Effect of Cas9 Protein on the Seed-Target Base Pair of the sgRNA/DNA Hybrid Duplex

CRISPR (clustered regularly interspaced short palindromic repeats)/CRISPR-associated protein (Cas9) has been widely used for gene editing. Not all guide RNAs can cleave the DNA efficiently remains a major challenge to CRISPR/Cas9-mediated genome engineering. Therefore, understanding how the Cas9 com...

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Veröffentlicht in:	The journal of physical chemistry. B 2023-06, Vol.127 (22), p.4989-4997
Hauptverfasser:	Yu, Ting, Liu, Taigang, Wang, Yujie, Zhao, Xuanlin, Zhang, Wenbing
Format:	Artikel
Sprache:	eng
Schlagworte:	B: Biophysical and Biochemical Systems and Processes Base Pairing CRISPR-Associated Protein 9 - genetics CRISPR-Associated Protein 9 - metabolism CRISPR-Cas Systems DNA - chemistry Gene Editing - methods
Online-Zugang:	Volltext
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