Application of PQQ-GDH Based Polymeric Layers in Design of Biosensors for Detection of Heavy Metals

A novel feature of biologically active polymer created by copolymerisation of glutaraldehyde (GA) with enzyme pyroloquinoline quinone dependent glucose dehydrogenase from Gluconobacter sp. 34 (PQQ-GDH) was its sensitivity to heavy metals. Examination of the influence of Cd2+ and Pb2+ ions on biologically active membranes was investigated. Both metal ions showed reversible inhibition of PQQ-GDH/GA copolymer. Inhibition effects of enzymatic activity of PQQ-dependent glucose dehydrogenase were applied in biosensors devoted for detection of Cd2+ and Pb 2+ ions. Basic characteristics of biosensors were determined. Here presented biosensor can be applied for detection of Cd2+ and Pb2+ ions in concentration range of 15 10-5 mol/l.