Chalcone Schiff bases disrupt cell membrane integrity of Saccharomyces cerevisiae and Candida albicans cells
Chalcones have a variety of cellular protective and regulatory functions that may have therapeutic potential in many diseases. In addition, they are considered to affect key metabolic processes in pathogens. Nevertheless, our current knowledge of the action of these compounds against fungal cell is...
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| Veröffentlicht in: | Archives of microbiology 2023-06, Vol.205 (6), p.246-246, Article 246 |
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| description | Chalcones have a variety of cellular protective and regulatory functions that may have therapeutic potential in many diseases. In addition, they are considered to affect key metabolic processes in pathogens. Nevertheless, our current knowledge of the action of these compounds against fungal cell is scarce. Therefore, in this study, various substituted chalcone Schiff bases were investigated to reveal their cellular targets within the yeasts
Saccharomyces cerevisiae
and
Candida albicans
. First, their antifungal activities were determined via minimum inhibitory concentration method. Surprisingly, parent chalcone Schiff bases showed little or no antifungal activity, while the nitro-substituted derivatives were found to be highly active against yeast cells. Next, we set out to determine the cellular target of active compounds and tested the involvement of the cell wall and cell membrane in this process. Our conductivity assay confirmed that the yeast cell membrane was compromised, and that ion leakage occurred upon treatment with nitro-substituted chalcone Schiff bases. Therefore, the cell membrane came to the fore as a possible target for the active chalcone derivatives. We also showed that exogenous ergosterol added to the growth medium reduced the inhibitory effect of chalcones. Our findings open up new possibilities for the design of future antimicrobial agents based on this appealing backbone structure. |
| doi_str_mv | 10.1007/s00203-023-03584-y |
| format | Article |
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Saccharomyces cerevisiae
and
Candida albicans
. First, their antifungal activities were determined via minimum inhibitory concentration method. Surprisingly, parent chalcone Schiff bases showed little or no antifungal activity, while the nitro-substituted derivatives were found to be highly active against yeast cells. Next, we set out to determine the cellular target of active compounds and tested the involvement of the cell wall and cell membrane in this process. Our conductivity assay confirmed that the yeast cell membrane was compromised, and that ion leakage occurred upon treatment with nitro-substituted chalcone Schiff bases. Therefore, the cell membrane came to the fore as a possible target for the active chalcone derivatives. We also showed that exogenous ergosterol added to the growth medium reduced the inhibitory effect of chalcones. Our findings open up new possibilities for the design of future antimicrobial agents based on this appealing backbone structure.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-023-03584-y</identifier><identifier>PMID: 37209304</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Antifungal activity ; Antifungal Agents - chemistry ; Antifungal Agents - pharmacology ; Antimicrobial agents ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Candida albicans ; Cell Biology ; Cell Membrane ; Cell membranes ; Cell walls ; Chalcone - pharmacology ; Chalcones - chemistry ; Chalcones - pharmacology ; Ecology ; Ergosterol ; Fungicides ; Imines ; Life Sciences ; Membranes ; Microbial Ecology ; Microbial Sensitivity Tests ; Microbiology ; Minimum inhibitory concentration ; Original Paper ; Saccharomyces cerevisiae ; Schiff Bases - chemistry ; Schiff Bases - pharmacology ; Substitutes ; Yeast ; Yeasts</subject><ispartof>Archives of microbiology, 2023-06, Vol.205 (6), p.246-246, Article 246</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-80122ae15c46990d7b2b32211ceb02c8c35b4a563e1e98f01e9f13a506165eb73</citedby><cites>FETCH-LOGICAL-c375t-80122ae15c46990d7b2b32211ceb02c8c35b4a563e1e98f01e9f13a506165eb73</cites><orcidid>0000-0002-8621-3474</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-023-03584-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-023-03584-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37209304$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ergüden, Bengü</creatorcontrib><creatorcontrib>Lüleci, Hatice Büşra</creatorcontrib><creatorcontrib>Ünver, Yasemin</creatorcontrib><title>Chalcone Schiff bases disrupt cell membrane integrity of Saccharomyces cerevisiae and Candida albicans cells</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><addtitle>Arch Microbiol</addtitle><description>Chalcones have a variety of cellular protective and regulatory functions that may have therapeutic potential in many diseases. In addition, they are considered to affect key metabolic processes in pathogens. Nevertheless, our current knowledge of the action of these compounds against fungal cell is scarce. Therefore, in this study, various substituted chalcone Schiff bases were investigated to reveal their cellular targets within the yeasts
Saccharomyces cerevisiae
and
Candida albicans
. First, their antifungal activities were determined via minimum inhibitory concentration method. Surprisingly, parent chalcone Schiff bases showed little or no antifungal activity, while the nitro-substituted derivatives were found to be highly active against yeast cells. Next, we set out to determine the cellular target of active compounds and tested the involvement of the cell wall and cell membrane in this process. Our conductivity assay confirmed that the yeast cell membrane was compromised, and that ion leakage occurred upon treatment with nitro-substituted chalcone Schiff bases. Therefore, the cell membrane came to the fore as a possible target for the active chalcone derivatives. We also showed that exogenous ergosterol added to the growth medium reduced the inhibitory effect of chalcones. Our findings open up new possibilities for the design of future antimicrobial agents based on this appealing backbone structure.</description><subject>Antifungal activity</subject><subject>Antifungal Agents - chemistry</subject><subject>Antifungal Agents - pharmacology</subject><subject>Antimicrobial agents</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Candida albicans</subject><subject>Cell Biology</subject><subject>Cell Membrane</subject><subject>Cell membranes</subject><subject>Cell walls</subject><subject>Chalcone - pharmacology</subject><subject>Chalcones - chemistry</subject><subject>Chalcones - pharmacology</subject><subject>Ecology</subject><subject>Ergosterol</subject><subject>Fungicides</subject><subject>Imines</subject><subject>Life Sciences</subject><subject>Membranes</subject><subject>Microbial Ecology</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Minimum inhibitory concentration</subject><subject>Original Paper</subject><subject>Saccharomyces cerevisiae</subject><subject>Schiff Bases - chemistry</subject><subject>Schiff Bases - pharmacology</subject><subject>Substitutes</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kUlLBDEUhIMoOi5_wIMEvHhpfUl6SR9lcAPBgwreQpJ-7UR6GZNuof-9mRkX8OAhL4d8VamkCDlmcM4AiosAwEEkwOMSmUyTaYvMWCp4AgV_2SYzEMATWQqxR_ZDeANgXEq5S_ZEwaEUkM5IM1_oxvYd0ke7cHVNjQ4YaOWCH5cDtdg0tMXWeB0R1w346t0w0b6mj9rahfZ9O9kosOjxwwWnkequovM4XKWpboyzugtro3BIdmrdBDz62g_I8_XV0_w2uX-4uZtf3idWFNmQyJiTa2SZTfOyhKow3AjOGbNogFtpRWZSneUCGZayhjhrJnQGOcszNIU4IGcb36Xv30cMg2pdWCWIj-jHoLhkeZGVaZlH9PQP-taPvovp1hQXnMHKkG8o6_sQPNZq6V2r_aQYqFUXatOFil2odRdqiqKTL-vRtFj9SL4_PwJiA4R41L2i_737H9tPsFeUug</recordid><startdate>20230601</startdate><enddate>20230601</enddate><creator>Ergüden, Bengü</creator><creator>Lüleci, Hatice Büşra</creator><creator>Ünver, Yasemin</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8621-3474</orcidid></search><sort><creationdate>20230601</creationdate><title>Chalcone Schiff bases disrupt cell membrane integrity of Saccharomyces cerevisiae and Candida albicans cells</title><author>Ergüden, Bengü ; 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In addition, they are considered to affect key metabolic processes in pathogens. Nevertheless, our current knowledge of the action of these compounds against fungal cell is scarce. Therefore, in this study, various substituted chalcone Schiff bases were investigated to reveal their cellular targets within the yeasts
Saccharomyces cerevisiae
and
Candida albicans
. First, their antifungal activities were determined via minimum inhibitory concentration method. Surprisingly, parent chalcone Schiff bases showed little or no antifungal activity, while the nitro-substituted derivatives were found to be highly active against yeast cells. Next, we set out to determine the cellular target of active compounds and tested the involvement of the cell wall and cell membrane in this process. Our conductivity assay confirmed that the yeast cell membrane was compromised, and that ion leakage occurred upon treatment with nitro-substituted chalcone Schiff bases. Therefore, the cell membrane came to the fore as a possible target for the active chalcone derivatives. We also showed that exogenous ergosterol added to the growth medium reduced the inhibitory effect of chalcones. Our findings open up new possibilities for the design of future antimicrobial agents based on this appealing backbone structure.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>37209304</pmid><doi>10.1007/s00203-023-03584-y</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-8621-3474</orcidid></addata></record> |
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| subjects | Antifungal activity Antifungal Agents - chemistry Antifungal Agents - pharmacology Antimicrobial agents Biochemistry Biomedical and Life Sciences Biotechnology Candida albicans Cell Biology Cell Membrane Cell membranes Cell walls Chalcone - pharmacology Chalcones - chemistry Chalcones - pharmacology Ecology Ergosterol Fungicides Imines Life Sciences Membranes Microbial Ecology Microbial Sensitivity Tests Microbiology Minimum inhibitory concentration Original Paper Saccharomyces cerevisiae Schiff Bases - chemistry Schiff Bases - pharmacology Substitutes Yeast Yeasts |
| title | Chalcone Schiff bases disrupt cell membrane integrity of Saccharomyces cerevisiae and Candida albicans cells |
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