An efficient Agrobacterium-mediated system based on the pyrG auxotrophic marker for recombinant expression in the filamentous fungus Penicillium rubens

Objectives This work aimed to construct a versatile, effective, and food-grade Agrobacterium tumefaciens -mediated transformation (ATMT) system for recombinant expression in the filamentous fungus Penicillium rubens (also known as Pencillium chrysogenum ). Results In this study, the wild-type P. chr...

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Veröffentlicht in:	Biotechnology letters 2023-06, Vol.45 (5-6), p.689-702
Hauptverfasser:	Tran, Van-Tuan, Thai, Hanh-Dung, Vu, Tao Xuan, Vu, Ha Hong, Nguyen, Giang Thu, Trinh, Minh Thi, Tran, Huyen Thi Thanh, Pham, Huong Thi Thu, Le, Nhung Thi Hong
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrobacterium tumefaciens - genetics Antibiotics Applied Microbiology Biochemistry Biomedical and Life Sciences Biosynthesis Biotechnology Drug resistance Enzymes Fluorescence microscopy Fungi Genes Genetic engineering Genetic transformation Genomes Homologous recombination Life Sciences Metabolites Microbiology Original Research Paper Penicillin Penicillium - genetics Penicillium - metabolism Penicillium rubens PHYA gene Phytase PyrG gene Reporter gene RNA polymerase Sequence analysis Spores Transformation, Genetic Uracil Uracil - metabolism Uridine
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