Piezo1 mediates the degradation of cartilage extracellular matrix in malocclusion‐induced TMJOA
Objectives To evaluate the role of Piezo1 in the malocclusion‐induced osteoarthritic cartilage of the temporomandibular joint. Methods A temporomandibular joint osteoarthritis model was established using a unilateral anterior crossbite in vivo, and cartilage degeneration and Piezo1 expression were o...
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| Veröffentlicht in: | Oral diseases 2024-05, Vol.30 (4), p.2425-2438 |
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| creator | Feng, Xu Li, Siwen Wang, Shuangshuang Meng, Yuan Zheng, Shize Liu, Cangwei Chang, Bei Shi, Ce Sun, Hongchen |
| description | Objectives
To evaluate the role of Piezo1 in the malocclusion‐induced osteoarthritic cartilage of the temporomandibular joint.
Methods
A temporomandibular joint osteoarthritis model was established using a unilateral anterior crossbite in vivo, and cartilage degeneration and Piezo1 expression were observed by histological and immunohistochemical staining. ATDC5 cells were loaded with 24 dyn/cm2 fluid flow shear stress using the Flexcell device in vitro and expression and function of Piezo1 were evaluated. After identifying the function of Piezo1 in YAP translocation under FFSS conditions, the influence of Piezo1 and YAP on metabolism‐related enzymes under FFSS was detected through a real‐time polymerase chain reaction analysis and western blotting. A UAC‐TMJ injection model was established to observe the therapeutic effect of intra‐articular injection of a Piezo1 inhibitor on osteoarthritic cartilage matrix loss.
Results
Piezo1 was overexpressed in the osteoarthritic cartilage and cultured chondrocytes under shear stress. Piezo1 Silencing inhibited the nuclear translocation of YAP and subsequently downregulated the expression of MMP13 and ADAMTS5. Intra‐articular injection of the Piezo1 inhibitor, GsMTx4, could ameliorate proteoglycan degradation in malocclusion‐induced TMJOA and suppressed MMP13 and ADAMTS5 expression.
Conclusions
Our results revealed that the activation of Piezo1 promotes mechanical‐induced cartilage degradation through the YAP‐MMP13/ADAMTS5 signaling pathway. |
| doi_str_mv | 10.1111/odi.14615 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2813888970</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2813888970</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3535-466ddcb910b592b157be4eabec00731f41ef9269cf7f4c46a10048693361cb873</originalsourceid><addsrcrecordid>eNp1kM9OFTEUhxuCAUQWvABpwkYXAz3TP9MuCahgMNcFJu6aTucMlsydQjsTwZWP4DP6JPZywQWJZ3N-i-_8cvIRsg_sCMocxy4cgVAgN8gOKAYV07XcLJlLUcmaf9smr3O-YQwaw-stss0b0IIJuUPcl4A_I9AldsFNmOn0HWmH18l1bgpxpLGn3qUpDO4aKd5PyXkchnlwiS7dlMI9DWNJQ_R-mHO5-PPrdxi72WNHrz5_Wpy8Ia96N2Tce9q75OuH91en59Xl4uPF6cll5bnkshJKdZ1vDbBWmroF2bQo0LXoGWs49AKwN7Uyvm964YVywJjQynCuwLe64bvk7br3NsW7GfNklyGvfnUjxjnbWgPXWpuGFfTwBXoT5zSW7yxnqhbCGL2i3q0pn2LOCXt7m8LSpQcLzK682-LdPnov7MFT49wWlf_IZ9EFOF4DP8KAD_9vsouzi3XlX5jVjMM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3062449980</pqid></control><display><type>article</type><title>Piezo1 mediates the degradation of cartilage extracellular matrix in malocclusion‐induced TMJOA</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Feng, Xu ; Li, Siwen ; Wang, Shuangshuang ; Meng, Yuan ; Zheng, Shize ; Liu, Cangwei ; Chang, Bei ; Shi, Ce ; Sun, Hongchen</creator><creatorcontrib>Feng, Xu ; Li, Siwen ; Wang, Shuangshuang ; Meng, Yuan ; Zheng, Shize ; Liu, Cangwei ; Chang, Bei ; Shi, Ce ; Sun, Hongchen</creatorcontrib><description>Objectives
To evaluate the role of Piezo1 in the malocclusion‐induced osteoarthritic cartilage of the temporomandibular joint.
Methods
A temporomandibular joint osteoarthritis model was established using a unilateral anterior crossbite in vivo, and cartilage degeneration and Piezo1 expression were observed by histological and immunohistochemical staining. ATDC5 cells were loaded with 24 dyn/cm2 fluid flow shear stress using the Flexcell device in vitro and expression and function of Piezo1 were evaluated. After identifying the function of Piezo1 in YAP translocation under FFSS conditions, the influence of Piezo1 and YAP on metabolism‐related enzymes under FFSS was detected through a real‐time polymerase chain reaction analysis and western blotting. A UAC‐TMJ injection model was established to observe the therapeutic effect of intra‐articular injection of a Piezo1 inhibitor on osteoarthritic cartilage matrix loss.
Results
Piezo1 was overexpressed in the osteoarthritic cartilage and cultured chondrocytes under shear stress. Piezo1 Silencing inhibited the nuclear translocation of YAP and subsequently downregulated the expression of MMP13 and ADAMTS5. Intra‐articular injection of the Piezo1 inhibitor, GsMTx4, could ameliorate proteoglycan degradation in malocclusion‐induced TMJOA and suppressed MMP13 and ADAMTS5 expression.
Conclusions
Our results revealed that the activation of Piezo1 promotes mechanical‐induced cartilage degradation through the YAP‐MMP13/ADAMTS5 signaling pathway.</description><identifier>ISSN: 1354-523X</identifier><identifier>ISSN: 1601-0825</identifier><identifier>EISSN: 1601-0825</identifier><identifier>DOI: 10.1111/odi.14615</identifier><identifier>PMID: 37184045</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>ADAMTS5 Protein - genetics ; ADAMTS5 Protein - metabolism ; Animals ; Cartilage ; Cartilage diseases ; Cartilage, Articular - metabolism ; Cell Line ; Chondrocytes ; Chondrocytes - metabolism ; Collagenase 3 ; Degradation ; Dental occlusion ; Disease Models, Animal ; Extracellular matrix ; Extracellular Matrix - metabolism ; Fluid flow ; Histology ; Immunohistochemistry ; Injection ; Injections, Intra-Articular ; Ion Channels - metabolism ; Male ; malocclusion ; Malocclusion - metabolism ; Matrix Metalloproteinase 13 - metabolism ; mechanotransduction ; Mice ; Nuclear transport ; Osteoarthritis ; Osteoarthritis - metabolism ; Piezo1 ; Proteoglycans ; Shear stress ; Signal transduction ; Stress, Mechanical ; Temporomandibular joint ; Temporomandibular Joint Disorders - metabolism ; Western blotting ; YAP-Signaling Proteins - metabolism ; Yes-associated protein</subject><ispartof>Oral diseases, 2024-05, Vol.30 (4), p.2425-2438</ispartof><rights>2023 Wiley Periodicals LLC.</rights><rights>2024 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3535-466ddcb910b592b157be4eabec00731f41ef9269cf7f4c46a10048693361cb873</citedby><cites>FETCH-LOGICAL-c3535-466ddcb910b592b157be4eabec00731f41ef9269cf7f4c46a10048693361cb873</cites><orcidid>0000-0002-6866-5493</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fodi.14615$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fodi.14615$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37184045$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Feng, Xu</creatorcontrib><creatorcontrib>Li, Siwen</creatorcontrib><creatorcontrib>Wang, Shuangshuang</creatorcontrib><creatorcontrib>Meng, Yuan</creatorcontrib><creatorcontrib>Zheng, Shize</creatorcontrib><creatorcontrib>Liu, Cangwei</creatorcontrib><creatorcontrib>Chang, Bei</creatorcontrib><creatorcontrib>Shi, Ce</creatorcontrib><creatorcontrib>Sun, Hongchen</creatorcontrib><title>Piezo1 mediates the degradation of cartilage extracellular matrix in malocclusion‐induced TMJOA</title><title>Oral diseases</title><addtitle>Oral Dis</addtitle><description>Objectives
To evaluate the role of Piezo1 in the malocclusion‐induced osteoarthritic cartilage of the temporomandibular joint.
Methods
A temporomandibular joint osteoarthritis model was established using a unilateral anterior crossbite in vivo, and cartilage degeneration and Piezo1 expression were observed by histological and immunohistochemical staining. ATDC5 cells were loaded with 24 dyn/cm2 fluid flow shear stress using the Flexcell device in vitro and expression and function of Piezo1 were evaluated. After identifying the function of Piezo1 in YAP translocation under FFSS conditions, the influence of Piezo1 and YAP on metabolism‐related enzymes under FFSS was detected through a real‐time polymerase chain reaction analysis and western blotting. A UAC‐TMJ injection model was established to observe the therapeutic effect of intra‐articular injection of a Piezo1 inhibitor on osteoarthritic cartilage matrix loss.
Results
Piezo1 was overexpressed in the osteoarthritic cartilage and cultured chondrocytes under shear stress. Piezo1 Silencing inhibited the nuclear translocation of YAP and subsequently downregulated the expression of MMP13 and ADAMTS5. Intra‐articular injection of the Piezo1 inhibitor, GsMTx4, could ameliorate proteoglycan degradation in malocclusion‐induced TMJOA and suppressed MMP13 and ADAMTS5 expression.
Conclusions
Our results revealed that the activation of Piezo1 promotes mechanical‐induced cartilage degradation through the YAP‐MMP13/ADAMTS5 signaling pathway.</description><subject>ADAMTS5 Protein - genetics</subject><subject>ADAMTS5 Protein - metabolism</subject><subject>Animals</subject><subject>Cartilage</subject><subject>Cartilage diseases</subject><subject>Cartilage, Articular - metabolism</subject><subject>Cell Line</subject><subject>Chondrocytes</subject><subject>Chondrocytes - metabolism</subject><subject>Collagenase 3</subject><subject>Degradation</subject><subject>Dental occlusion</subject><subject>Disease Models, Animal</subject><subject>Extracellular matrix</subject><subject>Extracellular Matrix - metabolism</subject><subject>Fluid flow</subject><subject>Histology</subject><subject>Immunohistochemistry</subject><subject>Injection</subject><subject>Injections, Intra-Articular</subject><subject>Ion Channels - metabolism</subject><subject>Male</subject><subject>malocclusion</subject><subject>Malocclusion - metabolism</subject><subject>Matrix Metalloproteinase 13 - metabolism</subject><subject>mechanotransduction</subject><subject>Mice</subject><subject>Nuclear transport</subject><subject>Osteoarthritis</subject><subject>Osteoarthritis - metabolism</subject><subject>Piezo1</subject><subject>Proteoglycans</subject><subject>Shear stress</subject><subject>Signal transduction</subject><subject>Stress, Mechanical</subject><subject>Temporomandibular joint</subject><subject>Temporomandibular Joint Disorders - metabolism</subject><subject>Western blotting</subject><subject>YAP-Signaling Proteins - metabolism</subject><subject>Yes-associated protein</subject><issn>1354-523X</issn><issn>1601-0825</issn><issn>1601-0825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM9OFTEUhxuCAUQWvABpwkYXAz3TP9MuCahgMNcFJu6aTucMlsydQjsTwZWP4DP6JPZywQWJZ3N-i-_8cvIRsg_sCMocxy4cgVAgN8gOKAYV07XcLJlLUcmaf9smr3O-YQwaw-stss0b0IIJuUPcl4A_I9AldsFNmOn0HWmH18l1bgpxpLGn3qUpDO4aKd5PyXkchnlwiS7dlMI9DWNJQ_R-mHO5-PPrdxi72WNHrz5_Wpy8Ia96N2Tce9q75OuH91en59Xl4uPF6cll5bnkshJKdZ1vDbBWmroF2bQo0LXoGWs49AKwN7Uyvm964YVywJjQynCuwLe64bvk7br3NsW7GfNklyGvfnUjxjnbWgPXWpuGFfTwBXoT5zSW7yxnqhbCGL2i3q0pn2LOCXt7m8LSpQcLzK682-LdPnov7MFT49wWlf_IZ9EFOF4DP8KAD_9vsouzi3XlX5jVjMM</recordid><startdate>202405</startdate><enddate>202405</enddate><creator>Feng, Xu</creator><creator>Li, Siwen</creator><creator>Wang, Shuangshuang</creator><creator>Meng, Yuan</creator><creator>Zheng, Shize</creator><creator>Liu, Cangwei</creator><creator>Chang, Bei</creator><creator>Shi, Ce</creator><creator>Sun, Hongchen</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6866-5493</orcidid></search><sort><creationdate>202405</creationdate><title>Piezo1 mediates the degradation of cartilage extracellular matrix in malocclusion‐induced TMJOA</title><author>Feng, Xu ; Li, Siwen ; Wang, Shuangshuang ; Meng, Yuan ; Zheng, Shize ; Liu, Cangwei ; Chang, Bei ; Shi, Ce ; Sun, Hongchen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3535-466ddcb910b592b157be4eabec00731f41ef9269cf7f4c46a10048693361cb873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>ADAMTS5 Protein - genetics</topic><topic>ADAMTS5 Protein - metabolism</topic><topic>Animals</topic><topic>Cartilage</topic><topic>Cartilage diseases</topic><topic>Cartilage, Articular - metabolism</topic><topic>Cell Line</topic><topic>Chondrocytes</topic><topic>Chondrocytes - metabolism</topic><topic>Collagenase 3</topic><topic>Degradation</topic><topic>Dental occlusion</topic><topic>Disease Models, Animal</topic><topic>Extracellular matrix</topic><topic>Extracellular Matrix - metabolism</topic><topic>Fluid flow</topic><topic>Histology</topic><topic>Immunohistochemistry</topic><topic>Injection</topic><topic>Injections, Intra-Articular</topic><topic>Ion Channels - metabolism</topic><topic>Male</topic><topic>malocclusion</topic><topic>Malocclusion - metabolism</topic><topic>Matrix Metalloproteinase 13 - metabolism</topic><topic>mechanotransduction</topic><topic>Mice</topic><topic>Nuclear transport</topic><topic>Osteoarthritis</topic><topic>Osteoarthritis - metabolism</topic><topic>Piezo1</topic><topic>Proteoglycans</topic><topic>Shear stress</topic><topic>Signal transduction</topic><topic>Stress, Mechanical</topic><topic>Temporomandibular joint</topic><topic>Temporomandibular Joint Disorders - metabolism</topic><topic>Western blotting</topic><topic>YAP-Signaling Proteins - metabolism</topic><topic>Yes-associated protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Feng, Xu</creatorcontrib><creatorcontrib>Li, Siwen</creatorcontrib><creatorcontrib>Wang, Shuangshuang</creatorcontrib><creatorcontrib>Meng, Yuan</creatorcontrib><creatorcontrib>Zheng, Shize</creatorcontrib><creatorcontrib>Liu, Cangwei</creatorcontrib><creatorcontrib>Chang, Bei</creatorcontrib><creatorcontrib>Shi, Ce</creatorcontrib><creatorcontrib>Sun, Hongchen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Oral diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Feng, Xu</au><au>Li, Siwen</au><au>Wang, Shuangshuang</au><au>Meng, Yuan</au><au>Zheng, Shize</au><au>Liu, Cangwei</au><au>Chang, Bei</au><au>Shi, Ce</au><au>Sun, Hongchen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Piezo1 mediates the degradation of cartilage extracellular matrix in malocclusion‐induced TMJOA</atitle><jtitle>Oral diseases</jtitle><addtitle>Oral Dis</addtitle><date>2024-05</date><risdate>2024</risdate><volume>30</volume><issue>4</issue><spage>2425</spage><epage>2438</epage><pages>2425-2438</pages><issn>1354-523X</issn><issn>1601-0825</issn><eissn>1601-0825</eissn><abstract>Objectives
To evaluate the role of Piezo1 in the malocclusion‐induced osteoarthritic cartilage of the temporomandibular joint.
Methods
A temporomandibular joint osteoarthritis model was established using a unilateral anterior crossbite in vivo, and cartilage degeneration and Piezo1 expression were observed by histological and immunohistochemical staining. ATDC5 cells were loaded with 24 dyn/cm2 fluid flow shear stress using the Flexcell device in vitro and expression and function of Piezo1 were evaluated. After identifying the function of Piezo1 in YAP translocation under FFSS conditions, the influence of Piezo1 and YAP on metabolism‐related enzymes under FFSS was detected through a real‐time polymerase chain reaction analysis and western blotting. A UAC‐TMJ injection model was established to observe the therapeutic effect of intra‐articular injection of a Piezo1 inhibitor on osteoarthritic cartilage matrix loss.
Results
Piezo1 was overexpressed in the osteoarthritic cartilage and cultured chondrocytes under shear stress. Piezo1 Silencing inhibited the nuclear translocation of YAP and subsequently downregulated the expression of MMP13 and ADAMTS5. Intra‐articular injection of the Piezo1 inhibitor, GsMTx4, could ameliorate proteoglycan degradation in malocclusion‐induced TMJOA and suppressed MMP13 and ADAMTS5 expression.
Conclusions
Our results revealed that the activation of Piezo1 promotes mechanical‐induced cartilage degradation through the YAP‐MMP13/ADAMTS5 signaling pathway.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>37184045</pmid><doi>10.1111/odi.14615</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-6866-5493</orcidid></addata></record> |
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| subjects | ADAMTS5 Protein - genetics ADAMTS5 Protein - metabolism Animals Cartilage Cartilage diseases Cartilage, Articular - metabolism Cell Line Chondrocytes Chondrocytes - metabolism Collagenase 3 Degradation Dental occlusion Disease Models, Animal Extracellular matrix Extracellular Matrix - metabolism Fluid flow Histology Immunohistochemistry Injection Injections, Intra-Articular Ion Channels - metabolism Male malocclusion Malocclusion - metabolism Matrix Metalloproteinase 13 - metabolism mechanotransduction Mice Nuclear transport Osteoarthritis Osteoarthritis - metabolism Piezo1 Proteoglycans Shear stress Signal transduction Stress, Mechanical Temporomandibular joint Temporomandibular Joint Disorders - metabolism Western blotting YAP-Signaling Proteins - metabolism Yes-associated protein |
| title | Piezo1 mediates the degradation of cartilage extracellular matrix in malocclusion‐induced TMJOA |
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