Isolation, characterization, and application of lytic bacteriophages for controlling Enterobacter cloacae complex (ECC) in pasteurized milk and yogurt
Reducing bacterial pathogen contamination not only improves overall global public health but also diminishes food waste and loss. The use of lytic bacteriophages (phages) that infect and kill bacteria could be a beneficial tool for suppressing bacterial growth during dairy products storage time. Fou...
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| Veröffentlicht in: | Folia microbiologica 2023-12, Vol.68 (6), p.911-924 |
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| creator | Nasr-Eldin, Mohamed A. Gamal, Eman Hazza, Mahmoud Abo-Elmaaty, Sabah A. |
| description | Reducing bacterial pathogen contamination not only improves overall global public health but also diminishes food waste and loss. The use of lytic bacteriophages (phages) that infect and kill bacteria could be a beneficial tool for suppressing bacterial growth during dairy products storage time. Four
Enterobacter cloacae
(
E. cloacae
) complex isolates which were previously isolated from contaminated dairy products were used to identify lytic phages in wastewater. Phages specific to multi-drug resistant (MDR)
E. cloacae
complex 6AS1 were isolated from local sewage. Two novel phages vB_EclM-EP1 and vB_EclM-EP2 were identified as myoviral particles and have double-stranded DNA genome. Their host range and lytic capabilities were detected using spot test and efficiency of plating (EOP) against several bacterial isolates. The phages had a latent period of 30 min, and a large burst size of about 100 and 142 PFU/cell for vB_EclM-EP1 and vB_EclM-EP2, respectively. Both phages were viable at pH ranging 5–9 and stable at 70 °C for 60 min. The individual phages and their cocktail preparations (vB_EclM-EP1 and vB_EclM-EP2) reduced and inhibited the growth of
E. cloacae
complex 6AS1 during challenge test in milk and yogurt samples. These results indicate that the
E. cloacae
complex-specific phages (vB_EclM-EP1 and vB_EclM-EP2) have a potential application as microbicidal agents in packaged milk and milk derivatives during storage time. In addition, our environment is a rich sources of lytic phages which have potential use in eliminating multidrug-resistant isolates in food industry as well as in biocontrol. |
| doi_str_mv | 10.1007/s12223-023-01059-7 |
| format | Article |
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Enterobacter cloacae
(
E. cloacae
) complex isolates which were previously isolated from contaminated dairy products were used to identify lytic phages in wastewater. Phages specific to multi-drug resistant (MDR)
E. cloacae
complex 6AS1 were isolated from local sewage. Two novel phages vB_EclM-EP1 and vB_EclM-EP2 were identified as myoviral particles and have double-stranded DNA genome. Their host range and lytic capabilities were detected using spot test and efficiency of plating (EOP) against several bacterial isolates. The phages had a latent period of 30 min, and a large burst size of about 100 and 142 PFU/cell for vB_EclM-EP1 and vB_EclM-EP2, respectively. Both phages were viable at pH ranging 5–9 and stable at 70 °C for 60 min. The individual phages and their cocktail preparations (vB_EclM-EP1 and vB_EclM-EP2) reduced and inhibited the growth of
E. cloacae
complex 6AS1 during challenge test in milk and yogurt samples. These results indicate that the
E. cloacae
complex-specific phages (vB_EclM-EP1 and vB_EclM-EP2) have a potential application as microbicidal agents in packaged milk and milk derivatives during storage time. In addition, our environment is a rich sources of lytic phages which have potential use in eliminating multidrug-resistant isolates in food industry as well as in biocontrol.</description><identifier>ISSN: 0015-5632</identifier><identifier>ISSN: 1874-9356</identifier><identifier>EISSN: 1874-9356</identifier><identifier>DOI: 10.1007/s12223-023-01059-7</identifier><identifier>PMID: 37184760</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animals ; Applied Microbiology ; Bacteria ; Bacteriophages - genetics ; Biological control ; Biomedical and Life Sciences ; Burst size ; Dairy products ; Drug resistance ; Enterobacter cloacae ; Environmental Engineering/Biotechnology ; Food contamination ; Food industry ; Food waste ; Genomes ; Host range ; Immunology ; Latent period ; Life Sciences ; Microbicides ; Microbiology ; Milk ; Milk - microbiology ; Multidrug resistance ; Original Article ; Pasteurization ; Pasteurized milk ; Phages ; Public health ; Reagents ; Refuse Disposal ; Sewage ; Yogurt</subject><ispartof>Folia microbiologica, 2023-12, Vol.68 (6), p.911-924</ispartof><rights>The Author(s) 2023. corrected publication 2023</rights><rights>2023. The Author(s).</rights><rights>The Author(s) 2023. corrected publication 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-35ad7b4e558191af5fb1ae0674ccedb9bc5152b736a4ebc2ff57eff3f0cdde833</citedby><cites>FETCH-LOGICAL-c419t-35ad7b4e558191af5fb1ae0674ccedb9bc5152b736a4ebc2ff57eff3f0cdde833</cites><orcidid>0000-0002-8537-9392</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12223-023-01059-7$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12223-023-01059-7$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37184760$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nasr-Eldin, Mohamed A.</creatorcontrib><creatorcontrib>Gamal, Eman</creatorcontrib><creatorcontrib>Hazza, Mahmoud</creatorcontrib><creatorcontrib>Abo-Elmaaty, Sabah A.</creatorcontrib><title>Isolation, characterization, and application of lytic bacteriophages for controlling Enterobacter cloacae complex (ECC) in pasteurized milk and yogurt</title><title>Folia microbiologica</title><addtitle>Folia Microbiol</addtitle><addtitle>Folia Microbiol (Praha)</addtitle><description>Reducing bacterial pathogen contamination not only improves overall global public health but also diminishes food waste and loss. The use of lytic bacteriophages (phages) that infect and kill bacteria could be a beneficial tool for suppressing bacterial growth during dairy products storage time. Four
Enterobacter cloacae
(
E. cloacae
) complex isolates which were previously isolated from contaminated dairy products were used to identify lytic phages in wastewater. Phages specific to multi-drug resistant (MDR)
E. cloacae
complex 6AS1 were isolated from local sewage. Two novel phages vB_EclM-EP1 and vB_EclM-EP2 were identified as myoviral particles and have double-stranded DNA genome. Their host range and lytic capabilities were detected using spot test and efficiency of plating (EOP) against several bacterial isolates. The phages had a latent period of 30 min, and a large burst size of about 100 and 142 PFU/cell for vB_EclM-EP1 and vB_EclM-EP2, respectively. Both phages were viable at pH ranging 5–9 and stable at 70 °C for 60 min. The individual phages and their cocktail preparations (vB_EclM-EP1 and vB_EclM-EP2) reduced and inhibited the growth of
E. cloacae
complex 6AS1 during challenge test in milk and yogurt samples. These results indicate that the
E. cloacae
complex-specific phages (vB_EclM-EP1 and vB_EclM-EP2) have a potential application as microbicidal agents in packaged milk and milk derivatives during storage time. In addition, our environment is a rich sources of lytic phages which have potential use in eliminating multidrug-resistant isolates in food industry as well as in biocontrol.</description><subject>Animals</subject><subject>Applied Microbiology</subject><subject>Bacteria</subject><subject>Bacteriophages - genetics</subject><subject>Biological control</subject><subject>Biomedical and Life Sciences</subject><subject>Burst size</subject><subject>Dairy products</subject><subject>Drug resistance</subject><subject>Enterobacter cloacae</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Food contamination</subject><subject>Food industry</subject><subject>Food waste</subject><subject>Genomes</subject><subject>Host range</subject><subject>Immunology</subject><subject>Latent period</subject><subject>Life Sciences</subject><subject>Microbicides</subject><subject>Microbiology</subject><subject>Milk</subject><subject>Milk - microbiology</subject><subject>Multidrug resistance</subject><subject>Original Article</subject><subject>Pasteurization</subject><subject>Pasteurized milk</subject><subject>Phages</subject><subject>Public health</subject><subject>Reagents</subject><subject>Refuse Disposal</subject><subject>Sewage</subject><subject>Yogurt</subject><issn>0015-5632</issn><issn>1874-9356</issn><issn>1874-9356</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc-KFDEQxoMo7uzoC3iQgJcVbM3fTvdRhnFdWPCi5yadrsxmTSdt0g2OD-LzmtkeFTx4KAJVv_q-Ih9CLyh5SwlR7zJljPGKnIoS2VbqEdrQRomq5bJ-jDaEUFnJmrMLdJnzPSE1EZw9RRdc0UaommzQz5scvZ5dDG-wudNJmxmS-3Hu6DBgPU3emYcGjhb74-wM7lcuTnf6ABnbmLCJYU7RexcOeB_KNK4QNj5qo6EA4-ThO77a73avsQt40nmGpbjBgEfnvz7YHeNhSfMz9MRqn-H5-d2iLx_2n3cfq9tP1ze797eVEbSdKy71oHoBUja0pdpK21MNpFbCGBj6tjeSStYrXmsBvWHWSgXWckvMMEDD-RZdrbpTit8WyHM3umzAex0gLrljDeVNI9ryx1v06h_0Pi4plOsK1UqpGibqQrGVMinmnMB2U3KjTseOku6UWrem1pFTnVLrVFl6eZZe-hGGPyu_YyoAX4FcRuEA6a_3f2R_AcrppgU</recordid><startdate>20231201</startdate><enddate>20231201</enddate><creator>Nasr-Eldin, Mohamed A.</creator><creator>Gamal, Eman</creator><creator>Hazza, Mahmoud</creator><creator>Abo-Elmaaty, Sabah A.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8537-9392</orcidid></search><sort><creationdate>20231201</creationdate><title>Isolation, characterization, and application of lytic bacteriophages for controlling Enterobacter cloacae complex (ECC) in pasteurized milk and yogurt</title><author>Nasr-Eldin, Mohamed A. ; Gamal, Eman ; Hazza, Mahmoud ; Abo-Elmaaty, Sabah A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-35ad7b4e558191af5fb1ae0674ccedb9bc5152b736a4ebc2ff57eff3f0cdde833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Applied Microbiology</topic><topic>Bacteria</topic><topic>Bacteriophages - genetics</topic><topic>Biological control</topic><topic>Biomedical and Life Sciences</topic><topic>Burst size</topic><topic>Dairy products</topic><topic>Drug resistance</topic><topic>Enterobacter cloacae</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Food contamination</topic><topic>Food industry</topic><topic>Food waste</topic><topic>Genomes</topic><topic>Host range</topic><topic>Immunology</topic><topic>Latent period</topic><topic>Life Sciences</topic><topic>Microbicides</topic><topic>Microbiology</topic><topic>Milk</topic><topic>Milk - microbiology</topic><topic>Multidrug resistance</topic><topic>Original Article</topic><topic>Pasteurization</topic><topic>Pasteurized milk</topic><topic>Phages</topic><topic>Public health</topic><topic>Reagents</topic><topic>Refuse Disposal</topic><topic>Sewage</topic><topic>Yogurt</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nasr-Eldin, Mohamed A.</creatorcontrib><creatorcontrib>Gamal, Eman</creatorcontrib><creatorcontrib>Hazza, Mahmoud</creatorcontrib><creatorcontrib>Abo-Elmaaty, Sabah A.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Folia microbiologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nasr-Eldin, Mohamed A.</au><au>Gamal, Eman</au><au>Hazza, Mahmoud</au><au>Abo-Elmaaty, Sabah A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation, characterization, and application of lytic bacteriophages for controlling Enterobacter cloacae complex (ECC) in pasteurized milk and yogurt</atitle><jtitle>Folia microbiologica</jtitle><stitle>Folia Microbiol</stitle><addtitle>Folia Microbiol (Praha)</addtitle><date>2023-12-01</date><risdate>2023</risdate><volume>68</volume><issue>6</issue><spage>911</spage><epage>924</epage><pages>911-924</pages><issn>0015-5632</issn><issn>1874-9356</issn><eissn>1874-9356</eissn><abstract>Reducing bacterial pathogen contamination not only improves overall global public health but also diminishes food waste and loss. The use of lytic bacteriophages (phages) that infect and kill bacteria could be a beneficial tool for suppressing bacterial growth during dairy products storage time. Four
Enterobacter cloacae
(
E. cloacae
) complex isolates which were previously isolated from contaminated dairy products were used to identify lytic phages in wastewater. Phages specific to multi-drug resistant (MDR)
E. cloacae
complex 6AS1 were isolated from local sewage. Two novel phages vB_EclM-EP1 and vB_EclM-EP2 were identified as myoviral particles and have double-stranded DNA genome. Their host range and lytic capabilities were detected using spot test and efficiency of plating (EOP) against several bacterial isolates. The phages had a latent period of 30 min, and a large burst size of about 100 and 142 PFU/cell for vB_EclM-EP1 and vB_EclM-EP2, respectively. Both phages were viable at pH ranging 5–9 and stable at 70 °C for 60 min. The individual phages and their cocktail preparations (vB_EclM-EP1 and vB_EclM-EP2) reduced and inhibited the growth of
E. cloacae
complex 6AS1 during challenge test in milk and yogurt samples. These results indicate that the
E. cloacae
complex-specific phages (vB_EclM-EP1 and vB_EclM-EP2) have a potential application as microbicidal agents in packaged milk and milk derivatives during storage time. In addition, our environment is a rich sources of lytic phages which have potential use in eliminating multidrug-resistant isolates in food industry as well as in biocontrol.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>37184760</pmid><doi>10.1007/s12223-023-01059-7</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-8537-9392</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Applied Microbiology Bacteria Bacteriophages - genetics Biological control Biomedical and Life Sciences Burst size Dairy products Drug resistance Enterobacter cloacae Environmental Engineering/Biotechnology Food contamination Food industry Food waste Genomes Host range Immunology Latent period Life Sciences Microbicides Microbiology Milk Milk - microbiology Multidrug resistance Original Article Pasteurization Pasteurized milk Phages Public health Reagents Refuse Disposal Sewage Yogurt |
| title | Isolation, characterization, and application of lytic bacteriophages for controlling Enterobacter cloacae complex (ECC) in pasteurized milk and yogurt |
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