Liquid storage of porcine in vitro -produced blastocysts; a practical approach for short storage
Commercial application of embryo transfer in pig breeding is dependent on the storage of embryos. The aim of this study was to assess the embryo quality of -produced blastocysts after 3 h liquid storage at 37°C in CO -free medium by evaluating morphology, developmental capacity and apoptosis. Blasto...
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| Veröffentlicht in: | Zygote (Cambridge) 2023-10, Vol.31 (5), p.1-450 |
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| creator | Haug, Linda Marijke Jochems, Reina Gaustad, Ann Helen Kommisrud, Elisabeth Myromslien, Frøydis Deinboll Grindflek, Eli Alm-Kristiansen, Anne Hege |
| description | Commercial application of embryo transfer in pig breeding is dependent on the storage of embryos. The aim of this study was to assess the embryo quality of
-produced blastocysts after 3 h liquid storage at 37°C in CO
-free medium by evaluating morphology,
developmental capacity and apoptosis. Blastocysts at days 5 and 6 post-fertilization were randomly allocated to the storage group (HEPES-buffered NCSU-23 medium including bovine serum albumin in a portable embryo transport incubator at 37°C) or a control group (porcine blastocyst medium in a conventional culture incubator). Thereafter, blastocysts were evaluated for morphology and stained to assess apoptosis straight after the 3 h storage period or after a further 24 h conventional incubation. There was no significant difference between the storage and control group after 3 h storage and the further 24 h conventional incubation for any of the parameters, nor for apoptosis straight after the 3 h storage. Embryos that reached the blastocyst stage at day 5 showed less apoptosis (6.6% vs 10.9%,
= 0.01) and a trend for a higher rate of developmental capacity (70.6% vs 51.5%,
= 0.089) than embryos reaching the blastocyst stage on day 6. In conclusion,
-produced porcine blastocysts can be stored for 3 h at physiological temperature in transportable incubators using a CO
-independent medium without compromising quality. |
| doi_str_mv | 10.1017/S0967199423000308 |
| format | Article |
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-produced blastocysts after 3 h liquid storage at 37°C in CO
-free medium by evaluating morphology,
developmental capacity and apoptosis. Blastocysts at days 5 and 6 post-fertilization were randomly allocated to the storage group (HEPES-buffered NCSU-23 medium including bovine serum albumin in a portable embryo transport incubator at 37°C) or a control group (porcine blastocyst medium in a conventional culture incubator). Thereafter, blastocysts were evaluated for morphology and stained to assess apoptosis straight after the 3 h storage period or after a further 24 h conventional incubation. There was no significant difference between the storage and control group after 3 h storage and the further 24 h conventional incubation for any of the parameters, nor for apoptosis straight after the 3 h storage. Embryos that reached the blastocyst stage at day 5 showed less apoptosis (6.6% vs 10.9%,
= 0.01) and a trend for a higher rate of developmental capacity (70.6% vs 51.5%,
= 0.089) than embryos reaching the blastocyst stage on day 6. In conclusion,
-produced porcine blastocysts can be stored for 3 h at physiological temperature in transportable incubators using a CO
-independent medium without compromising quality.</description><identifier>ISSN: 0967-1994</identifier><identifier>EISSN: 1469-8730</identifier><identifier>DOI: 10.1017/S0967199423000308</identifier><identifier>PMID: 37288532</identifier><language>eng</language><publisher>England: Cambridge University Press</publisher><subject>Amino acids ; Animal husbandry ; Animal welfare ; Apoptosis ; Blastocysts ; Bovine serum albumin ; Carbon dioxide ; Cryopreservation ; Embryo transfer ; Embryos ; Fertilization ; Hogs ; In vitro fertilization ; Incubation ; Incubators ; Lipids ; Morphology ; Ovaries ; Pathogens ; Polyvinyl alcohol ; Quality assessment ; Serum albumin ; Sperm ; Storage</subject><ispartof>Zygote (Cambridge), 2023-10, Vol.31 (5), p.1-450</ispartof><rights>The Author(s), 2023. Published by Cambridge University Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c329t-e0d1d356d53d4bcd66179bea51490708887aa0d4f58f68f9e6261dafb175ca6b3</citedby><cites>FETCH-LOGICAL-c329t-e0d1d356d53d4bcd66179bea51490708887aa0d4f58f68f9e6261dafb175ca6b3</cites><orcidid>0000-0003-4350-1887 ; 0000-0002-9991-3496</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37288532$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Haug, Linda Marijke</creatorcontrib><creatorcontrib>Jochems, Reina</creatorcontrib><creatorcontrib>Gaustad, Ann Helen</creatorcontrib><creatorcontrib>Kommisrud, Elisabeth</creatorcontrib><creatorcontrib>Myromslien, Frøydis Deinboll</creatorcontrib><creatorcontrib>Grindflek, Eli</creatorcontrib><creatorcontrib>Alm-Kristiansen, Anne Hege</creatorcontrib><title>Liquid storage of porcine in vitro -produced blastocysts; a practical approach for short storage</title><title>Zygote (Cambridge)</title><addtitle>Zygote</addtitle><description>Commercial application of embryo transfer in pig breeding is dependent on the storage of embryos. The aim of this study was to assess the embryo quality of
-produced blastocysts after 3 h liquid storage at 37°C in CO
-free medium by evaluating morphology,
developmental capacity and apoptosis. Blastocysts at days 5 and 6 post-fertilization were randomly allocated to the storage group (HEPES-buffered NCSU-23 medium including bovine serum albumin in a portable embryo transport incubator at 37°C) or a control group (porcine blastocyst medium in a conventional culture incubator). Thereafter, blastocysts were evaluated for morphology and stained to assess apoptosis straight after the 3 h storage period or after a further 24 h conventional incubation. There was no significant difference between the storage and control group after 3 h storage and the further 24 h conventional incubation for any of the parameters, nor for apoptosis straight after the 3 h storage. Embryos that reached the blastocyst stage at day 5 showed less apoptosis (6.6% vs 10.9%,
= 0.01) and a trend for a higher rate of developmental capacity (70.6% vs 51.5%,
= 0.089) than embryos reaching the blastocyst stage on day 6. In conclusion,
-produced porcine blastocysts can be stored for 3 h at physiological temperature in transportable incubators using a CO
-independent medium without compromising quality.</description><subject>Amino acids</subject><subject>Animal husbandry</subject><subject>Animal welfare</subject><subject>Apoptosis</subject><subject>Blastocysts</subject><subject>Bovine serum albumin</subject><subject>Carbon dioxide</subject><subject>Cryopreservation</subject><subject>Embryo transfer</subject><subject>Embryos</subject><subject>Fertilization</subject><subject>Hogs</subject><subject>In vitro fertilization</subject><subject>Incubation</subject><subject>Incubators</subject><subject>Lipids</subject><subject>Morphology</subject><subject>Ovaries</subject><subject>Pathogens</subject><subject>Polyvinyl alcohol</subject><subject>Quality assessment</subject><subject>Serum albumin</subject><subject>Sperm</subject><subject>Storage</subject><issn>0967-1994</issn><issn>1469-8730</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpl0MtKAzEUBuAgiq3VB3AjATduRnObXHAlxRsUXKjrMZOLnTJtxmRG6Nub0taFrs7ifOfn8ANwjtE1RljcvCLFBVaKEYoQokgegDFmXBVSUHQIxpt1sdmPwElKi2yEUOwYjKggUpaUjMHHrPkaGgtTH6L-dDB42IVompWDzQp-N30MsOhisINxFtatztCsU59uoYZd1KZvjG6h7rLRZg59iDDNQ-z3iafgyOs2ubPdnID3h_u36VMxe3l8nt7NCkOJ6guHLLa05LakltXGco6Fqp0uMVNIICml0BpZ5kvpufTKccKx1b7GojSa13QCrra5-ZGvwaW-WjbJuLbVKxeGVBFJqJKSlCzTyz90EYa4yt9lpQglSDCeFd4qE0NK0fmqi81Sx3WFUbWpv_pXf7652CUP9dLZ34t93_QHTAt_2Q</recordid><startdate>20231001</startdate><enddate>20231001</enddate><creator>Haug, Linda Marijke</creator><creator>Jochems, Reina</creator><creator>Gaustad, Ann Helen</creator><creator>Kommisrud, Elisabeth</creator><creator>Myromslien, Frøydis Deinboll</creator><creator>Grindflek, Eli</creator><creator>Alm-Kristiansen, Anne Hege</creator><general>Cambridge University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4350-1887</orcidid><orcidid>https://orcid.org/0000-0002-9991-3496</orcidid></search><sort><creationdate>20231001</creationdate><title>Liquid storage of porcine in vitro -produced blastocysts; a practical approach for short storage</title><author>Haug, Linda Marijke ; Jochems, Reina ; Gaustad, Ann Helen ; Kommisrud, Elisabeth ; Myromslien, Frøydis Deinboll ; Grindflek, Eli ; Alm-Kristiansen, Anne Hege</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c329t-e0d1d356d53d4bcd66179bea51490708887aa0d4f58f68f9e6261dafb175ca6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Amino acids</topic><topic>Animal husbandry</topic><topic>Animal welfare</topic><topic>Apoptosis</topic><topic>Blastocysts</topic><topic>Bovine serum albumin</topic><topic>Carbon dioxide</topic><topic>Cryopreservation</topic><topic>Embryo transfer</topic><topic>Embryos</topic><topic>Fertilization</topic><topic>Hogs</topic><topic>In vitro fertilization</topic><topic>Incubation</topic><topic>Incubators</topic><topic>Lipids</topic><topic>Morphology</topic><topic>Ovaries</topic><topic>Pathogens</topic><topic>Polyvinyl alcohol</topic><topic>Quality assessment</topic><topic>Serum albumin</topic><topic>Sperm</topic><topic>Storage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Haug, Linda Marijke</creatorcontrib><creatorcontrib>Jochems, Reina</creatorcontrib><creatorcontrib>Gaustad, Ann Helen</creatorcontrib><creatorcontrib>Kommisrud, Elisabeth</creatorcontrib><creatorcontrib>Myromslien, Frøydis Deinboll</creatorcontrib><creatorcontrib>Grindflek, Eli</creatorcontrib><creatorcontrib>Alm-Kristiansen, Anne Hege</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Zygote (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haug, Linda Marijke</au><au>Jochems, Reina</au><au>Gaustad, Ann Helen</au><au>Kommisrud, Elisabeth</au><au>Myromslien, Frøydis Deinboll</au><au>Grindflek, Eli</au><au>Alm-Kristiansen, Anne Hege</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Liquid storage of porcine in vitro -produced blastocysts; a practical approach for short storage</atitle><jtitle>Zygote (Cambridge)</jtitle><addtitle>Zygote</addtitle><date>2023-10-01</date><risdate>2023</risdate><volume>31</volume><issue>5</issue><spage>1</spage><epage>450</epage><pages>1-450</pages><issn>0967-1994</issn><eissn>1469-8730</eissn><abstract>Commercial application of embryo transfer in pig breeding is dependent on the storage of embryos. The aim of this study was to assess the embryo quality of
-produced blastocysts after 3 h liquid storage at 37°C in CO
-free medium by evaluating morphology,
developmental capacity and apoptosis. Blastocysts at days 5 and 6 post-fertilization were randomly allocated to the storage group (HEPES-buffered NCSU-23 medium including bovine serum albumin in a portable embryo transport incubator at 37°C) or a control group (porcine blastocyst medium in a conventional culture incubator). Thereafter, blastocysts were evaluated for morphology and stained to assess apoptosis straight after the 3 h storage period or after a further 24 h conventional incubation. There was no significant difference between the storage and control group after 3 h storage and the further 24 h conventional incubation for any of the parameters, nor for apoptosis straight after the 3 h storage. Embryos that reached the blastocyst stage at day 5 showed less apoptosis (6.6% vs 10.9%,
= 0.01) and a trend for a higher rate of developmental capacity (70.6% vs 51.5%,
= 0.089) than embryos reaching the blastocyst stage on day 6. In conclusion,
-produced porcine blastocysts can be stored for 3 h at physiological temperature in transportable incubators using a CO
-independent medium without compromising quality.</abstract><cop>England</cop><pub>Cambridge University Press</pub><pmid>37288532</pmid><doi>10.1017/S0967199423000308</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-4350-1887</orcidid><orcidid>https://orcid.org/0000-0002-9991-3496</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0967-1994 |
| ispartof | Zygote (Cambridge), 2023-10, Vol.31 (5), p.1-450 |
| issn | 0967-1994 1469-8730 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2823988254 |
| source | Cambridge University Press Journals Complete |
| subjects | Amino acids Animal husbandry Animal welfare Apoptosis Blastocysts Bovine serum albumin Carbon dioxide Cryopreservation Embryo transfer Embryos Fertilization Hogs In vitro fertilization Incubation Incubators Lipids Morphology Ovaries Pathogens Polyvinyl alcohol Quality assessment Serum albumin Sperm Storage |
| title | Liquid storage of porcine in vitro -produced blastocysts; a practical approach for short storage |
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