Exosomes obtained from adipose mesenchymal stem cells prevent ischemia–reperfusion injury after torsion–detorsion in rat testes
Purpose To investigate the effect of exosomes obtained from adipose-derived mesenchymal stem cells (AD-MSCs) on testicular ischemia–reperfusion (I/R) injury. Methods AD-MSCs from rat adipose tissue were cultured. Characterization of cells was evaluated with CD44, CD90, CD34 and CD45 antibodies. Exos...
Gespeichert in:
| Veröffentlicht in: | Pediatric surgery international 2023-05, Vol.39 (1), p.204-204, Article 204 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 204 |
|---|---|
| container_issue | 1 |
| container_start_page | 204 |
| container_title | Pediatric surgery international |
| container_volume | 39 |
| creator | Şimşek, Fatma Bilgecan Şencan, Aydın Vatansever, H. Seda |
| description | Purpose
To investigate the effect of exosomes obtained from adipose-derived mesenchymal stem cells (AD-MSCs) on testicular ischemia–reperfusion (I/R) injury.
Methods
AD-MSCs from rat adipose tissue were cultured. Characterization of cells was evaluated with CD44, CD90, CD34 and CD45 antibodies. Exosomes from AD-MSCs were obtained with the miRCURY exosome isolation kit. 21 rats were divided into 3 groups. The I/R model was created as 720° torsion for 4 h and reperfusion for 4 h. In the Sham group (SG), only scrotal incision was made. 100 µl of medium in the torsion-control group (T-CG) and 100 µl of exosome in the treatment group (TG) were injected into the testicular parenchyma after detorsion. Johnsen scores of testicles were determined. Apoptosis was evaluated by the TUNEL method.
Results
It was observed that the seminiferous tubule structures were partially disrupted in T-CG, but normal in SG and TG. Johnsen scores in SG, T-CG, and TG were 8.64 ± 0.39, 7.71 ± 0.37, and 8.57 ± 0.39, respectively. Apoptotic cell distribution was 11.28 ± 5.25%, 60.58% ± 1.68% and 17.71 ± 8.34% in SG, T-CG and TG, respectively. In both parameters, the difference between SG and TG was insignificant (
p
> 0.05), the difference between T-CG/TG and SG/T-CG was significant (
p
|
| doi_str_mv | 10.1007/s00383-023-05487-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2818745983</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2818510514</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-93be71c3d6003eac27184d71132139fda86beb576d8cef5722cb9782131e6a8f3</originalsourceid><addsrcrecordid>eNp9kc1KxDAQx4Morq6-gAcJePFSTZptkx5lWT9A8KLnkKZT7bJtatLK7k3wEXxDn8TRrh948BAyZH7zn5n8CTng7IQzJk8DY0KJiMV4komS0XKD7PCJkFGmuNj8FY_IbghzxpgSabZNRkLGcaxkukNeZksXXA2BurwzVQMFLb2rqSmq1gWgmIHGPqxqs6Chg5paWCwCbT08QdPRKtgHqCvz9vzqoQVf9qFyDa2aee9X1JQdeNo5__GISAHrGAHqTUc7QM2wR7ZKswiwv77H5O58dju9jK5vLq6mZ9eRFTLpokzkILkVRYp7g7Gx5GpSSM5FzEVWFkalOeSJTAtloUxwRZtnUmGSQ2pUKcbkeNBtvXvssbWucX7cxzTg-qBjxZWcJJkSiB79Qeeu9w1O90klnCX4uWMSD5T1LgQPpW59VRu_0pzpD4v0YJFGi_SnRXqJRYdr6T6vofgu-fIEATEAAVPNPfif3v_IvgP26aGr</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2818510514</pqid></control><display><type>article</type><title>Exosomes obtained from adipose mesenchymal stem cells prevent ischemia–reperfusion injury after torsion–detorsion in rat testes</title><source>MEDLINE</source><source>SpringerLink (Online service)</source><creator>Şimşek, Fatma Bilgecan ; Şencan, Aydın ; Vatansever, H. Seda</creator><creatorcontrib>Şimşek, Fatma Bilgecan ; Şencan, Aydın ; Vatansever, H. Seda</creatorcontrib><description>Purpose
To investigate the effect of exosomes obtained from adipose-derived mesenchymal stem cells (AD-MSCs) on testicular ischemia–reperfusion (I/R) injury.
Methods
AD-MSCs from rat adipose tissue were cultured. Characterization of cells was evaluated with CD44, CD90, CD34 and CD45 antibodies. Exosomes from AD-MSCs were obtained with the miRCURY exosome isolation kit. 21 rats were divided into 3 groups. The I/R model was created as 720° torsion for 4 h and reperfusion for 4 h. In the Sham group (SG), only scrotal incision was made. 100 µl of medium in the torsion-control group (T-CG) and 100 µl of exosome in the treatment group (TG) were injected into the testicular parenchyma after detorsion. Johnsen scores of testicles were determined. Apoptosis was evaluated by the TUNEL method.
Results
It was observed that the seminiferous tubule structures were partially disrupted in T-CG, but normal in SG and TG. Johnsen scores in SG, T-CG, and TG were 8.64 ± 0.39, 7.71 ± 0.37, and 8.57 ± 0.39, respectively. Apoptotic cell distribution was 11.28 ± 5.25%, 60.58% ± 1.68% and 17.71 ± 8.34% in SG, T-CG and TG, respectively. In both parameters, the difference between SG and TG was insignificant (
p
> 0.05), the difference between T-CG/TG and SG/T-CG was significant (
p
< 0.05).
Conclusion
Exosomes obtained from AD-MSCs are effective in preventing testicular I/R injury. This effect appears to occur because of suppression of apoptotic activity.</description><identifier>ISSN: 1437-9813</identifier><identifier>ISSN: 0179-0358</identifier><identifier>EISSN: 1437-9813</identifier><identifier>DOI: 10.1007/s00383-023-05487-x</identifier><identifier>PMID: 37222876</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Apoptosis ; Exosomes ; Ischemia ; Male ; Medicine ; Medicine & Public Health ; Mesenchymal Stem Cells ; Obesity ; Original Article ; Pediatric Surgery ; Pediatrics ; Rats ; Reperfusion Injury - prevention & control ; Stem cells ; Surgery ; Testis</subject><ispartof>Pediatric surgery international, 2023-05, Vol.39 (1), p.204-204, Article 204</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-93be71c3d6003eac27184d71132139fda86beb576d8cef5722cb9782131e6a8f3</citedby><cites>FETCH-LOGICAL-c375t-93be71c3d6003eac27184d71132139fda86beb576d8cef5722cb9782131e6a8f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00383-023-05487-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00383-023-05487-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37222876$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Şimşek, Fatma Bilgecan</creatorcontrib><creatorcontrib>Şencan, Aydın</creatorcontrib><creatorcontrib>Vatansever, H. Seda</creatorcontrib><title>Exosomes obtained from adipose mesenchymal stem cells prevent ischemia–reperfusion injury after torsion–detorsion in rat testes</title><title>Pediatric surgery international</title><addtitle>Pediatr Surg Int</addtitle><addtitle>Pediatr Surg Int</addtitle><description>Purpose
To investigate the effect of exosomes obtained from adipose-derived mesenchymal stem cells (AD-MSCs) on testicular ischemia–reperfusion (I/R) injury.
Methods
AD-MSCs from rat adipose tissue were cultured. Characterization of cells was evaluated with CD44, CD90, CD34 and CD45 antibodies. Exosomes from AD-MSCs were obtained with the miRCURY exosome isolation kit. 21 rats were divided into 3 groups. The I/R model was created as 720° torsion for 4 h and reperfusion for 4 h. In the Sham group (SG), only scrotal incision was made. 100 µl of medium in the torsion-control group (T-CG) and 100 µl of exosome in the treatment group (TG) were injected into the testicular parenchyma after detorsion. Johnsen scores of testicles were determined. Apoptosis was evaluated by the TUNEL method.
Results
It was observed that the seminiferous tubule structures were partially disrupted in T-CG, but normal in SG and TG. Johnsen scores in SG, T-CG, and TG were 8.64 ± 0.39, 7.71 ± 0.37, and 8.57 ± 0.39, respectively. Apoptotic cell distribution was 11.28 ± 5.25%, 60.58% ± 1.68% and 17.71 ± 8.34% in SG, T-CG and TG, respectively. In both parameters, the difference between SG and TG was insignificant (
p
> 0.05), the difference between T-CG/TG and SG/T-CG was significant (
p
< 0.05).
Conclusion
Exosomes obtained from AD-MSCs are effective in preventing testicular I/R injury. This effect appears to occur because of suppression of apoptotic activity.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Exosomes</subject><subject>Ischemia</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mesenchymal Stem Cells</subject><subject>Obesity</subject><subject>Original Article</subject><subject>Pediatric Surgery</subject><subject>Pediatrics</subject><subject>Rats</subject><subject>Reperfusion Injury - prevention & control</subject><subject>Stem cells</subject><subject>Surgery</subject><subject>Testis</subject><issn>1437-9813</issn><issn>0179-0358</issn><issn>1437-9813</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kc1KxDAQx4Morq6-gAcJePFSTZptkx5lWT9A8KLnkKZT7bJtatLK7k3wEXxDn8TRrh948BAyZH7zn5n8CTng7IQzJk8DY0KJiMV4komS0XKD7PCJkFGmuNj8FY_IbghzxpgSabZNRkLGcaxkukNeZksXXA2BurwzVQMFLb2rqSmq1gWgmIHGPqxqs6Chg5paWCwCbT08QdPRKtgHqCvz9vzqoQVf9qFyDa2aee9X1JQdeNo5__GISAHrGAHqTUc7QM2wR7ZKswiwv77H5O58dju9jK5vLq6mZ9eRFTLpokzkILkVRYp7g7Gx5GpSSM5FzEVWFkalOeSJTAtloUxwRZtnUmGSQ2pUKcbkeNBtvXvssbWucX7cxzTg-qBjxZWcJJkSiB79Qeeu9w1O90klnCX4uWMSD5T1LgQPpW59VRu_0pzpD4v0YJFGi_SnRXqJRYdr6T6vofgu-fIEATEAAVPNPfif3v_IvgP26aGr</recordid><startdate>20230524</startdate><enddate>20230524</enddate><creator>Şimşek, Fatma Bilgecan</creator><creator>Şencan, Aydın</creator><creator>Vatansever, H. Seda</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20230524</creationdate><title>Exosomes obtained from adipose mesenchymal stem cells prevent ischemia–reperfusion injury after torsion–detorsion in rat testes</title><author>Şimşek, Fatma Bilgecan ; Şencan, Aydın ; Vatansever, H. Seda</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-93be71c3d6003eac27184d71132139fda86beb576d8cef5722cb9782131e6a8f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Exosomes</topic><topic>Ischemia</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mesenchymal Stem Cells</topic><topic>Obesity</topic><topic>Original Article</topic><topic>Pediatric Surgery</topic><topic>Pediatrics</topic><topic>Rats</topic><topic>Reperfusion Injury - prevention & control</topic><topic>Stem cells</topic><topic>Surgery</topic><topic>Testis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Şimşek, Fatma Bilgecan</creatorcontrib><creatorcontrib>Şencan, Aydın</creatorcontrib><creatorcontrib>Vatansever, H. Seda</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Consumer Health Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Family Health Database (Proquest)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Pediatric surgery international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Şimşek, Fatma Bilgecan</au><au>Şencan, Aydın</au><au>Vatansever, H. Seda</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exosomes obtained from adipose mesenchymal stem cells prevent ischemia–reperfusion injury after torsion–detorsion in rat testes</atitle><jtitle>Pediatric surgery international</jtitle><stitle>Pediatr Surg Int</stitle><addtitle>Pediatr Surg Int</addtitle><date>2023-05-24</date><risdate>2023</risdate><volume>39</volume><issue>1</issue><spage>204</spage><epage>204</epage><pages>204-204</pages><artnum>204</artnum><issn>1437-9813</issn><issn>0179-0358</issn><eissn>1437-9813</eissn><abstract>Purpose
To investigate the effect of exosomes obtained from adipose-derived mesenchymal stem cells (AD-MSCs) on testicular ischemia–reperfusion (I/R) injury.
Methods
AD-MSCs from rat adipose tissue were cultured. Characterization of cells was evaluated with CD44, CD90, CD34 and CD45 antibodies. Exosomes from AD-MSCs were obtained with the miRCURY exosome isolation kit. 21 rats were divided into 3 groups. The I/R model was created as 720° torsion for 4 h and reperfusion for 4 h. In the Sham group (SG), only scrotal incision was made. 100 µl of medium in the torsion-control group (T-CG) and 100 µl of exosome in the treatment group (TG) were injected into the testicular parenchyma after detorsion. Johnsen scores of testicles were determined. Apoptosis was evaluated by the TUNEL method.
Results
It was observed that the seminiferous tubule structures were partially disrupted in T-CG, but normal in SG and TG. Johnsen scores in SG, T-CG, and TG were 8.64 ± 0.39, 7.71 ± 0.37, and 8.57 ± 0.39, respectively. Apoptotic cell distribution was 11.28 ± 5.25%, 60.58% ± 1.68% and 17.71 ± 8.34% in SG, T-CG and TG, respectively. In both parameters, the difference between SG and TG was insignificant (
p
> 0.05), the difference between T-CG/TG and SG/T-CG was significant (
p
< 0.05).
Conclusion
Exosomes obtained from AD-MSCs are effective in preventing testicular I/R injury. This effect appears to occur because of suppression of apoptotic activity.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>37222876</pmid><doi>10.1007/s00383-023-05487-x</doi><tpages>1</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1437-9813 |
| ispartof | Pediatric surgery international, 2023-05, Vol.39 (1), p.204-204, Article 204 |
| issn | 1437-9813 0179-0358 1437-9813 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2818745983 |
| source | MEDLINE; SpringerLink (Online service) |
| subjects | Animals Apoptosis Exosomes Ischemia Male Medicine Medicine & Public Health Mesenchymal Stem Cells Obesity Original Article Pediatric Surgery Pediatrics Rats Reperfusion Injury - prevention & control Stem cells Surgery Testis |
| title | Exosomes obtained from adipose mesenchymal stem cells prevent ischemia–reperfusion injury after torsion–detorsion in rat testes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T13%3A32%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Exosomes%20obtained%20from%20adipose%20mesenchymal%20stem%20cells%20prevent%20ischemia%E2%80%93reperfusion%20injury%20after%20torsion%E2%80%93detorsion%20in%20rat%20testes&rft.jtitle=Pediatric%20surgery%20international&rft.au=%C5%9Eim%C5%9Fek,%20Fatma%20Bilgecan&rft.date=2023-05-24&rft.volume=39&rft.issue=1&rft.spage=204&rft.epage=204&rft.pages=204-204&rft.artnum=204&rft.issn=1437-9813&rft.eissn=1437-9813&rft_id=info:doi/10.1007/s00383-023-05487-x&rft_dat=%3Cproquest_cross%3E2818510514%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2818510514&rft_id=info:pmid/37222876&rfr_iscdi=true |