Physical and chemical characterization of enolase immobilized polydiacetylene Langmuir–Blodgett film

Hexa histidine tagged recombinant Plasmodium falciparum enolase (His6-Pfen) was covalently immobilized on a Langmuir–Blodgett film of a self assembled mixture of 10,12-pentacosadiynoic acid and its N-succinimidyl ester derivative (PDA LB-film). The film was polymerized with a UV-lamp at 254nm to obt...

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Veröffentlicht in:	Sensors and actuators. B, Chemical Chemical, 2006-05, Vol.115 (1), p.526-533
Hauptverfasser:	Sadagopan, K., Sawant, Shilpa N., Kulshreshtha, S.K., Jarori, Gotam K.
Format:	Artikel
Sprache:	eng
Schlagworte:	AFM Colorimetric response Covalent immobilization Enolase Plasmodium falciparum Protein–ligand interaction
Online-Zugang:	Volltext
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