Metabolic changes associated with dark-induced leaf senescence in Arabidopsis nadk2 mutants
Arabidopsis NADK2 (NAD kinase 2) is a chloroplast-localized enzyme involved in NADP + synthesis, which acts as the final electron acceptor in the photosynthetic electron transfer chain. The NADK2-deficient mutant (nadk2) was used to analyze the effect of NAD(P)(H) unbalance in the dark-induced leaf...
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| Veröffentlicht in: | Plant signaling & behavior 2023-12, Vol.18 (1), p.2215618-2215618 |
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| creator | chaomurilege Miyagi, Atsuko Ishikawa, Toshiki Yamaguchi, Masatoshi Murayama, Hideki Kawai-Yamada, Maki |
| description | Arabidopsis NADK2 (NAD kinase 2) is a chloroplast-localized enzyme involved in NADP
+
synthesis, which acts as the final electron acceptor in the photosynthetic electron transfer chain. The NADK2-deficient mutant (nadk2) was used to analyze the effect of NAD(P)(H) unbalance in the dark-induced leaf senescence. During senescence, WT plants and nadk2 mutants showed a similar reduction in chlorophyll content. NAD(P)(H) quantification showed that the amount of total NAD(P)(H) decreased on the day 7 in WT but on the day 3 in nadk2. The phosphorylation ratio (i.e. NADP(H)/NAD(H)) decreased on day 1 in WT. In contrast, the nadk2 showed lower phosphorylation ratio at 0 day and no change throughout the aging process. Metabolome analysis showed that the metabolic profiles of both WT plants and nadk2 mutants subjected to dark-induced senescence adopted similar patterns as the senescence progressed. However, the changes in individual metabolites in the nadk2 mutants were different from those of the WT during dark-induced senescence. |
| doi_str_mv | 10.1080/15592324.2023.2215618 |
| format | Article |
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synthesis, which acts as the final electron acceptor in the photosynthetic electron transfer chain. The NADK2-deficient mutant (nadk2) was used to analyze the effect of NAD(P)(H) unbalance in the dark-induced leaf senescence. During senescence, WT plants and nadk2 mutants showed a similar reduction in chlorophyll content. NAD(P)(H) quantification showed that the amount of total NAD(P)(H) decreased on the day 7 in WT but on the day 3 in nadk2. The phosphorylation ratio (i.e. NADP(H)/NAD(H)) decreased on day 1 in WT. In contrast, the nadk2 showed lower phosphorylation ratio at 0 day and no change throughout the aging process. Metabolome analysis showed that the metabolic profiles of both WT plants and nadk2 mutants subjected to dark-induced senescence adopted similar patterns as the senescence progressed. However, the changes in individual metabolites in the nadk2 mutants were different from those of the WT during dark-induced senescence.</description><identifier>ISSN: 1559-2316</identifier><identifier>ISSN: 1559-2324</identifier><identifier>EISSN: 1559-2324</identifier><identifier>DOI: 10.1080/15592324.2023.2215618</identifier><identifier>PMID: 37272565</identifier><language>eng</language><publisher>United States: Taylor & Francis</publisher><subject>Arabidopsis ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; CE-MS ; metabolites ; NAD - metabolism ; NAD kinase ; NAD(P)(H) ; NADP - metabolism ; Plant Leaves - genetics ; Plant Leaves - metabolism ; Plant Senescence ; Research Paper</subject><ispartof>Plant signaling & behavior, 2023-12, Vol.18 (1), p.2215618-2215618</ispartof><rights>2023 The Author(s). Published with license by Taylor & Francis Group, LLC. 2023</rights><rights>2023 The Author(s). Published with license by Taylor & Francis Group, LLC. 2023 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c535t-f29dc90069200c237e4962871d3d876b53437b4626392b4542c75031c7eb2bff3</citedby><cites>FETCH-LOGICAL-c535t-f29dc90069200c237e4962871d3d876b53437b4626392b4542c75031c7eb2bff3</cites><orcidid>0000-0002-1396-5376 ; 0000-0003-4314-6555 ; 0000-0002-8732-052X ; 0000-0001-8083-6542 ; 0000-0002-5232-5793</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10324968/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10324968/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,27479,27901,27902,53766,53768,59116,59117</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37272565$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>chaomurilege</creatorcontrib><creatorcontrib>Miyagi, Atsuko</creatorcontrib><creatorcontrib>Ishikawa, Toshiki</creatorcontrib><creatorcontrib>Yamaguchi, Masatoshi</creatorcontrib><creatorcontrib>Murayama, Hideki</creatorcontrib><creatorcontrib>Kawai-Yamada, Maki</creatorcontrib><title>Metabolic changes associated with dark-induced leaf senescence in Arabidopsis nadk2 mutants</title><title>Plant signaling & behavior</title><addtitle>Plant Signal Behav</addtitle><description>Arabidopsis NADK2 (NAD kinase 2) is a chloroplast-localized enzyme involved in NADP
+
synthesis, which acts as the final electron acceptor in the photosynthetic electron transfer chain. The NADK2-deficient mutant (nadk2) was used to analyze the effect of NAD(P)(H) unbalance in the dark-induced leaf senescence. During senescence, WT plants and nadk2 mutants showed a similar reduction in chlorophyll content. NAD(P)(H) quantification showed that the amount of total NAD(P)(H) decreased on the day 7 in WT but on the day 3 in nadk2. The phosphorylation ratio (i.e. NADP(H)/NAD(H)) decreased on day 1 in WT. In contrast, the nadk2 showed lower phosphorylation ratio at 0 day and no change throughout the aging process. Metabolome analysis showed that the metabolic profiles of both WT plants and nadk2 mutants subjected to dark-induced senescence adopted similar patterns as the senescence progressed. However, the changes in individual metabolites in the nadk2 mutants were different from those of the WT during dark-induced senescence.</description><subject>Arabidopsis</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>CE-MS</subject><subject>metabolites</subject><subject>NAD - metabolism</subject><subject>NAD kinase</subject><subject>NAD(P)(H)</subject><subject>NADP - metabolism</subject><subject>Plant Leaves - genetics</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Senescence</subject><subject>Research Paper</subject><issn>1559-2316</issn><issn>1559-2324</issn><issn>1559-2324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>EIF</sourceid><sourceid>DOA</sourceid><recordid>eNp9UU1v1DAQjRCIloWfAMqRSxZ7HNvJCaqKj0pFXODEwZrYzq7bxF5sh6r_Hi-7XdELJ1szb957M6-qXlOypqQj7yjnPTBo10CArQEoF7R7Up3v682-8fT0p-KsepHSDSEtk4Q8r86YBAlc8PPq51ebcQiT07Xeot_YVGNKQTvM1tR3Lm9rg_G2cd4sulQmi2OdrLdJW69t7Xx9EXFwJuySS7VHcwv1vGT0Ob2sno04Jfvq-K6qH58-fr_80lx_-3x1eXHdaM54bkboje4JET0QooFJ2_YCOkkNM50UA2fF9tAKEKyHoeUtaMkJo1raAYZxZKvq6sBrAt6oXXQzxnsV0Km_hRA3CmN2erKKcewI6Vu0rW4lsYOAnhrowLaj6IqfVfX-wLVbhtmasmSOOD0ifdzxbqs24beipNy8F11heHtkiOHXYlNWsyvHmib0NixJFTGQpAMhCpQfoDqGlKIdTzqUqH3K6iFltU9ZHVMuc2_-NXmaeoi1AD4cAM6PIc54F-JkVMb7KcQxotcuKfZ_jT9NHrX3</recordid><startdate>20231231</startdate><enddate>20231231</enddate><creator>chaomurilege</creator><creator>Miyagi, Atsuko</creator><creator>Ishikawa, Toshiki</creator><creator>Yamaguchi, Masatoshi</creator><creator>Murayama, Hideki</creator><creator>Kawai-Yamada, Maki</creator><general>Taylor & Francis</general><general>Taylor & Francis Group</general><scope>0YH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-1396-5376</orcidid><orcidid>https://orcid.org/0000-0003-4314-6555</orcidid><orcidid>https://orcid.org/0000-0002-8732-052X</orcidid><orcidid>https://orcid.org/0000-0001-8083-6542</orcidid><orcidid>https://orcid.org/0000-0002-5232-5793</orcidid></search><sort><creationdate>20231231</creationdate><title>Metabolic changes associated with dark-induced leaf senescence in Arabidopsis nadk2 mutants</title><author>chaomurilege ; 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synthesis, which acts as the final electron acceptor in the photosynthetic electron transfer chain. The NADK2-deficient mutant (nadk2) was used to analyze the effect of NAD(P)(H) unbalance in the dark-induced leaf senescence. During senescence, WT plants and nadk2 mutants showed a similar reduction in chlorophyll content. NAD(P)(H) quantification showed that the amount of total NAD(P)(H) decreased on the day 7 in WT but on the day 3 in nadk2. The phosphorylation ratio (i.e. NADP(H)/NAD(H)) decreased on day 1 in WT. In contrast, the nadk2 showed lower phosphorylation ratio at 0 day and no change throughout the aging process. Metabolome analysis showed that the metabolic profiles of both WT plants and nadk2 mutants subjected to dark-induced senescence adopted similar patterns as the senescence progressed. However, the changes in individual metabolites in the nadk2 mutants were different from those of the WT during dark-induced senescence.</abstract><cop>United States</cop><pub>Taylor & Francis</pub><pmid>37272565</pmid><doi>10.1080/15592324.2023.2215618</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-1396-5376</orcidid><orcidid>https://orcid.org/0000-0003-4314-6555</orcidid><orcidid>https://orcid.org/0000-0002-8732-052X</orcidid><orcidid>https://orcid.org/0000-0001-8083-6542</orcidid><orcidid>https://orcid.org/0000-0002-5232-5793</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Arabidopsis Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana CE-MS metabolites NAD - metabolism NAD kinase NAD(P)(H) NADP - metabolism Plant Leaves - genetics Plant Leaves - metabolism Plant Senescence Research Paper |
| title | Metabolic changes associated with dark-induced leaf senescence in Arabidopsis nadk2 mutants |
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