Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile
Seventy-five flea pools (one to ten fleas per pool) from 51 Andean foxes (Lycalopex culpaeus) and five South American grey foxes or chillas (Lycalopex griseus) from the Mediterranean region of Chile were analyzed for the presence of DNA of Bartonella spp. and Rickettsia spp. through quantitative rea...
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| creator | Millán, Javier Sepúlveda-García, Paulina Di Cataldo, Sophia Canales, Nivia Sallaberry-Pincheira, Nicole Painean, Javier Cevidanes, Aitor Müller, Ananda |
| description | Seventy-five flea pools (one to ten fleas per pool) from 51 Andean foxes (Lycalopex culpaeus) and five South American grey foxes or chillas (Lycalopex griseus) from the Mediterranean region of Chile were analyzed for the presence of DNA of Bartonella spp. and Rickettsia spp. through quantitative real-time PCR for the nouG and gltA genes, respectively. Positive samples were further characterized by conventional PCR protocols, targeting gltA and ITS genes for Bartonella, and gltA, ompA, and ompB genes for Rickettsia. Bartonella was detected in 48 % of the Pulex irritans pools (B. rochalimae in three pools, B. berkhoffii in two pools, B. henselae in one pool), and 8 % of the Ctenocephalides felis felis pools (B. rochalimae, one pool). Rickettsia was confirmed in 11 % of P. irritans pools and 92 % of the Ct. felis pools. Characterization confirmed R. felis in all sequenced Rickettsia-positive pools. All Ct. canis pools were negative. A Ct. felis pool from a wild-found domestic ferret (Mustela putorius furo) also resulted positive for R. felis. Although opportunistic, this survey provides the first description of zoonotic pathogens naturally circulating in fleas parasitizing Chilean free-living carnivores.
•Wild fox fleas from Chile analysed as pools for DNA of Bartonella and Rickettsia.•46 % and 11 % of Pulex irritans pools were positive for Bartonella and Rickettsia, respectively.•18 % and 85 % of Ctenocephalides felis felis pools were positive for Bartonella and Rickettsia, respectively.•Sequencing confirmed DNA of B. rochalimae, B. berkhoffii, B. henselae and R. felis.•All Ctenocephalides canis pools resulted negative. |
| doi_str_mv | 10.1016/j.cimid.2023.101983 |
| format | Article |
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•Wild fox fleas from Chile analysed as pools for DNA of Bartonella and Rickettsia.•46 % and 11 % of Pulex irritans pools were positive for Bartonella and Rickettsia, respectively.•18 % and 85 % of Ctenocephalides felis felis pools were positive for Bartonella and Rickettsia, respectively.•Sequencing confirmed DNA of B. rochalimae, B. berkhoffii, B. henselae and R. felis.•All Ctenocephalides canis pools resulted negative.</description><identifier>ISSN: 0147-9571</identifier><identifier>EISSN: 1878-1667</identifier><identifier>DOI: 10.1016/j.cimid.2023.101983</identifier><identifier>PMID: 37099997</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Bartonella - genetics ; Carnivora ; Chile - epidemiology ; Ctenocephalides - genetics ; Culpeo ; Dog Diseases - microbiology ; Dogs ; Ferrets - genetics ; Flea Infestations - epidemiology ; Flea Infestations - veterinary ; Flea-borne ; Foxes ; Mustelidae ; Pseudalopex ; Real-Time Polymerase Chain Reaction - veterinary ; Rickettsia - genetics ; Rickettsia felis - genetics ; Siphonaptera - microbiology ; South America ; Vector-borne</subject><ispartof>Comparative immunology, microbiology and infectious diseases, 2023-05, Vol.96, p.101983-101983, Article 101983</ispartof><rights>2023 The Authors</rights><rights>Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-5c7f70dc75ead01ad2204be4f8cb7bb0906c961745fa2448c810fa16a21c00703</citedby><cites>FETCH-LOGICAL-c404t-5c7f70dc75ead01ad2204be4f8cb7bb0906c961745fa2448c810fa16a21c00703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cimid.2023.101983$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37099997$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Millán, Javier</creatorcontrib><creatorcontrib>Sepúlveda-García, Paulina</creatorcontrib><creatorcontrib>Di Cataldo, Sophia</creatorcontrib><creatorcontrib>Canales, Nivia</creatorcontrib><creatorcontrib>Sallaberry-Pincheira, Nicole</creatorcontrib><creatorcontrib>Painean, Javier</creatorcontrib><creatorcontrib>Cevidanes, Aitor</creatorcontrib><creatorcontrib>Müller, Ananda</creatorcontrib><title>Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile</title><title>Comparative immunology, microbiology and infectious diseases</title><addtitle>Comp Immunol Microbiol Infect Dis</addtitle><description>Seventy-five flea pools (one to ten fleas per pool) from 51 Andean foxes (Lycalopex culpaeus) and five South American grey foxes or chillas (Lycalopex griseus) from the Mediterranean region of Chile were analyzed for the presence of DNA of Bartonella spp. and Rickettsia spp. through quantitative real-time PCR for the nouG and gltA genes, respectively. Positive samples were further characterized by conventional PCR protocols, targeting gltA and ITS genes for Bartonella, and gltA, ompA, and ompB genes for Rickettsia. Bartonella was detected in 48 % of the Pulex irritans pools (B. rochalimae in three pools, B. berkhoffii in two pools, B. henselae in one pool), and 8 % of the Ctenocephalides felis felis pools (B. rochalimae, one pool). Rickettsia was confirmed in 11 % of P. irritans pools and 92 % of the Ct. felis pools. Characterization confirmed R. felis in all sequenced Rickettsia-positive pools. All Ct. canis pools were negative. A Ct. felis pool from a wild-found domestic ferret (Mustela putorius furo) also resulted positive for R. felis. Although opportunistic, this survey provides the first description of zoonotic pathogens naturally circulating in fleas parasitizing Chilean free-living carnivores.
•Wild fox fleas from Chile analysed as pools for DNA of Bartonella and Rickettsia.•46 % and 11 % of Pulex irritans pools were positive for Bartonella and Rickettsia, respectively.•18 % and 85 % of Ctenocephalides felis felis pools were positive for Bartonella and Rickettsia, respectively.•Sequencing confirmed DNA of B. rochalimae, B. berkhoffii, B. henselae and R. felis.•All Ctenocephalides canis pools resulted negative.</description><subject>Animals</subject><subject>Bartonella - genetics</subject><subject>Carnivora</subject><subject>Chile - epidemiology</subject><subject>Ctenocephalides - genetics</subject><subject>Culpeo</subject><subject>Dog Diseases - microbiology</subject><subject>Dogs</subject><subject>Ferrets - genetics</subject><subject>Flea Infestations - epidemiology</subject><subject>Flea Infestations - veterinary</subject><subject>Flea-borne</subject><subject>Foxes</subject><subject>Mustelidae</subject><subject>Pseudalopex</subject><subject>Real-Time Polymerase Chain Reaction - veterinary</subject><subject>Rickettsia - genetics</subject><subject>Rickettsia felis - genetics</subject><subject>Siphonaptera - microbiology</subject><subject>South America</subject><subject>Vector-borne</subject><issn>0147-9571</issn><issn>1878-1667</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1PwzAMhiMEgvHxC5BQjhzocNqsaQ8cYOJLAiEhOEdp4giPrhlJh-Df0zHgiC-WrPf1az-MHQoYCxDl6WxsaU5unENerCZ1VWywkahUlYmyVJtsBEKqrJ4oscN2U5oBQC2k2GY7hYJ6KDViT_ehRbtsTeTksOvJkzU9hY4Hzy9M7EOHbWt4WizG3HSOP5J9xb5PZLjHlhKnjvvwwX2LJp3w6Qu1uM-2vGkTHvz0PfZ8dfk0vcnuHq5vp-d3mZUg-2xilVfgrJqgcSCMy3OQDUpf2UY1DdRQ2roUSk68yaWsbCXAG1GaXFgABcUeO17vXcTwtsTU6zklu7q3w7BMOq-grOuyqNQgLdZSG0NKEb1eRJqb-KkF6BVOPdPfOPUKp17jHFxHPwHLZo7uz_PLbxCcrQU4vPlOGHWyhJ1FRxFtr12gfwO-ABi1hms</recordid><startdate>202305</startdate><enddate>202305</enddate><creator>Millán, Javier</creator><creator>Sepúlveda-García, Paulina</creator><creator>Di Cataldo, Sophia</creator><creator>Canales, Nivia</creator><creator>Sallaberry-Pincheira, Nicole</creator><creator>Painean, Javier</creator><creator>Cevidanes, Aitor</creator><creator>Müller, Ananda</creator><general>Elsevier Ltd</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202305</creationdate><title>Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile</title><author>Millán, Javier ; Sepúlveda-García, Paulina ; Di Cataldo, Sophia ; Canales, Nivia ; Sallaberry-Pincheira, Nicole ; Painean, Javier ; Cevidanes, Aitor ; Müller, Ananda</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-5c7f70dc75ead01ad2204be4f8cb7bb0906c961745fa2448c810fa16a21c00703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Bartonella - genetics</topic><topic>Carnivora</topic><topic>Chile - epidemiology</topic><topic>Ctenocephalides - genetics</topic><topic>Culpeo</topic><topic>Dog Diseases - microbiology</topic><topic>Dogs</topic><topic>Ferrets - genetics</topic><topic>Flea Infestations - epidemiology</topic><topic>Flea Infestations - veterinary</topic><topic>Flea-borne</topic><topic>Foxes</topic><topic>Mustelidae</topic><topic>Pseudalopex</topic><topic>Real-Time Polymerase Chain Reaction - veterinary</topic><topic>Rickettsia - genetics</topic><topic>Rickettsia felis - genetics</topic><topic>Siphonaptera - microbiology</topic><topic>South America</topic><topic>Vector-borne</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Millán, Javier</creatorcontrib><creatorcontrib>Sepúlveda-García, Paulina</creatorcontrib><creatorcontrib>Di Cataldo, Sophia</creatorcontrib><creatorcontrib>Canales, Nivia</creatorcontrib><creatorcontrib>Sallaberry-Pincheira, Nicole</creatorcontrib><creatorcontrib>Painean, Javier</creatorcontrib><creatorcontrib>Cevidanes, Aitor</creatorcontrib><creatorcontrib>Müller, Ananda</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Comparative immunology, microbiology and infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Millán, Javier</au><au>Sepúlveda-García, Paulina</au><au>Di Cataldo, Sophia</au><au>Canales, Nivia</au><au>Sallaberry-Pincheira, Nicole</au><au>Painean, Javier</au><au>Cevidanes, Aitor</au><au>Müller, Ananda</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile</atitle><jtitle>Comparative immunology, microbiology and infectious diseases</jtitle><addtitle>Comp Immunol Microbiol Infect Dis</addtitle><date>2023-05</date><risdate>2023</risdate><volume>96</volume><spage>101983</spage><epage>101983</epage><pages>101983-101983</pages><artnum>101983</artnum><issn>0147-9571</issn><eissn>1878-1667</eissn><abstract>Seventy-five flea pools (one to ten fleas per pool) from 51 Andean foxes (Lycalopex culpaeus) and five South American grey foxes or chillas (Lycalopex griseus) from the Mediterranean region of Chile were analyzed for the presence of DNA of Bartonella spp. and Rickettsia spp. through quantitative real-time PCR for the nouG and gltA genes, respectively. Positive samples were further characterized by conventional PCR protocols, targeting gltA and ITS genes for Bartonella, and gltA, ompA, and ompB genes for Rickettsia. Bartonella was detected in 48 % of the Pulex irritans pools (B. rochalimae in three pools, B. berkhoffii in two pools, B. henselae in one pool), and 8 % of the Ctenocephalides felis felis pools (B. rochalimae, one pool). Rickettsia was confirmed in 11 % of P. irritans pools and 92 % of the Ct. felis pools. Characterization confirmed R. felis in all sequenced Rickettsia-positive pools. All Ct. canis pools were negative. A Ct. felis pool from a wild-found domestic ferret (Mustela putorius furo) also resulted positive for R. felis. Although opportunistic, this survey provides the first description of zoonotic pathogens naturally circulating in fleas parasitizing Chilean free-living carnivores.
•Wild fox fleas from Chile analysed as pools for DNA of Bartonella and Rickettsia.•46 % and 11 % of Pulex irritans pools were positive for Bartonella and Rickettsia, respectively.•18 % and 85 % of Ctenocephalides felis felis pools were positive for Bartonella and Rickettsia, respectively.•Sequencing confirmed DNA of B. rochalimae, B. berkhoffii, B. henselae and R. felis.•All Ctenocephalides canis pools resulted negative.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>37099997</pmid><doi>10.1016/j.cimid.2023.101983</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Comparative immunology, microbiology and infectious diseases, 2023-05, Vol.96, p.101983-101983, Article 101983 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Bartonella - genetics Carnivora Chile - epidemiology Ctenocephalides - genetics Culpeo Dog Diseases - microbiology Dogs Ferrets - genetics Flea Infestations - epidemiology Flea Infestations - veterinary Flea-borne Foxes Mustelidae Pseudalopex Real-Time Polymerase Chain Reaction - veterinary Rickettsia - genetics Rickettsia felis - genetics Siphonaptera - microbiology South America Vector-borne |
| title | Molecular identification of Bartonella spp. and Rickettsia felis in fox fleas, Chile |
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