Injection of antibodies into grasshopper eggs as a method for studying embryonic development
Procedures are described for injecting antibodies into grasshopper eggs at stages prior to 50% of embryonic development. A microdispenser is used to permit the accurate delivery of small, predetermined volumes of antibody solution. Injected antibodies are taken up by the embryo and reach their appro...
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| Veröffentlicht in: | Development, growth & differentiation growth & differentiation, 1989-06, Vol.31 (3), p.299-305 |
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| container_end_page | 305 |
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| container_issue | 3 |
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| container_title | Development, growth & differentiation |
| container_volume | 31 |
| creator | Petrey, D Buster, D Donato, K.K Anderson, H |
| description | Procedures are described for injecting antibodies into grasshopper eggs at stages prior to 50% of embryonic development. A microdispenser is used to permit the accurate delivery of small, predetermined volumes of antibody solution. Injected antibodies are taken up by the embryo and reach their appropriate binding sites within 1 hr of injection. Antibody complexes remain stable for at least 24 hr. The method provides a useful tool for investigating developmental processes during early insect embryogenesis. |
| doi_str_mv | 10.1111/j.1440-169X.1989.00299.x |
| format | Article |
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A microdispenser is used to permit the accurate delivery of small, predetermined volumes of antibody solution. Injected antibodies are taken up by the embryo and reach their appropriate binding sites within 1 hr of injection. Antibody complexes remain stable for at least 24 hr. The method provides a useful tool for investigating developmental processes during early insect embryogenesis.</description><subject>Acrididae</subject><subject>antibodies</subject><subject>Biological and medical sciences</subject><subject>embryogenesis</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects. Development. Fetal membranes</subject><subject>Orthoptera</subject><subject>ova</subject><subject>Schistocerca americana</subject><issn>0012-1592</issn><issn>1440-169X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><recordid>eNp9kV1rFDEUhoModq3-Bc2FiDez5uQ74I202hYKXmjBCyFkZpJplpnJOJnV7r8321330nAggfc550AehDCQNZTzYbMGzkkF0vxYg9FmTQg1Zv3wBK1OwVO0IgRoBcLQM_Qi5w0hhHOgz9EZU1QDJ2qFft6MG98sMY04BezGJdapjT7jOC4Jd7PL-T5Nk5-x77qMXSk8-OU-tTikGedl2-7i2GE_1PMujbHBrf_t-zQNflxeomfB9dm_Ot7n6O7L5-8X19Xt16ubi0-3VcMYM1WrZCMCOHBaCGlAgCOCttTzhoW6MU4HrmqngvC6bmutg2ZcaBlAcC0FZ-fo_WHuNKdfW58XO8Tc-L53o0_bbKmmjBsFoAr67r8oSCW1lKKAr4_gth58a6c5Dm7e2X9fV4C3R8DlxvVhdmMT84mTRnJFoWAfD9if2PvdKQZi9ybtxu6F2b0wuzdpH03aB3t5dVkepf3NoT24ZF03lw133ygBRqhUpQz7Cylnmpw</recordid><startdate>198906</startdate><enddate>198906</enddate><creator>Petrey, D</creator><creator>Buster, D</creator><creator>Donato, K.K</creator><creator>Anderson, H</creator><general>Blackwell Publishing Ltd</general><general>Japanese Society of Developmental Biologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>198906</creationdate><title>Injection of antibodies into grasshopper eggs as a method for studying embryonic development</title><author>Petrey, D ; Buster, D ; Donato, K.K ; Anderson, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3339-d76c5f1a1a85569151a052d2e4c3fbc9a8f47ba7f5e8bdb88f834586f15486543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Acrididae</topic><topic>antibodies</topic><topic>Biological and medical sciences</topic><topic>embryogenesis</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects. Development. Fetal membranes</topic><topic>Orthoptera</topic><topic>ova</topic><topic>Schistocerca americana</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Petrey, D</creatorcontrib><creatorcontrib>Buster, D</creatorcontrib><creatorcontrib>Donato, K.K</creatorcontrib><creatorcontrib>Anderson, H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>Development, growth & differentiation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Petrey, D</au><au>Buster, D</au><au>Donato, K.K</au><au>Anderson, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Injection of antibodies into grasshopper eggs as a method for studying embryonic development</atitle><jtitle>Development, growth & differentiation</jtitle><addtitle>Dev Growth Differ</addtitle><date>1989-06</date><risdate>1989</risdate><volume>31</volume><issue>3</issue><spage>299</spage><epage>305</epage><pages>299-305</pages><issn>0012-1592</issn><eissn>1440-169X</eissn><abstract>Procedures are described for injecting antibodies into grasshopper eggs at stages prior to 50% of embryonic development. A microdispenser is used to permit the accurate delivery of small, predetermined volumes of antibody solution. Injected antibodies are taken up by the embryo and reach their appropriate binding sites within 1 hr of injection. Antibody complexes remain stable for at least 24 hr. The method provides a useful tool for investigating developmental processes during early insect embryogenesis.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>37281407</pmid><doi>10.1111/j.1440-169X.1989.00299.x</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0012-1592 |
| ispartof | Development, growth & differentiation, 1989-06, Vol.31 (3), p.299-305 |
| issn | 0012-1592 1440-169X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2823497117 |
| source | Wiley Journals; Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Acrididae antibodies Biological and medical sciences embryogenesis Embryology: invertebrates and vertebrates. Teratology Fundamental and applied biological sciences. Psychology General aspects. Development. Fetal membranes Orthoptera ova Schistocerca americana |
| title | Injection of antibodies into grasshopper eggs as a method for studying embryonic development |
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