Evaluating the effect of glycation on lipase activity using boronate affinity chromatography and mass spectrometry

[Display omitted] •BAC allowed enrichment of glycated proteoforms while maintaining lipase activity.•BAC with MS and activity assays to establish structure–function relationships.•Glycation does not reduce lipase activity and stability. Protein glycation may occur naturally when reducing sugars and...

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Veröffentlicht in:	Food chemistry 2023-09, Vol.421, p.136147-136147, Article 136147
Hauptverfasser:	van Schaick, Guusje, Pot, Sanne, Schouten, Olaf, den Hartog, Joost, Akeroyd, Michiel, van der Hoeven, Rob, Bijleveld, Wim, Abello, Nicolas, Wuhrer, Manfred, Olsthoorn, Maurien, Dominguez-Vega, Elena
Format:	Artikel
Sprache:	eng
Schlagworte:	Boronate affinity chromatography Chromatography, Affinity - methods Glycation Lipase Maillard Reaction Mass spectrometry Mass Spectrometry - methods Structure–function relationship Sugars
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