Orientation of capture antibodies on gold nanoparticles to improve the sensitivity of ELISA-based medical devices
The sensitivity of ELISA-based devices strongly depends on the right orientation of antibodies on the sensor surface. The aim of this work was to increase the analytical performance of a commercial ELISA-based medical device (VIDAS®), thanks to the specific orientation of antibodies on gold nanostru...
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| Veröffentlicht in: | Talanta (Oxford) 2023-08, Vol.260, p.124650-124650, Article 124650 |
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| creator | Susini, Vanessa Ferraro, Giovanni Fierabracci, Vanna Ursino, Silvia Sanguinetti, Chiara Caponi, Laura Romiti, Nadia Rossi, Veronica Lucia Sanesi, Antonio Paolicchi, Aldo Franzini, Maria Fratini, Emiliano |
| description | The sensitivity of ELISA-based devices strongly depends on the right orientation of antibodies on the sensor surface. The aim of this work was to increase the analytical performance of a commercial ELISA-based medical device (VIDAS®), thanks to the specific orientation of antibodies on gold nanostructured disposables. For this purpose, fPSA VIDAS® assay was used as model and the disposable providing the antigen binding surface (SPR®) was functionalized with gold nanostructures coated with monovalent half-fragment antibodies (reduced IgG, rIgG). The functionalization of polystyrene SPRs® with gold nanostructures was achieved through a one-step incubation of gold dispersions in a mixture of non-toxic solvents. Five different concentrations of gold nanoparticles (NPs) were tested with a maximum fluorescence enhancement for NPs density around 3–8 *103 NPs/μm2 (752 ± 11 RFV vs 316 ± 5 RFV of bare SPRs®). The comparison of the dose-response curve obtained with commercial and gold coated-SPRs® revealed a significant improvement (p |
| doi_str_mv | 10.1016/j.talanta.2023.124650 |
| format | Article |
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[Display omitted]
•Simple and one-step deposition of gold-NPs on polystyrene using non-toxic solvents.•Half fragment antibodies are directly coated on the gold-NPs layered on polystyrene.•The improved ELISA-based medical device showed enhanced analytical sensitivity.•The method described results quick, simple, and potentially scalable.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2023.124650</identifier><identifier>PMID: 37167679</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Analytical sensitivity ; Antibodies - chemistry ; Biosensing Techniques ; Controlled plastic decoration ; Enzyme-Linked Immunosorbent Assay ; Gold - chemistry ; Gold nanoparticles ; Medical device ; Metal Nanoparticles - chemistry ; Oriented capture antibodies</subject><ispartof>Talanta (Oxford), 2023-08, Vol.260, p.124650-124650, Article 124650</ispartof><rights>2023 The Authors</rights><rights>Copyright © 2023. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c360t-f296dbd344fb54b93c6bc9ab6155de40a5b43426070f53f27b6918a9c9d7c963</cites><orcidid>0000-0002-9760-8180 ; 0000-0003-0102-5915 ; 0000-0002-8376-2486 ; 0000-0001-9496-9370</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0039914023004010$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37167679$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Susini, Vanessa</creatorcontrib><creatorcontrib>Ferraro, Giovanni</creatorcontrib><creatorcontrib>Fierabracci, Vanna</creatorcontrib><creatorcontrib>Ursino, Silvia</creatorcontrib><creatorcontrib>Sanguinetti, Chiara</creatorcontrib><creatorcontrib>Caponi, Laura</creatorcontrib><creatorcontrib>Romiti, Nadia</creatorcontrib><creatorcontrib>Rossi, Veronica Lucia</creatorcontrib><creatorcontrib>Sanesi, Antonio</creatorcontrib><creatorcontrib>Paolicchi, Aldo</creatorcontrib><creatorcontrib>Franzini, Maria</creatorcontrib><creatorcontrib>Fratini, Emiliano</creatorcontrib><title>Orientation of capture antibodies on gold nanoparticles to improve the sensitivity of ELISA-based medical devices</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>The sensitivity of ELISA-based devices strongly depends on the right orientation of antibodies on the sensor surface. The aim of this work was to increase the analytical performance of a commercial ELISA-based medical device (VIDAS®), thanks to the specific orientation of antibodies on gold nanostructured disposables. For this purpose, fPSA VIDAS® assay was used as model and the disposable providing the antigen binding surface (SPR®) was functionalized with gold nanostructures coated with monovalent half-fragment antibodies (reduced IgG, rIgG). The functionalization of polystyrene SPRs® with gold nanostructures was achieved through a one-step incubation of gold dispersions in a mixture of non-toxic solvents. Five different concentrations of gold nanoparticles (NPs) were tested with a maximum fluorescence enhancement for NPs density around 3–8 *103 NPs/μm2 (752 ± 11 RFV vs 316 ± 5 RFV of bare SPRs®). The comparison of the dose-response curve obtained with commercial and gold coated-SPRs® revealed a significant improvement (p < 0.0001) of the analytical sensitivity of the VIDAS® system using nanostructured disposables. This improved version of SPRs® allows to distinguish small variations of fPSA concentrations opening the way to the application of this biomarker to other kinds of cancer as recently described in the literature.
[Display omitted]
•Simple and one-step deposition of gold-NPs on polystyrene using non-toxic solvents.•Half fragment antibodies are directly coated on the gold-NPs layered on polystyrene.•The improved ELISA-based medical device showed enhanced analytical sensitivity.•The method described results quick, simple, and potentially scalable.</description><subject>Analytical sensitivity</subject><subject>Antibodies - chemistry</subject><subject>Biosensing Techniques</subject><subject>Controlled plastic decoration</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Gold - chemistry</subject><subject>Gold nanoparticles</subject><subject>Medical device</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Oriented capture antibodies</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1PAyEQhonRaK3-BA1HL1thWdjlZIzxK2nSg94JH7NKs10q0Cb-e2lavXqahHnmHeZB6IqSGSVU3C5nWQ96zHpWk5rNaN0ITo7QhHYtqxhv2TGaEMJkJWlDztB5SktCCknYKTpjLRWtaOUEfS2ih5KSfRhx6LHV67yJgEuyN8F5SLg0PsLg8KjHsNYxezuU1xywX61j2ALOn4ATjMlnv_X5exfzOH99u6-MTuDwCpy3esAOtt5CukAnvR4SXB7qFL0_Pb4_vFTzxfPrw_28skyQXPW1FM441jS94Y2RzApjpTaCcu6gIZqbhjW1IC3pOevr1ghJOy2tdK2Vgk3RzT62_PFrAymrlU8WhuIMwiapuqOM8052XUH5HrUxpBShV-voVzp-K0rUTrZaqoNstZOt9rLL3PVhxcaUI_-mfu0W4G4PQLlz6yGqZIttW4REsFm54P9Z8QMNJpRE</recordid><startdate>20230801</startdate><enddate>20230801</enddate><creator>Susini, Vanessa</creator><creator>Ferraro, Giovanni</creator><creator>Fierabracci, Vanna</creator><creator>Ursino, Silvia</creator><creator>Sanguinetti, Chiara</creator><creator>Caponi, Laura</creator><creator>Romiti, Nadia</creator><creator>Rossi, Veronica Lucia</creator><creator>Sanesi, Antonio</creator><creator>Paolicchi, Aldo</creator><creator>Franzini, Maria</creator><creator>Fratini, Emiliano</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9760-8180</orcidid><orcidid>https://orcid.org/0000-0003-0102-5915</orcidid><orcidid>https://orcid.org/0000-0002-8376-2486</orcidid><orcidid>https://orcid.org/0000-0001-9496-9370</orcidid></search><sort><creationdate>20230801</creationdate><title>Orientation of capture antibodies on gold nanoparticles to improve the sensitivity of ELISA-based medical devices</title><author>Susini, Vanessa ; Ferraro, Giovanni ; Fierabracci, Vanna ; Ursino, Silvia ; Sanguinetti, Chiara ; Caponi, Laura ; Romiti, Nadia ; Rossi, Veronica Lucia ; Sanesi, Antonio ; Paolicchi, Aldo ; Franzini, Maria ; Fratini, Emiliano</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-f296dbd344fb54b93c6bc9ab6155de40a5b43426070f53f27b6918a9c9d7c963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Analytical sensitivity</topic><topic>Antibodies - chemistry</topic><topic>Biosensing Techniques</topic><topic>Controlled plastic decoration</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Gold - chemistry</topic><topic>Gold nanoparticles</topic><topic>Medical device</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Oriented capture antibodies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Susini, Vanessa</creatorcontrib><creatorcontrib>Ferraro, Giovanni</creatorcontrib><creatorcontrib>Fierabracci, Vanna</creatorcontrib><creatorcontrib>Ursino, Silvia</creatorcontrib><creatorcontrib>Sanguinetti, Chiara</creatorcontrib><creatorcontrib>Caponi, Laura</creatorcontrib><creatorcontrib>Romiti, Nadia</creatorcontrib><creatorcontrib>Rossi, Veronica Lucia</creatorcontrib><creatorcontrib>Sanesi, Antonio</creatorcontrib><creatorcontrib>Paolicchi, Aldo</creatorcontrib><creatorcontrib>Franzini, Maria</creatorcontrib><creatorcontrib>Fratini, Emiliano</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Susini, Vanessa</au><au>Ferraro, Giovanni</au><au>Fierabracci, Vanna</au><au>Ursino, Silvia</au><au>Sanguinetti, Chiara</au><au>Caponi, Laura</au><au>Romiti, Nadia</au><au>Rossi, Veronica Lucia</au><au>Sanesi, Antonio</au><au>Paolicchi, Aldo</au><au>Franzini, Maria</au><au>Fratini, Emiliano</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Orientation of capture antibodies on gold nanoparticles to improve the sensitivity of ELISA-based medical devices</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2023-08-01</date><risdate>2023</risdate><volume>260</volume><spage>124650</spage><epage>124650</epage><pages>124650-124650</pages><artnum>124650</artnum><issn>0039-9140</issn><eissn>1873-3573</eissn><abstract>The sensitivity of ELISA-based devices strongly depends on the right orientation of antibodies on the sensor surface. The aim of this work was to increase the analytical performance of a commercial ELISA-based medical device (VIDAS®), thanks to the specific orientation of antibodies on gold nanostructured disposables. For this purpose, fPSA VIDAS® assay was used as model and the disposable providing the antigen binding surface (SPR®) was functionalized with gold nanostructures coated with monovalent half-fragment antibodies (reduced IgG, rIgG). The functionalization of polystyrene SPRs® with gold nanostructures was achieved through a one-step incubation of gold dispersions in a mixture of non-toxic solvents. Five different concentrations of gold nanoparticles (NPs) were tested with a maximum fluorescence enhancement for NPs density around 3–8 *103 NPs/μm2 (752 ± 11 RFV vs 316 ± 5 RFV of bare SPRs®). The comparison of the dose-response curve obtained with commercial and gold coated-SPRs® revealed a significant improvement (p < 0.0001) of the analytical sensitivity of the VIDAS® system using nanostructured disposables. This improved version of SPRs® allows to distinguish small variations of fPSA concentrations opening the way to the application of this biomarker to other kinds of cancer as recently described in the literature.
[Display omitted]
•Simple and one-step deposition of gold-NPs on polystyrene using non-toxic solvents.•Half fragment antibodies are directly coated on the gold-NPs layered on polystyrene.•The improved ELISA-based medical device showed enhanced analytical sensitivity.•The method described results quick, simple, and potentially scalable.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>37167679</pmid><doi>10.1016/j.talanta.2023.124650</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-9760-8180</orcidid><orcidid>https://orcid.org/0000-0003-0102-5915</orcidid><orcidid>https://orcid.org/0000-0002-8376-2486</orcidid><orcidid>https://orcid.org/0000-0001-9496-9370</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Analytical sensitivity Antibodies - chemistry Biosensing Techniques Controlled plastic decoration Enzyme-Linked Immunosorbent Assay Gold - chemistry Gold nanoparticles Medical device Metal Nanoparticles - chemistry Oriented capture antibodies |
| title | Orientation of capture antibodies on gold nanoparticles to improve the sensitivity of ELISA-based medical devices |
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