A Versatile PdRu Bimetallic Nanoenzyme-Integrated Enzyme-Linked Immunosorbent Assay for Highly Sensitive Escherichia coli O157:H7 Detection
Nanozymes have drawn much attention as an enzyme mimetic with low cost and stability in enhancing analytical performance. Herein, a peroxidase-mimicking nanozyme-improved enzyme-linked immunosorbent assay (ELISA) was developed employing the bimetallic PdRu nanozyme to replace the natural enzymes as...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2023-06, Vol.95 (24), p.9237-9243 |
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| creator | Wang, Ying Bu, Tong Cao, Yuanyuan Wu, Haiyu Xi, Jia Feng, Qinlin Xuan, Chenyu Wang, Li |
| description | Nanozymes have drawn much attention as an enzyme mimetic with low cost and stability in enhancing analytical performance. Herein, a peroxidase-mimicking nanozyme-improved enzyme-linked immunosorbent assay (ELISA) was developed employing the bimetallic PdRu nanozyme to replace the natural enzymes as a catalytic carrier for the sensing of Escherichia coli O157:H7 (E. coli O157:H7). The PdRu nanozyme displayed ultrahigh catalytic activity, possessing a catalytic rate that was 5-fold higher than horseradish peroxidase (HRP). In addition, PdRu exhibited great biological affinity with antibody (affinity constant was about 6.75 × 1012 M) and high stability. All those advantages ensure the successful establishment and the construction of a novel colorimetric biosensor for E. coli O157:H7 detection. PdRu-based ELISA not only achieved an ultrasensitive detection sensitivity (8.7 × 102 CFU/mL) by approximately 288-fold as compared to the traditional HRP-based ELISA and also possessed satisfactory specificity and reproducibility (relative standard deviation (RSD) < 10%). Furthermore, the feasibility of PdRu-ELISA was further evaluated by detecting E. coli O157:H7 in actual samples with satisfactory recoveries, indicating its potential for applications in bioassays and clinical diagnostics. |
| doi_str_mv | 10.1021/acs.analchem.3c00743 |
| format | Article |
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Herein, a peroxidase-mimicking nanozyme-improved enzyme-linked immunosorbent assay (ELISA) was developed employing the bimetallic PdRu nanozyme to replace the natural enzymes as a catalytic carrier for the sensing of Escherichia coli O157:H7 (E. coli O157:H7). The PdRu nanozyme displayed ultrahigh catalytic activity, possessing a catalytic rate that was 5-fold higher than horseradish peroxidase (HRP). In addition, PdRu exhibited great biological affinity with antibody (affinity constant was about 6.75 × 1012 M) and high stability. All those advantages ensure the successful establishment and the construction of a novel colorimetric biosensor for E. coli O157:H7 detection. PdRu-based ELISA not only achieved an ultrasensitive detection sensitivity (8.7 × 102 CFU/mL) by approximately 288-fold as compared to the traditional HRP-based ELISA and also possessed satisfactory specificity and reproducibility (relative standard deviation (RSD) < 10%). Furthermore, the feasibility of PdRu-ELISA was further evaluated by detecting E. coli O157:H7 in actual samples with satisfactory recoveries, indicating its potential for applications in bioassays and clinical diagnostics.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.3c00743</identifier><identifier>PMID: 37232263</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Affinity ; Analytical chemistry ; Antibodies ; Antibodies, Bacterial ; Bimetals ; Bioassays ; Biosensors ; Catalytic activity ; Chemistry ; Colorimetry ; Cost analysis ; E coli ; Enzyme-Linked Immunosorbent Assay ; Enzymes ; Escherichia coli ; Escherichia coli O157 ; Horseradish Peroxidase ; Peroxidase ; Reproducibility of Results ; Stability analysis</subject><ispartof>Analytical chemistry (Washington), 2023-06, Vol.95 (24), p.9237-9243</ispartof><rights>2023 American Chemical Society</rights><rights>Copyright American Chemical Society Jun 20, 2023</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a376t-2106377b1c77fd860bb6e82a25e3139902a132b53225b5fe6ec71ec61b42a34a3</citedby><cites>FETCH-LOGICAL-a376t-2106377b1c77fd860bb6e82a25e3139902a132b53225b5fe6ec71ec61b42a34a3</cites><orcidid>0000-0003-3984-2494</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.3c00743$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.3c00743$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37232263$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Ying</creatorcontrib><creatorcontrib>Bu, Tong</creatorcontrib><creatorcontrib>Cao, Yuanyuan</creatorcontrib><creatorcontrib>Wu, Haiyu</creatorcontrib><creatorcontrib>Xi, Jia</creatorcontrib><creatorcontrib>Feng, Qinlin</creatorcontrib><creatorcontrib>Xuan, Chenyu</creatorcontrib><creatorcontrib>Wang, Li</creatorcontrib><title>A Versatile PdRu Bimetallic Nanoenzyme-Integrated Enzyme-Linked Immunosorbent Assay for Highly Sensitive Escherichia coli O157:H7 Detection</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Nanozymes have drawn much attention as an enzyme mimetic with low cost and stability in enhancing analytical performance. Herein, a peroxidase-mimicking nanozyme-improved enzyme-linked immunosorbent assay (ELISA) was developed employing the bimetallic PdRu nanozyme to replace the natural enzymes as a catalytic carrier for the sensing of Escherichia coli O157:H7 (E. coli O157:H7). The PdRu nanozyme displayed ultrahigh catalytic activity, possessing a catalytic rate that was 5-fold higher than horseradish peroxidase (HRP). In addition, PdRu exhibited great biological affinity with antibody (affinity constant was about 6.75 × 1012 M) and high stability. All those advantages ensure the successful establishment and the construction of a novel colorimetric biosensor for E. coli O157:H7 detection. PdRu-based ELISA not only achieved an ultrasensitive detection sensitivity (8.7 × 102 CFU/mL) by approximately 288-fold as compared to the traditional HRP-based ELISA and also possessed satisfactory specificity and reproducibility (relative standard deviation (RSD) < 10%). Furthermore, the feasibility of PdRu-ELISA was further evaluated by detecting E. coli O157:H7 in actual samples with satisfactory recoveries, indicating its potential for applications in bioassays and clinical diagnostics.</description><subject>Affinity</subject><subject>Analytical chemistry</subject><subject>Antibodies</subject><subject>Antibodies, Bacterial</subject><subject>Bimetals</subject><subject>Bioassays</subject><subject>Biosensors</subject><subject>Catalytic activity</subject><subject>Chemistry</subject><subject>Colorimetry</subject><subject>Cost analysis</subject><subject>E coli</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Enzymes</subject><subject>Escherichia coli</subject><subject>Escherichia coli O157</subject><subject>Horseradish Peroxidase</subject><subject>Peroxidase</subject><subject>Reproducibility of Results</subject><subject>Stability analysis</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1uEzEURi0EoqHwBghZYsNmwrU9Y0_YpW1oIkUt4m878jh3GpcZu9ieSuEVeOm6JO2CRVeWrfN9vvYh5C2DKQPOPmoTp9rp3mxxmAoDoErxjExYxaGQdc2fkwkAiIIrgCPyKsZrAMaAyZfkSCguOJdiQv7O6U8MUSfbI_2y-TrSEztg0n1vDb3QzqP7sxuwWLmEV0En3NDF_mRt3a-8Ww3D6Hz0oUWX6DxGvaOdD3Rpr7b9jn5DF22yt0gXMU8arNlaTY3vLb1klfq0VPQME5pkvXtNXnS6j_jmsB6TH58X30-XxfryfHU6XxdaKJkKzkAKpVpmlOo2tYS2lVhzzSsUTMxmwDUTvK3yC6u26lCiUQyNZG3JtSi1OCYf9r03wf8eMaZmsNFg32uHfowNrzkAn7Gyzuj7_9BrP4b86_8oNatLznimyj1lgo8xYNfcBDvosGsYNPeymiyreZDVHGTl2LtD-dgOuHkMPdjJAOyB-_jjxU923gF2c6NK</recordid><startdate>20230620</startdate><enddate>20230620</enddate><creator>Wang, Ying</creator><creator>Bu, Tong</creator><creator>Cao, Yuanyuan</creator><creator>Wu, Haiyu</creator><creator>Xi, Jia</creator><creator>Feng, Qinlin</creator><creator>Xuan, Chenyu</creator><creator>Wang, Li</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3984-2494</orcidid></search><sort><creationdate>20230620</creationdate><title>A Versatile PdRu Bimetallic Nanoenzyme-Integrated Enzyme-Linked Immunosorbent Assay for Highly Sensitive Escherichia coli O157:H7 Detection</title><author>Wang, Ying ; Bu, Tong ; Cao, Yuanyuan ; Wu, Haiyu ; Xi, Jia ; Feng, Qinlin ; Xuan, Chenyu ; Wang, Li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a376t-2106377b1c77fd860bb6e82a25e3139902a132b53225b5fe6ec71ec61b42a34a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Affinity</topic><topic>Analytical chemistry</topic><topic>Antibodies</topic><topic>Antibodies, Bacterial</topic><topic>Bimetals</topic><topic>Bioassays</topic><topic>Biosensors</topic><topic>Catalytic activity</topic><topic>Chemistry</topic><topic>Colorimetry</topic><topic>Cost analysis</topic><topic>E coli</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Enzymes</topic><topic>Escherichia coli</topic><topic>Escherichia coli O157</topic><topic>Horseradish Peroxidase</topic><topic>Peroxidase</topic><topic>Reproducibility of Results</topic><topic>Stability analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Ying</creatorcontrib><creatorcontrib>Bu, Tong</creatorcontrib><creatorcontrib>Cao, Yuanyuan</creatorcontrib><creatorcontrib>Wu, Haiyu</creatorcontrib><creatorcontrib>Xi, Jia</creatorcontrib><creatorcontrib>Feng, Qinlin</creatorcontrib><creatorcontrib>Xuan, Chenyu</creatorcontrib><creatorcontrib>Wang, Li</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Ying</au><au>Bu, Tong</au><au>Cao, Yuanyuan</au><au>Wu, Haiyu</au><au>Xi, Jia</au><au>Feng, Qinlin</au><au>Xuan, Chenyu</au><au>Wang, Li</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Versatile PdRu Bimetallic Nanoenzyme-Integrated Enzyme-Linked Immunosorbent Assay for Highly Sensitive Escherichia coli O157:H7 Detection</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2023-06-20</date><risdate>2023</risdate><volume>95</volume><issue>24</issue><spage>9237</spage><epage>9243</epage><pages>9237-9243</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>Nanozymes have drawn much attention as an enzyme mimetic with low cost and stability in enhancing analytical performance. Herein, a peroxidase-mimicking nanozyme-improved enzyme-linked immunosorbent assay (ELISA) was developed employing the bimetallic PdRu nanozyme to replace the natural enzymes as a catalytic carrier for the sensing of Escherichia coli O157:H7 (E. coli O157:H7). The PdRu nanozyme displayed ultrahigh catalytic activity, possessing a catalytic rate that was 5-fold higher than horseradish peroxidase (HRP). In addition, PdRu exhibited great biological affinity with antibody (affinity constant was about 6.75 × 1012 M) and high stability. All those advantages ensure the successful establishment and the construction of a novel colorimetric biosensor for E. coli O157:H7 detection. PdRu-based ELISA not only achieved an ultrasensitive detection sensitivity (8.7 × 102 CFU/mL) by approximately 288-fold as compared to the traditional HRP-based ELISA and also possessed satisfactory specificity and reproducibility (relative standard deviation (RSD) < 10%). Furthermore, the feasibility of PdRu-ELISA was further evaluated by detecting E. coli O157:H7 in actual samples with satisfactory recoveries, indicating its potential for applications in bioassays and clinical diagnostics.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>37232263</pmid><doi>10.1021/acs.analchem.3c00743</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-3984-2494</orcidid></addata></record> |
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| subjects | Affinity Analytical chemistry Antibodies Antibodies, Bacterial Bimetals Bioassays Biosensors Catalytic activity Chemistry Colorimetry Cost analysis E coli Enzyme-Linked Immunosorbent Assay Enzymes Escherichia coli Escherichia coli O157 Horseradish Peroxidase Peroxidase Reproducibility of Results Stability analysis |
| title | A Versatile PdRu Bimetallic Nanoenzyme-Integrated Enzyme-Linked Immunosorbent Assay for Highly Sensitive Escherichia coli O157:H7 Detection |
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