A splinkerette PCR-based genome walking technique for the identification of transgene integration sites in CHO cells

Identification of recombinant gene integrations sites in the Chinese hamster ovary (CHO) cell genome is increasingly important to assure monoclonality. While next-generation sequencing (NGS) is commonly used for the gene integration site analysis, it is a time-consuming and costly technique as it an...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Journal of biotechnology 2023-07, Vol.371-372, p.1-9
Hauptverfasser:	Han, Hye-Jin, Kim, Dae Hoon, Baik, Jong Youn
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell line development Chinese hamster ovary (CHO) cells Gene integration site analysis Splinkerette adaptor Splinkerette-PCR (spPCR)
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!