Ultra-sensitive detection of protein thermal unfolding and refolding using near-zone microwaves
We use planar slot antennas proximal to proteins in solution to detect changes in conformation. The antennas are attached to fused-quartz or glass sample holders and the cuvette/antenna assembly is placed in the sample holder of an optical spectrophotomer (either UV/VIS or fluorescence polarization)...
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| Veröffentlicht in: | IEEE transactions on microwave theory and techniques 2005-05, Vol.53 (5), p.1576-1586 |
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| container_end_page | 1586 |
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| container_issue | 5 |
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| container_title | IEEE transactions on microwave theory and techniques |
| container_volume | 53 |
| creator | Taylor, K.M. van der Weide, D.W. |
| description | We use planar slot antennas proximal to proteins in solution to detect changes in conformation. The antennas are attached to fused-quartz or glass sample holders and the cuvette/antenna assembly is placed in the sample holder of an optical spectrophotomer (either UV/VIS or fluorescence polarization), allowing simultaneous dielectric and optical measurements. Return loss is recorded using a vector network analyzer. This system was used to study the equilibrium thermal unfolding and refolding of a small globular protein, as well as the binding of small hormones to a receptor. Good agreement between optical and microwave measurements was obtained for all systems studied. We show that microwave measurements can be made at protein concentrations as low as 0.3 ng/mL (19 pM), several orders of magnitude lower than that required for optical spectroscopy. The results from these experiments demonstrate that resonant slot antennas can be used to detect changes in protein conformation and the presence of microwave radiation does not perturb the system under study. |
| doi_str_mv | 10.1109/TMTT.2005.847047 |
| format | Article |
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The antennas are attached to fused-quartz or glass sample holders and the cuvette/antenna assembly is placed in the sample holder of an optical spectrophotomer (either UV/VIS or fluorescence polarization), allowing simultaneous dielectric and optical measurements. Return loss is recorded using a vector network analyzer. This system was used to study the equilibrium thermal unfolding and refolding of a small globular protein, as well as the binding of small hormones to a receptor. Good agreement between optical and microwave measurements was obtained for all systems studied. We show that microwave measurements can be made at protein concentrations as low as 0.3 ng/mL (19 pM), several orders of magnitude lower than that required for optical spectroscopy. 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The antennas are attached to fused-quartz or glass sample holders and the cuvette/antenna assembly is placed in the sample holder of an optical spectrophotomer (either UV/VIS or fluorescence polarization), allowing simultaneous dielectric and optical measurements. Return loss is recorded using a vector network analyzer. This system was used to study the equilibrium thermal unfolding and refolding of a small globular protein, as well as the binding of small hormones to a receptor. Good agreement between optical and microwave measurements was obtained for all systems studied. We show that microwave measurements can be made at protein concentrations as low as 0.3 ng/mL (19 pM), several orders of magnitude lower than that required for optical spectroscopy. The results from these experiments demonstrate that resonant slot antennas can be used to detect changes in protein conformation and the presence of microwave radiation does not perturb the system under study.</description><subject>Antenna measurements</subject><subject>Antennas</subject><subject>Assembly</subject><subject>Binding</subject><subject>Biological and medical sciences</subject><subject>Biomedical applications of electromagnetic radiation</subject><subject>biophysics</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Dielectric measurements</subject><subject>Fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glass</subject><subject>Methods. Procedures. Technologies</subject><subject>Microwave measurements</subject><subject>Microwaves</subject><subject>Networks</subject><subject>Optical polarization</subject><subject>Optical recording</subject><subject>Proteins</subject><subject>Sample holders</subject><subject>Slot antennas</subject><subject>Various methods and equipments</subject><issn>0018-9480</issn><issn>1557-9670</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>RIE</sourceid><recordid>eNp9kUFrGzEQhUVoIa7beyGXJZD0tM5Iq5W0x2CapuCQi30WsnbUKqy1ibTrkv76arGDIYdeZhj0zUNvHiFfKSwoheZm_bBeLxhAvVBcApdnZEbrWpaNkPCBzACoKhuu4Jx8Sukpj7wGNSN60w3RlAlD8oPfY9HigHbwfSh6VzzHfkAfiuE3xp3pijG4vmt9-FWY0BYR36YxTTWgieXfPmCx8zb2f8we02fy0Zku4Zdjn5PN3ff18r5cPf74ubxdlZY3dCgpCstt1VLTOEfbLSoG1AlozBZNLU2F2YtDJ1UlUYCwbAsWalZJJy1AVc3Jt4Nu_vLLiGnQO58sdp0J2I9Jq0Ywprjimbz-L8kUsHyzCbx8Bz71YwzZhVZCcsE5rzMEBygbTilfRD9HvzPxVVPQUzB6CkZPwehDMHnl6qhrkjWdiyZYn057QkoqYZK-OHAeEU_PnMlGQPUPru6XqA</recordid><startdate>20050501</startdate><enddate>20050501</enddate><creator>Taylor, K.M.</creator><creator>van der Weide, D.W.</creator><general>IEEE</general><general>Institute of Electrical and Electronics Engineers</general><general>The Institute of Electrical and Electronics Engineers, Inc. (IEEE)</general><scope>97E</scope><scope>RIA</scope><scope>RIE</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>8FD</scope><scope>L7M</scope><scope>F28</scope><scope>FR3</scope></search><sort><creationdate>20050501</creationdate><title>Ultra-sensitive detection of protein thermal unfolding and refolding using near-zone microwaves</title><author>Taylor, K.M. ; van der Weide, D.W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-1e6c4c3d1a9ff1dbe8201f609abea57a3e557fef7837e606c2b0c05237f7c0033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Antenna measurements</topic><topic>Antennas</topic><topic>Assembly</topic><topic>Binding</topic><topic>Biological and medical sciences</topic><topic>Biomedical applications of electromagnetic radiation</topic><topic>biophysics</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Dielectric measurements</topic><topic>Fluorescence</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glass</topic><topic>Methods. Procedures. Technologies</topic><topic>Microwave measurements</topic><topic>Microwaves</topic><topic>Networks</topic><topic>Optical polarization</topic><topic>Optical recording</topic><topic>Proteins</topic><topic>Sample holders</topic><topic>Slot antennas</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Taylor, K.M.</creatorcontrib><creatorcontrib>van der Weide, D.W.</creatorcontrib><collection>IEEE All-Society Periodicals Package (ASPP) 2005-present</collection><collection>IEEE All-Society Periodicals Package (ASPP) 1998-Present</collection><collection>IEEE Electronic Library (IEL)</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><jtitle>IEEE transactions on microwave theory and techniques</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Taylor, K.M.</au><au>van der Weide, D.W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultra-sensitive detection of protein thermal unfolding and refolding using near-zone microwaves</atitle><jtitle>IEEE transactions on microwave theory and techniques</jtitle><stitle>TMTT</stitle><date>2005-05-01</date><risdate>2005</risdate><volume>53</volume><issue>5</issue><spage>1576</spage><epage>1586</epage><pages>1576-1586</pages><issn>0018-9480</issn><eissn>1557-9670</eissn><coden>IETMAB</coden><abstract>We use planar slot antennas proximal to proteins in solution to detect changes in conformation. The antennas are attached to fused-quartz or glass sample holders and the cuvette/antenna assembly is placed in the sample holder of an optical spectrophotomer (either UV/VIS or fluorescence polarization), allowing simultaneous dielectric and optical measurements. Return loss is recorded using a vector network analyzer. This system was used to study the equilibrium thermal unfolding and refolding of a small globular protein, as well as the binding of small hormones to a receptor. Good agreement between optical and microwave measurements was obtained for all systems studied. We show that microwave measurements can be made at protein concentrations as low as 0.3 ng/mL (19 pM), several orders of magnitude lower than that required for optical spectroscopy. The results from these experiments demonstrate that resonant slot antennas can be used to detect changes in protein conformation and the presence of microwave radiation does not perturb the system under study.</abstract><cop>New York, NY</cop><pub>IEEE</pub><doi>10.1109/TMTT.2005.847047</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0018-9480 |
| ispartof | IEEE transactions on microwave theory and techniques, 2005-05, Vol.53 (5), p.1576-1586 |
| issn | 0018-9480 1557-9670 |
| language | eng |
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| source | IEEE Electronic Library (IEL) |
| subjects | Antenna measurements Antennas Assembly Binding Biological and medical sciences Biomedical applications of electromagnetic radiation biophysics Biosensors Biotechnology Dielectric measurements Fluorescence Fundamental and applied biological sciences. Psychology Glass Methods. Procedures. Technologies Microwave measurements Microwaves Networks Optical polarization Optical recording Proteins Sample holders Slot antennas Various methods and equipments |
| title | Ultra-sensitive detection of protein thermal unfolding and refolding using near-zone microwaves |
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