Knockout of Erbin promotes pyroptosis via regulating NLRP3/caspase-1/Gasdermin D pathway in sepsis-induced acute kidney injury
Background This study aims to investigate the correlation between Erbin and sepsis, and the role of Erbin on the pyroptosis pathway in acute kidney injury caused by sepsis and NLRP3/caspase-1/Gasdermin D pathway. Methods In the study, lipopolysaccharide (LPS) treatment or cecal ligation and puncture...
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| Veröffentlicht in: | Clinical and experimental nephrology 2023-09, Vol.27 (9), p.781-790 |
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| container_title | Clinical and experimental nephrology |
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| creator | Liu, Yuping Fang, Qing Ming, Tingqian Zuo, Jing Jing, Guoqing Song, Xuemin |
| description | Background
This study aims to investigate the correlation between Erbin and sepsis, and the role of Erbin on the pyroptosis pathway in acute kidney injury caused by sepsis and NLRP3/caspase-1/Gasdermin D pathway.
Methods
In the study, lipopolysaccharide (LPS) treatment or cecal ligation and puncture (CLP) surgery on mice were used to stimulate the in vitro and in vivo sepsis-induced renal injury model. The male C57BL/6 of wild-type mice (WT) and Erbin-knockout mice (Erbin
−/−
, EKO) were randomly divided into four groups (WT + Sham, WT + CLP, EKO + Sham, EKO + CLP). Inflammatory cytokine, renal function, pyroptotic cell numbers and the levels of protein and mRNA expression of pyroptosis, including the NLRP3 (all
P
|
| doi_str_mv | 10.1007/s10157-023-02364-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2825502428</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2825502428</sourcerecordid><originalsourceid>FETCH-LOGICAL-c350t-7c35c4a6222f7fdb2114853ab3ff70ff106a24c62f37988ed31c615a2f2000b33</originalsourceid><addsrcrecordid>eNp9kU9v1DAQxa2KipbCF-gBWeLCxaz_xLFzrEppK1YtQnC2HMdest3EwROD9sJnr5ctrcShB2tsze-98eghdMroB0apWgCjTCpCudiduiL6AB2zSiiiVNO8KHdRccKUZEfoFcCaUqob2bxER0IJRmXdHKM_n8fo7mKecQz4IrX9iKcUhzh7wNM2xWmO0AP-1Vuc_Cpv7NyPK3yz_PpFLJyFyYInbHFpofNpKOKPeLLzj992i8sD_FTEpB-77HyHrcuzx3d9N_pde53T9jU6DHYD_s1DPUHfP118O78iy9vL6_OzJXFC0pmoUlxla855UKFrOWOVlsK2IgRFQ2C0trxyNQ9CNVr7TjBXM2l54GXpVogT9H7vW5b7mT3MZujB-c3Gjj5mMFxzKSmvuC7ou__QdcxpLL8rVEOF0Jo2heJ7yqUIkHwwU-oHm7aGUbNLx-zTMSUZ8zcds7N--2Cd28F3j5J_cRRA7AEorXHl09PsZ2zvAaDSmqw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2890338809</pqid></control><display><type>article</type><title>Knockout of Erbin promotes pyroptosis via regulating NLRP3/caspase-1/Gasdermin D pathway in sepsis-induced acute kidney injury</title><source>SpringerLink Journals</source><creator>Liu, Yuping ; Fang, Qing ; Ming, Tingqian ; Zuo, Jing ; Jing, Guoqing ; Song, Xuemin</creator><creatorcontrib>Liu, Yuping ; Fang, Qing ; Ming, Tingqian ; Zuo, Jing ; Jing, Guoqing ; Song, Xuemin</creatorcontrib><description>Background
This study aims to investigate the correlation between Erbin and sepsis, and the role of Erbin on the pyroptosis pathway in acute kidney injury caused by sepsis and NLRP3/caspase-1/Gasdermin D pathway.
Methods
In the study, lipopolysaccharide (LPS) treatment or cecal ligation and puncture (CLP) surgery on mice were used to stimulate the in vitro and in vivo sepsis-induced renal injury model. The male C57BL/6 of wild-type mice (WT) and Erbin-knockout mice (Erbin
−/−
, EKO) were randomly divided into four groups (WT + Sham, WT + CLP, EKO + Sham, EKO + CLP). Inflammatory cytokine, renal function, pyroptotic cell numbers and the levels of protein and mRNA expression of pyroptosis, including the NLRP3 (all
P
< 0.05), were analyzed and found increase in Erbin
−/−
mice with CLP and LPS-induced HK-2 cells.
Results
The inhibited of Erbin shows a renal damaged effect by promoting NLRP3 inflammasome-mediated pyroptosis in SI-AKI.
Conclusion
This study demonstrated a novel mechanism by which Erbin regulates NLRP3 inflammasome-mediated pyroptosis in SI-AKI.</description><identifier>ISSN: 1342-1751</identifier><identifier>EISSN: 1437-7799</identifier><identifier>DOI: 10.1007/s10157-023-02364-8</identifier><identifier>PMID: 37310569</identifier><language>eng</language><publisher>Singapore: Springer Nature Singapore</publisher><subject>Caspase-1 ; Cecum ; Gene expression ; Inflammasomes ; Inflammation ; Kidneys ; Lipopolysaccharides ; Medicine ; Medicine & Public Health ; Nephrology ; Original Article ; Pyroptosis ; Renal function ; Sepsis ; Urology</subject><ispartof>Clinical and experimental nephrology, 2023-09, Vol.27 (9), p.781-790</ispartof><rights>The Author(s), under exclusive licence to The Japanese Society of Nephrology 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to The Japanese Society of Nephrology.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c350t-7c35c4a6222f7fdb2114853ab3ff70ff106a24c62f37988ed31c615a2f2000b33</cites><orcidid>0000-0002-1887-8386</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10157-023-02364-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10157-023-02364-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37310569$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Yuping</creatorcontrib><creatorcontrib>Fang, Qing</creatorcontrib><creatorcontrib>Ming, Tingqian</creatorcontrib><creatorcontrib>Zuo, Jing</creatorcontrib><creatorcontrib>Jing, Guoqing</creatorcontrib><creatorcontrib>Song, Xuemin</creatorcontrib><title>Knockout of Erbin promotes pyroptosis via regulating NLRP3/caspase-1/Gasdermin D pathway in sepsis-induced acute kidney injury</title><title>Clinical and experimental nephrology</title><addtitle>Clin Exp Nephrol</addtitle><addtitle>Clin Exp Nephrol</addtitle><description>Background
This study aims to investigate the correlation between Erbin and sepsis, and the role of Erbin on the pyroptosis pathway in acute kidney injury caused by sepsis and NLRP3/caspase-1/Gasdermin D pathway.
Methods
In the study, lipopolysaccharide (LPS) treatment or cecal ligation and puncture (CLP) surgery on mice were used to stimulate the in vitro and in vivo sepsis-induced renal injury model. The male C57BL/6 of wild-type mice (WT) and Erbin-knockout mice (Erbin
−/−
, EKO) were randomly divided into four groups (WT + Sham, WT + CLP, EKO + Sham, EKO + CLP). Inflammatory cytokine, renal function, pyroptotic cell numbers and the levels of protein and mRNA expression of pyroptosis, including the NLRP3 (all
P
< 0.05), were analyzed and found increase in Erbin
−/−
mice with CLP and LPS-induced HK-2 cells.
Results
The inhibited of Erbin shows a renal damaged effect by promoting NLRP3 inflammasome-mediated pyroptosis in SI-AKI.
Conclusion
This study demonstrated a novel mechanism by which Erbin regulates NLRP3 inflammasome-mediated pyroptosis in SI-AKI.</description><subject>Caspase-1</subject><subject>Cecum</subject><subject>Gene expression</subject><subject>Inflammasomes</subject><subject>Inflammation</subject><subject>Kidneys</subject><subject>Lipopolysaccharides</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Nephrology</subject><subject>Original Article</subject><subject>Pyroptosis</subject><subject>Renal function</subject><subject>Sepsis</subject><subject>Urology</subject><issn>1342-1751</issn><issn>1437-7799</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kU9v1DAQxa2KipbCF-gBWeLCxaz_xLFzrEppK1YtQnC2HMdest3EwROD9sJnr5ctrcShB2tsze-98eghdMroB0apWgCjTCpCudiduiL6AB2zSiiiVNO8KHdRccKUZEfoFcCaUqob2bxER0IJRmXdHKM_n8fo7mKecQz4IrX9iKcUhzh7wNM2xWmO0AP-1Vuc_Cpv7NyPK3yz_PpFLJyFyYInbHFpofNpKOKPeLLzj992i8sD_FTEpB-77HyHrcuzx3d9N_pde53T9jU6DHYD_s1DPUHfP118O78iy9vL6_OzJXFC0pmoUlxla855UKFrOWOVlsK2IgRFQ2C0trxyNQ9CNVr7TjBXM2l54GXpVogT9H7vW5b7mT3MZujB-c3Gjj5mMFxzKSmvuC7ou__QdcxpLL8rVEOF0Jo2heJ7yqUIkHwwU-oHm7aGUbNLx-zTMSUZ8zcds7N--2Cd28F3j5J_cRRA7AEorXHl09PsZ2zvAaDSmqw</recordid><startdate>20230901</startdate><enddate>20230901</enddate><creator>Liu, Yuping</creator><creator>Fang, Qing</creator><creator>Ming, Tingqian</creator><creator>Zuo, Jing</creator><creator>Jing, Guoqing</creator><creator>Song, Xuemin</creator><general>Springer Nature Singapore</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1887-8386</orcidid></search><sort><creationdate>20230901</creationdate><title>Knockout of Erbin promotes pyroptosis via regulating NLRP3/caspase-1/Gasdermin D pathway in sepsis-induced acute kidney injury</title><author>Liu, Yuping ; Fang, Qing ; Ming, Tingqian ; Zuo, Jing ; Jing, Guoqing ; Song, Xuemin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c350t-7c35c4a6222f7fdb2114853ab3ff70ff106a24c62f37988ed31c615a2f2000b33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Caspase-1</topic><topic>Cecum</topic><topic>Gene expression</topic><topic>Inflammasomes</topic><topic>Inflammation</topic><topic>Kidneys</topic><topic>Lipopolysaccharides</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Nephrology</topic><topic>Original Article</topic><topic>Pyroptosis</topic><topic>Renal function</topic><topic>Sepsis</topic><topic>Urology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Yuping</creatorcontrib><creatorcontrib>Fang, Qing</creatorcontrib><creatorcontrib>Ming, Tingqian</creatorcontrib><creatorcontrib>Zuo, Jing</creatorcontrib><creatorcontrib>Jing, Guoqing</creatorcontrib><creatorcontrib>Song, Xuemin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental nephrology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Yuping</au><au>Fang, Qing</au><au>Ming, Tingqian</au><au>Zuo, Jing</au><au>Jing, Guoqing</au><au>Song, Xuemin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Knockout of Erbin promotes pyroptosis via regulating NLRP3/caspase-1/Gasdermin D pathway in sepsis-induced acute kidney injury</atitle><jtitle>Clinical and experimental nephrology</jtitle><stitle>Clin Exp Nephrol</stitle><addtitle>Clin Exp Nephrol</addtitle><date>2023-09-01</date><risdate>2023</risdate><volume>27</volume><issue>9</issue><spage>781</spage><epage>790</epage><pages>781-790</pages><issn>1342-1751</issn><eissn>1437-7799</eissn><abstract>Background
This study aims to investigate the correlation between Erbin and sepsis, and the role of Erbin on the pyroptosis pathway in acute kidney injury caused by sepsis and NLRP3/caspase-1/Gasdermin D pathway.
Methods
In the study, lipopolysaccharide (LPS) treatment or cecal ligation and puncture (CLP) surgery on mice were used to stimulate the in vitro and in vivo sepsis-induced renal injury model. The male C57BL/6 of wild-type mice (WT) and Erbin-knockout mice (Erbin
−/−
, EKO) were randomly divided into four groups (WT + Sham, WT + CLP, EKO + Sham, EKO + CLP). Inflammatory cytokine, renal function, pyroptotic cell numbers and the levels of protein and mRNA expression of pyroptosis, including the NLRP3 (all
P
< 0.05), were analyzed and found increase in Erbin
−/−
mice with CLP and LPS-induced HK-2 cells.
Results
The inhibited of Erbin shows a renal damaged effect by promoting NLRP3 inflammasome-mediated pyroptosis in SI-AKI.
Conclusion
This study demonstrated a novel mechanism by which Erbin regulates NLRP3 inflammasome-mediated pyroptosis in SI-AKI.</abstract><cop>Singapore</cop><pub>Springer Nature Singapore</pub><pmid>37310569</pmid><doi>10.1007/s10157-023-02364-8</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-1887-8386</orcidid></addata></record> |
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| subjects | Caspase-1 Cecum Gene expression Inflammasomes Inflammation Kidneys Lipopolysaccharides Medicine Medicine & Public Health Nephrology Original Article Pyroptosis Renal function Sepsis Urology |
| title | Knockout of Erbin promotes pyroptosis via regulating NLRP3/caspase-1/Gasdermin D pathway in sepsis-induced acute kidney injury |
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