Microbes in fingerprints: A source for dating crime evidence?

Interest in the human microbiome has grown in recent years because of increasing applications to biomedicine and forensic science. However, the potential for dating evidence at a crime scene based upon time-dependent changes in microbial signatures has not been established, despite a relatively straightforward scientific process for isolating the microbiome. We hypothesize that modifications in microbial diversity, abundance, and succession can provide estimates of the time a surface was touched for investigative purposes. In this proof-of-concept research, the sequencing and analysis of the 16 S rRNA gene from microbes present in fresh and aged latent fingerprints deposited by three donors with pre- and post-washed hands is reported. The stability of major microbial phyla is confirmed while the dynamics of less abundant groups is described up to 21 days post-deposition. Most importantly, a phylum is suggested as the source for possible biological markers to date fingerprints: Deinococcus-Thermus. •The microbiome of latent fingerprints has been analyzed using 16 S rRNA sequencing.•It has been proven that major microbial groups remain almost intact up to 7 days post-deposition.•Microbial succession clearly occurs at 14 days post-deposition for minor phyla.•The phylum Deinococcus-Thermus has been identified as a marker to date latent fingerprints.