Rab7/Retromer‐based endolysosomal trafficking is essential for proper host invasion in rice blast
Summary Secretion is a fundamental process that plant pathogens utilize to deliver effectors into the host to downregulate immunity and promote infection. Here, we uncover a fascinating membrane trafficking and delivery route that originates from vacuolar membranes in Magnaporthe oryzae and conduits...
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| Veröffentlicht in: | The New phytologist 2023-08, Vol.239 (4), p.1384-1403 |
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| container_title | The New phytologist |
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| creator | Chen, Xin Selvaraj, Poonguzhali Lin, Lili Fang, Wenqin Wu, Congxian Yang, Piao Zhang, Jin Abubakar, Yakubu Saddeeq Yang, Fan Lu, Guodong Liu, Wende Wang, Zonghua Naqvi, Naweed I. Zheng, Wenhui |
| description | Summary
Secretion is a fundamental process that plant pathogens utilize to deliver effectors into the host to downregulate immunity and promote infection.
Here, we uncover a fascinating membrane trafficking and delivery route that originates from vacuolar membranes in Magnaporthe oryzae and conduits to the host interface and plasma membrane.
To perform such secretory/trafficking function, MoRab7 first recruits the retromer complex to the vacuolar membrane, enabling recognition of a family of SNARE proteins, including MoSnc1. Live‐cell imaging confirmed a highly dynamic vesicular trafficking of the retromer complex component(s) and MoSnc1 toward and across the host interface or plasma membrane, and subsequent fusion with target membranes. Interestingly, disruption of the MoRab7/Retromer/MoSnc1‐based endolysosomal cascade affects effector secretion and fungal pathogenicity.
Taken together, we discovered an unconventional protein and membrane trafficking route starting from the fungal endolysosomes to the M. oryzae–rice interaction interface and dissect the role of MoRab7/Retromer/MoSnc1 sorting machinery in effector secretion during biotrophy and invasive growth in rice blast fungus. |
| doi_str_mv | 10.1111/nph.19050 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2824686409</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2824686409</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3860-f81e1496af174c4cd463964e0435e5bcd8926c5b20ca922c998b1fb8f6709fa73</originalsourceid><addsrcrecordid>eNqN0cFKHTEUBuBQKvVqu-gLSKAbXYw3yWQyyVIutgqiRVrobshkTmrszOSaM1e5Ox-hz9gnMXqti4LQbBLIx885_IR85OyQ5zMfl1eH3LCKvSEzLpUpNC_rt2TGmNCFkurHNtlBvGaMmUqJd2S7rIXh2lQz4i5tW88vYUpxgPTn_ndrEToKYxf7NUaMg-3plKz3wf0K408akAIijFPIHz4mukxxCYleRZxoGG8thjjmB03BAW17i9N7suVtj_Dh-d4l3z8ff1ucFGcXX04XR2eFK7VihdccuDTKel5LJ10nVWmUBCbLCqrWddoI5apWMGeNEM4Y3XLfaq9qZryty12yv8nNI92sAKdmCOig7-0IcYWN0NJoU2ph_oMKqbSS7JF--odex1Ua8yJZlYoLWdc8q4ONcikiJvDNMoXBpnXDWfNYUpNLap5KynbvOXHVDtC9yL-tZDDfgLvQw_r1pOb868km8gGfgJwZ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2836124771</pqid></control><display><type>article</type><title>Rab7/Retromer‐based endolysosomal trafficking is essential for proper host invasion in rice blast</title><source>Wiley Free Content</source><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Chen, Xin ; Selvaraj, Poonguzhali ; Lin, Lili ; Fang, Wenqin ; Wu, Congxian ; Yang, Piao ; Zhang, Jin ; Abubakar, Yakubu Saddeeq ; Yang, Fan ; Lu, Guodong ; Liu, Wende ; Wang, Zonghua ; Naqvi, Naweed I. ; Zheng, Wenhui</creator><creatorcontrib>Chen, Xin ; Selvaraj, Poonguzhali ; Lin, Lili ; Fang, Wenqin ; Wu, Congxian ; Yang, Piao ; Zhang, Jin ; Abubakar, Yakubu Saddeeq ; Yang, Fan ; Lu, Guodong ; Liu, Wende ; Wang, Zonghua ; Naqvi, Naweed I. ; Zheng, Wenhui</creatorcontrib><description>Summary
Secretion is a fundamental process that plant pathogens utilize to deliver effectors into the host to downregulate immunity and promote infection.
Here, we uncover a fascinating membrane trafficking and delivery route that originates from vacuolar membranes in Magnaporthe oryzae and conduits to the host interface and plasma membrane.
To perform such secretory/trafficking function, MoRab7 first recruits the retromer complex to the vacuolar membrane, enabling recognition of a family of SNARE proteins, including MoSnc1. Live‐cell imaging confirmed a highly dynamic vesicular trafficking of the retromer complex component(s) and MoSnc1 toward and across the host interface or plasma membrane, and subsequent fusion with target membranes. Interestingly, disruption of the MoRab7/Retromer/MoSnc1‐based endolysosomal cascade affects effector secretion and fungal pathogenicity.
Taken together, we discovered an unconventional protein and membrane trafficking route starting from the fungal endolysosomes to the M. oryzae–rice interaction interface and dissect the role of MoRab7/Retromer/MoSnc1 sorting machinery in effector secretion during biotrophy and invasive growth in rice blast fungus.</description><identifier>ISSN: 0028-646X</identifier><identifier>EISSN: 1469-8137</identifier><identifier>DOI: 10.1111/nph.19050</identifier><identifier>PMID: 37291895</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Biological Transport ; blast disease ; Cell Membrane - metabolism ; effector secretion ; endolysosomal trafficking ; Endosomes - metabolism ; Fungal Proteins - metabolism ; Fungi ; Immunity ; Magnaporthe ; Magnaporthe oryzae ; Membrane fusion ; Membrane proteins ; Membrane trafficking ; Membranes ; Oryza - metabolism ; Pathogenicity ; Pathogens ; Plant Diseases - microbiology ; plasma membrane ; Protein Transport ; Proteins ; Rab GTPase MoRab7 ; retromer ; Rice blast ; Secretion ; SNAP receptors ; SNARE ; vacuoles ; Vacuoles - metabolism</subject><ispartof>The New phytologist, 2023-08, Vol.239 (4), p.1384-1403</ispartof><rights>2023 The Authors. © 2023 New Phytologist Foundation</rights><rights>2023 The Authors. New Phytologist © 2023 New Phytologist Foundation.</rights><rights>Copyright © 2023 New Phytologist Trust</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3860-f81e1496af174c4cd463964e0435e5bcd8926c5b20ca922c998b1fb8f6709fa73</citedby><cites>FETCH-LOGICAL-c3860-f81e1496af174c4cd463964e0435e5bcd8926c5b20ca922c998b1fb8f6709fa73</cites><orcidid>0000-0002-0869-9683 ; 0000-0003-1506-9337 ; 0000-0002-3619-4906 ; 0000-0002-0208-2375 ; 0000-0002-0761-6233</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fnph.19050$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fnph.19050$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37291895$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Xin</creatorcontrib><creatorcontrib>Selvaraj, Poonguzhali</creatorcontrib><creatorcontrib>Lin, Lili</creatorcontrib><creatorcontrib>Fang, Wenqin</creatorcontrib><creatorcontrib>Wu, Congxian</creatorcontrib><creatorcontrib>Yang, Piao</creatorcontrib><creatorcontrib>Zhang, Jin</creatorcontrib><creatorcontrib>Abubakar, Yakubu Saddeeq</creatorcontrib><creatorcontrib>Yang, Fan</creatorcontrib><creatorcontrib>Lu, Guodong</creatorcontrib><creatorcontrib>Liu, Wende</creatorcontrib><creatorcontrib>Wang, Zonghua</creatorcontrib><creatorcontrib>Naqvi, Naweed I.</creatorcontrib><creatorcontrib>Zheng, Wenhui</creatorcontrib><title>Rab7/Retromer‐based endolysosomal trafficking is essential for proper host invasion in rice blast</title><title>The New phytologist</title><addtitle>New Phytol</addtitle><description>Summary
Secretion is a fundamental process that plant pathogens utilize to deliver effectors into the host to downregulate immunity and promote infection.
Here, we uncover a fascinating membrane trafficking and delivery route that originates from vacuolar membranes in Magnaporthe oryzae and conduits to the host interface and plasma membrane.
To perform such secretory/trafficking function, MoRab7 first recruits the retromer complex to the vacuolar membrane, enabling recognition of a family of SNARE proteins, including MoSnc1. Live‐cell imaging confirmed a highly dynamic vesicular trafficking of the retromer complex component(s) and MoSnc1 toward and across the host interface or plasma membrane, and subsequent fusion with target membranes. Interestingly, disruption of the MoRab7/Retromer/MoSnc1‐based endolysosomal cascade affects effector secretion and fungal pathogenicity.
Taken together, we discovered an unconventional protein and membrane trafficking route starting from the fungal endolysosomes to the M. oryzae–rice interaction interface and dissect the role of MoRab7/Retromer/MoSnc1 sorting machinery in effector secretion during biotrophy and invasive growth in rice blast fungus.</description><subject>Biological Transport</subject><subject>blast disease</subject><subject>Cell Membrane - metabolism</subject><subject>effector secretion</subject><subject>endolysosomal trafficking</subject><subject>Endosomes - metabolism</subject><subject>Fungal Proteins - metabolism</subject><subject>Fungi</subject><subject>Immunity</subject><subject>Magnaporthe</subject><subject>Magnaporthe oryzae</subject><subject>Membrane fusion</subject><subject>Membrane proteins</subject><subject>Membrane trafficking</subject><subject>Membranes</subject><subject>Oryza - metabolism</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Plant Diseases - microbiology</subject><subject>plasma membrane</subject><subject>Protein Transport</subject><subject>Proteins</subject><subject>Rab GTPase MoRab7</subject><subject>retromer</subject><subject>Rice blast</subject><subject>Secretion</subject><subject>SNAP receptors</subject><subject>SNARE</subject><subject>vacuoles</subject><subject>Vacuoles - metabolism</subject><issn>0028-646X</issn><issn>1469-8137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0cFKHTEUBuBQKvVqu-gLSKAbXYw3yWQyyVIutgqiRVrobshkTmrszOSaM1e5Ox-hz9gnMXqti4LQbBLIx885_IR85OyQ5zMfl1eH3LCKvSEzLpUpNC_rt2TGmNCFkurHNtlBvGaMmUqJd2S7rIXh2lQz4i5tW88vYUpxgPTn_ndrEToKYxf7NUaMg-3plKz3wf0K408akAIijFPIHz4mukxxCYleRZxoGG8thjjmB03BAW17i9N7suVtj_Dh-d4l3z8ff1ucFGcXX04XR2eFK7VihdccuDTKel5LJ10nVWmUBCbLCqrWddoI5apWMGeNEM4Y3XLfaq9qZryty12yv8nNI92sAKdmCOig7-0IcYWN0NJoU2ph_oMKqbSS7JF--odex1Ua8yJZlYoLWdc8q4ONcikiJvDNMoXBpnXDWfNYUpNLap5KynbvOXHVDtC9yL-tZDDfgLvQw_r1pOb868km8gGfgJwZ</recordid><startdate>202308</startdate><enddate>202308</enddate><creator>Chen, Xin</creator><creator>Selvaraj, Poonguzhali</creator><creator>Lin, Lili</creator><creator>Fang, Wenqin</creator><creator>Wu, Congxian</creator><creator>Yang, Piao</creator><creator>Zhang, Jin</creator><creator>Abubakar, Yakubu Saddeeq</creator><creator>Yang, Fan</creator><creator>Lu, Guodong</creator><creator>Liu, Wende</creator><creator>Wang, Zonghua</creator><creator>Naqvi, Naweed I.</creator><creator>Zheng, Wenhui</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-0869-9683</orcidid><orcidid>https://orcid.org/0000-0003-1506-9337</orcidid><orcidid>https://orcid.org/0000-0002-3619-4906</orcidid><orcidid>https://orcid.org/0000-0002-0208-2375</orcidid><orcidid>https://orcid.org/0000-0002-0761-6233</orcidid></search><sort><creationdate>202308</creationdate><title>Rab7/Retromer‐based endolysosomal trafficking is essential for proper host invasion in rice blast</title><author>Chen, Xin ; Selvaraj, Poonguzhali ; Lin, Lili ; Fang, Wenqin ; Wu, Congxian ; Yang, Piao ; Zhang, Jin ; Abubakar, Yakubu Saddeeq ; Yang, Fan ; Lu, Guodong ; Liu, Wende ; Wang, Zonghua ; Naqvi, Naweed I. ; Zheng, Wenhui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3860-f81e1496af174c4cd463964e0435e5bcd8926c5b20ca922c998b1fb8f6709fa73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Biological Transport</topic><topic>blast disease</topic><topic>Cell Membrane - metabolism</topic><topic>effector secretion</topic><topic>endolysosomal trafficking</topic><topic>Endosomes - metabolism</topic><topic>Fungal Proteins - metabolism</topic><topic>Fungi</topic><topic>Immunity</topic><topic>Magnaporthe</topic><topic>Magnaporthe oryzae</topic><topic>Membrane fusion</topic><topic>Membrane proteins</topic><topic>Membrane trafficking</topic><topic>Membranes</topic><topic>Oryza - metabolism</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Plant Diseases - microbiology</topic><topic>plasma membrane</topic><topic>Protein Transport</topic><topic>Proteins</topic><topic>Rab GTPase MoRab7</topic><topic>retromer</topic><topic>Rice blast</topic><topic>Secretion</topic><topic>SNAP receptors</topic><topic>SNARE</topic><topic>vacuoles</topic><topic>Vacuoles - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Xin</creatorcontrib><creatorcontrib>Selvaraj, Poonguzhali</creatorcontrib><creatorcontrib>Lin, Lili</creatorcontrib><creatorcontrib>Fang, Wenqin</creatorcontrib><creatorcontrib>Wu, Congxian</creatorcontrib><creatorcontrib>Yang, Piao</creatorcontrib><creatorcontrib>Zhang, Jin</creatorcontrib><creatorcontrib>Abubakar, Yakubu Saddeeq</creatorcontrib><creatorcontrib>Yang, Fan</creatorcontrib><creatorcontrib>Lu, Guodong</creatorcontrib><creatorcontrib>Liu, Wende</creatorcontrib><creatorcontrib>Wang, Zonghua</creatorcontrib><creatorcontrib>Naqvi, Naweed I.</creatorcontrib><creatorcontrib>Zheng, Wenhui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>The New phytologist</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Xin</au><au>Selvaraj, Poonguzhali</au><au>Lin, Lili</au><au>Fang, Wenqin</au><au>Wu, Congxian</au><au>Yang, Piao</au><au>Zhang, Jin</au><au>Abubakar, Yakubu Saddeeq</au><au>Yang, Fan</au><au>Lu, Guodong</au><au>Liu, Wende</au><au>Wang, Zonghua</au><au>Naqvi, Naweed I.</au><au>Zheng, Wenhui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rab7/Retromer‐based endolysosomal trafficking is essential for proper host invasion in rice blast</atitle><jtitle>The New phytologist</jtitle><addtitle>New Phytol</addtitle><date>2023-08</date><risdate>2023</risdate><volume>239</volume><issue>4</issue><spage>1384</spage><epage>1403</epage><pages>1384-1403</pages><issn>0028-646X</issn><eissn>1469-8137</eissn><abstract>Summary
Secretion is a fundamental process that plant pathogens utilize to deliver effectors into the host to downregulate immunity and promote infection.
Here, we uncover a fascinating membrane trafficking and delivery route that originates from vacuolar membranes in Magnaporthe oryzae and conduits to the host interface and plasma membrane.
To perform such secretory/trafficking function, MoRab7 first recruits the retromer complex to the vacuolar membrane, enabling recognition of a family of SNARE proteins, including MoSnc1. Live‐cell imaging confirmed a highly dynamic vesicular trafficking of the retromer complex component(s) and MoSnc1 toward and across the host interface or plasma membrane, and subsequent fusion with target membranes. Interestingly, disruption of the MoRab7/Retromer/MoSnc1‐based endolysosomal cascade affects effector secretion and fungal pathogenicity.
Taken together, we discovered an unconventional protein and membrane trafficking route starting from the fungal endolysosomes to the M. oryzae–rice interaction interface and dissect the role of MoRab7/Retromer/MoSnc1 sorting machinery in effector secretion during biotrophy and invasive growth in rice blast fungus.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>37291895</pmid><doi>10.1111/nph.19050</doi><tpages>1403</tpages><orcidid>https://orcid.org/0000-0002-0869-9683</orcidid><orcidid>https://orcid.org/0000-0003-1506-9337</orcidid><orcidid>https://orcid.org/0000-0002-3619-4906</orcidid><orcidid>https://orcid.org/0000-0002-0208-2375</orcidid><orcidid>https://orcid.org/0000-0002-0761-6233</orcidid></addata></record> |
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| subjects | Biological Transport blast disease Cell Membrane - metabolism effector secretion endolysosomal trafficking Endosomes - metabolism Fungal Proteins - metabolism Fungi Immunity Magnaporthe Magnaporthe oryzae Membrane fusion Membrane proteins Membrane trafficking Membranes Oryza - metabolism Pathogenicity Pathogens Plant Diseases - microbiology plasma membrane Protein Transport Proteins Rab GTPase MoRab7 retromer Rice blast Secretion SNAP receptors SNARE vacuoles Vacuoles - metabolism |
| title | Rab7/Retromer‐based endolysosomal trafficking is essential for proper host invasion in rice blast |
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