Convivina is a specialised core gut symbiont of the invasive hornet Vespa velutina
We provide a culturomics analysis of the cultivable bacterial communities of the crop, midgut and hindgut compartments, as well as the ovaries, of the invasive insect Vespa velutina, along with a cultivation‐independent analysis of samples of the same nest through 16S rRNA amplicon sequencing. The V...
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| Veröffentlicht in: | Insect molecular biology 2023-10, Vol.32 (5), p.510-527 |
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| creator | Hettiarachchi, Amanda Cnockaert, Margo Joossens, Marie Laureys, David De Clippeleer, Jessika Vereecken, Nicolas J. Michez, Denis Smagghe, Guy Graaf, Dirk C. Vandamme, Peter |
| description | We provide a culturomics analysis of the cultivable bacterial communities of the crop, midgut and hindgut compartments, as well as the ovaries, of the invasive insect Vespa velutina, along with a cultivation‐independent analysis of samples of the same nest through 16S rRNA amplicon sequencing. The Vespa velutina bacterial symbiont community was dominated by the genera Convivina, Fructobacillus, Lactiplantibacillus, Lactococcus, Sphingomonas and Spiroplasma. Lactococcus lactis and Lactiplantibacillus plantarum represented generalist core lactic acid bacteria (LAB) symbionts, while Convivina species and Fructobacillus fructosus represented highly specialised core LAB symbionts with strongly reduced genome sizes. Sphingomonas and Spiroplasma were the only non‐LAB core symbionts but were not isolated. Convivina bacteria were particularly enriched in the hornet crop and included Convivina intestini, a species adapted towards amino acid metabolism, and Convivina praedatoris sp. nov. which was adapted towards carbohydrate metabolism.
Culturomics and 16S rRNA amplicon sequencing of crop, midgut, hindgut and ovary samples revealed Convivina, Fructobacillus, Lactiplantibacillus, Lactococcus, Sphingomonas and Spiroplasma as predominant bacterial symbionts of Vespa velutina in Belgium.
Lactococcus lactis and Lactiplantibacillus plantarum represented generalist core symbionts, while Convivina species and Fructobacillus fructosus represented highly specialised core symbionts with strongly reduced genome sizes.
Convivina bacteria were enriched in the crop and included Convivina intestini, a species adapted towards amino acid metabolism, and Convivina praedatoris sp. nov. a species was adapted towards carbohydrate metabolism. |
| doi_str_mv | 10.1111/imb.12847 |
| format | Article |
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Culturomics and 16S rRNA amplicon sequencing of crop, midgut, hindgut and ovary samples revealed Convivina, Fructobacillus, Lactiplantibacillus, Lactococcus, Sphingomonas and Spiroplasma as predominant bacterial symbionts of Vespa velutina in Belgium.
Lactococcus lactis and Lactiplantibacillus plantarum represented generalist core symbionts, while Convivina species and Fructobacillus fructosus represented highly specialised core symbionts with strongly reduced genome sizes.
Convivina bacteria were enriched in the crop and included Convivina intestini, a species adapted towards amino acid metabolism, and Convivina praedatoris sp. nov. a species was adapted towards carbohydrate metabolism.</description><identifier>ISSN: 0962-1075</identifier><identifier>EISSN: 1365-2583</identifier><identifier>DOI: 10.1111/imb.12847</identifier><identifier>PMID: 37204105</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Amino acids ; Bacteria ; Carbohydrate metabolism ; Carbohydrates ; Convivina ; culturomics ; Genomes ; gut symbiont ; Hindgut ; Insects ; invasive insect ; Invasive insects ; Invasive species ; Lactic acid ; Lactic acid bacteria ; Midgut ; New species ; Ovaries ; rRNA 16S ; Sphingomonas ; Spiroplasma ; Symbionts ; Vespa velutina</subject><ispartof>Insect molecular biology, 2023-10, Vol.32 (5), p.510-527</ispartof><rights>2023 The Authors. published by John Wiley & Sons Ltd on behalf of Royal Entomological Society.</rights><rights>2023 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society.</rights><rights>2023. This article is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3887-313a64785ed8e35dbc2a0cfaabf27970890f685a8e60a00b0e3e7c8879c82aee3</citedby><cites>FETCH-LOGICAL-c3887-313a64785ed8e35dbc2a0cfaabf27970890f685a8e60a00b0e3e7c8879c82aee3</cites><orcidid>0000-0001-8880-1838 ; 0000-0002-4577-6692 ; 0000-0001-8334-3313 ; 0000-0002-5581-7937</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fimb.12847$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fimb.12847$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37204105$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hettiarachchi, Amanda</creatorcontrib><creatorcontrib>Cnockaert, Margo</creatorcontrib><creatorcontrib>Joossens, Marie</creatorcontrib><creatorcontrib>Laureys, David</creatorcontrib><creatorcontrib>De Clippeleer, Jessika</creatorcontrib><creatorcontrib>Vereecken, Nicolas J.</creatorcontrib><creatorcontrib>Michez, Denis</creatorcontrib><creatorcontrib>Smagghe, Guy</creatorcontrib><creatorcontrib>Graaf, Dirk C.</creatorcontrib><creatorcontrib>Vandamme, Peter</creatorcontrib><title>Convivina is a specialised core gut symbiont of the invasive hornet Vespa velutina</title><title>Insect molecular biology</title><addtitle>Insect Mol Biol</addtitle><description>We provide a culturomics analysis of the cultivable bacterial communities of the crop, midgut and hindgut compartments, as well as the ovaries, of the invasive insect Vespa velutina, along with a cultivation‐independent analysis of samples of the same nest through 16S rRNA amplicon sequencing. The Vespa velutina bacterial symbiont community was dominated by the genera Convivina, Fructobacillus, Lactiplantibacillus, Lactococcus, Sphingomonas and Spiroplasma. Lactococcus lactis and Lactiplantibacillus plantarum represented generalist core lactic acid bacteria (LAB) symbionts, while Convivina species and Fructobacillus fructosus represented highly specialised core LAB symbionts with strongly reduced genome sizes. Sphingomonas and Spiroplasma were the only non‐LAB core symbionts but were not isolated. Convivina bacteria were particularly enriched in the hornet crop and included Convivina intestini, a species adapted towards amino acid metabolism, and Convivina praedatoris sp. nov. which was adapted towards carbohydrate metabolism.
Culturomics and 16S rRNA amplicon sequencing of crop, midgut, hindgut and ovary samples revealed Convivina, Fructobacillus, Lactiplantibacillus, Lactococcus, Sphingomonas and Spiroplasma as predominant bacterial symbionts of Vespa velutina in Belgium.
Lactococcus lactis and Lactiplantibacillus plantarum represented generalist core symbionts, while Convivina species and Fructobacillus fructosus represented highly specialised core symbionts with strongly reduced genome sizes.
Convivina bacteria were enriched in the crop and included Convivina intestini, a species adapted towards amino acid metabolism, and Convivina praedatoris sp. nov. a species was adapted towards carbohydrate metabolism.</description><subject>Amino acids</subject><subject>Bacteria</subject><subject>Carbohydrate metabolism</subject><subject>Carbohydrates</subject><subject>Convivina</subject><subject>culturomics</subject><subject>Genomes</subject><subject>gut symbiont</subject><subject>Hindgut</subject><subject>Insects</subject><subject>invasive insect</subject><subject>Invasive insects</subject><subject>Invasive species</subject><subject>Lactic acid</subject><subject>Lactic acid bacteria</subject><subject>Midgut</subject><subject>New species</subject><subject>Ovaries</subject><subject>rRNA 16S</subject><subject>Sphingomonas</subject><subject>Spiroplasma</subject><subject>Symbionts</subject><subject>Vespa velutina</subject><issn>0962-1075</issn><issn>1365-2583</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><recordid>eNp1kD1PwzAQQC0EoqUw8AeQJRYYQs9xYzsjVHxUAiEhYI0c9wKukrjESVD_PS4tDEjccsu7p9Mj5JjBBQsztlV-wWI1kTtkyLhIojhRfJcMIRVxxEAmA3Lg_QIAVCrSfTLgMoYJg2RInqau7m1va02tp5r6JRqrS-txTo1rkL51LfWrKreubqkraPuO1Na99rZH-u6aGlv6in6paY9l1wbRIdkrdOnxaLtH5OXm-nl6F90_3s6ml_eR4UrJiDOuxUSqBOcKeTLPTazBFFrnRSxTGV6FQqhEKxSgAXJAjtKEy9SoWCPyETnbeJeN--jQt1llvcGy1DW6zmexYkIKBiwN6OkfdOG6pg7fBUpAss7BA3W-oUzjvG-wyJaNrXSzyhhk69BZCJ19hw7sydbY5RXOf8mfsgEYb4BPW-Lqf1M2e7jaKL8ALZOGtw</recordid><startdate>202310</startdate><enddate>202310</enddate><creator>Hettiarachchi, Amanda</creator><creator>Cnockaert, Margo</creator><creator>Joossens, Marie</creator><creator>Laureys, David</creator><creator>De Clippeleer, Jessika</creator><creator>Vereecken, Nicolas J.</creator><creator>Michez, Denis</creator><creator>Smagghe, Guy</creator><creator>Graaf, Dirk C.</creator><creator>Vandamme, Peter</creator><general>John Wiley & Sons, Ltd</general><general>Blackwell Publishing Ltd</general><scope>24P</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8880-1838</orcidid><orcidid>https://orcid.org/0000-0002-4577-6692</orcidid><orcidid>https://orcid.org/0000-0001-8334-3313</orcidid><orcidid>https://orcid.org/0000-0002-5581-7937</orcidid></search><sort><creationdate>202310</creationdate><title>Convivina is a specialised core gut symbiont of the invasive hornet Vespa velutina</title><author>Hettiarachchi, Amanda ; Cnockaert, Margo ; Joossens, Marie ; Laureys, David ; De Clippeleer, Jessika ; Vereecken, Nicolas J. ; Michez, Denis ; Smagghe, Guy ; Graaf, Dirk C. ; Vandamme, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3887-313a64785ed8e35dbc2a0cfaabf27970890f685a8e60a00b0e3e7c8879c82aee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Amino acids</topic><topic>Bacteria</topic><topic>Carbohydrate metabolism</topic><topic>Carbohydrates</topic><topic>Convivina</topic><topic>culturomics</topic><topic>Genomes</topic><topic>gut symbiont</topic><topic>Hindgut</topic><topic>Insects</topic><topic>invasive insect</topic><topic>Invasive insects</topic><topic>Invasive species</topic><topic>Lactic acid</topic><topic>Lactic acid bacteria</topic><topic>Midgut</topic><topic>New species</topic><topic>Ovaries</topic><topic>rRNA 16S</topic><topic>Sphingomonas</topic><topic>Spiroplasma</topic><topic>Symbionts</topic><topic>Vespa velutina</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hettiarachchi, Amanda</creatorcontrib><creatorcontrib>Cnockaert, Margo</creatorcontrib><creatorcontrib>Joossens, Marie</creatorcontrib><creatorcontrib>Laureys, David</creatorcontrib><creatorcontrib>De Clippeleer, Jessika</creatorcontrib><creatorcontrib>Vereecken, Nicolas J.</creatorcontrib><creatorcontrib>Michez, Denis</creatorcontrib><creatorcontrib>Smagghe, Guy</creatorcontrib><creatorcontrib>Graaf, Dirk C.</creatorcontrib><creatorcontrib>Vandamme, Peter</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Insect molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hettiarachchi, Amanda</au><au>Cnockaert, Margo</au><au>Joossens, Marie</au><au>Laureys, David</au><au>De Clippeleer, Jessika</au><au>Vereecken, Nicolas J.</au><au>Michez, Denis</au><au>Smagghe, Guy</au><au>Graaf, Dirk C.</au><au>Vandamme, Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Convivina is a specialised core gut symbiont of the invasive hornet Vespa velutina</atitle><jtitle>Insect molecular biology</jtitle><addtitle>Insect Mol Biol</addtitle><date>2023-10</date><risdate>2023</risdate><volume>32</volume><issue>5</issue><spage>510</spage><epage>527</epage><pages>510-527</pages><issn>0962-1075</issn><eissn>1365-2583</eissn><abstract>We provide a culturomics analysis of the cultivable bacterial communities of the crop, midgut and hindgut compartments, as well as the ovaries, of the invasive insect Vespa velutina, along with a cultivation‐independent analysis of samples of the same nest through 16S rRNA amplicon sequencing. The Vespa velutina bacterial symbiont community was dominated by the genera Convivina, Fructobacillus, Lactiplantibacillus, Lactococcus, Sphingomonas and Spiroplasma. Lactococcus lactis and Lactiplantibacillus plantarum represented generalist core lactic acid bacteria (LAB) symbionts, while Convivina species and Fructobacillus fructosus represented highly specialised core LAB symbionts with strongly reduced genome sizes. Sphingomonas and Spiroplasma were the only non‐LAB core symbionts but were not isolated. Convivina bacteria were particularly enriched in the hornet crop and included Convivina intestini, a species adapted towards amino acid metabolism, and Convivina praedatoris sp. nov. which was adapted towards carbohydrate metabolism.
Culturomics and 16S rRNA amplicon sequencing of crop, midgut, hindgut and ovary samples revealed Convivina, Fructobacillus, Lactiplantibacillus, Lactococcus, Sphingomonas and Spiroplasma as predominant bacterial symbionts of Vespa velutina in Belgium.
Lactococcus lactis and Lactiplantibacillus plantarum represented generalist core symbionts, while Convivina species and Fructobacillus fructosus represented highly specialised core symbionts with strongly reduced genome sizes.
Convivina bacteria were enriched in the crop and included Convivina intestini, a species adapted towards amino acid metabolism, and Convivina praedatoris sp. nov. a species was adapted towards carbohydrate metabolism.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>37204105</pmid><doi>10.1111/imb.12847</doi><tpages>18</tpages><orcidid>https://orcid.org/0000-0001-8880-1838</orcidid><orcidid>https://orcid.org/0000-0002-4577-6692</orcidid><orcidid>https://orcid.org/0000-0001-8334-3313</orcidid><orcidid>https://orcid.org/0000-0002-5581-7937</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Amino acids Bacteria Carbohydrate metabolism Carbohydrates Convivina culturomics Genomes gut symbiont Hindgut Insects invasive insect Invasive insects Invasive species Lactic acid Lactic acid bacteria Midgut New species Ovaries rRNA 16S Sphingomonas Spiroplasma Symbionts Vespa velutina |
| title | Convivina is a specialised core gut symbiont of the invasive hornet Vespa velutina |
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