Simultaneous Detection of Two Extracellular Vesicle Subpopulations in Saliva Assisting Tumor T Staging of Oral Squamous Cell Carcinoma
Extracellular vesicles (EVs), acting as important mediators of intercellular communication, play an essential role in physiological processes, which have unique potential in the medical field. However, the heterogeneity of EVs limits their development for disease diagnosis and therapy, making the EV...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2023-05, Vol.95 (19), p.7753-7760 |
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| creator | Feng, Jiao Xiao, Bo-Lin Zhang, Lin-Zhou Zhang, Yi-Hua Tang, Man Xu, Chun-Miao Chen, Gang Zhang, Zhi-Ling |
| description | Extracellular vesicles (EVs), acting as important mediators of intercellular communication, play an essential role in physiological processes, which have unique potential in the medical field. However, the heterogeneity of EVs limits their development for disease diagnosis and therapy, making the EV subpopulation analysis extremely valuable. In this article, a simple microfluidic approach was presented for the on-chip specific isolation and detection of two phenotypes of EVs (Annexin V+ EGFR+ EVs and Annexin V– EGFR+ EVs) based on different biomolecule-modified magnetic nanospheres and a fluorescence labeling technique. Combined with the control of the magnetic field in the microzone and fluid flow, it was easy to form two separate functional regions in the chip to capture different EV subpopulations. This method was successfully applied to the tests of clinical saliva samples in 75 oral squamous cell carcinoma (OSCC) patients and 10 healthy people. The results showed that the total level of EGFR+ EVs was much higher in OSCC patients that in healthy people. Meantime, the ratio of Annexin V+ EGFR+ EVs to Annexin V– EGFR+ EVs was found to be negatively correlated with tumor T stage of OSCC patients with a statistical difference, which suggested the ratio as a clinical index for monitoring the progression of OSCC in real time based on a noninvasive method. The approach provided a novel idea for evaluating the tumor T stage of OSCC and a powerful tool for clinical application. |
| doi_str_mv | 10.1021/acs.analchem.3c00940 |
| format | Article |
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However, the heterogeneity of EVs limits their development for disease diagnosis and therapy, making the EV subpopulation analysis extremely valuable. In this article, a simple microfluidic approach was presented for the on-chip specific isolation and detection of two phenotypes of EVs (Annexin V+ EGFR+ EVs and Annexin V– EGFR+ EVs) based on different biomolecule-modified magnetic nanospheres and a fluorescence labeling technique. Combined with the control of the magnetic field in the microzone and fluid flow, it was easy to form two separate functional regions in the chip to capture different EV subpopulations. This method was successfully applied to the tests of clinical saliva samples in 75 oral squamous cell carcinoma (OSCC) patients and 10 healthy people. The results showed that the total level of EGFR+ EVs was much higher in OSCC patients that in healthy people. Meantime, the ratio of Annexin V+ EGFR+ EVs to Annexin V– EGFR+ EVs was found to be negatively correlated with tumor T stage of OSCC patients with a statistical difference, which suggested the ratio as a clinical index for monitoring the progression of OSCC in real time based on a noninvasive method. The approach provided a novel idea for evaluating the tumor T stage of OSCC and a powerful tool for clinical application.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.3c00940</identifier><identifier>PMID: 37130010</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Annexin A5 ; Annexin V ; Biomolecules ; Carcinoma, Squamous Cell - diagnosis ; Carcinoma, Squamous Cell - pathology ; Cell interactions ; Chemistry ; Epidermal growth factor receptors ; ErbB Receptors - metabolism ; Extracellular Vesicles - metabolism ; Fluid flow ; Head and Neck Neoplasms - pathology ; Heterogeneity ; Humans ; Magnetic fields ; Microfluidics ; Mouth Neoplasms - diagnosis ; Mouth Neoplasms - pathology ; Nanospheres ; Oral cancer ; Oral carcinoma ; Oral squamous cell carcinoma ; Patients ; Phenotypes ; Saliva ; Saliva - metabolism ; Squamous cell carcinoma ; Squamous Cell Carcinoma of Head and Neck ; Subpopulations ; Tumors</subject><ispartof>Analytical chemistry (Washington), 2023-05, Vol.95 (19), p.7753-7760</ispartof><rights>2023 American Chemical Society</rights><rights>Copyright American Chemical Society May 16, 2023</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a376t-9df0d39eb7d7cd70b4c8a6ce12bf43a5adf552f99a45841027d92b47bda221a53</citedby><cites>FETCH-LOGICAL-a376t-9df0d39eb7d7cd70b4c8a6ce12bf43a5adf552f99a45841027d92b47bda221a53</cites><orcidid>0000-0003-3332-3511 ; 0000-0001-7807-2264</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.3c00940$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.3c00940$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27081,27929,27930,56743,56793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37130010$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Feng, Jiao</creatorcontrib><creatorcontrib>Xiao, Bo-Lin</creatorcontrib><creatorcontrib>Zhang, Lin-Zhou</creatorcontrib><creatorcontrib>Zhang, Yi-Hua</creatorcontrib><creatorcontrib>Tang, Man</creatorcontrib><creatorcontrib>Xu, Chun-Miao</creatorcontrib><creatorcontrib>Chen, Gang</creatorcontrib><creatorcontrib>Zhang, Zhi-Ling</creatorcontrib><title>Simultaneous Detection of Two Extracellular Vesicle Subpopulations in Saliva Assisting Tumor T Staging of Oral Squamous Cell Carcinoma</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Extracellular vesicles (EVs), acting as important mediators of intercellular communication, play an essential role in physiological processes, which have unique potential in the medical field. However, the heterogeneity of EVs limits their development for disease diagnosis and therapy, making the EV subpopulation analysis extremely valuable. In this article, a simple microfluidic approach was presented for the on-chip specific isolation and detection of two phenotypes of EVs (Annexin V+ EGFR+ EVs and Annexin V– EGFR+ EVs) based on different biomolecule-modified magnetic nanospheres and a fluorescence labeling technique. Combined with the control of the magnetic field in the microzone and fluid flow, it was easy to form two separate functional regions in the chip to capture different EV subpopulations. This method was successfully applied to the tests of clinical saliva samples in 75 oral squamous cell carcinoma (OSCC) patients and 10 healthy people. The results showed that the total level of EGFR+ EVs was much higher in OSCC patients that in healthy people. Meantime, the ratio of Annexin V+ EGFR+ EVs to Annexin V– EGFR+ EVs was found to be negatively correlated with tumor T stage of OSCC patients with a statistical difference, which suggested the ratio as a clinical index for monitoring the progression of OSCC in real time based on a noninvasive method. The approach provided a novel idea for evaluating the tumor T stage of OSCC and a powerful tool for clinical application.</description><subject>Annexin A5</subject><subject>Annexin V</subject><subject>Biomolecules</subject><subject>Carcinoma, Squamous Cell - diagnosis</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Cell interactions</subject><subject>Chemistry</subject><subject>Epidermal growth factor receptors</subject><subject>ErbB Receptors - metabolism</subject><subject>Extracellular Vesicles - metabolism</subject><subject>Fluid flow</subject><subject>Head and Neck Neoplasms - pathology</subject><subject>Heterogeneity</subject><subject>Humans</subject><subject>Magnetic fields</subject><subject>Microfluidics</subject><subject>Mouth Neoplasms - diagnosis</subject><subject>Mouth Neoplasms - pathology</subject><subject>Nanospheres</subject><subject>Oral cancer</subject><subject>Oral carcinoma</subject><subject>Oral squamous cell carcinoma</subject><subject>Patients</subject><subject>Phenotypes</subject><subject>Saliva</subject><subject>Saliva - metabolism</subject><subject>Squamous cell carcinoma</subject><subject>Squamous Cell Carcinoma of Head and Neck</subject><subject>Subpopulations</subject><subject>Tumors</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1O3DAUha2Kqkxp36BCltiwyfTajvOzRFOglZBYZNptdOM4YOTEgx235QV4bhzNwKKLrqxrfedc3XMI-cJgzYCzr6jCGie06l6Pa6EA6hzekRWTHLKiqvgRWQGAyHgJcEw-hvAAwBiw4gM5FiUTaYIVeW7MGO2Mk3Yx0G961mo2bqJuoNs_jl7-nT0qbW206OkvHYyymjax27ld-lrQQM1EG7TmN9KLEEyYzXRHt3F0nm5pM-PdMie_W4-WNo8Rx2XVJpnSDXplJjfiJ_J-QBv058N7Qn5eXW4337Ob2-sfm4ubDEVZzFndD9CLWndlX6q-hC5XFRZKM94NuUCJ_SAlH-oac1nlKaayr3mXl12PnDOU4oSc73133j1GHeZ2NGG5bx9AyyuoZCULLhJ69g_64KJPgS8Uk7KuhKwTle8p5V0IXg_tzpsR_VPLoF16alNP7WtP7aGnJDs9mMdu1P2b6LWYBMAeWORvi__r-QLGWqO2</recordid><startdate>20230516</startdate><enddate>20230516</enddate><creator>Feng, Jiao</creator><creator>Xiao, Bo-Lin</creator><creator>Zhang, Lin-Zhou</creator><creator>Zhang, Yi-Hua</creator><creator>Tang, Man</creator><creator>Xu, Chun-Miao</creator><creator>Chen, Gang</creator><creator>Zhang, Zhi-Ling</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3332-3511</orcidid><orcidid>https://orcid.org/0000-0001-7807-2264</orcidid></search><sort><creationdate>20230516</creationdate><title>Simultaneous Detection of Two Extracellular Vesicle Subpopulations in Saliva Assisting Tumor T Staging of Oral Squamous Cell Carcinoma</title><author>Feng, Jiao ; 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Chem</addtitle><date>2023-05-16</date><risdate>2023</risdate><volume>95</volume><issue>19</issue><spage>7753</spage><epage>7760</epage><pages>7753-7760</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>Extracellular vesicles (EVs), acting as important mediators of intercellular communication, play an essential role in physiological processes, which have unique potential in the medical field. However, the heterogeneity of EVs limits their development for disease diagnosis and therapy, making the EV subpopulation analysis extremely valuable. In this article, a simple microfluidic approach was presented for the on-chip specific isolation and detection of two phenotypes of EVs (Annexin V+ EGFR+ EVs and Annexin V– EGFR+ EVs) based on different biomolecule-modified magnetic nanospheres and a fluorescence labeling technique. Combined with the control of the magnetic field in the microzone and fluid flow, it was easy to form two separate functional regions in the chip to capture different EV subpopulations. This method was successfully applied to the tests of clinical saliva samples in 75 oral squamous cell carcinoma (OSCC) patients and 10 healthy people. The results showed that the total level of EGFR+ EVs was much higher in OSCC patients that in healthy people. Meantime, the ratio of Annexin V+ EGFR+ EVs to Annexin V– EGFR+ EVs was found to be negatively correlated with tumor T stage of OSCC patients with a statistical difference, which suggested the ratio as a clinical index for monitoring the progression of OSCC in real time based on a noninvasive method. The approach provided a novel idea for evaluating the tumor T stage of OSCC and a powerful tool for clinical application.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>37130010</pmid><doi>10.1021/acs.analchem.3c00940</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-3332-3511</orcidid><orcidid>https://orcid.org/0000-0001-7807-2264</orcidid></addata></record> |
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| subjects | Annexin A5 Annexin V Biomolecules Carcinoma, Squamous Cell - diagnosis Carcinoma, Squamous Cell - pathology Cell interactions Chemistry Epidermal growth factor receptors ErbB Receptors - metabolism Extracellular Vesicles - metabolism Fluid flow Head and Neck Neoplasms - pathology Heterogeneity Humans Magnetic fields Microfluidics Mouth Neoplasms - diagnosis Mouth Neoplasms - pathology Nanospheres Oral cancer Oral carcinoma Oral squamous cell carcinoma Patients Phenotypes Saliva Saliva - metabolism Squamous cell carcinoma Squamous Cell Carcinoma of Head and Neck Subpopulations Tumors |
| title | Simultaneous Detection of Two Extracellular Vesicle Subpopulations in Saliva Assisting Tumor T Staging of Oral Squamous Cell Carcinoma |
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