Integrated mass spectrometry imaging and single‐cell transcriptome atlas strategies provide novel insights into taxoid biosynthesis and transport in Taxus mairei stems

SUMMARY Taxol, which is a widely used important chemotherapeutic agent, was originally isolated from Taxus stem barks. However, little is known about the precise distribution of taxoids and the transcriptional regulation of taxoid biosynthesis across Taxus stems. Here, we used MALDI‐IMS analysis to...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The Plant journal : for cell and molecular biology 2023-09, Vol.115 (5), p.1243-1260
Hauptverfasser: Yu, Chunna, Hou, Kailin, Zhang, Hongshan, Liang, Xueshuang, Chen, Cheng, Wang, Zhijing, Wu, Qicong, Chen, Ganlin, He, Jiaxu, Bai, Enhui, Li, Xinfen, Du, Tingrui, Wang, Yifan, Wang, Mingshuang, Feng, Shangguo, Wang, Huizhong, Shen, Chenjia
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 1260
container_issue 5
container_start_page 1243
container_title The Plant journal : for cell and molecular biology
container_volume 115
creator Yu, Chunna
Hou, Kailin
Zhang, Hongshan
Liang, Xueshuang
Chen, Cheng
Wang, Zhijing
Wu, Qicong
Chen, Ganlin
He, Jiaxu
Bai, Enhui
Li, Xinfen
Du, Tingrui
Wang, Yifan
Wang, Mingshuang
Feng, Shangguo
Wang, Huizhong
Shen, Chenjia
description SUMMARY Taxol, which is a widely used important chemotherapeutic agent, was originally isolated from Taxus stem barks. However, little is known about the precise distribution of taxoids and the transcriptional regulation of taxoid biosynthesis across Taxus stems. Here, we used MALDI‐IMS analysis to visualize the taxoid distribution across Taxus mairei stems and single‐cell RNA sequencing to generate expression profiles. A single‐cell T. mairei stem atlas was created, providing a spatial distribution pattern of Taxus stem cells. Cells were reordered using a main developmental pseudotime trajectory which provided temporal distribution patterns in Taxus stem cells. Most known taxol biosynthesis‐related genes were primarily expressed in epidermal, endodermal, and xylem parenchyma cells, which caused an uneven taxoid distribution across T. mairei stems. We developed a single‐cell strategy to screen novel transcription factors (TFs) involved in taxol biosynthesis regulation. Several TF genes, such as endodermal cell‐specific MYB47 and xylem parenchyma cell‐specific NAC2 and bHLH68, were implicated as potential regulators of taxol biosynthesis. Furthermore, an ATP‐binding cassette family transporter gene, ABCG2, was proposed as a potential taxoid transporter candidate. In summary, we generated a single‐cell Taxus stem metabolic atlas and identified molecular mechanisms underpinning the cell‐specific transcriptional regulation of the taxol biosynthesis pathway. Significance Statement Integrating mass spectrometry imaging and single‐cell transcriptome atlas strategies, we created a single‐cell T. mairei stem atlas and provided a spatial distribution pattern of Taxus stem cells. Screening for novel transcription factors helped us to reveal the molecular mechanisms underpinning the cell‐specific transcriptional regulation of the taxol biosynthesis pathway.
doi_str_mv 10.1111/tpj.16315
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2818060621</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2818060621</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3535-2a5d4825a81102bb42d1cd85f99a37d8eb6e1c91c251950a927290351bd87953</originalsourceid><addsrcrecordid>eNp1kb1uFDEQx60IlByBghdAlmhCsYk_zvtRoohAUCQorqBbeddzG59214vHG3JdHiGvwWvxJMzdBQok3HiKn3-emT9jr6U4l3Qu0rQ5l7mW5ogtpM5NpqX-9owtRJWLrFhKdcJeIG6EkIXOl8fsRBdKVgQu2M_rMUEXbQLHB4vIcYI2xTBAilvuB9v5seN2dByp6OHXw2MLfc9TtCO20U-JUG5Tb-lp2nk6D8inGO68Az6GO-i5H9F3twmpSIEnex-8440PuB3TLaDH_Qd75RRiIoyv7P2M1JGP4EkMA75kz9e2R3j1dJ-y1dWH1eWn7ObLx-vL9zdZq402mbLGLUtlbCmlUE2zVE62rjTrqrK6cCU0Oci2kq0ysjLCVqpQldBGNq4sKqNP2dlBSyN8nwFTPXjcjWxHCDPWqpSlyEWuJKFv_0E3YY4jNUeUKciry4KodweqjQExwrqeIu01bmsp6l18NcVX7-Mj9s2TcW4GcH_JP3kRcHEAfvgetv831auvnw_K38UTqSk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2857729387</pqid></control><display><type>article</type><title>Integrated mass spectrometry imaging and single‐cell transcriptome atlas strategies provide novel insights into taxoid biosynthesis and transport in Taxus mairei stems</title><source>Access via Wiley Online Library</source><source>IngentaConnect Free/Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library (Open Access Collection)</source><creator>Yu, Chunna ; Hou, Kailin ; Zhang, Hongshan ; Liang, Xueshuang ; Chen, Cheng ; Wang, Zhijing ; Wu, Qicong ; Chen, Ganlin ; He, Jiaxu ; Bai, Enhui ; Li, Xinfen ; Du, Tingrui ; Wang, Yifan ; Wang, Mingshuang ; Feng, Shangguo ; Wang, Huizhong ; Shen, Chenjia</creator><creatorcontrib>Yu, Chunna ; Hou, Kailin ; Zhang, Hongshan ; Liang, Xueshuang ; Chen, Cheng ; Wang, Zhijing ; Wu, Qicong ; Chen, Ganlin ; He, Jiaxu ; Bai, Enhui ; Li, Xinfen ; Du, Tingrui ; Wang, Yifan ; Wang, Mingshuang ; Feng, Shangguo ; Wang, Huizhong ; Shen, Chenjia</creatorcontrib><description>SUMMARY Taxol, which is a widely used important chemotherapeutic agent, was originally isolated from Taxus stem barks. However, little is known about the precise distribution of taxoids and the transcriptional regulation of taxoid biosynthesis across Taxus stems. Here, we used MALDI‐IMS analysis to visualize the taxoid distribution across Taxus mairei stems and single‐cell RNA sequencing to generate expression profiles. A single‐cell T. mairei stem atlas was created, providing a spatial distribution pattern of Taxus stem cells. Cells were reordered using a main developmental pseudotime trajectory which provided temporal distribution patterns in Taxus stem cells. Most known taxol biosynthesis‐related genes were primarily expressed in epidermal, endodermal, and xylem parenchyma cells, which caused an uneven taxoid distribution across T. mairei stems. We developed a single‐cell strategy to screen novel transcription factors (TFs) involved in taxol biosynthesis regulation. Several TF genes, such as endodermal cell‐specific MYB47 and xylem parenchyma cell‐specific NAC2 and bHLH68, were implicated as potential regulators of taxol biosynthesis. Furthermore, an ATP‐binding cassette family transporter gene, ABCG2, was proposed as a potential taxoid transporter candidate. In summary, we generated a single‐cell Taxus stem metabolic atlas and identified molecular mechanisms underpinning the cell‐specific transcriptional regulation of the taxol biosynthesis pathway. Significance Statement Integrating mass spectrometry imaging and single‐cell transcriptome atlas strategies, we created a single‐cell T. mairei stem atlas and provided a spatial distribution pattern of Taxus stem cells. Screening for novel transcription factors helped us to reveal the molecular mechanisms underpinning the cell‐specific transcriptional regulation of the taxol biosynthesis pathway.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1111/tpj.16315</identifier><identifier>PMID: 37219365</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Biosynthesis ; Distribution patterns ; Gene expression ; Gene regulation ; Gene sequencing ; Genes ; Mass spectrometry ; Mass spectroscopy ; Matrix‐assisted laser desorption/ionization imaging mass spectrometry analysis ; Molecular modelling ; Paclitaxel ; Parenchyma ; scRNA‐seq ; Spatial distribution ; Stem cells ; Taxol ; taxol biosynthesis ; Taxus mairei ; Taxus stem ; Temporal distribution ; Transcription factors ; transcription regulation ; Transcriptomes ; transporter ; Xylem</subject><ispartof>The Plant journal : for cell and molecular biology, 2023-09, Vol.115 (5), p.1243-1260</ispartof><rights>2023 Society for Experimental Biology and John Wiley &amp; Sons Ltd.</rights><rights>Copyright © 2023 Society for Experimental Biology and John Wiley &amp; Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3535-2a5d4825a81102bb42d1cd85f99a37d8eb6e1c91c251950a927290351bd87953</citedby><cites>FETCH-LOGICAL-c3535-2a5d4825a81102bb42d1cd85f99a37d8eb6e1c91c251950a927290351bd87953</cites><orcidid>0000-0002-8575-0593</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Ftpj.16315$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Ftpj.16315$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37219365$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yu, Chunna</creatorcontrib><creatorcontrib>Hou, Kailin</creatorcontrib><creatorcontrib>Zhang, Hongshan</creatorcontrib><creatorcontrib>Liang, Xueshuang</creatorcontrib><creatorcontrib>Chen, Cheng</creatorcontrib><creatorcontrib>Wang, Zhijing</creatorcontrib><creatorcontrib>Wu, Qicong</creatorcontrib><creatorcontrib>Chen, Ganlin</creatorcontrib><creatorcontrib>He, Jiaxu</creatorcontrib><creatorcontrib>Bai, Enhui</creatorcontrib><creatorcontrib>Li, Xinfen</creatorcontrib><creatorcontrib>Du, Tingrui</creatorcontrib><creatorcontrib>Wang, Yifan</creatorcontrib><creatorcontrib>Wang, Mingshuang</creatorcontrib><creatorcontrib>Feng, Shangguo</creatorcontrib><creatorcontrib>Wang, Huizhong</creatorcontrib><creatorcontrib>Shen, Chenjia</creatorcontrib><title>Integrated mass spectrometry imaging and single‐cell transcriptome atlas strategies provide novel insights into taxoid biosynthesis and transport in Taxus mairei stems</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>SUMMARY Taxol, which is a widely used important chemotherapeutic agent, was originally isolated from Taxus stem barks. However, little is known about the precise distribution of taxoids and the transcriptional regulation of taxoid biosynthesis across Taxus stems. Here, we used MALDI‐IMS analysis to visualize the taxoid distribution across Taxus mairei stems and single‐cell RNA sequencing to generate expression profiles. A single‐cell T. mairei stem atlas was created, providing a spatial distribution pattern of Taxus stem cells. Cells were reordered using a main developmental pseudotime trajectory which provided temporal distribution patterns in Taxus stem cells. Most known taxol biosynthesis‐related genes were primarily expressed in epidermal, endodermal, and xylem parenchyma cells, which caused an uneven taxoid distribution across T. mairei stems. We developed a single‐cell strategy to screen novel transcription factors (TFs) involved in taxol biosynthesis regulation. Several TF genes, such as endodermal cell‐specific MYB47 and xylem parenchyma cell‐specific NAC2 and bHLH68, were implicated as potential regulators of taxol biosynthesis. Furthermore, an ATP‐binding cassette family transporter gene, ABCG2, was proposed as a potential taxoid transporter candidate. In summary, we generated a single‐cell Taxus stem metabolic atlas and identified molecular mechanisms underpinning the cell‐specific transcriptional regulation of the taxol biosynthesis pathway. Significance Statement Integrating mass spectrometry imaging and single‐cell transcriptome atlas strategies, we created a single‐cell T. mairei stem atlas and provided a spatial distribution pattern of Taxus stem cells. Screening for novel transcription factors helped us to reveal the molecular mechanisms underpinning the cell‐specific transcriptional regulation of the taxol biosynthesis pathway.</description><subject>Biosynthesis</subject><subject>Distribution patterns</subject><subject>Gene expression</subject><subject>Gene regulation</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Matrix‐assisted laser desorption/ionization imaging mass spectrometry analysis</subject><subject>Molecular modelling</subject><subject>Paclitaxel</subject><subject>Parenchyma</subject><subject>scRNA‐seq</subject><subject>Spatial distribution</subject><subject>Stem cells</subject><subject>Taxol</subject><subject>taxol biosynthesis</subject><subject>Taxus mairei</subject><subject>Taxus stem</subject><subject>Temporal distribution</subject><subject>Transcription factors</subject><subject>transcription regulation</subject><subject>Transcriptomes</subject><subject>transporter</subject><subject>Xylem</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp1kb1uFDEQx60IlByBghdAlmhCsYk_zvtRoohAUCQorqBbeddzG59214vHG3JdHiGvwWvxJMzdBQok3HiKn3-emT9jr6U4l3Qu0rQ5l7mW5ogtpM5NpqX-9owtRJWLrFhKdcJeIG6EkIXOl8fsRBdKVgQu2M_rMUEXbQLHB4vIcYI2xTBAilvuB9v5seN2dByp6OHXw2MLfc9TtCO20U-JUG5Tb-lp2nk6D8inGO68Az6GO-i5H9F3twmpSIEnex-8440PuB3TLaDH_Qd75RRiIoyv7P2M1JGP4EkMA75kz9e2R3j1dJ-y1dWH1eWn7ObLx-vL9zdZq402mbLGLUtlbCmlUE2zVE62rjTrqrK6cCU0Oci2kq0ysjLCVqpQldBGNq4sKqNP2dlBSyN8nwFTPXjcjWxHCDPWqpSlyEWuJKFv_0E3YY4jNUeUKciry4KodweqjQExwrqeIu01bmsp6l18NcVX7-Mj9s2TcW4GcH_JP3kRcHEAfvgetv831auvnw_K38UTqSk</recordid><startdate>202309</startdate><enddate>202309</enddate><creator>Yu, Chunna</creator><creator>Hou, Kailin</creator><creator>Zhang, Hongshan</creator><creator>Liang, Xueshuang</creator><creator>Chen, Cheng</creator><creator>Wang, Zhijing</creator><creator>Wu, Qicong</creator><creator>Chen, Ganlin</creator><creator>He, Jiaxu</creator><creator>Bai, Enhui</creator><creator>Li, Xinfen</creator><creator>Du, Tingrui</creator><creator>Wang, Yifan</creator><creator>Wang, Mingshuang</creator><creator>Feng, Shangguo</creator><creator>Wang, Huizhong</creator><creator>Shen, Chenjia</creator><general>Blackwell Publishing Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8575-0593</orcidid></search><sort><creationdate>202309</creationdate><title>Integrated mass spectrometry imaging and single‐cell transcriptome atlas strategies provide novel insights into taxoid biosynthesis and transport in Taxus mairei stems</title><author>Yu, Chunna ; Hou, Kailin ; Zhang, Hongshan ; Liang, Xueshuang ; Chen, Cheng ; Wang, Zhijing ; Wu, Qicong ; Chen, Ganlin ; He, Jiaxu ; Bai, Enhui ; Li, Xinfen ; Du, Tingrui ; Wang, Yifan ; Wang, Mingshuang ; Feng, Shangguo ; Wang, Huizhong ; Shen, Chenjia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3535-2a5d4825a81102bb42d1cd85f99a37d8eb6e1c91c251950a927290351bd87953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Biosynthesis</topic><topic>Distribution patterns</topic><topic>Gene expression</topic><topic>Gene regulation</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Matrix‐assisted laser desorption/ionization imaging mass spectrometry analysis</topic><topic>Molecular modelling</topic><topic>Paclitaxel</topic><topic>Parenchyma</topic><topic>scRNA‐seq</topic><topic>Spatial distribution</topic><topic>Stem cells</topic><topic>Taxol</topic><topic>taxol biosynthesis</topic><topic>Taxus mairei</topic><topic>Taxus stem</topic><topic>Temporal distribution</topic><topic>Transcription factors</topic><topic>transcription regulation</topic><topic>Transcriptomes</topic><topic>transporter</topic><topic>Xylem</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yu, Chunna</creatorcontrib><creatorcontrib>Hou, Kailin</creatorcontrib><creatorcontrib>Zhang, Hongshan</creatorcontrib><creatorcontrib>Liang, Xueshuang</creatorcontrib><creatorcontrib>Chen, Cheng</creatorcontrib><creatorcontrib>Wang, Zhijing</creatorcontrib><creatorcontrib>Wu, Qicong</creatorcontrib><creatorcontrib>Chen, Ganlin</creatorcontrib><creatorcontrib>He, Jiaxu</creatorcontrib><creatorcontrib>Bai, Enhui</creatorcontrib><creatorcontrib>Li, Xinfen</creatorcontrib><creatorcontrib>Du, Tingrui</creatorcontrib><creatorcontrib>Wang, Yifan</creatorcontrib><creatorcontrib>Wang, Mingshuang</creatorcontrib><creatorcontrib>Feng, Shangguo</creatorcontrib><creatorcontrib>Wang, Huizhong</creatorcontrib><creatorcontrib>Shen, Chenjia</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yu, Chunna</au><au>Hou, Kailin</au><au>Zhang, Hongshan</au><au>Liang, Xueshuang</au><au>Chen, Cheng</au><au>Wang, Zhijing</au><au>Wu, Qicong</au><au>Chen, Ganlin</au><au>He, Jiaxu</au><au>Bai, Enhui</au><au>Li, Xinfen</au><au>Du, Tingrui</au><au>Wang, Yifan</au><au>Wang, Mingshuang</au><au>Feng, Shangguo</au><au>Wang, Huizhong</au><au>Shen, Chenjia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Integrated mass spectrometry imaging and single‐cell transcriptome atlas strategies provide novel insights into taxoid biosynthesis and transport in Taxus mairei stems</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2023-09</date><risdate>2023</risdate><volume>115</volume><issue>5</issue><spage>1243</spage><epage>1260</epage><pages>1243-1260</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>SUMMARY Taxol, which is a widely used important chemotherapeutic agent, was originally isolated from Taxus stem barks. However, little is known about the precise distribution of taxoids and the transcriptional regulation of taxoid biosynthesis across Taxus stems. Here, we used MALDI‐IMS analysis to visualize the taxoid distribution across Taxus mairei stems and single‐cell RNA sequencing to generate expression profiles. A single‐cell T. mairei stem atlas was created, providing a spatial distribution pattern of Taxus stem cells. Cells were reordered using a main developmental pseudotime trajectory which provided temporal distribution patterns in Taxus stem cells. Most known taxol biosynthesis‐related genes were primarily expressed in epidermal, endodermal, and xylem parenchyma cells, which caused an uneven taxoid distribution across T. mairei stems. We developed a single‐cell strategy to screen novel transcription factors (TFs) involved in taxol biosynthesis regulation. Several TF genes, such as endodermal cell‐specific MYB47 and xylem parenchyma cell‐specific NAC2 and bHLH68, were implicated as potential regulators of taxol biosynthesis. Furthermore, an ATP‐binding cassette family transporter gene, ABCG2, was proposed as a potential taxoid transporter candidate. In summary, we generated a single‐cell Taxus stem metabolic atlas and identified molecular mechanisms underpinning the cell‐specific transcriptional regulation of the taxol biosynthesis pathway. Significance Statement Integrating mass spectrometry imaging and single‐cell transcriptome atlas strategies, we created a single‐cell T. mairei stem atlas and provided a spatial distribution pattern of Taxus stem cells. Screening for novel transcription factors helped us to reveal the molecular mechanisms underpinning the cell‐specific transcriptional regulation of the taxol biosynthesis pathway.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>37219365</pmid><doi>10.1111/tpj.16315</doi><tpages>1260</tpages><orcidid>https://orcid.org/0000-0002-8575-0593</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0960-7412
ispartof The Plant journal : for cell and molecular biology, 2023-09, Vol.115 (5), p.1243-1260
issn 0960-7412
1365-313X
language eng
recordid cdi_proquest_miscellaneous_2818060621
source Access via Wiley Online Library; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection)
subjects Biosynthesis
Distribution patterns
Gene expression
Gene regulation
Gene sequencing
Genes
Mass spectrometry
Mass spectroscopy
Matrix‐assisted laser desorption/ionization imaging mass spectrometry analysis
Molecular modelling
Paclitaxel
Parenchyma
scRNA‐seq
Spatial distribution
Stem cells
Taxol
taxol biosynthesis
Taxus mairei
Taxus stem
Temporal distribution
Transcription factors
transcription regulation
Transcriptomes
transporter
Xylem
title Integrated mass spectrometry imaging and single‐cell transcriptome atlas strategies provide novel insights into taxoid biosynthesis and transport in Taxus mairei stems
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T12%3A38%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Integrated%20mass%20spectrometry%20imaging%20and%20single%E2%80%90cell%20transcriptome%20atlas%20strategies%20provide%20novel%20insights%20into%20taxoid%20biosynthesis%20and%20transport%20in%20Taxus%20mairei%20stems&rft.jtitle=The%20Plant%20journal%20:%20for%20cell%20and%20molecular%20biology&rft.au=Yu,%20Chunna&rft.date=2023-09&rft.volume=115&rft.issue=5&rft.spage=1243&rft.epage=1260&rft.pages=1243-1260&rft.issn=0960-7412&rft.eissn=1365-313X&rft_id=info:doi/10.1111/tpj.16315&rft_dat=%3Cproquest_cross%3E2818060621%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2857729387&rft_id=info:pmid/37219365&rfr_iscdi=true