Antibacterial and fluorescent clear aligner attachment resin modified with chlorhexidine loaded mesoporous silica nanoparticles and zinc oxide quantum dots

To develop an antibacterial and fluorescent clear aligner attachment resin via the incorporation of chlorhexidine loaded pore-expanded mesoporous silica nanoparticles (CHX@pMSN) and amino-silane functionalized zinc oxide quantum dots (aZnOQDs), and to evaluate its antibacterial activity, fluorescenc...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of the mechanical behavior of biomedical materials 2023-05, Vol.141, p.105817-105817, Article 105817
Hauptverfasser: Cao, Lingyun, Yan, Jiarong, Luo, Ting, Yan, Huiyi, Hua, Fang, He, Hong
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 105817
container_issue
container_start_page 105817
container_title Journal of the mechanical behavior of biomedical materials
container_volume 141
creator Cao, Lingyun
Yan, Jiarong
Luo, Ting
Yan, Huiyi
Hua, Fang
He, Hong
description To develop an antibacterial and fluorescent clear aligner attachment resin via the incorporation of chlorhexidine loaded pore-expanded mesoporous silica nanoparticles (CHX@pMSN) and amino-silane functionalized zinc oxide quantum dots (aZnOQDs), and to evaluate its antibacterial activity, fluorescence capability, esthetic properties, mechanical performance and biocompatibility. CHX@pMSN and aZnOQDs were incorporated into the commercial resin composites (Filtek Z350 XT, 3M) at different mass fractions, control group: Filtek; fluorescent attachment resin (FAR): Filtek + 3 wt% aZnOQDs; antibacterial and fluorescent attachment resin (AFAR)-1: Filtek + 3 wt% aZnOQDs + 1 wt% CHX@pMSN; AFAR-2: Filtek + 3 wt% aZnOQDs + 3 wt% CHX@pMSN; AFAR-3: Filtek + 3 wt% aZnOQDs + 5 wt% CHX@pMSN. CHX release, antibacterial activity, fluorescence capability, color change, stain resistance, degree of conversion, depth of cure, polymerization shrinkage, water sorption and solubility, softening in solvent, flexural strength, flexural modulus, shear bond strength, and cytotoxicity were evaluated comprehensively. CHX could be continuously released from the AFAR groups for up to 30 days. CFU, MTT, lactic acid production, SEM and CLSM evaluation showed AFAR-2 and AFAR-3 could effectively inhibit S. mutans biofilms even after 1-month aging. Only AFAR-3 showed clinically perceptible color change and all the experimental groups were not more susceptible to staining. AFAR-1 and AFAR-2 could suppress polymerization shrinkage and enhance the resistance to degradation without compromising other properties, including degree of conversion, water sorption and solubility, flexural strength, flexural modulus, and shear bond strength. Depth of cure of all the four experimental groups was significantly decreased (p 
doi_str_mv 10.1016/j.jmbbm.2023.105817
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2796162331</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1751616123001704</els_id><sourcerecordid>2796162331</sourcerecordid><originalsourceid>FETCH-LOGICAL-c404t-ace229d88e22d30a63d29f4954657b7d76a0bf9e554cabf9455c728a65d705ee3</originalsourceid><addsrcrecordid>eNp9UU1v1DAQjRAV_YBfUKnykUsWO4nj5MChqsqHVIlLOVsTe8LOyrG3tgOFv8KfxdstHDm9kee9eTN-VXUp-EZw0b_bbXbLNC2bhjdteZGDUC-qMzGooeZi4C9LraSoe9GL0-o8pR3nPefD8Ko6bRUXUnTqrPp97TNNYDJGAsfAWza7NURMBn1mxiFEBo6-eSyYM5jtcmgUAnm2BEszoWU_KG-Z2boQt_hIljwyF8CWzoIp7EMMa2KJHBlgHnzYQ8xUhqcnx1_kDQtFh-xhBZ_XhdmQ0-vqZAaX8M0zXlRfP9ze33yq7758_HxzfVebjne5BoNNM9phKGBbDn1rm3HuRtn1Uk3Kqh74NI8oZWegFJ2URjUD9NIqLhHbi-rtce4-hocVU9YLlfOdA49lb92osXxi07aiUNsj1cSQUsRZ7yMtEH9qwfUhFb3TT6noQyr6mEpRXT0brNOC9p_mbwyF8P5IwHLmd8KokyH0Bi1FNFnbQP81-AO_56M7</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2796162331</pqid></control><display><type>article</type><title>Antibacterial and fluorescent clear aligner attachment resin modified with chlorhexidine loaded mesoporous silica nanoparticles and zinc oxide quantum dots</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Cao, Lingyun ; Yan, Jiarong ; Luo, Ting ; Yan, Huiyi ; Hua, Fang ; He, Hong</creator><creatorcontrib>Cao, Lingyun ; Yan, Jiarong ; Luo, Ting ; Yan, Huiyi ; Hua, Fang ; He, Hong</creatorcontrib><description>To develop an antibacterial and fluorescent clear aligner attachment resin via the incorporation of chlorhexidine loaded pore-expanded mesoporous silica nanoparticles (CHX@pMSN) and amino-silane functionalized zinc oxide quantum dots (aZnOQDs), and to evaluate its antibacterial activity, fluorescence capability, esthetic properties, mechanical performance and biocompatibility. CHX@pMSN and aZnOQDs were incorporated into the commercial resin composites (Filtek Z350 XT, 3M) at different mass fractions, control group: Filtek; fluorescent attachment resin (FAR): Filtek + 3 wt% aZnOQDs; antibacterial and fluorescent attachment resin (AFAR)-1: Filtek + 3 wt% aZnOQDs + 1 wt% CHX@pMSN; AFAR-2: Filtek + 3 wt% aZnOQDs + 3 wt% CHX@pMSN; AFAR-3: Filtek + 3 wt% aZnOQDs + 5 wt% CHX@pMSN. CHX release, antibacterial activity, fluorescence capability, color change, stain resistance, degree of conversion, depth of cure, polymerization shrinkage, water sorption and solubility, softening in solvent, flexural strength, flexural modulus, shear bond strength, and cytotoxicity were evaluated comprehensively. CHX could be continuously released from the AFAR groups for up to 30 days. CFU, MTT, lactic acid production, SEM and CLSM evaluation showed AFAR-2 and AFAR-3 could effectively inhibit S. mutans biofilms even after 1-month aging. Only AFAR-3 showed clinically perceptible color change and all the experimental groups were not more susceptible to staining. AFAR-1 and AFAR-2 could suppress polymerization shrinkage and enhance the resistance to degradation without compromising other properties, including degree of conversion, water sorption and solubility, flexural strength, flexural modulus, and shear bond strength. Depth of cure of all the four experimental groups was significantly decreased (p &lt; 0.05) but still within the ISO standard. CCK-8 assay and live/dead cell staining denied the cytotoxicity of experimental resins. Fluorescence intensity tests showed that FAR and AFAR-2 could emit strong yellowish fluorescence under the excitation of ultraviolet for up to six months. AFRA-2 possessed long-term antibiofilm activity, strong fluorescence capability and satisfying biocompatibility without compromising esthetic and mechanical properties. This study proposed a new strategy for reducing bacteria accumulation around the attachment, which is also promising in helping orthodontists to remove the attachment thoroughly and precisely.</description><identifier>ISSN: 1751-6161</identifier><identifier>EISSN: 1878-0180</identifier><identifier>DOI: 10.1016/j.jmbbm.2023.105817</identifier><identifier>PMID: 37015147</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - pharmacology ; Antibacterial activity ; Chlorhexidine ; Chlorhexidine - chemistry ; Chlorhexidine - pharmacology ; Clear aligner attachment ; Composite Resins - chemistry ; Fluorescence ; Materials Testing ; Mesoporous silica nanoparticles ; Methacrylates - chemistry ; Nanoparticles - chemistry ; Orthodontic Appliances, Removable ; Quantum Dots ; Silicon Dioxide - chemistry ; Water ; Zinc Oxide ; Zinc oxide quantum dots</subject><ispartof>Journal of the mechanical behavior of biomedical materials, 2023-05, Vol.141, p.105817-105817, Article 105817</ispartof><rights>2023 Elsevier Ltd</rights><rights>Copyright © 2023 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-ace229d88e22d30a63d29f4954657b7d76a0bf9e554cabf9455c728a65d705ee3</citedby><cites>FETCH-LOGICAL-c404t-ace229d88e22d30a63d29f4954657b7d76a0bf9e554cabf9455c728a65d705ee3</cites><orcidid>0000-0002-9234-0015 ; 0000-0002-2438-5924 ; 0000-0001-6409-6708 ; 0000-0002-9733-6402</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1751616123001704$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37015147$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cao, Lingyun</creatorcontrib><creatorcontrib>Yan, Jiarong</creatorcontrib><creatorcontrib>Luo, Ting</creatorcontrib><creatorcontrib>Yan, Huiyi</creatorcontrib><creatorcontrib>Hua, Fang</creatorcontrib><creatorcontrib>He, Hong</creatorcontrib><title>Antibacterial and fluorescent clear aligner attachment resin modified with chlorhexidine loaded mesoporous silica nanoparticles and zinc oxide quantum dots</title><title>Journal of the mechanical behavior of biomedical materials</title><addtitle>J Mech Behav Biomed Mater</addtitle><description>To develop an antibacterial and fluorescent clear aligner attachment resin via the incorporation of chlorhexidine loaded pore-expanded mesoporous silica nanoparticles (CHX@pMSN) and amino-silane functionalized zinc oxide quantum dots (aZnOQDs), and to evaluate its antibacterial activity, fluorescence capability, esthetic properties, mechanical performance and biocompatibility. CHX@pMSN and aZnOQDs were incorporated into the commercial resin composites (Filtek Z350 XT, 3M) at different mass fractions, control group: Filtek; fluorescent attachment resin (FAR): Filtek + 3 wt% aZnOQDs; antibacterial and fluorescent attachment resin (AFAR)-1: Filtek + 3 wt% aZnOQDs + 1 wt% CHX@pMSN; AFAR-2: Filtek + 3 wt% aZnOQDs + 3 wt% CHX@pMSN; AFAR-3: Filtek + 3 wt% aZnOQDs + 5 wt% CHX@pMSN. CHX release, antibacterial activity, fluorescence capability, color change, stain resistance, degree of conversion, depth of cure, polymerization shrinkage, water sorption and solubility, softening in solvent, flexural strength, flexural modulus, shear bond strength, and cytotoxicity were evaluated comprehensively. CHX could be continuously released from the AFAR groups for up to 30 days. CFU, MTT, lactic acid production, SEM and CLSM evaluation showed AFAR-2 and AFAR-3 could effectively inhibit S. mutans biofilms even after 1-month aging. Only AFAR-3 showed clinically perceptible color change and all the experimental groups were not more susceptible to staining. AFAR-1 and AFAR-2 could suppress polymerization shrinkage and enhance the resistance to degradation without compromising other properties, including degree of conversion, water sorption and solubility, flexural strength, flexural modulus, and shear bond strength. Depth of cure of all the four experimental groups was significantly decreased (p &lt; 0.05) but still within the ISO standard. CCK-8 assay and live/dead cell staining denied the cytotoxicity of experimental resins. Fluorescence intensity tests showed that FAR and AFAR-2 could emit strong yellowish fluorescence under the excitation of ultraviolet for up to six months. AFRA-2 possessed long-term antibiofilm activity, strong fluorescence capability and satisfying biocompatibility without compromising esthetic and mechanical properties. This study proposed a new strategy for reducing bacteria accumulation around the attachment, which is also promising in helping orthodontists to remove the attachment thoroughly and precisely.</description><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibacterial activity</subject><subject>Chlorhexidine</subject><subject>Chlorhexidine - chemistry</subject><subject>Chlorhexidine - pharmacology</subject><subject>Clear aligner attachment</subject><subject>Composite Resins - chemistry</subject><subject>Fluorescence</subject><subject>Materials Testing</subject><subject>Mesoporous silica nanoparticles</subject><subject>Methacrylates - chemistry</subject><subject>Nanoparticles - chemistry</subject><subject>Orthodontic Appliances, Removable</subject><subject>Quantum Dots</subject><subject>Silicon Dioxide - chemistry</subject><subject>Water</subject><subject>Zinc Oxide</subject><subject>Zinc oxide quantum dots</subject><issn>1751-6161</issn><issn>1878-0180</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1v1DAQjRAV_YBfUKnykUsWO4nj5MChqsqHVIlLOVsTe8LOyrG3tgOFv8KfxdstHDm9kee9eTN-VXUp-EZw0b_bbXbLNC2bhjdteZGDUC-qMzGooeZi4C9LraSoe9GL0-o8pR3nPefD8Ko6bRUXUnTqrPp97TNNYDJGAsfAWza7NURMBn1mxiFEBo6-eSyYM5jtcmgUAnm2BEszoWU_KG-Z2boQt_hIljwyF8CWzoIp7EMMa2KJHBlgHnzYQ8xUhqcnx1_kDQtFh-xhBZ_XhdmQ0-vqZAaX8M0zXlRfP9ze33yq7758_HxzfVebjne5BoNNM9phKGBbDn1rm3HuRtn1Uk3Kqh74NI8oZWegFJ2URjUD9NIqLhHbi-rtce4-hocVU9YLlfOdA49lb92osXxi07aiUNsj1cSQUsRZ7yMtEH9qwfUhFb3TT6noQyr6mEpRXT0brNOC9p_mbwyF8P5IwHLmd8KokyH0Bi1FNFnbQP81-AO_56M7</recordid><startdate>202305</startdate><enddate>202305</enddate><creator>Cao, Lingyun</creator><creator>Yan, Jiarong</creator><creator>Luo, Ting</creator><creator>Yan, Huiyi</creator><creator>Hua, Fang</creator><creator>He, Hong</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9234-0015</orcidid><orcidid>https://orcid.org/0000-0002-2438-5924</orcidid><orcidid>https://orcid.org/0000-0001-6409-6708</orcidid><orcidid>https://orcid.org/0000-0002-9733-6402</orcidid></search><sort><creationdate>202305</creationdate><title>Antibacterial and fluorescent clear aligner attachment resin modified with chlorhexidine loaded mesoporous silica nanoparticles and zinc oxide quantum dots</title><author>Cao, Lingyun ; Yan, Jiarong ; Luo, Ting ; Yan, Huiyi ; Hua, Fang ; He, Hong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-ace229d88e22d30a63d29f4954657b7d76a0bf9e554cabf9455c728a65d705ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibacterial activity</topic><topic>Chlorhexidine</topic><topic>Chlorhexidine - chemistry</topic><topic>Chlorhexidine - pharmacology</topic><topic>Clear aligner attachment</topic><topic>Composite Resins - chemistry</topic><topic>Fluorescence</topic><topic>Materials Testing</topic><topic>Mesoporous silica nanoparticles</topic><topic>Methacrylates - chemistry</topic><topic>Nanoparticles - chemistry</topic><topic>Orthodontic Appliances, Removable</topic><topic>Quantum Dots</topic><topic>Silicon Dioxide - chemistry</topic><topic>Water</topic><topic>Zinc Oxide</topic><topic>Zinc oxide quantum dots</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cao, Lingyun</creatorcontrib><creatorcontrib>Yan, Jiarong</creatorcontrib><creatorcontrib>Luo, Ting</creatorcontrib><creatorcontrib>Yan, Huiyi</creatorcontrib><creatorcontrib>Hua, Fang</creatorcontrib><creatorcontrib>He, Hong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the mechanical behavior of biomedical materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cao, Lingyun</au><au>Yan, Jiarong</au><au>Luo, Ting</au><au>Yan, Huiyi</au><au>Hua, Fang</au><au>He, Hong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibacterial and fluorescent clear aligner attachment resin modified with chlorhexidine loaded mesoporous silica nanoparticles and zinc oxide quantum dots</atitle><jtitle>Journal of the mechanical behavior of biomedical materials</jtitle><addtitle>J Mech Behav Biomed Mater</addtitle><date>2023-05</date><risdate>2023</risdate><volume>141</volume><spage>105817</spage><epage>105817</epage><pages>105817-105817</pages><artnum>105817</artnum><issn>1751-6161</issn><eissn>1878-0180</eissn><abstract>To develop an antibacterial and fluorescent clear aligner attachment resin via the incorporation of chlorhexidine loaded pore-expanded mesoporous silica nanoparticles (CHX@pMSN) and amino-silane functionalized zinc oxide quantum dots (aZnOQDs), and to evaluate its antibacterial activity, fluorescence capability, esthetic properties, mechanical performance and biocompatibility. CHX@pMSN and aZnOQDs were incorporated into the commercial resin composites (Filtek Z350 XT, 3M) at different mass fractions, control group: Filtek; fluorescent attachment resin (FAR): Filtek + 3 wt% aZnOQDs; antibacterial and fluorescent attachment resin (AFAR)-1: Filtek + 3 wt% aZnOQDs + 1 wt% CHX@pMSN; AFAR-2: Filtek + 3 wt% aZnOQDs + 3 wt% CHX@pMSN; AFAR-3: Filtek + 3 wt% aZnOQDs + 5 wt% CHX@pMSN. CHX release, antibacterial activity, fluorescence capability, color change, stain resistance, degree of conversion, depth of cure, polymerization shrinkage, water sorption and solubility, softening in solvent, flexural strength, flexural modulus, shear bond strength, and cytotoxicity were evaluated comprehensively. CHX could be continuously released from the AFAR groups for up to 30 days. CFU, MTT, lactic acid production, SEM and CLSM evaluation showed AFAR-2 and AFAR-3 could effectively inhibit S. mutans biofilms even after 1-month aging. Only AFAR-3 showed clinically perceptible color change and all the experimental groups were not more susceptible to staining. AFAR-1 and AFAR-2 could suppress polymerization shrinkage and enhance the resistance to degradation without compromising other properties, including degree of conversion, water sorption and solubility, flexural strength, flexural modulus, and shear bond strength. Depth of cure of all the four experimental groups was significantly decreased (p &lt; 0.05) but still within the ISO standard. CCK-8 assay and live/dead cell staining denied the cytotoxicity of experimental resins. Fluorescence intensity tests showed that FAR and AFAR-2 could emit strong yellowish fluorescence under the excitation of ultraviolet for up to six months. AFRA-2 possessed long-term antibiofilm activity, strong fluorescence capability and satisfying biocompatibility without compromising esthetic and mechanical properties. This study proposed a new strategy for reducing bacteria accumulation around the attachment, which is also promising in helping orthodontists to remove the attachment thoroughly and precisely.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>37015147</pmid><doi>10.1016/j.jmbbm.2023.105817</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-9234-0015</orcidid><orcidid>https://orcid.org/0000-0002-2438-5924</orcidid><orcidid>https://orcid.org/0000-0001-6409-6708</orcidid><orcidid>https://orcid.org/0000-0002-9733-6402</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1751-6161
ispartof Journal of the mechanical behavior of biomedical materials, 2023-05, Vol.141, p.105817-105817, Article 105817
issn 1751-6161
1878-0180
language eng
recordid cdi_proquest_miscellaneous_2796162331
source MEDLINE; Elsevier ScienceDirect Journals
subjects Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Antibacterial activity
Chlorhexidine
Chlorhexidine - chemistry
Chlorhexidine - pharmacology
Clear aligner attachment
Composite Resins - chemistry
Fluorescence
Materials Testing
Mesoporous silica nanoparticles
Methacrylates - chemistry
Nanoparticles - chemistry
Orthodontic Appliances, Removable
Quantum Dots
Silicon Dioxide - chemistry
Water
Zinc Oxide
Zinc oxide quantum dots
title Antibacterial and fluorescent clear aligner attachment resin modified with chlorhexidine loaded mesoporous silica nanoparticles and zinc oxide quantum dots
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-26T08%3A43%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Antibacterial%20and%20fluorescent%20clear%20aligner%20attachment%20resin%20modified%20with%20chlorhexidine%20loaded%20mesoporous%20silica%20nanoparticles%20and%20zinc%20oxide%20quantum%20dots&rft.jtitle=Journal%20of%20the%20mechanical%20behavior%20of%20biomedical%20materials&rft.au=Cao,%20Lingyun&rft.date=2023-05&rft.volume=141&rft.spage=105817&rft.epage=105817&rft.pages=105817-105817&rft.artnum=105817&rft.issn=1751-6161&rft.eissn=1878-0180&rft_id=info:doi/10.1016/j.jmbbm.2023.105817&rft_dat=%3Cproquest_cross%3E2796162331%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2796162331&rft_id=info:pmid/37015147&rft_els_id=S1751616123001704&rfr_iscdi=true