Anticonvulsant Role of Adenosine is Blunted During Alcohol Withdrawal Syndrome in an Adult Zebrafish Model
Alcohol (ethanol) dependence and related disorders are life-threatening conditions and source of suffering for the user, family members and society. Alcohol withdrawal syndrome (AWS) is a little-known dynamic process associated with a high frequency of relapses. A state of hyperglutamatergic neurotr...
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| Veröffentlicht in: | Neurochemical research 2023-10, Vol.48 (10), p.3007-3015 |
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| creator | Couto, Giovanna Trevisan da Silva, Guilherme Pietro Rockenbach, Liliana da Silva, Jéssica Scheid Vianna, Monica Ryff Moreira Roca Da Silva, Rosane Souza |
| description | Alcohol (ethanol) dependence and related disorders are life-threatening conditions and source of suffering for the user, family members and society. Alcohol withdrawal syndrome (AWS) is a little-known dynamic process associated with a high frequency of relapses. A state of hyperglutamatergic neurotransmission and imbalanced GABAergic function is related to an increased susceptibility to seizures during alcohol withdrawal. Adenosine signaling display an important role in endogenous response to decrease seizure and related damages. Here, an intermittent alcohol exposure regimen (1 h daily of 0.5% ethanol solution) for 16 days or 8 days of the same ethanol exposure regimen followed by 1 or 8 days of ethanol withdrawal was used to assess adenosine signaling in the context of seizure susceptibility using adult zebrafish. In both abstainer groups, a sub-convulsant dose of pentylenetetrazol (2.5 mM) was able to increase the frequency of animals reaching a clonic seizure-like state, while continuous-treated animals had no seizure, as did control animals. The total brain mRNA expression of A
1
adenosine receptor was decreased in animals with 1 day of ethanol withdrawal. The agonism of A
1
adenosine receptor induced an anticonvulsant effect in animals with 1 day of ethanol withdrawal after the injection of the specific agonist (N
6
-cyclopentyladenosine, 10 mg.Kg
− 1
; i.p.). These findings reinforce A
1
adenosine receptor as a key target in acute alcohol withdrawal syndrome and zebrafish as an excellent platform to study biological mechanism of AWS. |
| doi_str_mv | 10.1007/s11064-023-03958-0 |
| format | Article |
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1
adenosine receptor was decreased in animals with 1 day of ethanol withdrawal. The agonism of A
1
adenosine receptor induced an anticonvulsant effect in animals with 1 day of ethanol withdrawal after the injection of the specific agonist (N
6
-cyclopentyladenosine, 10 mg.Kg
− 1
; i.p.). These findings reinforce A
1
adenosine receptor as a key target in acute alcohol withdrawal syndrome and zebrafish as an excellent platform to study biological mechanism of AWS.</description><identifier>ISSN: 0364-3190</identifier><identifier>EISSN: 1573-6903</identifier><identifier>DOI: 10.1007/s11064-023-03958-0</identifier><identifier>PMID: 37256498</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adenosine ; Alcohol withdrawal ; Alcohols ; Animals ; Anticonvulsants ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Danio rerio ; Ethanol ; Gene expression ; Neurochemistry ; Neurology ; Neurosciences ; Neurotransmission ; Original Paper ; Receptors ; Seizures ; Zebrafish ; γ-Aminobutyric acid</subject><ispartof>Neurochemical research, 2023-10, Vol.48 (10), p.3007-3015</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-8baefd969ee19d60c92a66494b83bbd585cd23102398f0be4fb971404b2b812e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11064-023-03958-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11064-023-03958-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37256498$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Couto, Giovanna Trevisan</creatorcontrib><creatorcontrib>da Silva, Guilherme Pietro</creatorcontrib><creatorcontrib>Rockenbach, Liliana</creatorcontrib><creatorcontrib>da Silva, Jéssica Scheid</creatorcontrib><creatorcontrib>Vianna, Monica Ryff Moreira Roca</creatorcontrib><creatorcontrib>Da Silva, Rosane Souza</creatorcontrib><title>Anticonvulsant Role of Adenosine is Blunted During Alcohol Withdrawal Syndrome in an Adult Zebrafish Model</title><title>Neurochemical research</title><addtitle>Neurochem Res</addtitle><addtitle>Neurochem Res</addtitle><description>Alcohol (ethanol) dependence and related disorders are life-threatening conditions and source of suffering for the user, family members and society. Alcohol withdrawal syndrome (AWS) is a little-known dynamic process associated with a high frequency of relapses. A state of hyperglutamatergic neurotransmission and imbalanced GABAergic function is related to an increased susceptibility to seizures during alcohol withdrawal. Adenosine signaling display an important role in endogenous response to decrease seizure and related damages. Here, an intermittent alcohol exposure regimen (1 h daily of 0.5% ethanol solution) for 16 days or 8 days of the same ethanol exposure regimen followed by 1 or 8 days of ethanol withdrawal was used to assess adenosine signaling in the context of seizure susceptibility using adult zebrafish. In both abstainer groups, a sub-convulsant dose of pentylenetetrazol (2.5 mM) was able to increase the frequency of animals reaching a clonic seizure-like state, while continuous-treated animals had no seizure, as did control animals. The total brain mRNA expression of A
1
adenosine receptor was decreased in animals with 1 day of ethanol withdrawal. The agonism of A
1
adenosine receptor induced an anticonvulsant effect in animals with 1 day of ethanol withdrawal after the injection of the specific agonist (N
6
-cyclopentyladenosine, 10 mg.Kg
− 1
; i.p.). These findings reinforce A
1
adenosine receptor as a key target in acute alcohol withdrawal syndrome and zebrafish as an excellent platform to study biological mechanism of AWS.</description><subject>Adenosine</subject><subject>Alcohol withdrawal</subject><subject>Alcohols</subject><subject>Animals</subject><subject>Anticonvulsants</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Danio rerio</subject><subject>Ethanol</subject><subject>Gene expression</subject><subject>Neurochemistry</subject><subject>Neurology</subject><subject>Neurosciences</subject><subject>Neurotransmission</subject><subject>Original Paper</subject><subject>Receptors</subject><subject>Seizures</subject><subject>Zebrafish</subject><subject>γ-Aminobutyric acid</subject><issn>0364-3190</issn><issn>1573-6903</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kUtPHDEQhK0oKGwIfyAHZCkXLgNte14-bpYEkECR8lAkLpY97snOymsv9gwR_x5vloeUA6c--KvqchchHxmcMIDmNDEGdVkAFwUIWbUFvCEzVjWiqCWIt2QGIj8LJmGfvE9pBZBlnL0j-6LhVV3KdkZWcz8OXfB3k0vaj_R7cEhDT-cWfUiDRzok-tlNfkRLz6Y4-D907rqwDI7-HsaljfqvdvTHvbcxrDPtqfZZPbmR3qCJuh_Skl4Hi-4D2eu1S3j4OA_Ir69ffi4uiqtv55eL-VXRCV6PRWs09lbWEpFJW0Mnua5z2NK0whhbtVVnuWD517LtwWDZG9mwEkrDTcs4igNyvPPdxHA7YRrVekgdOqc9hikp3nImSmhKltFP_6GrMEWf02WqkqJpKg6Z4juqiyGliL3axGGt471ioLZNqF0TKmdS_5pQW9HRo_Vk1mifJU-nz4DYAWmzvSrGl92v2D4AijWTSA</recordid><startdate>20231001</startdate><enddate>20231001</enddate><creator>Couto, Giovanna Trevisan</creator><creator>da Silva, Guilherme Pietro</creator><creator>Rockenbach, Liliana</creator><creator>da Silva, Jéssica Scheid</creator><creator>Vianna, Monica Ryff Moreira Roca</creator><creator>Da Silva, Rosane Souza</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20231001</creationdate><title>Anticonvulsant Role of Adenosine is Blunted During Alcohol Withdrawal Syndrome in an Adult Zebrafish Model</title><author>Couto, Giovanna Trevisan ; 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Alcohol withdrawal syndrome (AWS) is a little-known dynamic process associated with a high frequency of relapses. A state of hyperglutamatergic neurotransmission and imbalanced GABAergic function is related to an increased susceptibility to seizures during alcohol withdrawal. Adenosine signaling display an important role in endogenous response to decrease seizure and related damages. Here, an intermittent alcohol exposure regimen (1 h daily of 0.5% ethanol solution) for 16 days or 8 days of the same ethanol exposure regimen followed by 1 or 8 days of ethanol withdrawal was used to assess adenosine signaling in the context of seizure susceptibility using adult zebrafish. In both abstainer groups, a sub-convulsant dose of pentylenetetrazol (2.5 mM) was able to increase the frequency of animals reaching a clonic seizure-like state, while continuous-treated animals had no seizure, as did control animals. The total brain mRNA expression of A
1
adenosine receptor was decreased in animals with 1 day of ethanol withdrawal. The agonism of A
1
adenosine receptor induced an anticonvulsant effect in animals with 1 day of ethanol withdrawal after the injection of the specific agonist (N
6
-cyclopentyladenosine, 10 mg.Kg
− 1
; i.p.). These findings reinforce A
1
adenosine receptor as a key target in acute alcohol withdrawal syndrome and zebrafish as an excellent platform to study biological mechanism of AWS.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>37256498</pmid><doi>10.1007/s11064-023-03958-0</doi><tpages>9</tpages></addata></record> |
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| subjects | Adenosine Alcohol withdrawal Alcohols Animals Anticonvulsants Biochemistry Biomedical and Life Sciences Biomedicine Cell Biology Danio rerio Ethanol Gene expression Neurochemistry Neurology Neurosciences Neurotransmission Original Paper Receptors Seizures Zebrafish γ-Aminobutyric acid |
| title | Anticonvulsant Role of Adenosine is Blunted During Alcohol Withdrawal Syndrome in an Adult Zebrafish Model |
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