Efficient methods for multiple types of precise gene‐editing in Chlamydomonas

SUMMARY Precise gene‐editing using CRISPR/Cas9 technology remains a long‐standing challenge, especially for genes with low expression and no selectable phenotypes in Chlamydomonas reinhardtii, a classic model for photosynthesis and cilia research. Here, we developed a multi‐type and precise genetic...

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Veröffentlicht in:	The Plant journal : for cell and molecular biology 2023-08, Vol.115 (3), p.846-865
Hauptverfasser:	Chen, Hui, Yang, Qing‐Lin, Xu, Jia‐Xi, Deng, Xuan, Zhang, Yun‐Jie, Liu, Ting, Rots, Marianne G., Xu, Guo‐Liang, Huang, Kai‐Yao
Format:	Artikel
Sprache:	eng
Schlagworte:	3' Untranslated regions Amino acid substitution Amino acids Chlamydomonas Cilia CRISPR Deoxyribonucleic acid DNA DNA damage Editing efficient Epitopes Gene deletion Gene expression Genes Genetic engineering Genetic modification gene‐editing homology‐mediated Inactivation Industrial applications multi‐type Nuclease Phenotypes Photosynthesis precision Substitutes
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