Expression profiles of the CD274 and PLEKHH2 gene and association of its polymorphism with hematologic parameters in sheep
CD274 and PLEKHH2 genes have been identified as immune- and multiple diseases-related genes, and have recently garnered significant interest. However, their role in regulating immune functions in sheep remains largely unexplored. In this study, we aimed to investigate the effects of polymorphisms in...
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| Veröffentlicht in: | Veterinary immunology and immunopathology 2023-05, Vol.259, p.110597-110597, Article 110597 |
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| container_title | Veterinary immunology and immunopathology |
| container_volume | 259 |
| creator | Zhang, Yukun Zhang, Xiaoxue Li, Fadi Lin, Changchun Zhang, Deyin Duan, Benzhen Zhao, Yuan Li, Xiaolong Xu, Dan Cheng, Jiangbo Zhao, Liming Wang, Jianghui Wang, Weimin |
| description | CD274 and PLEKHH2 genes have been identified as immune- and multiple diseases-related genes, and have recently garnered significant interest. However, their role in regulating immune functions in sheep remains largely unexplored. In this study, we aimed to investigate the effects of polymorphisms in CD274 and PLEKHH2 on hematologic parameters in 915 sheep. Our results showed that the CD274 and PLEKHH2 genes were most highly expressed in the spleen and tail fat, respectively, as determined by qRT-PCR. We also identified a G to A mutation (g 0.11858 G > A) in the exon 4 region of CD274, and a C to G mutation (g 0.38384 C > G) in the intron 8 region of PLEKH2. Association analysis revealed that CD274 g 0.11858 G > A was significantly associated with RBC, HCT, MCHC, and MCV (P G was significantly associated with HCT, MPV, MCHC, and MCV (P |
| doi_str_mv | 10.1016/j.vetimm.2023.110597 |
| format | Article |
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•The SNPs of CD274 and PLEKHH2 genes were associated with hematologic parameters in sheep.•Combined genotypes of CD274 and PLEKHH2 genes were associated with hematologic parameters in sheep.•The ovine CD274 and PLEKHH2 genes had the highest mRNA expression in the spleen and tail fat, respectively.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/j.vetimm.2023.110597</identifier><identifier>PMID: 37094535</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; CD274 ; Exons ; Hematologic parameters ; Immunity ; Phenotype ; PLEKHH2 ; Polymerase Chain Reaction - veterinary ; Polymorphism, Single Nucleotide ; Sheep ; Sheep - genetics</subject><ispartof>Veterinary immunology and immunopathology, 2023-05, Vol.259, p.110597-110597, Article 110597</ispartof><rights>2023 Elsevier B.V.</rights><rights>Copyright © 2023 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c311t-6175a12dc7e0159fe12eb524dbc4f7695b5d9ab6e79260d7bd139ea468620613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetimm.2023.110597$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37094535$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Yukun</creatorcontrib><creatorcontrib>Zhang, Xiaoxue</creatorcontrib><creatorcontrib>Li, Fadi</creatorcontrib><creatorcontrib>Lin, Changchun</creatorcontrib><creatorcontrib>Zhang, Deyin</creatorcontrib><creatorcontrib>Duan, Benzhen</creatorcontrib><creatorcontrib>Zhao, Yuan</creatorcontrib><creatorcontrib>Li, Xiaolong</creatorcontrib><creatorcontrib>Xu, Dan</creatorcontrib><creatorcontrib>Cheng, Jiangbo</creatorcontrib><creatorcontrib>Zhao, Liming</creatorcontrib><creatorcontrib>Wang, Jianghui</creatorcontrib><creatorcontrib>Wang, Weimin</creatorcontrib><title>Expression profiles of the CD274 and PLEKHH2 gene and association of its polymorphism with hematologic parameters in sheep</title><title>Veterinary immunology and immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>CD274 and PLEKHH2 genes have been identified as immune- and multiple diseases-related genes, and have recently garnered significant interest. However, their role in regulating immune functions in sheep remains largely unexplored. In this study, we aimed to investigate the effects of polymorphisms in CD274 and PLEKHH2 on hematologic parameters in 915 sheep. Our results showed that the CD274 and PLEKHH2 genes were most highly expressed in the spleen and tail fat, respectively, as determined by qRT-PCR. We also identified a G to A mutation (g 0.11858 G > A) in the exon 4 region of CD274, and a C to G mutation (g 0.38384 C > G) in the intron 8 region of PLEKH2. Association analysis revealed that CD274 g 0.11858 G > A was significantly associated with RBC, HCT, MCHC, and MCV (P < 0.05), while PLEKHH2 g 0.38384 C > G was significantly associated with HCT, MPV, MCHC, and MCV (P < 0.05). These results suggest that CD274 and PLEKHH2 genes may play a role in regulating blood physiological indicators and could be potential functional candidates for influencing immune traits in sheep breeding programs.
•The SNPs of CD274 and PLEKHH2 genes were associated with hematologic parameters in sheep.•Combined genotypes of CD274 and PLEKHH2 genes were associated with hematologic parameters in sheep.•The ovine CD274 and PLEKHH2 genes had the highest mRNA expression in the spleen and tail fat, respectively.</description><subject>Animals</subject><subject>CD274</subject><subject>Exons</subject><subject>Hematologic parameters</subject><subject>Immunity</subject><subject>Phenotype</subject><subject>PLEKHH2</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Sheep</subject><subject>Sheep - genetics</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMGO0zAQhi0EYsvCGyDkI5cU24nt5IKESqGISnDYu-XYk42rOA62u8vy9Lhk4chppNH3z6_5EHpNyZYSKt6dtneQnfdbRli9pZTwTj5BG9rKumK8bp6iTcF4xRomr9CLlE6EFKZtn6OrWpKu4TXfoF_7n0uElFyY8RLD4CZIOAw4j4B3H5lssJ4t_n7cfz0cGL6FGf4sdErBOJ0vsUK7nPASpgcf4jK65PG9yyMewescpnDrDF501B4yxITdjNMIsLxEzwY9JXj1OK_Rzaf9ze5QHb99_rL7cKxMTWmuBJVcU2aNBEJ5NwBl0HPW2N40gxQd77ntdC9AdkwQK3tL6w50I1rBiKD1NXq7ni3v_ThDysq7ZGCa9AzhnBRriSCSFVcFbVbUxJBShEEt0XkdHxQl6iJdndQqXV2kq1V6ib15bDj3Huy_0F_LBXi_AlDevHMQVTIOZgPWRTBZ2eD-3_Ab2_uVCA</recordid><startdate>202305</startdate><enddate>202305</enddate><creator>Zhang, Yukun</creator><creator>Zhang, Xiaoxue</creator><creator>Li, Fadi</creator><creator>Lin, Changchun</creator><creator>Zhang, Deyin</creator><creator>Duan, Benzhen</creator><creator>Zhao, Yuan</creator><creator>Li, Xiaolong</creator><creator>Xu, Dan</creator><creator>Cheng, Jiangbo</creator><creator>Zhao, Liming</creator><creator>Wang, Jianghui</creator><creator>Wang, Weimin</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202305</creationdate><title>Expression profiles of the CD274 and PLEKHH2 gene and association of its polymorphism with hematologic parameters in sheep</title><author>Zhang, Yukun ; Zhang, Xiaoxue ; Li, Fadi ; Lin, Changchun ; Zhang, Deyin ; Duan, Benzhen ; Zhao, Yuan ; Li, Xiaolong ; Xu, Dan ; Cheng, Jiangbo ; Zhao, Liming ; Wang, Jianghui ; Wang, Weimin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c311t-6175a12dc7e0159fe12eb524dbc4f7695b5d9ab6e79260d7bd139ea468620613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>CD274</topic><topic>Exons</topic><topic>Hematologic parameters</topic><topic>Immunity</topic><topic>Phenotype</topic><topic>PLEKHH2</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Sheep</topic><topic>Sheep - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Yukun</creatorcontrib><creatorcontrib>Zhang, Xiaoxue</creatorcontrib><creatorcontrib>Li, Fadi</creatorcontrib><creatorcontrib>Lin, Changchun</creatorcontrib><creatorcontrib>Zhang, Deyin</creatorcontrib><creatorcontrib>Duan, Benzhen</creatorcontrib><creatorcontrib>Zhao, Yuan</creatorcontrib><creatorcontrib>Li, Xiaolong</creatorcontrib><creatorcontrib>Xu, Dan</creatorcontrib><creatorcontrib>Cheng, Jiangbo</creatorcontrib><creatorcontrib>Zhao, Liming</creatorcontrib><creatorcontrib>Wang, Jianghui</creatorcontrib><creatorcontrib>Wang, Weimin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Yukun</au><au>Zhang, Xiaoxue</au><au>Li, Fadi</au><au>Lin, Changchun</au><au>Zhang, Deyin</au><au>Duan, Benzhen</au><au>Zhao, Yuan</au><au>Li, Xiaolong</au><au>Xu, Dan</au><au>Cheng, Jiangbo</au><au>Zhao, Liming</au><au>Wang, Jianghui</au><au>Wang, Weimin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression profiles of the CD274 and PLEKHH2 gene and association of its polymorphism with hematologic parameters in sheep</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>2023-05</date><risdate>2023</risdate><volume>259</volume><spage>110597</spage><epage>110597</epage><pages>110597-110597</pages><artnum>110597</artnum><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>CD274 and PLEKHH2 genes have been identified as immune- and multiple diseases-related genes, and have recently garnered significant interest. However, their role in regulating immune functions in sheep remains largely unexplored. In this study, we aimed to investigate the effects of polymorphisms in CD274 and PLEKHH2 on hematologic parameters in 915 sheep. Our results showed that the CD274 and PLEKHH2 genes were most highly expressed in the spleen and tail fat, respectively, as determined by qRT-PCR. We also identified a G to A mutation (g 0.11858 G > A) in the exon 4 region of CD274, and a C to G mutation (g 0.38384 C > G) in the intron 8 region of PLEKH2. Association analysis revealed that CD274 g 0.11858 G > A was significantly associated with RBC, HCT, MCHC, and MCV (P < 0.05), while PLEKHH2 g 0.38384 C > G was significantly associated with HCT, MPV, MCHC, and MCV (P < 0.05). These results suggest that CD274 and PLEKHH2 genes may play a role in regulating blood physiological indicators and could be potential functional candidates for influencing immune traits in sheep breeding programs.
•The SNPs of CD274 and PLEKHH2 genes were associated with hematologic parameters in sheep.•Combined genotypes of CD274 and PLEKHH2 genes were associated with hematologic parameters in sheep.•The ovine CD274 and PLEKHH2 genes had the highest mRNA expression in the spleen and tail fat, respectively.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>37094535</pmid><doi>10.1016/j.vetimm.2023.110597</doi><tpages>1</tpages></addata></record> |
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| subjects | Animals CD274 Exons Hematologic parameters Immunity Phenotype PLEKHH2 Polymerase Chain Reaction - veterinary Polymorphism, Single Nucleotide Sheep Sheep - genetics |
| title | Expression profiles of the CD274 and PLEKHH2 gene and association of its polymorphism with hematologic parameters in sheep |
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