Mulberry leaf and konjac compound powder improves the metabolic capacity of old mice on a high‐protein diet by regulating the structure of the intestinal microbiota
BACKGROUND The health of elderly individuals is closely linked to their protein intake and the abundance of intestinal microbiota. To investigate the impact of a compound powder made from mulberry leaf and konjac (hereinafter referred to as ‘compound powder’) on regulating the structure of intestina...
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| Veröffentlicht in: | Journal of the science of food and agriculture 2023-09, Vol.103 (12), p.5949-5957 |
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| container_title | Journal of the science of food and agriculture |
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| creator | Deng, Liling Zhong, Geng Peng, Yongbo Zhu, Zhaojing |
| description | BACKGROUND
The health of elderly individuals is closely linked to their protein intake and the abundance of intestinal microbiota. To investigate the impact of a compound powder made from mulberry leaf and konjac (hereinafter referred to as ‘compound powder’) on regulating the structure of intestinal microbiota in 15‐month‐old BALB/c mice that were fed a high‐beef‐protein diet, 16S rRNA high‐throughput sequencing, reverse transcription quantitative polymerase reaction, western blot, and other biochemical methods were used to analyze the differences in intestinal microbiota, protein metabolism‐related genes, short‐chain fatty acid (SCFA) content, and serum cytokines.
RESULTS
The results showed that the compound powder increased the content of SCFAs, reduced the inflammatory reaction of the body, adjusted the abundance of intestinal microbiota (Firmicutes and Bacteroidetes), and increased the ratio of Firmicutes to Bacteroidetes (F/B). Moreover, the compound powder could increase the abundance of Lactobacillus and some non‐dominant bacteria that were related to amino acid metabolism and beneficial to human health, such as Eubacterium coprostanoligenes. These beneficial bacteria competitively reduced the abundance of harmful bacteria to protect the intestinal barrier and promote intestinal health, and upregulated the activities of aminopeptidase, proton‐coupled oligopeptide transporter 1, and glutamate dehydrogenase at the transcription and translation levels.
CONCLUSION
The compound powder could balance the abundance of intestinal microbiota, which may improve the metabolic capacity of old mice on a high‐protein diet, and ultimately promoting the well‐being of elderly individuals. © 2023 Society of Chemical Industry. |
| doi_str_mv | 10.1002/jsfa.12664 |
| format | Article |
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The health of elderly individuals is closely linked to their protein intake and the abundance of intestinal microbiota. To investigate the impact of a compound powder made from mulberry leaf and konjac (hereinafter referred to as ‘compound powder’) on regulating the structure of intestinal microbiota in 15‐month‐old BALB/c mice that were fed a high‐beef‐protein diet, 16S rRNA high‐throughput sequencing, reverse transcription quantitative polymerase reaction, western blot, and other biochemical methods were used to analyze the differences in intestinal microbiota, protein metabolism‐related genes, short‐chain fatty acid (SCFA) content, and serum cytokines.
RESULTS
The results showed that the compound powder increased the content of SCFAs, reduced the inflammatory reaction of the body, adjusted the abundance of intestinal microbiota (Firmicutes and Bacteroidetes), and increased the ratio of Firmicutes to Bacteroidetes (F/B). Moreover, the compound powder could increase the abundance of Lactobacillus and some non‐dominant bacteria that were related to amino acid metabolism and beneficial to human health, such as Eubacterium coprostanoligenes. These beneficial bacteria competitively reduced the abundance of harmful bacteria to protect the intestinal barrier and promote intestinal health, and upregulated the activities of aminopeptidase, proton‐coupled oligopeptide transporter 1, and glutamate dehydrogenase at the transcription and translation levels.
CONCLUSION
The compound powder could balance the abundance of intestinal microbiota, which may improve the metabolic capacity of old mice on a high‐protein diet, and ultimately promoting the well‐being of elderly individuals. © 2023 Society of Chemical Industry.</description><identifier>ISSN: 0022-5142</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/jsfa.12664</identifier><identifier>PMID: 37127926</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Abundance ; Amino acids ; Aminopeptidase ; Bacteria ; Bacteroidetes ; Diet ; digestion and metabolism ; Fatty acids ; Firmicutes ; Glutamate dehydrogenase ; High protein diet ; high‐beef‐protein diet ; Inflammation ; intestinal microbiota ; Intestinal microflora ; Intestine ; Leaves ; Metabolism ; Microbiota ; Microorganisms ; mulberry leaf and konjac compound powder ; old mice ; Older people ; Powder ; Protein metabolism ; Protein turnover ; Proteins ; Reverse transcription ; rRNA 16S</subject><ispartof>Journal of the science of food and agriculture, 2023-09, Vol.103 (12), p.5949-5957</ispartof><rights>2023 Society of Chemical Industry.</rights><rights>Copyright © 2023 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3574-229265e49bdf00972f166916309b83bcc52b7ae2bb685cd2a24f7689b023cc3</citedby><cites>FETCH-LOGICAL-c3574-229265e49bdf00972f166916309b83bcc52b7ae2bb685cd2a24f7689b023cc3</cites><orcidid>0000-0001-6202-6108</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjsfa.12664$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjsfa.12664$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37127926$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deng, Liling</creatorcontrib><creatorcontrib>Zhong, Geng</creatorcontrib><creatorcontrib>Peng, Yongbo</creatorcontrib><creatorcontrib>Zhu, Zhaojing</creatorcontrib><title>Mulberry leaf and konjac compound powder improves the metabolic capacity of old mice on a high‐protein diet by regulating the structure of the intestinal microbiota</title><title>Journal of the science of food and agriculture</title><addtitle>J Sci Food Agric</addtitle><description>BACKGROUND
The health of elderly individuals is closely linked to their protein intake and the abundance of intestinal microbiota. To investigate the impact of a compound powder made from mulberry leaf and konjac (hereinafter referred to as ‘compound powder’) on regulating the structure of intestinal microbiota in 15‐month‐old BALB/c mice that were fed a high‐beef‐protein diet, 16S rRNA high‐throughput sequencing, reverse transcription quantitative polymerase reaction, western blot, and other biochemical methods were used to analyze the differences in intestinal microbiota, protein metabolism‐related genes, short‐chain fatty acid (SCFA) content, and serum cytokines.
RESULTS
The results showed that the compound powder increased the content of SCFAs, reduced the inflammatory reaction of the body, adjusted the abundance of intestinal microbiota (Firmicutes and Bacteroidetes), and increased the ratio of Firmicutes to Bacteroidetes (F/B). Moreover, the compound powder could increase the abundance of Lactobacillus and some non‐dominant bacteria that were related to amino acid metabolism and beneficial to human health, such as Eubacterium coprostanoligenes. These beneficial bacteria competitively reduced the abundance of harmful bacteria to protect the intestinal barrier and promote intestinal health, and upregulated the activities of aminopeptidase, proton‐coupled oligopeptide transporter 1, and glutamate dehydrogenase at the transcription and translation levels.
CONCLUSION
The compound powder could balance the abundance of intestinal microbiota, which may improve the metabolic capacity of old mice on a high‐protein diet, and ultimately promoting the well‐being of elderly individuals. © 2023 Society of Chemical Industry.</description><subject>Abundance</subject><subject>Amino acids</subject><subject>Aminopeptidase</subject><subject>Bacteria</subject><subject>Bacteroidetes</subject><subject>Diet</subject><subject>digestion and metabolism</subject><subject>Fatty acids</subject><subject>Firmicutes</subject><subject>Glutamate dehydrogenase</subject><subject>High protein diet</subject><subject>high‐beef‐protein diet</subject><subject>Inflammation</subject><subject>intestinal microbiota</subject><subject>Intestinal microflora</subject><subject>Intestine</subject><subject>Leaves</subject><subject>Metabolism</subject><subject>Microbiota</subject><subject>Microorganisms</subject><subject>mulberry leaf and konjac compound powder</subject><subject>old mice</subject><subject>Older people</subject><subject>Powder</subject><subject>Protein metabolism</subject><subject>Protein turnover</subject><subject>Proteins</subject><subject>Reverse transcription</subject><subject>rRNA 16S</subject><issn>0022-5142</issn><issn>1097-0010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNp9kUFu1DAUhi1ERYfChgMgS2wQUortJHa8rCoKVK1YlH1kOy8zHpw42E6r7DgCp-BgnASn07Jgwcqy3-dP79eP0CtKTikh7P0-9uqUMs6rJ2hDiRQFIZQ8RZs8ZEVNK3aMnse4J4RIyfkzdFwKyoRkfIN-Xc9OQwgLdqB6rMYOf_PjXhls_DD5Od8nf9dBwHaYgr-FiNMO8ABJae9sxtSkjE0L9j32rsODNYD9iBXe2e3u94-f-VcCO-LOQsJ6wQG2s1PJjtt7U0xhNmkOsArWBzsmiHms3OoKXluf1At01CsX4eXDeYJuLj58Pf9UXH35-Pn87KowZS2qgrEcqoZK6q7PYQXrKeeS8pJI3ZTamJppoYBpzZvadEyxqhe8kZqw0pjyBL09WPPO3-e8RTvYaMA5NYKfY8sa0tSNqATN6Jt_0L2fQ156paqKEkqlyNS7A5VzxBigb6dgBxWWlpJ27a5du2vvu8vw6wflrAfo_qKPZWWAHoA762D5j6q9vLk4O0j_AHSTp4A</recordid><startdate>202309</startdate><enddate>202309</enddate><creator>Deng, Liling</creator><creator>Zhong, Geng</creator><creator>Peng, Yongbo</creator><creator>Zhu, Zhaojing</creator><general>John Wiley & Sons, Ltd</general><general>John Wiley and Sons, Limited</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QL</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7ST</scope><scope>7T5</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6202-6108</orcidid></search><sort><creationdate>202309</creationdate><title>Mulberry leaf and konjac compound powder improves the metabolic capacity of old mice on a high‐protein diet by regulating the structure of the intestinal microbiota</title><author>Deng, Liling ; Zhong, Geng ; Peng, Yongbo ; Zhu, Zhaojing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3574-229265e49bdf00972f166916309b83bcc52b7ae2bb685cd2a24f7689b023cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Abundance</topic><topic>Amino acids</topic><topic>Aminopeptidase</topic><topic>Bacteria</topic><topic>Bacteroidetes</topic><topic>Diet</topic><topic>digestion and metabolism</topic><topic>Fatty acids</topic><topic>Firmicutes</topic><topic>Glutamate dehydrogenase</topic><topic>High protein diet</topic><topic>high‐beef‐protein diet</topic><topic>Inflammation</topic><topic>intestinal microbiota</topic><topic>Intestinal microflora</topic><topic>Intestine</topic><topic>Leaves</topic><topic>Metabolism</topic><topic>Microbiota</topic><topic>Microorganisms</topic><topic>mulberry leaf and konjac compound powder</topic><topic>old mice</topic><topic>Older people</topic><topic>Powder</topic><topic>Protein metabolism</topic><topic>Protein turnover</topic><topic>Proteins</topic><topic>Reverse transcription</topic><topic>rRNA 16S</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deng, Liling</creatorcontrib><creatorcontrib>Zhong, Geng</creatorcontrib><creatorcontrib>Peng, Yongbo</creatorcontrib><creatorcontrib>Zhu, Zhaojing</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the science of food and agriculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deng, Liling</au><au>Zhong, Geng</au><au>Peng, Yongbo</au><au>Zhu, Zhaojing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mulberry leaf and konjac compound powder improves the metabolic capacity of old mice on a high‐protein diet by regulating the structure of the intestinal microbiota</atitle><jtitle>Journal of the science of food and agriculture</jtitle><addtitle>J Sci Food Agric</addtitle><date>2023-09</date><risdate>2023</risdate><volume>103</volume><issue>12</issue><spage>5949</spage><epage>5957</epage><pages>5949-5957</pages><issn>0022-5142</issn><eissn>1097-0010</eissn><abstract>BACKGROUND
The health of elderly individuals is closely linked to their protein intake and the abundance of intestinal microbiota. To investigate the impact of a compound powder made from mulberry leaf and konjac (hereinafter referred to as ‘compound powder’) on regulating the structure of intestinal microbiota in 15‐month‐old BALB/c mice that were fed a high‐beef‐protein diet, 16S rRNA high‐throughput sequencing, reverse transcription quantitative polymerase reaction, western blot, and other biochemical methods were used to analyze the differences in intestinal microbiota, protein metabolism‐related genes, short‐chain fatty acid (SCFA) content, and serum cytokines.
RESULTS
The results showed that the compound powder increased the content of SCFAs, reduced the inflammatory reaction of the body, adjusted the abundance of intestinal microbiota (Firmicutes and Bacteroidetes), and increased the ratio of Firmicutes to Bacteroidetes (F/B). Moreover, the compound powder could increase the abundance of Lactobacillus and some non‐dominant bacteria that were related to amino acid metabolism and beneficial to human health, such as Eubacterium coprostanoligenes. These beneficial bacteria competitively reduced the abundance of harmful bacteria to protect the intestinal barrier and promote intestinal health, and upregulated the activities of aminopeptidase, proton‐coupled oligopeptide transporter 1, and glutamate dehydrogenase at the transcription and translation levels.
CONCLUSION
The compound powder could balance the abundance of intestinal microbiota, which may improve the metabolic capacity of old mice on a high‐protein diet, and ultimately promoting the well‐being of elderly individuals. © 2023 Society of Chemical Industry.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>37127926</pmid><doi>10.1002/jsfa.12664</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-6202-6108</orcidid></addata></record> |
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| subjects | Abundance Amino acids Aminopeptidase Bacteria Bacteroidetes Diet digestion and metabolism Fatty acids Firmicutes Glutamate dehydrogenase High protein diet high‐beef‐protein diet Inflammation intestinal microbiota Intestinal microflora Intestine Leaves Metabolism Microbiota Microorganisms mulberry leaf and konjac compound powder old mice Older people Powder Protein metabolism Protein turnover Proteins Reverse transcription rRNA 16S |
| title | Mulberry leaf and konjac compound powder improves the metabolic capacity of old mice on a high‐protein diet by regulating the structure of the intestinal microbiota |
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