Framework‐Hotspot Enhanced Trans Cleavage of CRISPR‐Cas12a for Clinical Samples Detection

The trans‐cleavage property of CRISPR‐Cas12a system makes it an excellent tool for disease diagnosis. Nevertheless, most methods based on CRISPR‐Cas system still require pre‐amplification of the target to achieve the desired detection sensitivity. Here we generate Framework‐Hotspot reporters (FHRs)...

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Veröffentlicht in:	Angewandte Chemie International Edition 2023-08, Vol.62 (32), p.e202305536-n/a
Hauptverfasser:	Li, Fengqin, Li, Junru, Yang, Weiqiang, Yang, Shuai, Chen, Congshuo, Du, Ling, Mei, Junyang, Tang, Qianyun, Chen, Xiaojing, Yao, Chi, Yang, Dayong, Zuo, Xiaolei, Liu, Peifeng
Format:	Artikel
Sprache:	eng
Schlagworte:	Amplification Biomarkers Biosensors Cleavage CRISPR CRISPR-Cas12a Modular construction Nucleic acids Recombination hot spots SARS-CoV-2 Signal transduction Target recognition Tetrahedral DNA Framework Trans-Cleavage
Online-Zugang:	Volltext
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