Ultrasensitive and simultaneous monitoring of multiple small-molecule pollutants on an immunochromatographic strip with multilayered film-like fluorescent tags
A wide variety of small-molecule pollutants is harmful to human health, and their highly sensitive universal and rapid detection in complex environments remains a challenge. Herein, a multiplexed and ultrasensitive immunochromatographic strip (ICS) was developed for the universal analysis of three k...
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| Veröffentlicht in: | The Science of the total environment 2023-06, Vol.878, p.162968-162968, Article 162968 |
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| creator | Wang, Wenqi Yu, Qing Zheng, Shuai Li, Jiaxuan Wu, Ting Wang, Shu Wang, Chongwen Gu, Bing |
| description | A wide variety of small-molecule pollutants is harmful to human health, and their highly sensitive universal and rapid detection in complex environments remains a challenge. Herein, a multiplexed and ultrasensitive immunochromatographic strip (ICS) was developed for the universal analysis of three kinds of different pollutants based on multilayered fluorescent nanofilm-guided signal amplification. Flexible three-dimensional nanofilms (GO–MQD) with large surface areas, high quantum dot (QD) loading, superior luminescence, and good stability were synthesized through the electrostatic adsorption-mediated layer-by-layer assembly of three layers of small QDs onto two-dimensional graphene oxide (GO) nanosheets, modified with specific antibodies, and utilized as enhanced fluorescent tags in the ICS method for quantitative target detection. By combining the GO–MQD nanofilms and multiplexed ICS, the proposed assay can rapidly and sensitively detect aflatoxin B1, clenbuterol, and kanamycin in actual samples/environmental samples (pork extract, milk, river water, and lake water) with low detection limit (0.87, 2.04, and 0.81 pg/mL), fast testing time (15 min), good stability and high reproducibility (RSD |
| doi_str_mv | 10.1016/j.scitotenv.2023.162968 |
| format | Article |
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[Display omitted]
•Film-like fluorescent tag was introduced into competitive immunochromatographic method for the first time.•Multilayer QDs were coated onto film-like tag to guide the signal amplification on strip.•Universal detection of different pollutants was first achieved on an immunochromatographic strip.•The LODs for small-molecule pollutants monitoring can reach pg/mL level.</description><identifier>ISSN: 0048-9697</identifier><identifier>EISSN: 1879-1026</identifier><identifier>DOI: 10.1016/j.scitotenv.2023.162968</identifier><identifier>PMID: 36958566</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Chromatography, Affinity - methods ; Fluorescent nanofilm ; Humans ; Immunochromatographic strip ; Limit of Detection ; Quantum dot ; Quantum Dots - chemistry ; Reproducibility of Results ; Small-molecule pollutants ; Universal detection ; Water</subject><ispartof>The Science of the total environment, 2023-06, Vol.878, p.162968-162968, Article 162968</ispartof><rights>2023</rights><rights>Copyright © 2023. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-36799c592fabbf4294a3d97515feabc06777efa21e8d78e24da6b5eb00866373</citedby><cites>FETCH-LOGICAL-c371t-36799c592fabbf4294a3d97515feabc06777efa21e8d78e24da6b5eb00866373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.scitotenv.2023.162968$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36958566$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Wenqi</creatorcontrib><creatorcontrib>Yu, Qing</creatorcontrib><creatorcontrib>Zheng, Shuai</creatorcontrib><creatorcontrib>Li, Jiaxuan</creatorcontrib><creatorcontrib>Wu, Ting</creatorcontrib><creatorcontrib>Wang, Shu</creatorcontrib><creatorcontrib>Wang, Chongwen</creatorcontrib><creatorcontrib>Gu, Bing</creatorcontrib><title>Ultrasensitive and simultaneous monitoring of multiple small-molecule pollutants on an immunochromatographic strip with multilayered film-like fluorescent tags</title><title>The Science of the total environment</title><addtitle>Sci Total Environ</addtitle><description>A wide variety of small-molecule pollutants is harmful to human health, and their highly sensitive universal and rapid detection in complex environments remains a challenge. Herein, a multiplexed and ultrasensitive immunochromatographic strip (ICS) was developed for the universal analysis of three kinds of different pollutants based on multilayered fluorescent nanofilm-guided signal amplification. Flexible three-dimensional nanofilms (GO–MQD) with large surface areas, high quantum dot (QD) loading, superior luminescence, and good stability were synthesized through the electrostatic adsorption-mediated layer-by-layer assembly of three layers of small QDs onto two-dimensional graphene oxide (GO) nanosheets, modified with specific antibodies, and utilized as enhanced fluorescent tags in the ICS method for quantitative target detection. By combining the GO–MQD nanofilms and multiplexed ICS, the proposed assay can rapidly and sensitively detect aflatoxin B1, clenbuterol, and kanamycin in actual samples/environmental samples (pork extract, milk, river water, and lake water) with low detection limit (0.87, 2.04, and 0.81 pg/mL), fast testing time (15 min), good stability and high reproducibility (RSD < 8.71 %). The GO–MQD–ICS method developed here exhibits great potential to meet the demands of the on-site and practical detection of small-molecule pollutants.
[Display omitted]
•Film-like fluorescent tag was introduced into competitive immunochromatographic method for the first time.•Multilayer QDs were coated onto film-like tag to guide the signal amplification on strip.•Universal detection of different pollutants was first achieved on an immunochromatographic strip.•The LODs for small-molecule pollutants monitoring can reach pg/mL level.</description><subject>Chromatography, Affinity - methods</subject><subject>Fluorescent nanofilm</subject><subject>Humans</subject><subject>Immunochromatographic strip</subject><subject>Limit of Detection</subject><subject>Quantum dot</subject><subject>Quantum Dots - chemistry</subject><subject>Reproducibility of Results</subject><subject>Small-molecule pollutants</subject><subject>Universal detection</subject><subject>Water</subject><issn>0048-9697</issn><issn>1879-1026</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u3CAUhVHVqJkmeYWWZTeeAh6DWUZR01aK1E2yRhhfzzDlxwU8VZ6mr1pGTrMtG4T4zjkXDkIfKdlSQvnn4zYbW2KBcNoywtot5Uzy_g3a0F7IhhLG36INIbu-kVyKS_Q-5yOpS_T0Hbpsuez6jvMN-vPkStIZQrbFngDrMOJs_eKKDhCXjH0MNSjZsMdxwucLOzvA2WvnGh8dmKUe5-jcUiUl4xiqCbbeLyGaQ4pel7hPej5Yg3NJdsa_bTmsTk4_Q4IRT9b5xtmfgCe3xATZQCi46H2-RheTdhluXvYr9Hj_5fHuW_Pw4-v3u9uHxrSClqblQkrTSTbpYZh2TO50O0rR0W4CPRjChRAwaUahH0UPbDdqPnQwENJz3or2Cn1abecUfy2Qi_K2DuHc-guKCUkrxmlbUbGiJsWcE0xqTtbr9KwoUedy1FG9lqPO5ai1nKr88BKyDB7GV92_NipwuwJQX3qykM5GEAyMNoEpaoz2vyF_AdZVq50</recordid><startdate>20230620</startdate><enddate>20230620</enddate><creator>Wang, Wenqi</creator><creator>Yu, Qing</creator><creator>Zheng, Shuai</creator><creator>Li, Jiaxuan</creator><creator>Wu, Ting</creator><creator>Wang, Shu</creator><creator>Wang, Chongwen</creator><creator>Gu, Bing</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20230620</creationdate><title>Ultrasensitive and simultaneous monitoring of multiple small-molecule pollutants on an immunochromatographic strip with multilayered film-like fluorescent tags</title><author>Wang, Wenqi ; Yu, Qing ; Zheng, Shuai ; Li, Jiaxuan ; Wu, Ting ; Wang, Shu ; Wang, Chongwen ; Gu, Bing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-36799c592fabbf4294a3d97515feabc06777efa21e8d78e24da6b5eb00866373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Chromatography, Affinity - methods</topic><topic>Fluorescent nanofilm</topic><topic>Humans</topic><topic>Immunochromatographic strip</topic><topic>Limit of Detection</topic><topic>Quantum dot</topic><topic>Quantum Dots - chemistry</topic><topic>Reproducibility of Results</topic><topic>Small-molecule pollutants</topic><topic>Universal detection</topic><topic>Water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Wenqi</creatorcontrib><creatorcontrib>Yu, Qing</creatorcontrib><creatorcontrib>Zheng, Shuai</creatorcontrib><creatorcontrib>Li, Jiaxuan</creatorcontrib><creatorcontrib>Wu, Ting</creatorcontrib><creatorcontrib>Wang, Shu</creatorcontrib><creatorcontrib>Wang, Chongwen</creatorcontrib><creatorcontrib>Gu, Bing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Science of the total environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Wenqi</au><au>Yu, Qing</au><au>Zheng, Shuai</au><au>Li, Jiaxuan</au><au>Wu, Ting</au><au>Wang, Shu</au><au>Wang, Chongwen</au><au>Gu, Bing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrasensitive and simultaneous monitoring of multiple small-molecule pollutants on an immunochromatographic strip with multilayered film-like fluorescent tags</atitle><jtitle>The Science of the total environment</jtitle><addtitle>Sci Total Environ</addtitle><date>2023-06-20</date><risdate>2023</risdate><volume>878</volume><spage>162968</spage><epage>162968</epage><pages>162968-162968</pages><artnum>162968</artnum><issn>0048-9697</issn><eissn>1879-1026</eissn><abstract>A wide variety of small-molecule pollutants is harmful to human health, and their highly sensitive universal and rapid detection in complex environments remains a challenge. Herein, a multiplexed and ultrasensitive immunochromatographic strip (ICS) was developed for the universal analysis of three kinds of different pollutants based on multilayered fluorescent nanofilm-guided signal amplification. Flexible three-dimensional nanofilms (GO–MQD) with large surface areas, high quantum dot (QD) loading, superior luminescence, and good stability were synthesized through the electrostatic adsorption-mediated layer-by-layer assembly of three layers of small QDs onto two-dimensional graphene oxide (GO) nanosheets, modified with specific antibodies, and utilized as enhanced fluorescent tags in the ICS method for quantitative target detection. By combining the GO–MQD nanofilms and multiplexed ICS, the proposed assay can rapidly and sensitively detect aflatoxin B1, clenbuterol, and kanamycin in actual samples/environmental samples (pork extract, milk, river water, and lake water) with low detection limit (0.87, 2.04, and 0.81 pg/mL), fast testing time (15 min), good stability and high reproducibility (RSD < 8.71 %). The GO–MQD–ICS method developed here exhibits great potential to meet the demands of the on-site and practical detection of small-molecule pollutants.
[Display omitted]
•Film-like fluorescent tag was introduced into competitive immunochromatographic method for the first time.•Multilayer QDs were coated onto film-like tag to guide the signal amplification on strip.•Universal detection of different pollutants was first achieved on an immunochromatographic strip.•The LODs for small-molecule pollutants monitoring can reach pg/mL level.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>36958566</pmid><doi>10.1016/j.scitotenv.2023.162968</doi><tpages>1</tpages></addata></record> |
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| subjects | Chromatography, Affinity - methods Fluorescent nanofilm Humans Immunochromatographic strip Limit of Detection Quantum dot Quantum Dots - chemistry Reproducibility of Results Small-molecule pollutants Universal detection Water |
| title | Ultrasensitive and simultaneous monitoring of multiple small-molecule pollutants on an immunochromatographic strip with multilayered film-like fluorescent tags |
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