Development of a gene-targeting system using CRISPR/Cas9 and utilization of pyrG as a novel selectable marker in Lentinula edodes

Abstract First, we attempted to recombine the Shiitake (Lentinula edodes) pyrG (ura3) gene homologously by introducing a donor vector containing a carboxin resistance gene (lecbxR) flanked by homologous sequences of pyrG into protoplasts of the fungus. However, all the carboxin-resistant transforman...

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Veröffentlicht in:	FEMS microbiology letters 2023-01, Vol.370
Hauptverfasser:	Kamiya, Ayane, Ueshima, Hiroki, Nishida, Shota, Honda, Yoichi, Kamitsuji, Hisatoshi, Sato, Toshitsugu, Miyamoto, Haruto, Sumita, Takuya, Izumitsu, Kosuke, Irie, Toshikazu
Format:	Artikel
Sprache:	eng
Schlagworte:	Carboxin Cassettes CRISPR CRISPR-Cas Systems Gene Editing - methods Gene Targeting Genome editing Genomes Homologous recombination Homology Lentinula edodes Microbiology Nucleotide sequence Protoplasts Shiitake Mushrooms - genetics Vectors (Biology)
Online-Zugang:	Volltext
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