miR‐27a‐5p alleviates periodontal inflammation by targeting phosphatase and tensin homolog deleted on chromosome ten
Introduction MicroRNAs (miRNAs), a type of non‐coding RNA, have been demonstrated to be essential posttranscriptional modulators in oral diseases and inflammatory responses. However, the specific role of miR‐27a‐5p in periodontitis requires further investigation. In this study, we used both cellular...
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Veröffentlicht in: | Molecular oral microbiology 2023-08, Vol.38 (4), p.309-320 |
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description | Introduction
MicroRNAs (miRNAs), a type of non‐coding RNA, have been demonstrated to be essential posttranscriptional modulators in oral diseases and inflammatory responses. However, the specific role of miR‐27a‐5p in periodontitis requires further investigation. In this study, we used both cellular and animal models to determine how miR‐27a‐5p affects the pathogenesis of periodontitis and its associated biological functions.
Methods
Quantitative real‐time polymerase chain reaction and western blotting were used to analyze the expression of cytokines, phosphatase and tensin homolog deleted on chromosome ten (PTEN), and miR‐27a‐5p transcription. Investigation of alveolar bone resorption and inflammation of the periodontium in ligature‐induced periodontitis in mice was performed using micro‐computed tomography (micro‐CT), hematoxylin‐eosin (HE) staining, and tartrate‐resistant acid phosphatase (TRAP) staining. The binding of miR‐27a‐5p and PTEN was predicted using the TargetScan database and experimentally confirmed using dual luciferase reporter gene assays.
Results
The inflamed gingiva showed lower levels of miR‐27a‐5p. Macrophages from miR‐27a‐5p–/– mice produced much higher quantities of pro‐inflammatory cytokines owing to the stimulation of Porphyromonas gingivalis lipopolysaccharide, and miR‐27a‐5p–/– mice with ligature‐induced periodontitis also exhibited more severe alveolar bone resorption and damage to the periodontium. Target validation assays identified PTEN as a direct target of bona. Blocking PTEN expression partially reduced inflammation, both in vitro and in vivo.
Conclusions
miR‐27a‐5p alleviated the inflammatory response in periodontitis by targeting PTEN.
This work declares that miR‐27a‐5p alleviates periodontal inflammation by targeting phosphatase and tensin homolog deleted on chromosome ten in ligatured‐induced periodontitis mice and macrophages stimulated with Porphyromonas gingivalis lipopolysaccharide, which contributes to further exploration in the pathogenesis of periodontitis. |
doi_str_mv | 10.1111/omi.12416 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2818055027</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2835999117</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2436-80dde43ca85acc9723f1891dae50926922f1f19acfd39038c0a07afef5efa1e43</originalsourceid><addsrcrecordid>eNp10c1qVDEUB_Agii21C19AAm50MW2S-5GbpRQ_CpWC6PpympzMpOTjmmSqs_MRfEafxIxTuxA8iySE3_kTcgh5ztkZb3WegjvjoufjI3IsWM9XnPH-8cOZjUfktJRb1qrjvZTyKTnqpODjOMhj8j24T79-_BQS2josFLzHOwcVC10wu2RSrOCpi9ZDCFBdivRmRyvkNVYX13TZpLJsoEJBCtHQirG4SDcpJJ_W1KDHioa2Nr3J7bKkgHv0jDyx4Aue3u8n5Mu7t58vPqyurt9fXry5WmnRd-NqYsZg32mYBtBaSdFZPiluAAemxKiEsNxyBdqaTrFu0gyYBIt2QAu8dZ6QV4fcJaevWyx1Dq5o9B4ipm2ZxcQnNgxMyEZf_kNv0zbH9rqmukEpxflevT4onVMpGe28ZBcg72bO5v1E5jaR-c9Emn1xn7i9CWge5N__b-D8AL45j7v_J83XHy8Pkb8B2mGYGg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2835999117</pqid></control><display><type>article</type><title>miR‐27a‐5p alleviates periodontal inflammation by targeting phosphatase and tensin homolog deleted on chromosome ten</title><source>Wiley-Blackwell Journals</source><source>MEDLINE</source><creator>Deng, Li ; Huo, Peng‐cheng ; Feng, Mei‐ting ; Wang, Rui‐ling ; Jing, Rui ; Luo, Li‐jun</creator><creatorcontrib>Deng, Li ; Huo, Peng‐cheng ; Feng, Mei‐ting ; Wang, Rui‐ling ; Jing, Rui ; Luo, Li‐jun</creatorcontrib><description>Introduction
MicroRNAs (miRNAs), a type of non‐coding RNA, have been demonstrated to be essential posttranscriptional modulators in oral diseases and inflammatory responses. However, the specific role of miR‐27a‐5p in periodontitis requires further investigation. In this study, we used both cellular and animal models to determine how miR‐27a‐5p affects the pathogenesis of periodontitis and its associated biological functions.
Methods
Quantitative real‐time polymerase chain reaction and western blotting were used to analyze the expression of cytokines, phosphatase and tensin homolog deleted on chromosome ten (PTEN), and miR‐27a‐5p transcription. Investigation of alveolar bone resorption and inflammation of the periodontium in ligature‐induced periodontitis in mice was performed using micro‐computed tomography (micro‐CT), hematoxylin‐eosin (HE) staining, and tartrate‐resistant acid phosphatase (TRAP) staining. The binding of miR‐27a‐5p and PTEN was predicted using the TargetScan database and experimentally confirmed using dual luciferase reporter gene assays.
Results
The inflamed gingiva showed lower levels of miR‐27a‐5p. Macrophages from miR‐27a‐5p–/– mice produced much higher quantities of pro‐inflammatory cytokines owing to the stimulation of Porphyromonas gingivalis lipopolysaccharide, and miR‐27a‐5p–/– mice with ligature‐induced periodontitis also exhibited more severe alveolar bone resorption and damage to the periodontium. Target validation assays identified PTEN as a direct target of bona. Blocking PTEN expression partially reduced inflammation, both in vitro and in vivo.
Conclusions
miR‐27a‐5p alleviated the inflammatory response in periodontitis by targeting PTEN.
This work declares that miR‐27a‐5p alleviates periodontal inflammation by targeting phosphatase and tensin homolog deleted on chromosome ten in ligatured‐induced periodontitis mice and macrophages stimulated with Porphyromonas gingivalis lipopolysaccharide, which contributes to further exploration in the pathogenesis of periodontitis.</description><identifier>ISSN: 2041-1006</identifier><identifier>EISSN: 2041-1014</identifier><identifier>DOI: 10.1111/omi.12416</identifier><identifier>PMID: 37216657</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Acid phosphatase ; Acid phosphatase (tartrate-resistant) ; Acid resistance ; Alveolar bone ; alveolar bone loss ; Animal models ; Animals ; Bone resorption ; Bone Resorption - genetics ; Chromosomes ; Chromosomes - metabolism ; Computed tomography ; Cytokines ; Cytokines - genetics ; Gum disease ; Homology ; Inflammation ; Inflammatory response ; Lipopolysaccharides ; Macrophages ; Mice ; MicroRNAs ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miRNA ; miR‐27a‐5p ; Modulators ; Pathogenesis ; Periodontitis ; Periodontitis - genetics ; Periodontium ; Phosphatase ; Polymerase chain reaction ; Post-transcription ; PTEN ; PTEN Phosphohydrolase - genetics ; PTEN Phosphohydrolase - metabolism ; PTEN protein ; Reporter gene ; Staining ; Tensins - genetics ; Western blotting ; X-Ray Microtomography</subject><ispartof>Molecular oral microbiology, 2023-08, Vol.38 (4), p.309-320</ispartof><rights>2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2436-80dde43ca85acc9723f1891dae50926922f1f19acfd39038c0a07afef5efa1e43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fomi.12416$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fomi.12416$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37216657$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deng, Li</creatorcontrib><creatorcontrib>Huo, Peng‐cheng</creatorcontrib><creatorcontrib>Feng, Mei‐ting</creatorcontrib><creatorcontrib>Wang, Rui‐ling</creatorcontrib><creatorcontrib>Jing, Rui</creatorcontrib><creatorcontrib>Luo, Li‐jun</creatorcontrib><title>miR‐27a‐5p alleviates periodontal inflammation by targeting phosphatase and tensin homolog deleted on chromosome ten</title><title>Molecular oral microbiology</title><addtitle>Mol Oral Microbiol</addtitle><description>Introduction
MicroRNAs (miRNAs), a type of non‐coding RNA, have been demonstrated to be essential posttranscriptional modulators in oral diseases and inflammatory responses. However, the specific role of miR‐27a‐5p in periodontitis requires further investigation. In this study, we used both cellular and animal models to determine how miR‐27a‐5p affects the pathogenesis of periodontitis and its associated biological functions.
Methods
Quantitative real‐time polymerase chain reaction and western blotting were used to analyze the expression of cytokines, phosphatase and tensin homolog deleted on chromosome ten (PTEN), and miR‐27a‐5p transcription. Investigation of alveolar bone resorption and inflammation of the periodontium in ligature‐induced periodontitis in mice was performed using micro‐computed tomography (micro‐CT), hematoxylin‐eosin (HE) staining, and tartrate‐resistant acid phosphatase (TRAP) staining. The binding of miR‐27a‐5p and PTEN was predicted using the TargetScan database and experimentally confirmed using dual luciferase reporter gene assays.
Results
The inflamed gingiva showed lower levels of miR‐27a‐5p. Macrophages from miR‐27a‐5p–/– mice produced much higher quantities of pro‐inflammatory cytokines owing to the stimulation of Porphyromonas gingivalis lipopolysaccharide, and miR‐27a‐5p–/– mice with ligature‐induced periodontitis also exhibited more severe alveolar bone resorption and damage to the periodontium. Target validation assays identified PTEN as a direct target of bona. Blocking PTEN expression partially reduced inflammation, both in vitro and in vivo.
Conclusions
miR‐27a‐5p alleviated the inflammatory response in periodontitis by targeting PTEN.
This work declares that miR‐27a‐5p alleviates periodontal inflammation by targeting phosphatase and tensin homolog deleted on chromosome ten in ligatured‐induced periodontitis mice and macrophages stimulated with Porphyromonas gingivalis lipopolysaccharide, which contributes to further exploration in the pathogenesis of periodontitis.</description><subject>Acid phosphatase</subject><subject>Acid phosphatase (tartrate-resistant)</subject><subject>Acid resistance</subject><subject>Alveolar bone</subject><subject>alveolar bone loss</subject><subject>Animal models</subject><subject>Animals</subject><subject>Bone resorption</subject><subject>Bone Resorption - genetics</subject><subject>Chromosomes</subject><subject>Chromosomes - metabolism</subject><subject>Computed tomography</subject><subject>Cytokines</subject><subject>Cytokines - genetics</subject><subject>Gum disease</subject><subject>Homology</subject><subject>Inflammation</subject><subject>Inflammatory response</subject><subject>Lipopolysaccharides</subject><subject>Macrophages</subject><subject>Mice</subject><subject>MicroRNAs</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miRNA</subject><subject>miR‐27a‐5p</subject><subject>Modulators</subject><subject>Pathogenesis</subject><subject>Periodontitis</subject><subject>Periodontitis - genetics</subject><subject>Periodontium</subject><subject>Phosphatase</subject><subject>Polymerase chain reaction</subject><subject>Post-transcription</subject><subject>PTEN</subject><subject>PTEN Phosphohydrolase - genetics</subject><subject>PTEN Phosphohydrolase - metabolism</subject><subject>PTEN protein</subject><subject>Reporter gene</subject><subject>Staining</subject><subject>Tensins - genetics</subject><subject>Western blotting</subject><subject>X-Ray Microtomography</subject><issn>2041-1006</issn><issn>2041-1014</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10c1qVDEUB_Agii21C19AAm50MW2S-5GbpRQ_CpWC6PpympzMpOTjmmSqs_MRfEafxIxTuxA8iySE3_kTcgh5ztkZb3WegjvjoufjI3IsWM9XnPH-8cOZjUfktJRb1qrjvZTyKTnqpODjOMhj8j24T79-_BQS2josFLzHOwcVC10wu2RSrOCpi9ZDCFBdivRmRyvkNVYX13TZpLJsoEJBCtHQirG4SDcpJJ_W1KDHioa2Nr3J7bKkgHv0jDyx4Aue3u8n5Mu7t58vPqyurt9fXry5WmnRd-NqYsZg32mYBtBaSdFZPiluAAemxKiEsNxyBdqaTrFu0gyYBIt2QAu8dZ6QV4fcJaevWyx1Dq5o9B4ipm2ZxcQnNgxMyEZf_kNv0zbH9rqmukEpxflevT4onVMpGe28ZBcg72bO5v1E5jaR-c9Emn1xn7i9CWge5N__b-D8AL45j7v_J83XHy8Pkb8B2mGYGg</recordid><startdate>202308</startdate><enddate>202308</enddate><creator>Deng, Li</creator><creator>Huo, Peng‐cheng</creator><creator>Feng, Mei‐ting</creator><creator>Wang, Rui‐ling</creator><creator>Jing, Rui</creator><creator>Luo, Li‐jun</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>202308</creationdate><title>miR‐27a‐5p alleviates periodontal inflammation by targeting phosphatase and tensin homolog deleted on chromosome ten</title><author>Deng, Li ; Huo, Peng‐cheng ; Feng, Mei‐ting ; Wang, Rui‐ling ; Jing, Rui ; Luo, Li‐jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2436-80dde43ca85acc9723f1891dae50926922f1f19acfd39038c0a07afef5efa1e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Acid phosphatase</topic><topic>Acid phosphatase (tartrate-resistant)</topic><topic>Acid resistance</topic><topic>Alveolar bone</topic><topic>alveolar bone loss</topic><topic>Animal models</topic><topic>Animals</topic><topic>Bone resorption</topic><topic>Bone Resorption - genetics</topic><topic>Chromosomes</topic><topic>Chromosomes - metabolism</topic><topic>Computed tomography</topic><topic>Cytokines</topic><topic>Cytokines - genetics</topic><topic>Gum disease</topic><topic>Homology</topic><topic>Inflammation</topic><topic>Inflammatory response</topic><topic>Lipopolysaccharides</topic><topic>Macrophages</topic><topic>Mice</topic><topic>MicroRNAs</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miRNA</topic><topic>miR‐27a‐5p</topic><topic>Modulators</topic><topic>Pathogenesis</topic><topic>Periodontitis</topic><topic>Periodontitis - genetics</topic><topic>Periodontium</topic><topic>Phosphatase</topic><topic>Polymerase chain reaction</topic><topic>Post-transcription</topic><topic>PTEN</topic><topic>PTEN Phosphohydrolase - genetics</topic><topic>PTEN Phosphohydrolase - metabolism</topic><topic>PTEN protein</topic><topic>Reporter gene</topic><topic>Staining</topic><topic>Tensins - genetics</topic><topic>Western blotting</topic><topic>X-Ray Microtomography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deng, Li</creatorcontrib><creatorcontrib>Huo, Peng‐cheng</creatorcontrib><creatorcontrib>Feng, Mei‐ting</creatorcontrib><creatorcontrib>Wang, Rui‐ling</creatorcontrib><creatorcontrib>Jing, Rui</creatorcontrib><creatorcontrib>Luo, Li‐jun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular oral microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deng, Li</au><au>Huo, Peng‐cheng</au><au>Feng, Mei‐ting</au><au>Wang, Rui‐ling</au><au>Jing, Rui</au><au>Luo, Li‐jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>miR‐27a‐5p alleviates periodontal inflammation by targeting phosphatase and tensin homolog deleted on chromosome ten</atitle><jtitle>Molecular oral microbiology</jtitle><addtitle>Mol Oral Microbiol</addtitle><date>2023-08</date><risdate>2023</risdate><volume>38</volume><issue>4</issue><spage>309</spage><epage>320</epage><pages>309-320</pages><issn>2041-1006</issn><eissn>2041-1014</eissn><abstract>Introduction
MicroRNAs (miRNAs), a type of non‐coding RNA, have been demonstrated to be essential posttranscriptional modulators in oral diseases and inflammatory responses. However, the specific role of miR‐27a‐5p in periodontitis requires further investigation. In this study, we used both cellular and animal models to determine how miR‐27a‐5p affects the pathogenesis of periodontitis and its associated biological functions.
Methods
Quantitative real‐time polymerase chain reaction and western blotting were used to analyze the expression of cytokines, phosphatase and tensin homolog deleted on chromosome ten (PTEN), and miR‐27a‐5p transcription. Investigation of alveolar bone resorption and inflammation of the periodontium in ligature‐induced periodontitis in mice was performed using micro‐computed tomography (micro‐CT), hematoxylin‐eosin (HE) staining, and tartrate‐resistant acid phosphatase (TRAP) staining. The binding of miR‐27a‐5p and PTEN was predicted using the TargetScan database and experimentally confirmed using dual luciferase reporter gene assays.
Results
The inflamed gingiva showed lower levels of miR‐27a‐5p. Macrophages from miR‐27a‐5p–/– mice produced much higher quantities of pro‐inflammatory cytokines owing to the stimulation of Porphyromonas gingivalis lipopolysaccharide, and miR‐27a‐5p–/– mice with ligature‐induced periodontitis also exhibited more severe alveolar bone resorption and damage to the periodontium. Target validation assays identified PTEN as a direct target of bona. Blocking PTEN expression partially reduced inflammation, both in vitro and in vivo.
Conclusions
miR‐27a‐5p alleviated the inflammatory response in periodontitis by targeting PTEN.
This work declares that miR‐27a‐5p alleviates periodontal inflammation by targeting phosphatase and tensin homolog deleted on chromosome ten in ligatured‐induced periodontitis mice and macrophages stimulated with Porphyromonas gingivalis lipopolysaccharide, which contributes to further exploration in the pathogenesis of periodontitis.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>37216657</pmid><doi>10.1111/omi.12416</doi><tpages>12</tpages></addata></record> |
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subjects | Acid phosphatase Acid phosphatase (tartrate-resistant) Acid resistance Alveolar bone alveolar bone loss Animal models Animals Bone resorption Bone Resorption - genetics Chromosomes Chromosomes - metabolism Computed tomography Cytokines Cytokines - genetics Gum disease Homology Inflammation Inflammatory response Lipopolysaccharides Macrophages Mice MicroRNAs MicroRNAs - genetics MicroRNAs - metabolism miRNA miR‐27a‐5p Modulators Pathogenesis Periodontitis Periodontitis - genetics Periodontium Phosphatase Polymerase chain reaction Post-transcription PTEN PTEN Phosphohydrolase - genetics PTEN Phosphohydrolase - metabolism PTEN protein Reporter gene Staining Tensins - genetics Western blotting X-Ray Microtomography |
title | miR‐27a‐5p alleviates periodontal inflammation by targeting phosphatase and tensin homolog deleted on chromosome ten |
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