UHPLC‐Orbitrap‐Fusion‐TMS‐Based Metabolomics Study of Phenylpropionamides in the Seed of Cannabis sativa L. against Alzheimer's Disease
Phenylpropionamides in the seed of Cannabis sativa L. (PHS) have a protective effect on neuroinflammation and antioxidant activity. In this study, the UHPLC‐Orbitrap‐fusion‐TMS‐based metabolomics approach was used to analyze the serum samples and identify potential biomarkers in Streptozotocin (STZ)...
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| Veröffentlicht in: | Chemistry & biodiversity 2023-05, Vol.20 (5), p.e202201047-n/a |
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| creator | Wu, Dan‐Dan Liu, Yan Guan, Wei Pan, Juan Kuang, Hai‐Xue Yang, Bing‐You |
| description | Phenylpropionamides in the seed of Cannabis sativa L. (PHS) have a protective effect on neuroinflammation and antioxidant activity. In this study, the UHPLC‐Orbitrap‐fusion‐TMS‐based metabolomics approach was used to analyze the serum samples and identify potential biomarkers in Streptozotocin (STZ) induced Alzheimer's disease (AD) rats. The results revealed that primary bile acid biosynthesis and taurine and hypotaurine metabolism were significantly correlated with STZ‐induced AD rats. In addition, the key enzymes in these two pathways were verified at the protein level. The levels of cysteine dioxygenase type I (CDO1), cysteine sulfinic acid decarboxylase (CSAD), cysteamine (2‐aminoethanethiol) dioxygenase (ADO), 7α‐hydroxylase (CYP7A1), and sterol 12α‐hydroxylase (CYP8B1) were the key enzymes affecting the two pathways in AD rats compared with the control group (CON). Furthermore, after a high‐dose group of phenylpropionamides in the seed of Cannabis sativa L. (PHS−H) was administrated, the levels of CDO1, CSAD, CYP7A1, and CYP8B1 were all callback. These findings demonstrate for the first time that the anti‐AD effect of PHS is associated with the regulation of primary bile acid biosynthesis and taurine and hypotaurine metabolism in STZ‐induced AD rats. |
| doi_str_mv | 10.1002/cbdv.202201047 |
| format | Article |
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(PHS) have a protective effect on neuroinflammation and antioxidant activity. In this study, the UHPLC‐Orbitrap‐fusion‐TMS‐based metabolomics approach was used to analyze the serum samples and identify potential biomarkers in Streptozotocin (STZ) induced Alzheimer's disease (AD) rats. The results revealed that primary bile acid biosynthesis and taurine and hypotaurine metabolism were significantly correlated with STZ‐induced AD rats. In addition, the key enzymes in these two pathways were verified at the protein level. The levels of cysteine dioxygenase type I (CDO1), cysteine sulfinic acid decarboxylase (CSAD), cysteamine (2‐aminoethanethiol) dioxygenase (ADO), 7α‐hydroxylase (CYP7A1), and sterol 12α‐hydroxylase (CYP8B1) were the key enzymes affecting the two pathways in AD rats compared with the control group (CON). Furthermore, after a high‐dose group of phenylpropionamides in the seed of Cannabis sativa L. (PHS−H) was administrated, the levels of CDO1, CSAD, CYP7A1, and CYP8B1 were all callback. These findings demonstrate for the first time that the anti‐AD effect of PHS is associated with the regulation of primary bile acid biosynthesis and taurine and hypotaurine metabolism in STZ‐induced AD rats.</description><identifier>ISSN: 1612-1872</identifier><identifier>EISSN: 1612-1880</identifier><identifier>DOI: 10.1002/cbdv.202201047</identifier><identifier>PMID: 37072341</identifier><language>eng</language><publisher>Switzerland: Wiley Subscription Services, Inc</publisher><subject>Alzheimer Disease - chemically induced ; Alzheimer Disease - drug therapy ; Alzheimer's disease ; Animals ; Bile Acids and Salts ; Biomarkers ; Biosynthesis ; Cannabis ; Cannabis sativa ; Cannabis sativa L ; Chromatography, High Pressure Liquid ; Cysteamine ; Cysteine ; Cysteine dioxygenase ; Enzymes ; Inflammation ; Marijuana ; Metabolism ; Metabolomics ; Neurodegenerative diseases ; phenylpropionamides ; Rats ; Steroid 12-alpha-Hydroxylase ; Streptozocin ; Taurine ; Taurine - metabolism ; Taurine - pharmacology</subject><ispartof>Chemistry & biodiversity, 2023-05, Vol.20 (5), p.e202201047-n/a</ispartof><rights>2023 Wiley‐VHCA AG, Zurich, Switzerland</rights><rights>2023 Wiley-VHCA AG, Zurich, Switzerland.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3737-e1936cc380b2b6c9b87d2346b1a10443880d00de6b7570fd1a30e5cd27e3cf923</citedby><cites>FETCH-LOGICAL-c3737-e1936cc380b2b6c9b87d2346b1a10443880d00de6b7570fd1a30e5cd27e3cf923</cites><orcidid>0000-0002-2310-2750</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbdv.202201047$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbdv.202201047$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37072341$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Dan‐Dan</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Guan, Wei</creatorcontrib><creatorcontrib>Pan, Juan</creatorcontrib><creatorcontrib>Kuang, Hai‐Xue</creatorcontrib><creatorcontrib>Yang, Bing‐You</creatorcontrib><title>UHPLC‐Orbitrap‐Fusion‐TMS‐Based Metabolomics Study of Phenylpropionamides in the Seed of Cannabis sativa L. against Alzheimer's Disease</title><title>Chemistry & biodiversity</title><addtitle>Chem Biodivers</addtitle><description>Phenylpropionamides in the seed of Cannabis sativa L. (PHS) have a protective effect on neuroinflammation and antioxidant activity. In this study, the UHPLC‐Orbitrap‐fusion‐TMS‐based metabolomics approach was used to analyze the serum samples and identify potential biomarkers in Streptozotocin (STZ) induced Alzheimer's disease (AD) rats. The results revealed that primary bile acid biosynthesis and taurine and hypotaurine metabolism were significantly correlated with STZ‐induced AD rats. In addition, the key enzymes in these two pathways were verified at the protein level. The levels of cysteine dioxygenase type I (CDO1), cysteine sulfinic acid decarboxylase (CSAD), cysteamine (2‐aminoethanethiol) dioxygenase (ADO), 7α‐hydroxylase (CYP7A1), and sterol 12α‐hydroxylase (CYP8B1) were the key enzymes affecting the two pathways in AD rats compared with the control group (CON). Furthermore, after a high‐dose group of phenylpropionamides in the seed of Cannabis sativa L. (PHS−H) was administrated, the levels of CDO1, CSAD, CYP7A1, and CYP8B1 were all callback. These findings demonstrate for the first time that the anti‐AD effect of PHS is associated with the regulation of primary bile acid biosynthesis and taurine and hypotaurine metabolism in STZ‐induced AD rats.</description><subject>Alzheimer Disease - chemically induced</subject><subject>Alzheimer Disease - drug therapy</subject><subject>Alzheimer's disease</subject><subject>Animals</subject><subject>Bile Acids and Salts</subject><subject>Biomarkers</subject><subject>Biosynthesis</subject><subject>Cannabis</subject><subject>Cannabis sativa</subject><subject>Cannabis sativa L</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Cysteamine</subject><subject>Cysteine</subject><subject>Cysteine dioxygenase</subject><subject>Enzymes</subject><subject>Inflammation</subject><subject>Marijuana</subject><subject>Metabolism</subject><subject>Metabolomics</subject><subject>Neurodegenerative diseases</subject><subject>phenylpropionamides</subject><subject>Rats</subject><subject>Steroid 12-alpha-Hydroxylase</subject><subject>Streptozocin</subject><subject>Taurine</subject><subject>Taurine - metabolism</subject><subject>Taurine - pharmacology</subject><issn>1612-1872</issn><issn>1612-1880</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vEzEQhi1ERdvAlSOyxIFeEvyRrjfHdktbpFStlJbryh-zxNV-BM9uUTjxD-A39pcwUdogceFij6znfT0zL2NvpZhIIdRH78LDRAmlhBRT84IdyEyqscxz8XJXG7XPDhHviaf3_BXb10YYpafygP26u7yZF48_f18nF_tkV1SeDxi7lorbqwWdpxYh8Cvorevqroke-aIfwpp3Fb9ZQruuV6lbkcI2MQDy2PJ-CXwBpCKksG1rXUSOto8Pls8n3H61scWen9Q_lhAbSB-Qn0UE-ug126tsjfDm6R6xu_NPt8XleH598bk4mY-9NtqMQc505r3OhVMu8zOXm0ADZU5a2sNU05xBiACZM8dGVEFaLeDYB2VA-2qm9IgdbX2p928DYF82ET3UtW2hG7BUudBai43XiL3_B73vhtRSd0RJ2qOampyoyZbyqUNMUJWrFBub1qUU5SaqchNVuYuKBO-ebAfXQNjhz9kQMNsC32MN6__YlcXp2Ze_5n8AdDWjmg</recordid><startdate>202305</startdate><enddate>202305</enddate><creator>Wu, Dan‐Dan</creator><creator>Liu, Yan</creator><creator>Guan, Wei</creator><creator>Pan, Juan</creator><creator>Kuang, Hai‐Xue</creator><creator>Yang, Bing‐You</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2310-2750</orcidid></search><sort><creationdate>202305</creationdate><title>UHPLC‐Orbitrap‐Fusion‐TMS‐Based Metabolomics Study of Phenylpropionamides in the Seed of Cannabis sativa L. against Alzheimer's Disease</title><author>Wu, Dan‐Dan ; Liu, Yan ; Guan, Wei ; Pan, Juan ; Kuang, Hai‐Xue ; Yang, Bing‐You</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3737-e1936cc380b2b6c9b87d2346b1a10443880d00de6b7570fd1a30e5cd27e3cf923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Alzheimer Disease - chemically induced</topic><topic>Alzheimer Disease - drug therapy</topic><topic>Alzheimer's disease</topic><topic>Animals</topic><topic>Bile Acids and Salts</topic><topic>Biomarkers</topic><topic>Biosynthesis</topic><topic>Cannabis</topic><topic>Cannabis sativa</topic><topic>Cannabis sativa L</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Cysteamine</topic><topic>Cysteine</topic><topic>Cysteine dioxygenase</topic><topic>Enzymes</topic><topic>Inflammation</topic><topic>Marijuana</topic><topic>Metabolism</topic><topic>Metabolomics</topic><topic>Neurodegenerative diseases</topic><topic>phenylpropionamides</topic><topic>Rats</topic><topic>Steroid 12-alpha-Hydroxylase</topic><topic>Streptozocin</topic><topic>Taurine</topic><topic>Taurine - metabolism</topic><topic>Taurine - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Dan‐Dan</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Guan, Wei</creatorcontrib><creatorcontrib>Pan, Juan</creatorcontrib><creatorcontrib>Kuang, Hai‐Xue</creatorcontrib><creatorcontrib>Yang, Bing‐You</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Chemistry & biodiversity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Dan‐Dan</au><au>Liu, Yan</au><au>Guan, Wei</au><au>Pan, Juan</au><au>Kuang, Hai‐Xue</au><au>Yang, Bing‐You</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>UHPLC‐Orbitrap‐Fusion‐TMS‐Based Metabolomics Study of Phenylpropionamides in the Seed of Cannabis sativa L. against Alzheimer's Disease</atitle><jtitle>Chemistry & biodiversity</jtitle><addtitle>Chem Biodivers</addtitle><date>2023-05</date><risdate>2023</risdate><volume>20</volume><issue>5</issue><spage>e202201047</spage><epage>n/a</epage><pages>e202201047-n/a</pages><issn>1612-1872</issn><eissn>1612-1880</eissn><abstract>Phenylpropionamides in the seed of Cannabis sativa L. (PHS) have a protective effect on neuroinflammation and antioxidant activity. In this study, the UHPLC‐Orbitrap‐fusion‐TMS‐based metabolomics approach was used to analyze the serum samples and identify potential biomarkers in Streptozotocin (STZ) induced Alzheimer's disease (AD) rats. The results revealed that primary bile acid biosynthesis and taurine and hypotaurine metabolism were significantly correlated with STZ‐induced AD rats. In addition, the key enzymes in these two pathways were verified at the protein level. The levels of cysteine dioxygenase type I (CDO1), cysteine sulfinic acid decarboxylase (CSAD), cysteamine (2‐aminoethanethiol) dioxygenase (ADO), 7α‐hydroxylase (CYP7A1), and sterol 12α‐hydroxylase (CYP8B1) were the key enzymes affecting the two pathways in AD rats compared with the control group (CON). Furthermore, after a high‐dose group of phenylpropionamides in the seed of Cannabis sativa L. (PHS−H) was administrated, the levels of CDO1, CSAD, CYP7A1, and CYP8B1 were all callback. These findings demonstrate for the first time that the anti‐AD effect of PHS is associated with the regulation of primary bile acid biosynthesis and taurine and hypotaurine metabolism in STZ‐induced AD rats.</abstract><cop>Switzerland</cop><pub>Wiley Subscription Services, Inc</pub><pmid>37072341</pmid><doi>10.1002/cbdv.202201047</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-2310-2750</orcidid></addata></record> |
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| ispartof | Chemistry & biodiversity, 2023-05, Vol.20 (5), p.e202201047-n/a |
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| subjects | Alzheimer Disease - chemically induced Alzheimer Disease - drug therapy Alzheimer's disease Animals Bile Acids and Salts Biomarkers Biosynthesis Cannabis Cannabis sativa Cannabis sativa L Chromatography, High Pressure Liquid Cysteamine Cysteine Cysteine dioxygenase Enzymes Inflammation Marijuana Metabolism Metabolomics Neurodegenerative diseases phenylpropionamides Rats Steroid 12-alpha-Hydroxylase Streptozocin Taurine Taurine - metabolism Taurine - pharmacology |
| title | UHPLC‐Orbitrap‐Fusion‐TMS‐Based Metabolomics Study of Phenylpropionamides in the Seed of Cannabis sativa L. against Alzheimer's Disease |
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