BmCPV replication is suppressed by the activation of the NF-κB/autophagy pathway through the interaction of vsp21 translated by vcircRNA_000048 with ubiquitin carboxyl-terminal hydrolase

Bombyx mori cypovirus (BmCPV), a typical double-stranded RNA virus, was demonstrated to generate a viral circRNA, vcircRNA_000048, which encodes a vsp21 with 21 amino acid residues to suppress viral replication. However, the regulatory mechanism of vsp21 on virus infection remained unclear. This stu...

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Veröffentlicht in:Insect biochemistry and molecular biology 2023-05, Vol.156, p.103947-103947, Article 103947
Hauptverfasser: Zhang, Yunshan, Zhu, Min, Pan, Jun, Qiu, Qunnan, Tong, Xinyu, Hu, Xiaolong, Gong, Chengliang
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container_title Insect biochemistry and molecular biology
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Zhu, Min
Pan, Jun
Qiu, Qunnan
Tong, Xinyu
Hu, Xiaolong
Gong, Chengliang
description Bombyx mori cypovirus (BmCPV), a typical double-stranded RNA virus, was demonstrated to generate a viral circRNA, vcircRNA_000048, which encodes a vsp21 with 21 amino acid residues to suppress viral replication. However, the regulatory mechanism of vsp21 on virus infection remained unclear. This study discovered that vsp21 induces reactive oxygen species (ROS) generation, activates autophagy, and attenuates virus replication by inducing autophagy. Then we confirmed that the effect of vsp21-induced autophagy on viral replication was attributed to the activation of the NF-κB signaling pathway. Furthermore, we clarified that vsp21 interacted with ubiquitin carboxyl-terminal hydrolase (UCH) and that ubiquitination and degradation of phospho-IκB-α were enhanced by vsp21 via competitive binding to UCH. Finally, we validated that vsp21 activates the NF-κB/autophagy pathway to suppress viral replication by interacting with UCH. These findings provided new insights into regulating viral multiplication and reovirus-host interaction. [Display omitted] •BmCPV was demonstrated to generate a viral circRNA, vcircRNA_000048, which encodes a vsp21 to suppress viral replication.•vsp21 induces ROS generation, activates autophagy, and attenuates virus replication by inducing autophagy.•vsp21 activates the NF-κB/autophagy pathway to suppress viral replication by interacting with UCH.
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However, the regulatory mechanism of vsp21 on virus infection remained unclear. This study discovered that vsp21 induces reactive oxygen species (ROS) generation, activates autophagy, and attenuates virus replication by inducing autophagy. Then we confirmed that the effect of vsp21-induced autophagy on viral replication was attributed to the activation of the NF-κB signaling pathway. Furthermore, we clarified that vsp21 interacted with ubiquitin carboxyl-terminal hydrolase (UCH) and that ubiquitination and degradation of phospho-IκB-α were enhanced by vsp21 via competitive binding to UCH. Finally, we validated that vsp21 activates the NF-κB/autophagy pathway to suppress viral replication by interacting with UCH. These findings provided new insights into regulating viral multiplication and reovirus-host interaction. [Display omitted] •BmCPV was demonstrated to generate a viral circRNA, vcircRNA_000048, which encodes a vsp21 to suppress viral replication.•vsp21 induces ROS generation, activates autophagy, and attenuates virus replication by inducing autophagy.•vsp21 activates the NF-κB/autophagy pathway to suppress viral replication by interacting with UCH.</description><identifier>ISSN: 0965-1748</identifier><identifier>EISSN: 1879-0240</identifier><identifier>DOI: 10.1016/j.ibmb.2023.103947</identifier><identifier>PMID: 37086910</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Autophagy ; BmCPV ; Bombyx - genetics ; Bombyx - metabolism ; Circular RNA ; Hydrolases - metabolism ; NF-kappa B - metabolism ; NF-κB ; Reoviridae - genetics ; Reoviridae - metabolism ; Ubiquitins - metabolism ; Virus Replication ; vsp21</subject><ispartof>Insect biochemistry and molecular biology, 2023-05, Vol.156, p.103947-103947, Article 103947</ispartof><rights>2023</rights><rights>Copyright © 2023. 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subjects Animals
Autophagy
BmCPV
Bombyx - genetics
Bombyx - metabolism
Circular RNA
Hydrolases - metabolism
NF-kappa B - metabolism
NF-κB
Reoviridae - genetics
Reoviridae - metabolism
Ubiquitins - metabolism
Virus Replication
vsp21
title BmCPV replication is suppressed by the activation of the NF-κB/autophagy pathway through the interaction of vsp21 translated by vcircRNA_000048 with ubiquitin carboxyl-terminal hydrolase
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