Inhibition of suplatast tosilate on EPSC in the single paratracheal ganglion neurons attached with presynaptic boutons
Using single neurons of rat paratracheal ganglia (PTG) attached with presynaptic boutons, the effects of suplatast tosilate on excitatory postsynaptic currents (EPSCs) were investigated with nystatin-perforated patch-clamp recording technique. We found that suplatast concentration dependently inhibi...
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| Veröffentlicht in: | Journal of neurophysiology 2023-07, Vol.130 (1), p.56-60 |
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| creator | Zhou, Jian-Rong Shirasaki, Tetsuya Soeda, Fumio Takahama, Kazuo |
| description | Using single neurons of rat paratracheal ganglia (PTG) attached with presynaptic boutons, the effects of suplatast tosilate on excitatory postsynaptic currents (EPSCs) were investigated with nystatin-perforated patch-clamp recording technique. We found that suplatast concentration dependently inhibited the EPSC amplitude and its frequency in single PTG neurons attached with presynaptic boutons. EPSC frequency was higher sensitive to suplatast than EPSC amplitude. IC
for EPSC frequency was 1.1 × 10
M, being similar to that for the effect on histamine release from mast cells and lower than that for the inhibitory effect on cytokine production. Suplatast also inhibited the EPSCs potentiated by bradykinin (BK), but it did not affect the potentiation itself by BK. Thus suplatast inhibited the EPSC of PTG neurons attached with presynaptic boutons at both the presynaptic and postsynaptic sites.
In this study, using single neurons of rat paratracheal ganglia (PTG) attached with presynaptic boutons, the effects of suplatast tosilate on excitatory postsynaptic currents (EPSCs) were investigated with patch-clamp recording technique. We found that suplatast concentration dependently inhibited the EPSC amplitude and its frequency in single PTG neurons attached with presynaptic boutons. Thus suplatast inhibited the function of PTG neurons at both of presynaptic and postsynaptic sites. |
| doi_str_mv | 10.1152/jn.00151.2023 |
| format | Article |
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for EPSC frequency was 1.1 × 10
M, being similar to that for the effect on histamine release from mast cells and lower than that for the inhibitory effect on cytokine production. Suplatast also inhibited the EPSCs potentiated by bradykinin (BK), but it did not affect the potentiation itself by BK. Thus suplatast inhibited the EPSC of PTG neurons attached with presynaptic boutons at both the presynaptic and postsynaptic sites.
In this study, using single neurons of rat paratracheal ganglia (PTG) attached with presynaptic boutons, the effects of suplatast tosilate on excitatory postsynaptic currents (EPSCs) were investigated with patch-clamp recording technique. We found that suplatast concentration dependently inhibited the EPSC amplitude and its frequency in single PTG neurons attached with presynaptic boutons. Thus suplatast inhibited the function of PTG neurons at both of presynaptic and postsynaptic sites.</description><identifier>ISSN: 0022-3077</identifier><identifier>EISSN: 1522-1598</identifier><identifier>DOI: 10.1152/jn.00151.2023</identifier><identifier>PMID: 37283483</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Arylsulfonates - pharmacology ; Bradykinin - pharmacology ; Ganglia ; Neurons - physiology ; Rats ; Sulfonium Compounds - pharmacology</subject><ispartof>Journal of neurophysiology, 2023-07, Vol.130 (1), p.56-60</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c249t-44ccf987b87d56f6d1041bb0a3c02b09bdd433e7b3157575b630a1bf5c0e9823</cites><orcidid>0000-0002-7638-7577 ; 0000-0002-9402-2838</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3026,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37283483$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Jian-Rong</creatorcontrib><creatorcontrib>Shirasaki, Tetsuya</creatorcontrib><creatorcontrib>Soeda, Fumio</creatorcontrib><creatorcontrib>Takahama, Kazuo</creatorcontrib><title>Inhibition of suplatast tosilate on EPSC in the single paratracheal ganglion neurons attached with presynaptic boutons</title><title>Journal of neurophysiology</title><addtitle>J Neurophysiol</addtitle><description>Using single neurons of rat paratracheal ganglia (PTG) attached with presynaptic boutons, the effects of suplatast tosilate on excitatory postsynaptic currents (EPSCs) were investigated with nystatin-perforated patch-clamp recording technique. We found that suplatast concentration dependently inhibited the EPSC amplitude and its frequency in single PTG neurons attached with presynaptic boutons. EPSC frequency was higher sensitive to suplatast than EPSC amplitude. IC
for EPSC frequency was 1.1 × 10
M, being similar to that for the effect on histamine release from mast cells and lower than that for the inhibitory effect on cytokine production. Suplatast also inhibited the EPSCs potentiated by bradykinin (BK), but it did not affect the potentiation itself by BK. Thus suplatast inhibited the EPSC of PTG neurons attached with presynaptic boutons at both the presynaptic and postsynaptic sites.
In this study, using single neurons of rat paratracheal ganglia (PTG) attached with presynaptic boutons, the effects of suplatast tosilate on excitatory postsynaptic currents (EPSCs) were investigated with patch-clamp recording technique. We found that suplatast concentration dependently inhibited the EPSC amplitude and its frequency in single PTG neurons attached with presynaptic boutons. Thus suplatast inhibited the function of PTG neurons at both of presynaptic and postsynaptic sites.</description><subject>Animals</subject><subject>Arylsulfonates - pharmacology</subject><subject>Bradykinin - pharmacology</subject><subject>Ganglia</subject><subject>Neurons - physiology</subject><subject>Rats</subject><subject>Sulfonium Compounds - pharmacology</subject><issn>0022-3077</issn><issn>1522-1598</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kDFPwzAUhC0EoqUwsiKPLCnPdlwnI6oKVKoEEt0j23FaV6kTbAfUf48LBb3hne4-3XAI3RKYEsLpw85NAQgnUwqUnaFx8mhGeFmcozFA0gyEGKGrEHYAIDjQSzRighYsL9gYfS7d1iobbedw1-Aw9K2MMkQcu2CTNDgFi7f3ObYOx63BwbpNa3AvvYxe6q2RLd7I5B0bnBl85wKWMR6jGn_ZuMW9N-HgZB-txqobYiKu0UUj22BuTn-C1k-L9fwlW70-L-ePq0zTvIxZnmvdlIVQhaj5rJnVBHKiFEimgSooVV3njBmhGOEinZoxkEQ1XIMpC8om6P63tvfdx2BCrPY2aNO20pluCBVNDAcieJHQ7BfVvgvBm6bqvd1Lf6gIVMelq52rfpaujksn_u5UPai9qf_pv2nZN6EeewU</recordid><startdate>20230701</startdate><enddate>20230701</enddate><creator>Zhou, Jian-Rong</creator><creator>Shirasaki, Tetsuya</creator><creator>Soeda, Fumio</creator><creator>Takahama, Kazuo</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-7638-7577</orcidid><orcidid>https://orcid.org/0000-0002-9402-2838</orcidid></search><sort><creationdate>20230701</creationdate><title>Inhibition of suplatast tosilate on EPSC in the single paratracheal ganglion neurons attached with presynaptic boutons</title><author>Zhou, Jian-Rong ; Shirasaki, Tetsuya ; Soeda, Fumio ; Takahama, Kazuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c249t-44ccf987b87d56f6d1041bb0a3c02b09bdd433e7b3157575b630a1bf5c0e9823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Arylsulfonates - pharmacology</topic><topic>Bradykinin - pharmacology</topic><topic>Ganglia</topic><topic>Neurons - physiology</topic><topic>Rats</topic><topic>Sulfonium Compounds - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Jian-Rong</creatorcontrib><creatorcontrib>Shirasaki, Tetsuya</creatorcontrib><creatorcontrib>Soeda, Fumio</creatorcontrib><creatorcontrib>Takahama, Kazuo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurophysiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Jian-Rong</au><au>Shirasaki, Tetsuya</au><au>Soeda, Fumio</au><au>Takahama, Kazuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of suplatast tosilate on EPSC in the single paratracheal ganglion neurons attached with presynaptic boutons</atitle><jtitle>Journal of neurophysiology</jtitle><addtitle>J Neurophysiol</addtitle><date>2023-07-01</date><risdate>2023</risdate><volume>130</volume><issue>1</issue><spage>56</spage><epage>60</epage><pages>56-60</pages><issn>0022-3077</issn><eissn>1522-1598</eissn><abstract>Using single neurons of rat paratracheal ganglia (PTG) attached with presynaptic boutons, the effects of suplatast tosilate on excitatory postsynaptic currents (EPSCs) were investigated with nystatin-perforated patch-clamp recording technique. We found that suplatast concentration dependently inhibited the EPSC amplitude and its frequency in single PTG neurons attached with presynaptic boutons. EPSC frequency was higher sensitive to suplatast than EPSC amplitude. IC
for EPSC frequency was 1.1 × 10
M, being similar to that for the effect on histamine release from mast cells and lower than that for the inhibitory effect on cytokine production. Suplatast also inhibited the EPSCs potentiated by bradykinin (BK), but it did not affect the potentiation itself by BK. Thus suplatast inhibited the EPSC of PTG neurons attached with presynaptic boutons at both the presynaptic and postsynaptic sites.
In this study, using single neurons of rat paratracheal ganglia (PTG) attached with presynaptic boutons, the effects of suplatast tosilate on excitatory postsynaptic currents (EPSCs) were investigated with patch-clamp recording technique. We found that suplatast concentration dependently inhibited the EPSC amplitude and its frequency in single PTG neurons attached with presynaptic boutons. Thus suplatast inhibited the function of PTG neurons at both of presynaptic and postsynaptic sites.</abstract><cop>United States</cop><pmid>37283483</pmid><doi>10.1152/jn.00151.2023</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-7638-7577</orcidid><orcidid>https://orcid.org/0000-0002-9402-2838</orcidid></addata></record> |
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| source | MEDLINE; American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Arylsulfonates - pharmacology Bradykinin - pharmacology Ganglia Neurons - physiology Rats Sulfonium Compounds - pharmacology |
| title | Inhibition of suplatast tosilate on EPSC in the single paratracheal ganglion neurons attached with presynaptic boutons |
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