Photobiomodulation, alone or combined with adipose-derived stem cells, reduces inflammation by modulation of microRNA-146a and interleukin-1ß in a delayed-healing infected wound in diabetic rats
Diabetic wounds are categorized by chronic inflammation, leading to the development of diabetic foot ulcers, which cause amputation and death. Herewith, we examined the effect of photobiomodulation (PBM) plus allogeneic diabetic adipose tissue-derived stem cells (ad-ADS) on stereological parameters...
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| Veröffentlicht in: | Lasers in medical science 2023-05, Vol.38 (1), p.129-129, Article 129 |
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| creator | Moravej, Fahimeh Ghasemi Amini, Abdollah Masteri Farahani, Reza Mohammadi-Yeganeh, Samira Mostafavinia, Atarodalsadat Ahmadi, Houssein Omidi, Hamidreza Rezaei, Fatemehalsadat Gachkar, Latif Hamblin, Michael R Chien, Sufan Bayat, Mohammad |
| description | Diabetic wounds are categorized by chronic inflammation, leading to the development of diabetic foot ulcers, which cause amputation and death. Herewith, we examined the effect of photobiomodulation (PBM) plus allogeneic diabetic adipose tissue-derived stem cells (ad-ADS) on stereological parameters and expression levels of interleukin (IL)-1ß and microRNA (miRNA)-146a in the inflammatory (day 4) and proliferation (day 8) stages of wound healing in an ischemic infected (with 2×10
7
colony-forming units of methicillin-resistant
Staphylococcus aureus
) delayed healing wound model (IIDHWM) in type I diabetic (TIDM) rats. There were five groups of rats: group 1 control (C); group 2 (CELL) in which rat wounds received 1×10
6
ad-ADS; group 3 (CL) in which rat wounds received the ad-ADS and were subsequently exposed to PBM(890 nm, 80 Hz, 3.5 J/cm
2
, in vivo); group 4 (CP) in which the ad-ADS preconditioned by the PBM(630 nm + 810 nm, 0.05 W, 1.2 J/cm
2
, 3 times) were implanted into rat wounds; group 5 (CLP) in which the PBM preconditioned ad-ADS were implanted into rat wounds, which were then exposed to PBM. On both days, significantly better histological results were seen in all experimental groups except control. Significantly better histological results were observed in the ad-ADS plus PBM treatment correlated to the ad-ADS alone group (
p |
| doi_str_mv | 10.1007/s10103-023-03786-2 |
| format | Article |
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7
colony-forming units of methicillin-resistant
Staphylococcus aureus
) delayed healing wound model (IIDHWM) in type I diabetic (TIDM) rats. There were five groups of rats: group 1 control (C); group 2 (CELL) in which rat wounds received 1×10
6
ad-ADS; group 3 (CL) in which rat wounds received the ad-ADS and were subsequently exposed to PBM(890 nm, 80 Hz, 3.5 J/cm
2
, in vivo); group 4 (CP) in which the ad-ADS preconditioned by the PBM(630 nm + 810 nm, 0.05 W, 1.2 J/cm
2
, 3 times) were implanted into rat wounds; group 5 (CLP) in which the PBM preconditioned ad-ADS were implanted into rat wounds, which were then exposed to PBM. On both days, significantly better histological results were seen in all experimental groups except control. Significantly better histological results were observed in the ad-ADS plus PBM treatment correlated to the ad-ADS alone group (
p
<0.05). Overall, PBM preconditioned ad-ADS followed by PBM of the wound showed the most significant improvement in histological measures correlated to the other experimental groups (
p
<0.05). On days 4 and 8, IL-1 β levels of all experimental groups were lower than the control group; however, on day 8, only the CLP group was different (
p
<0.01). On day 4, miR-146a expression levels were substantially greater in the CLP and CELL groups correlated to the other groups, on day 8 miR-146a in all treatment groups was upper than C (
p
<0.01). ad-ADS plus PBM, ad-ADS, and PBM all improved the inflammatory phase of wound healing in an IIDHWM in TIDM1 rats by reducing inflammatory cells (neutrophils, macrophages) and IL-1ß, and increasing miRNA-146a. The ad-ADS+PBM combination was better than either ad-ADS or PBM alone, because of the higher proliferative and anti-inflammatory effects of the PBM+ad-ADS regimen.</description><identifier>ISSN: 1435-604X</identifier><identifier>ISSN: 0268-8921</identifier><identifier>EISSN: 1435-604X</identifier><identifier>DOI: 10.1007/s10103-023-03786-2</identifier><identifier>PMID: 37243832</identifier><language>eng</language><publisher>London: Springer London</publisher><subject>Adipose tissue ; Amputation ; Animals ; Cytokines ; Dentistry ; Diabetes ; Diabetes mellitus ; Diabetes Mellitus, Experimental - pathology ; Drug resistance ; Inflammation ; Inflammation - radiotherapy ; Interleukin 1 ; Ischemia ; Lasers ; Leukocytes (neutrophilic) ; Light therapy ; Low-Level Light Therapy - methods ; Macrophages ; Medicine ; Medicine & Public Health ; Methicillin ; Methicillin-Resistant Staphylococcus aureus ; MicroRNAs ; MicroRNAs - genetics ; miRNA ; Optical Devices ; Optics ; Original Article ; Photonics ; Quantum Optics ; Rats ; Rats, Wistar ; Ribonucleic acid ; RNA ; Staphylococcus infections ; Stem cells ; Stem Cells - pathology ; Ulcers ; Wound Healing</subject><ispartof>Lasers in medical science, 2023-05, Vol.38 (1), p.129-129, Article 129</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-d6762f4ccda1d101ee1687a3ec37adddd7855811661b10db4fe28285f1e51f6c3</citedby><cites>FETCH-LOGICAL-c375t-d6762f4ccda1d101ee1687a3ec37adddd7855811661b10db4fe28285f1e51f6c3</cites><orcidid>0000-0001-8921-4045 ; 0000-0002-5314-5022 ; 0000-0001-6431-4605 ; 0000-0002-9408-3200 ; 0000-0001-6721-2153 ; 0000-0003-2270-5637 ; 0000-0001-8748-1270 ; 0000-0001-5214-4223 ; 0000-0003-3878-3082 ; 0000-0002-1067-4178 ; 0000-0002-6300-4143 ; 0000-0001-5699-9907</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10103-023-03786-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10103-023-03786-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37243832$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moravej, Fahimeh Ghasemi</creatorcontrib><creatorcontrib>Amini, Abdollah</creatorcontrib><creatorcontrib>Masteri Farahani, Reza</creatorcontrib><creatorcontrib>Mohammadi-Yeganeh, Samira</creatorcontrib><creatorcontrib>Mostafavinia, Atarodalsadat</creatorcontrib><creatorcontrib>Ahmadi, Houssein</creatorcontrib><creatorcontrib>Omidi, Hamidreza</creatorcontrib><creatorcontrib>Rezaei, Fatemehalsadat</creatorcontrib><creatorcontrib>Gachkar, Latif</creatorcontrib><creatorcontrib>Hamblin, Michael R</creatorcontrib><creatorcontrib>Chien, Sufan</creatorcontrib><creatorcontrib>Bayat, Mohammad</creatorcontrib><title>Photobiomodulation, alone or combined with adipose-derived stem cells, reduces inflammation by modulation of microRNA-146a and interleukin-1ß in a delayed-healing infected wound in diabetic rats</title><title>Lasers in medical science</title><addtitle>Lasers Med Sci</addtitle><addtitle>Lasers Med Sci</addtitle><description>Diabetic wounds are categorized by chronic inflammation, leading to the development of diabetic foot ulcers, which cause amputation and death. Herewith, we examined the effect of photobiomodulation (PBM) plus allogeneic diabetic adipose tissue-derived stem cells (ad-ADS) on stereological parameters and expression levels of interleukin (IL)-1ß and microRNA (miRNA)-146a in the inflammatory (day 4) and proliferation (day 8) stages of wound healing in an ischemic infected (with 2×10
7
colony-forming units of methicillin-resistant
Staphylococcus aureus
) delayed healing wound model (IIDHWM) in type I diabetic (TIDM) rats. There were five groups of rats: group 1 control (C); group 2 (CELL) in which rat wounds received 1×10
6
ad-ADS; group 3 (CL) in which rat wounds received the ad-ADS and were subsequently exposed to PBM(890 nm, 80 Hz, 3.5 J/cm
2
, in vivo); group 4 (CP) in which the ad-ADS preconditioned by the PBM(630 nm + 810 nm, 0.05 W, 1.2 J/cm
2
, 3 times) were implanted into rat wounds; group 5 (CLP) in which the PBM preconditioned ad-ADS were implanted into rat wounds, which were then exposed to PBM. On both days, significantly better histological results were seen in all experimental groups except control. Significantly better histological results were observed in the ad-ADS plus PBM treatment correlated to the ad-ADS alone group (
p
<0.05). Overall, PBM preconditioned ad-ADS followed by PBM of the wound showed the most significant improvement in histological measures correlated to the other experimental groups (
p
<0.05). On days 4 and 8, IL-1 β levels of all experimental groups were lower than the control group; however, on day 8, only the CLP group was different (
p
<0.01). On day 4, miR-146a expression levels were substantially greater in the CLP and CELL groups correlated to the other groups, on day 8 miR-146a in all treatment groups was upper than C (
p
<0.01). ad-ADS plus PBM, ad-ADS, and PBM all improved the inflammatory phase of wound healing in an IIDHWM in TIDM1 rats by reducing inflammatory cells (neutrophils, macrophages) and IL-1ß, and increasing miRNA-146a. The ad-ADS+PBM combination was better than either ad-ADS or PBM alone, because of the higher proliferative and anti-inflammatory effects of the PBM+ad-ADS regimen.</description><subject>Adipose tissue</subject><subject>Amputation</subject><subject>Animals</subject><subject>Cytokines</subject><subject>Dentistry</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetes Mellitus, Experimental - pathology</subject><subject>Drug resistance</subject><subject>Inflammation</subject><subject>Inflammation - radiotherapy</subject><subject>Interleukin 1</subject><subject>Ischemia</subject><subject>Lasers</subject><subject>Leukocytes (neutrophilic)</subject><subject>Light therapy</subject><subject>Low-Level Light Therapy - methods</subject><subject>Macrophages</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Methicillin</subject><subject>Methicillin-Resistant Staphylococcus aureus</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>miRNA</subject><subject>Optical Devices</subject><subject>Optics</subject><subject>Original Article</subject><subject>Photonics</subject><subject>Quantum Optics</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Staphylococcus infections</subject><subject>Stem cells</subject><subject>Stem Cells - pathology</subject><subject>Ulcers</subject><subject>Wound Healing</subject><issn>1435-604X</issn><issn>0268-8921</issn><issn>1435-604X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc1u1DAQxyMEoqXwAhyQJS4c6uKxEzt7rCq-pKogBBK3yLEnXZfEXmyn1T4ND9MXw9ktFHHAkuWP-c3f4_lX1XNgJ8CYep2AAROU8TKFaiXlD6pDqEVDJau_Pfxrf1A9SemKMVASxOPqQChei1bww-r20zrk0LswBTuPOrvgj4keg0cSIjFh6p1HS25cXhNt3SYkpBajuy6XKeNEDI5jOiYR7WwwEeeHUU_TToj0W3IvS8JAJmdi-HxxSqGWmmhvC58xjjh_d57C7c9yJppYHPUWLV2jHp2_XETR5KWMMO9yiHW6x-wMiTqnp9WjQY8Jn92tR9XXt2--nL2n5x_ffTg7PadGqCZTK5XkQ22M1WBL6xBBtkoLLGFty1Bt07QAUkIPzPb1gLzlbTMANjBII46qV3vdTQw_Zky5m1xa_q89hjl1hWaMqxXnBX35D3oV5uhLdYWClZKiXq0KxfdU6UpKEYduE92k47YD1i0Wd3uLu2Jxt7O4W6Rf3EnP_YT2T8pvTwsg9kAqIX-J8f7t_8j-AtNGtWw</recordid><startdate>20230527</startdate><enddate>20230527</enddate><creator>Moravej, Fahimeh Ghasemi</creator><creator>Amini, Abdollah</creator><creator>Masteri Farahani, Reza</creator><creator>Mohammadi-Yeganeh, Samira</creator><creator>Mostafavinia, Atarodalsadat</creator><creator>Ahmadi, Houssein</creator><creator>Omidi, Hamidreza</creator><creator>Rezaei, Fatemehalsadat</creator><creator>Gachkar, Latif</creator><creator>Hamblin, Michael R</creator><creator>Chien, Sufan</creator><creator>Bayat, Mohammad</creator><general>Springer London</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7RV</scope><scope>7SP</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8921-4045</orcidid><orcidid>https://orcid.org/0000-0002-5314-5022</orcidid><orcidid>https://orcid.org/0000-0001-6431-4605</orcidid><orcidid>https://orcid.org/0000-0002-9408-3200</orcidid><orcidid>https://orcid.org/0000-0001-6721-2153</orcidid><orcidid>https://orcid.org/0000-0003-2270-5637</orcidid><orcidid>https://orcid.org/0000-0001-8748-1270</orcidid><orcidid>https://orcid.org/0000-0001-5214-4223</orcidid><orcidid>https://orcid.org/0000-0003-3878-3082</orcidid><orcidid>https://orcid.org/0000-0002-1067-4178</orcidid><orcidid>https://orcid.org/0000-0002-6300-4143</orcidid><orcidid>https://orcid.org/0000-0001-5699-9907</orcidid></search><sort><creationdate>20230527</creationdate><title>Photobiomodulation, alone or combined with adipose-derived stem cells, reduces inflammation by modulation of microRNA-146a and interleukin-1ß in a delayed-healing infected wound in diabetic rats</title><author>Moravej, Fahimeh Ghasemi ; Amini, Abdollah ; Masteri Farahani, Reza ; Mohammadi-Yeganeh, Samira ; Mostafavinia, Atarodalsadat ; Ahmadi, Houssein ; Omidi, Hamidreza ; Rezaei, Fatemehalsadat ; Gachkar, Latif ; Hamblin, Michael R ; Chien, Sufan ; Bayat, Mohammad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-d6762f4ccda1d101ee1687a3ec37adddd7855811661b10db4fe28285f1e51f6c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Adipose tissue</topic><topic>Amputation</topic><topic>Animals</topic><topic>Cytokines</topic><topic>Dentistry</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Diabetes Mellitus, Experimental - pathology</topic><topic>Drug resistance</topic><topic>Inflammation</topic><topic>Inflammation - radiotherapy</topic><topic>Interleukin 1</topic><topic>Ischemia</topic><topic>Lasers</topic><topic>Leukocytes (neutrophilic)</topic><topic>Light therapy</topic><topic>Low-Level Light Therapy - methods</topic><topic>Macrophages</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Methicillin</topic><topic>Methicillin-Resistant Staphylococcus aureus</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>miRNA</topic><topic>Optical Devices</topic><topic>Optics</topic><topic>Original Article</topic><topic>Photonics</topic><topic>Quantum Optics</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Staphylococcus infections</topic><topic>Stem cells</topic><topic>Stem Cells - pathology</topic><topic>Ulcers</topic><topic>Wound Healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moravej, Fahimeh Ghasemi</creatorcontrib><creatorcontrib>Amini, Abdollah</creatorcontrib><creatorcontrib>Masteri Farahani, Reza</creatorcontrib><creatorcontrib>Mohammadi-Yeganeh, Samira</creatorcontrib><creatorcontrib>Mostafavinia, Atarodalsadat</creatorcontrib><creatorcontrib>Ahmadi, Houssein</creatorcontrib><creatorcontrib>Omidi, Hamidreza</creatorcontrib><creatorcontrib>Rezaei, Fatemehalsadat</creatorcontrib><creatorcontrib>Gachkar, Latif</creatorcontrib><creatorcontrib>Hamblin, Michael R</creatorcontrib><creatorcontrib>Chien, Sufan</creatorcontrib><creatorcontrib>Bayat, Mohammad</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Lasers in medical science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moravej, Fahimeh Ghasemi</au><au>Amini, Abdollah</au><au>Masteri Farahani, Reza</au><au>Mohammadi-Yeganeh, Samira</au><au>Mostafavinia, Atarodalsadat</au><au>Ahmadi, Houssein</au><au>Omidi, Hamidreza</au><au>Rezaei, Fatemehalsadat</au><au>Gachkar, Latif</au><au>Hamblin, Michael R</au><au>Chien, Sufan</au><au>Bayat, Mohammad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Photobiomodulation, alone or combined with adipose-derived stem cells, reduces inflammation by modulation of microRNA-146a and interleukin-1ß in a delayed-healing infected wound in diabetic rats</atitle><jtitle>Lasers in medical science</jtitle><stitle>Lasers Med Sci</stitle><addtitle>Lasers Med Sci</addtitle><date>2023-05-27</date><risdate>2023</risdate><volume>38</volume><issue>1</issue><spage>129</spage><epage>129</epage><pages>129-129</pages><artnum>129</artnum><issn>1435-604X</issn><issn>0268-8921</issn><eissn>1435-604X</eissn><abstract>Diabetic wounds are categorized by chronic inflammation, leading to the development of diabetic foot ulcers, which cause amputation and death. Herewith, we examined the effect of photobiomodulation (PBM) plus allogeneic diabetic adipose tissue-derived stem cells (ad-ADS) on stereological parameters and expression levels of interleukin (IL)-1ß and microRNA (miRNA)-146a in the inflammatory (day 4) and proliferation (day 8) stages of wound healing in an ischemic infected (with 2×10
7
colony-forming units of methicillin-resistant
Staphylococcus aureus
) delayed healing wound model (IIDHWM) in type I diabetic (TIDM) rats. There were five groups of rats: group 1 control (C); group 2 (CELL) in which rat wounds received 1×10
6
ad-ADS; group 3 (CL) in which rat wounds received the ad-ADS and were subsequently exposed to PBM(890 nm, 80 Hz, 3.5 J/cm
2
, in vivo); group 4 (CP) in which the ad-ADS preconditioned by the PBM(630 nm + 810 nm, 0.05 W, 1.2 J/cm
2
, 3 times) were implanted into rat wounds; group 5 (CLP) in which the PBM preconditioned ad-ADS were implanted into rat wounds, which were then exposed to PBM. On both days, significantly better histological results were seen in all experimental groups except control. Significantly better histological results were observed in the ad-ADS plus PBM treatment correlated to the ad-ADS alone group (
p
<0.05). Overall, PBM preconditioned ad-ADS followed by PBM of the wound showed the most significant improvement in histological measures correlated to the other experimental groups (
p
<0.05). On days 4 and 8, IL-1 β levels of all experimental groups were lower than the control group; however, on day 8, only the CLP group was different (
p
<0.01). On day 4, miR-146a expression levels were substantially greater in the CLP and CELL groups correlated to the other groups, on day 8 miR-146a in all treatment groups was upper than C (
p
<0.01). ad-ADS plus PBM, ad-ADS, and PBM all improved the inflammatory phase of wound healing in an IIDHWM in TIDM1 rats by reducing inflammatory cells (neutrophils, macrophages) and IL-1ß, and increasing miRNA-146a. The ad-ADS+PBM combination was better than either ad-ADS or PBM alone, because of the higher proliferative and anti-inflammatory effects of the PBM+ad-ADS regimen.</abstract><cop>London</cop><pub>Springer London</pub><pmid>37243832</pmid><doi>10.1007/s10103-023-03786-2</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-8921-4045</orcidid><orcidid>https://orcid.org/0000-0002-5314-5022</orcidid><orcidid>https://orcid.org/0000-0001-6431-4605</orcidid><orcidid>https://orcid.org/0000-0002-9408-3200</orcidid><orcidid>https://orcid.org/0000-0001-6721-2153</orcidid><orcidid>https://orcid.org/0000-0003-2270-5637</orcidid><orcidid>https://orcid.org/0000-0001-8748-1270</orcidid><orcidid>https://orcid.org/0000-0001-5214-4223</orcidid><orcidid>https://orcid.org/0000-0003-3878-3082</orcidid><orcidid>https://orcid.org/0000-0002-1067-4178</orcidid><orcidid>https://orcid.org/0000-0002-6300-4143</orcidid><orcidid>https://orcid.org/0000-0001-5699-9907</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1435-604X |
| ispartof | Lasers in medical science, 2023-05, Vol.38 (1), p.129-129, Article 129 |
| issn | 1435-604X 0268-8921 1435-604X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2820027922 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Adipose tissue Amputation Animals Cytokines Dentistry Diabetes Diabetes mellitus Diabetes Mellitus, Experimental - pathology Drug resistance Inflammation Inflammation - radiotherapy Interleukin 1 Ischemia Lasers Leukocytes (neutrophilic) Light therapy Low-Level Light Therapy - methods Macrophages Medicine Medicine & Public Health Methicillin Methicillin-Resistant Staphylococcus aureus MicroRNAs MicroRNAs - genetics miRNA Optical Devices Optics Original Article Photonics Quantum Optics Rats Rats, Wistar Ribonucleic acid RNA Staphylococcus infections Stem cells Stem Cells - pathology Ulcers Wound Healing |
| title | Photobiomodulation, alone or combined with adipose-derived stem cells, reduces inflammation by modulation of microRNA-146a and interleukin-1ß in a delayed-healing infected wound in diabetic rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T03%3A15%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Photobiomodulation,%20alone%20or%20combined%20with%20adipose-derived%20stem%20cells,%20reduces%20inflammation%20by%20modulation%20of%20microRNA-146a%20and%20interleukin-1%C3%9F%20in%20a%20delayed-healing%20infected%20wound%20in%20diabetic%20rats&rft.jtitle=Lasers%20in%20medical%20science&rft.au=Moravej,%20Fahimeh%20Ghasemi&rft.date=2023-05-27&rft.volume=38&rft.issue=1&rft.spage=129&rft.epage=129&rft.pages=129-129&rft.artnum=129&rft.issn=1435-604X&rft.eissn=1435-604X&rft_id=info:doi/10.1007/s10103-023-03786-2&rft_dat=%3Cproquest_cross%3E2819763499%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2819763499&rft_id=info:pmid/37243832&rfr_iscdi=true |