In vitro and ex vivo characterization of nanonized amniotic membrane particles: An untapped modality for ocular surface reconstruction
The pristine Human Amniotic Membrane (HAM) has portrayed outstanding potential as scaffold for ocular surface reconstruction and regeneration. However, in treatment procedures where the supporting membrane matrix of HAM is not obligatory and only the bioactive molecules are vital, the surgical pract...
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| Veröffentlicht in: | Experimental eye research 2023-06, Vol.231, p.109471-109471, Article 109471 |
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| creator | Thada, Raja Rajeshwari Debata, Mayadhar Mandal, Shuvam Gunasekaran, Deebasuganya Mohan, Vimala Devi Chandrasekaran, Niranjana Sivagnanam, Uma Tiruchirapalli |
| description | The pristine Human Amniotic Membrane (HAM) has portrayed outstanding potential as scaffold for ocular surface reconstruction and regeneration. However, in treatment procedures where the supporting membrane matrix of HAM is not obligatory and only the bioactive molecules are vital, the surgical practise of HAM grafting causes redundant trauma and economic burden to the patient. Hence, in our laboratory we have attempted to break down HAM to nanoscale particles and validate its potential as a competent ocular therapeutic agent; by conducting a comparative analysis between the fresh, lyophilized, micronized and Nanonized Amniotic Membrane (NAM) particles. Our results evidently showcased that the prepared NAM particles was |
| doi_str_mv | 10.1016/j.exer.2023.109471 |
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[Display omitted]
•The prepared Nanonized Amniotic Membrane (NAM) particles reported an average size of 75 nm.•The total collagen, collagen type I and IV were well retained; while hyaluronic acid was significantly elevated in NAM.•The NAM particles eluted higher amounts of proteins and growth factors when compared to pristine amniotic membrane.•The in vitro analyses of NAM displayed good biocompatibility and cell proliferating capabilities.•The ex vivo experiments demonstrated the ability of NAM to serve as an improved therapeutic agent for ocular treatments.</description><identifier>ISSN: 0014-4835</identifier><identifier>EISSN: 1096-0007</identifier><identifier>DOI: 10.1016/j.exer.2023.109471</identifier><identifier>PMID: 37086963</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Amnion ; Animals ; Collagen ; Cornea ; Cryomilling ; Ex vivo study ; Growth factors ; Human amniotic membrane ; Humans ; Microarray ; Nanonization ; Rabbits</subject><ispartof>Experimental eye research, 2023-06, Vol.231, p.109471-109471, Article 109471</ispartof><rights>2023 Elsevier Ltd</rights><rights>Copyright © 2023 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-a95919648c3d7bb85e36a96dfb12ffde5324d4b1862cb380c8e3e663c17ac1883</citedby><cites>FETCH-LOGICAL-c356t-a95919648c3d7bb85e36a96dfb12ffde5324d4b1862cb380c8e3e663c17ac1883</cites><orcidid>0000-0002-1401-2569</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.exer.2023.109471$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37086963$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thada, Raja Rajeshwari</creatorcontrib><creatorcontrib>Debata, Mayadhar</creatorcontrib><creatorcontrib>Mandal, Shuvam</creatorcontrib><creatorcontrib>Gunasekaran, Deebasuganya</creatorcontrib><creatorcontrib>Mohan, Vimala Devi</creatorcontrib><creatorcontrib>Chandrasekaran, Niranjana</creatorcontrib><creatorcontrib>Sivagnanam, Uma Tiruchirapalli</creatorcontrib><title>In vitro and ex vivo characterization of nanonized amniotic membrane particles: An untapped modality for ocular surface reconstruction</title><title>Experimental eye research</title><addtitle>Exp Eye Res</addtitle><description>The pristine Human Amniotic Membrane (HAM) has portrayed outstanding potential as scaffold for ocular surface reconstruction and regeneration. However, in treatment procedures where the supporting membrane matrix of HAM is not obligatory and only the bioactive molecules are vital, the surgical practise of HAM grafting causes redundant trauma and economic burden to the patient. Hence, in our laboratory we have attempted to break down HAM to nanoscale particles and validate its potential as a competent ocular therapeutic agent; by conducting a comparative analysis between the fresh, lyophilized, micronized and Nanonized Amniotic Membrane (NAM) particles. Our results evidently showcased that the prepared NAM particles was <100 nm and the major biomolecules such as collagen and hyaluronic acid were well retained. Further, the NAM particles eluted significantly higher amounts of proteins and growth factors while maintaining its stability and isotonicity when stored at 4 °C. Its biostability was assayed in the presence of lysozyme enzyme. Its remarkable ability to promote cell proliferation in rabbit corneal cells and negative cytotoxicity is an added advantage for ocular application. The ocular biocompatibility of NAM, evaluated by the ex vivo assessment of corneal thickness, transparency, histopathology, immunohistochemistry and corneal permeability clearly indicated its suitability for ophthalmic applications.
[Display omitted]
•The prepared Nanonized Amniotic Membrane (NAM) particles reported an average size of 75 nm.•The total collagen, collagen type I and IV were well retained; while hyaluronic acid was significantly elevated in NAM.•The NAM particles eluted higher amounts of proteins and growth factors when compared to pristine amniotic membrane.•The in vitro analyses of NAM displayed good biocompatibility and cell proliferating capabilities.•The ex vivo experiments demonstrated the ability of NAM to serve as an improved therapeutic agent for ocular treatments.</description><subject>Amnion</subject><subject>Animals</subject><subject>Collagen</subject><subject>Cornea</subject><subject>Cryomilling</subject><subject>Ex vivo study</subject><subject>Growth factors</subject><subject>Human amniotic membrane</subject><subject>Humans</subject><subject>Microarray</subject><subject>Nanonization</subject><subject>Rabbits</subject><issn>0014-4835</issn><issn>1096-0007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1u1DAUhS1ERYeBF2CBvGSTwY4Tx0FsqgpopUps2rV1Y98IjxI72M6o7QP0uevRFJZd3R-dc67uR8gnznaccfl1v8N7jLua1aIs-qbjb8imNLJijHVvyYYx3lSNEu05eZ_SvmxF0zXvyLnomJK9FBvydO3pweUYKHhL8b4Mh0DNH4hgMkb3CNkFT8NIPfjg3SNaCrN3ITtDZ5yHCB7pArHME6Zv9MLT1WdYliKcg4XJ5Qc6hkiDWSeINK1xBIM0ogk-5bia44EP5GyEKeHHl7oldz9_3F5eVTe_f11fXtxURrQyV9C3Pe9lo4yw3TCoFoWEXtpx4PU4WmxF3dhm4ErWZhCKGYUCpRSGd2C4UmJLvpxylxj-rpiynl0yOE3li7AmXSvWsrpti2dL6pPUxJBSxFEv0c0QHzRn-shf7_WRvz7y1yf-xfT5JX8dZrT_Lf-AF8H3kwDLlwdX7Mk49AatK0SytsG9lv8M2U6Zxg</recordid><startdate>202306</startdate><enddate>202306</enddate><creator>Thada, Raja Rajeshwari</creator><creator>Debata, Mayadhar</creator><creator>Mandal, Shuvam</creator><creator>Gunasekaran, Deebasuganya</creator><creator>Mohan, Vimala Devi</creator><creator>Chandrasekaran, Niranjana</creator><creator>Sivagnanam, Uma Tiruchirapalli</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1401-2569</orcidid></search><sort><creationdate>202306</creationdate><title>In vitro and ex vivo characterization of nanonized amniotic membrane particles: An untapped modality for ocular surface reconstruction</title><author>Thada, Raja Rajeshwari ; Debata, Mayadhar ; Mandal, Shuvam ; Gunasekaran, Deebasuganya ; Mohan, Vimala Devi ; Chandrasekaran, Niranjana ; Sivagnanam, Uma Tiruchirapalli</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-a95919648c3d7bb85e36a96dfb12ffde5324d4b1862cb380c8e3e663c17ac1883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Amnion</topic><topic>Animals</topic><topic>Collagen</topic><topic>Cornea</topic><topic>Cryomilling</topic><topic>Ex vivo study</topic><topic>Growth factors</topic><topic>Human amniotic membrane</topic><topic>Humans</topic><topic>Microarray</topic><topic>Nanonization</topic><topic>Rabbits</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thada, Raja Rajeshwari</creatorcontrib><creatorcontrib>Debata, Mayadhar</creatorcontrib><creatorcontrib>Mandal, Shuvam</creatorcontrib><creatorcontrib>Gunasekaran, Deebasuganya</creatorcontrib><creatorcontrib>Mohan, Vimala Devi</creatorcontrib><creatorcontrib>Chandrasekaran, Niranjana</creatorcontrib><creatorcontrib>Sivagnanam, Uma Tiruchirapalli</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thada, Raja Rajeshwari</au><au>Debata, Mayadhar</au><au>Mandal, Shuvam</au><au>Gunasekaran, Deebasuganya</au><au>Mohan, Vimala Devi</au><au>Chandrasekaran, Niranjana</au><au>Sivagnanam, Uma Tiruchirapalli</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro and ex vivo characterization of nanonized amniotic membrane particles: An untapped modality for ocular surface reconstruction</atitle><jtitle>Experimental eye research</jtitle><addtitle>Exp Eye Res</addtitle><date>2023-06</date><risdate>2023</risdate><volume>231</volume><spage>109471</spage><epage>109471</epage><pages>109471-109471</pages><artnum>109471</artnum><issn>0014-4835</issn><eissn>1096-0007</eissn><abstract>The pristine Human Amniotic Membrane (HAM) has portrayed outstanding potential as scaffold for ocular surface reconstruction and regeneration. However, in treatment procedures where the supporting membrane matrix of HAM is not obligatory and only the bioactive molecules are vital, the surgical practise of HAM grafting causes redundant trauma and economic burden to the patient. Hence, in our laboratory we have attempted to break down HAM to nanoscale particles and validate its potential as a competent ocular therapeutic agent; by conducting a comparative analysis between the fresh, lyophilized, micronized and Nanonized Amniotic Membrane (NAM) particles. Our results evidently showcased that the prepared NAM particles was <100 nm and the major biomolecules such as collagen and hyaluronic acid were well retained. Further, the NAM particles eluted significantly higher amounts of proteins and growth factors while maintaining its stability and isotonicity when stored at 4 °C. Its biostability was assayed in the presence of lysozyme enzyme. Its remarkable ability to promote cell proliferation in rabbit corneal cells and negative cytotoxicity is an added advantage for ocular application. The ocular biocompatibility of NAM, evaluated by the ex vivo assessment of corneal thickness, transparency, histopathology, immunohistochemistry and corneal permeability clearly indicated its suitability for ophthalmic applications.
[Display omitted]
•The prepared Nanonized Amniotic Membrane (NAM) particles reported an average size of 75 nm.•The total collagen, collagen type I and IV were well retained; while hyaluronic acid was significantly elevated in NAM.•The NAM particles eluted higher amounts of proteins and growth factors when compared to pristine amniotic membrane.•The in vitro analyses of NAM displayed good biocompatibility and cell proliferating capabilities.•The ex vivo experiments demonstrated the ability of NAM to serve as an improved therapeutic agent for ocular treatments.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>37086963</pmid><doi>10.1016/j.exer.2023.109471</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-1401-2569</orcidid></addata></record> |
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| subjects | Amnion Animals Collagen Cornea Cryomilling Ex vivo study Growth factors Human amniotic membrane Humans Microarray Nanonization Rabbits |
| title | In vitro and ex vivo characterization of nanonized amniotic membrane particles: An untapped modality for ocular surface reconstruction |
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