry Gases Gene expression General aspects Humans Mass spectroscopy Receptors, Androgen - metabolism Reproductive system Samples Vehicle Emissions - analysis |
| title | Detection of androgenic activity in emissions from diesel fuel and biomass combustion |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T13%3A45%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Detection%20of%20androgenic%20activity%20in%20emissions%20from%20diesel%20fuel%20and%20biomass%20combustion&rft.jtitle=Environmental%20toxicology%20and%20chemistry&rft.au=Owens%20Jr,%20Clyde%20V.&rft.date=2006-08&rft.volume=25&rft.issue=8&rft.spage=2123&rft.epage=2131&rft.pages=2123-2131&rft.issn=0730-7268&rft.eissn=1552-8618&rft.coden=ETOCDK&rft_id=info:doi/10.1897/05-551R.1&rft_dat=%3Cproquest_cross%3E19339446%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=210369797&rft_id=info:pmid/16916032&rfr_iscdi=true |