High potency of magnetic iron oxide nanoparticles covered by piroctone olamine against cystic echinococcosis
This study examined the synthesis of magnetic iron oxide nanoparticles coated with PO (FOMNPsP) and assessed their in vitro, ex vivo, and in vivo effects against cystic echinococcosis. The FOMNPsP was synthesized through the alkalization of iron ions in a deoxygenated form. In vitro and ex vivo prot...
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| Veröffentlicht in: | Biomedicine & pharmacotherapy 2023-05, Vol.161, p.114536-114536, Article 114536 |
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| container_title | Biomedicine & pharmacotherapy |
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| creator | Raziani, Yosra Cheraghipour, Koroush Shakibaie, Mojtaba Ghasemian Yadegari, Javad Mahmoudvand, Hossein |
| description | This study examined the synthesis of magnetic iron oxide nanoparticles coated with PO (FOMNPsP) and assessed their in vitro, ex vivo, and in vivo effects against cystic echinococcosis. The FOMNPsP was synthesized through the alkalization of iron ions in a deoxygenated form. In vitro and ex vivo protoscolicidal effects of FOMNPsP (100–400 μg/mL) were evaluated on hydatid cyst protoscoleces by the eosin exclusion test for 10–60 min. The effect of FOMNPsP on caspase-3 gene expression and exterior ultra-structural of protoscoleces was assessed by real-time PCR and scanning electron microscopy (SEM), respectively. In vivo effects were assessed by evaluating the number, size, and weight of hydatid cysts among infected mice. The FOMNPsP size was |
| doi_str_mv | 10.1016/j.biopha.2023.114536 |
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The FOMNPsP was synthesized through the alkalization of iron ions in a deoxygenated form. In vitro and ex vivo protoscolicidal effects of FOMNPsP (100–400 μg/mL) were evaluated on hydatid cyst protoscoleces by the eosin exclusion test for 10–60 min. The effect of FOMNPsP on caspase-3 gene expression and exterior ultra-structural of protoscoleces was assessed by real-time PCR and scanning electron microscopy (SEM), respectively. In vivo effects were assessed by evaluating the number, size, and weight of hydatid cysts among infected mice. The FOMNPsP size was < 55 nm, and the most frequent particles were in the 15–20 nm range. In vitro and ex vivo assays revealed that the highest protoscolicidal effect was observed at 400 µg/mL with 100% lethality. After exposure of protoscoleces with FOMNPsP, the level of gene expression of caspase-3 was dose-dependently increased (p < 0.05). By SEM, the FOMNPsP-treated protoscoleces showed wrinkles and bulges resulting from the formation of blebs. FOMNPsP significantly decreased (p < 0.01) the mean number, size, and weight of the hydatid cyst. FOMNPsP revealed the potent protoscolicidal traits through disrupting the cell wall and apoptosis induction. The results also indicated the promising effect of FOMNPsP in controlling hydatid cysts in the animal model. Although FOMNPsP is safe for human normal cells, more investigations are required to clarify its toxicity and precise mechanisms of action.</description><identifier>ISSN: 0753-3322</identifier><identifier>EISSN: 1950-6007</identifier><identifier>DOI: 10.1016/j.biopha.2023.114536</identifier><identifier>PMID: 36940617</identifier><language>eng</language><publisher>France: Elsevier Masson SAS</publisher><subject>Animals ; Apoptosis ; Caspase 3 ; Echinococcosis - drug therapy ; Echinococcus ; Humans ; Hydatid cyst ; Magnetic Iron Oxide Nanoparticles ; Mice ; Nanomedicine ; Protoscolicidal ; Scolicidal</subject><ispartof>Biomedicine & pharmacotherapy, 2023-05, Vol.161, p.114536-114536, Article 114536</ispartof><rights>2023 The Authors</rights><rights>Copyright © 2023 The Authors. Published by Elsevier Masson SAS.. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-cc48b392bfaf86accdf9bb1879793208010b56eff41afa4bba91db2fbb4d40393</citedby><cites>FETCH-LOGICAL-c408t-cc48b392bfaf86accdf9bb1879793208010b56eff41afa4bba91db2fbb4d40393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biopha.2023.114536$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36940617$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Raziani, Yosra</creatorcontrib><creatorcontrib>Cheraghipour, Koroush</creatorcontrib><creatorcontrib>Shakibaie, Mojtaba</creatorcontrib><creatorcontrib>Ghasemian Yadegari, Javad</creatorcontrib><creatorcontrib>Mahmoudvand, Hossein</creatorcontrib><title>High potency of magnetic iron oxide nanoparticles covered by piroctone olamine against cystic echinococcosis</title><title>Biomedicine & pharmacotherapy</title><addtitle>Biomed Pharmacother</addtitle><description>This study examined the synthesis of magnetic iron oxide nanoparticles coated with PO (FOMNPsP) and assessed their in vitro, ex vivo, and in vivo effects against cystic echinococcosis. The FOMNPsP was synthesized through the alkalization of iron ions in a deoxygenated form. In vitro and ex vivo protoscolicidal effects of FOMNPsP (100–400 μg/mL) were evaluated on hydatid cyst protoscoleces by the eosin exclusion test for 10–60 min. The effect of FOMNPsP on caspase-3 gene expression and exterior ultra-structural of protoscoleces was assessed by real-time PCR and scanning electron microscopy (SEM), respectively. In vivo effects were assessed by evaluating the number, size, and weight of hydatid cysts among infected mice. The FOMNPsP size was < 55 nm, and the most frequent particles were in the 15–20 nm range. In vitro and ex vivo assays revealed that the highest protoscolicidal effect was observed at 400 µg/mL with 100% lethality. After exposure of protoscoleces with FOMNPsP, the level of gene expression of caspase-3 was dose-dependently increased (p < 0.05). By SEM, the FOMNPsP-treated protoscoleces showed wrinkles and bulges resulting from the formation of blebs. FOMNPsP significantly decreased (p < 0.01) the mean number, size, and weight of the hydatid cyst. FOMNPsP revealed the potent protoscolicidal traits through disrupting the cell wall and apoptosis induction. The results also indicated the promising effect of FOMNPsP in controlling hydatid cysts in the animal model. Although FOMNPsP is safe for human normal cells, more investigations are required to clarify its toxicity and precise mechanisms of action.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Caspase 3</subject><subject>Echinococcosis - drug therapy</subject><subject>Echinococcus</subject><subject>Humans</subject><subject>Hydatid cyst</subject><subject>Magnetic Iron Oxide Nanoparticles</subject><subject>Mice</subject><subject>Nanomedicine</subject><subject>Protoscolicidal</subject><subject>Scolicidal</subject><issn>0753-3322</issn><issn>1950-6007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhi1ERZfCP0DIRy5Z_JUPX5BQBS1SJS5wtuzJeNerxA52tmL_fROl5cjplUbPzKt5CPnA2Z4z3nw-7V1I09HuBRNyz7mqZfOK7LiuWdUw1r4mO9bWspJSiGvytpQTY6xuZPeGXMtGK9bwdkeG-3A40inNGOFCk6ejPUScA9CQU6Tpb-iRRhvTZPMyHbBQSI-YsafuQqcFgjlFpGmwY1jSHmyIZaZwKesRhGOICRJAKqG8I1feDgXfP-cN-f3926_b--rh592P268PFSjWzRWA6pzUwnnru8YC9F47x7tWt1oK1jHOXN2g94pbb5VzVvPeCe-c6hWTWt6QT9vdKac_ZyyzGUMBHAYbMZ2LEW2nxepgRdWGQk6lZPRmymG0-WI4M6tnczKbZ7N6NpvnZe3jc8PZjdj_W3oRuwBfNgCXPx8DZlMgLI6xDxlhNn0K_294AiQhk1A</recordid><startdate>202305</startdate><enddate>202305</enddate><creator>Raziani, Yosra</creator><creator>Cheraghipour, Koroush</creator><creator>Shakibaie, Mojtaba</creator><creator>Ghasemian Yadegari, Javad</creator><creator>Mahmoudvand, Hossein</creator><general>Elsevier Masson SAS</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202305</creationdate><title>High potency of magnetic iron oxide nanoparticles covered by piroctone olamine against cystic echinococcosis</title><author>Raziani, Yosra ; Cheraghipour, Koroush ; Shakibaie, Mojtaba ; Ghasemian Yadegari, Javad ; Mahmoudvand, Hossein</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-cc48b392bfaf86accdf9bb1879793208010b56eff41afa4bba91db2fbb4d40393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Caspase 3</topic><topic>Echinococcosis - drug therapy</topic><topic>Echinococcus</topic><topic>Humans</topic><topic>Hydatid cyst</topic><topic>Magnetic Iron Oxide Nanoparticles</topic><topic>Mice</topic><topic>Nanomedicine</topic><topic>Protoscolicidal</topic><topic>Scolicidal</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Raziani, Yosra</creatorcontrib><creatorcontrib>Cheraghipour, Koroush</creatorcontrib><creatorcontrib>Shakibaie, Mojtaba</creatorcontrib><creatorcontrib>Ghasemian Yadegari, Javad</creatorcontrib><creatorcontrib>Mahmoudvand, Hossein</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedicine & pharmacotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Raziani, Yosra</au><au>Cheraghipour, Koroush</au><au>Shakibaie, Mojtaba</au><au>Ghasemian Yadegari, Javad</au><au>Mahmoudvand, Hossein</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High potency of magnetic iron oxide nanoparticles covered by piroctone olamine against cystic echinococcosis</atitle><jtitle>Biomedicine & pharmacotherapy</jtitle><addtitle>Biomed Pharmacother</addtitle><date>2023-05</date><risdate>2023</risdate><volume>161</volume><spage>114536</spage><epage>114536</epage><pages>114536-114536</pages><artnum>114536</artnum><issn>0753-3322</issn><eissn>1950-6007</eissn><abstract>This study examined the synthesis of magnetic iron oxide nanoparticles coated with PO (FOMNPsP) and assessed their in vitro, ex vivo, and in vivo effects against cystic echinococcosis. The FOMNPsP was synthesized through the alkalization of iron ions in a deoxygenated form. In vitro and ex vivo protoscolicidal effects of FOMNPsP (100–400 μg/mL) were evaluated on hydatid cyst protoscoleces by the eosin exclusion test for 10–60 min. The effect of FOMNPsP on caspase-3 gene expression and exterior ultra-structural of protoscoleces was assessed by real-time PCR and scanning electron microscopy (SEM), respectively. In vivo effects were assessed by evaluating the number, size, and weight of hydatid cysts among infected mice. The FOMNPsP size was < 55 nm, and the most frequent particles were in the 15–20 nm range. In vitro and ex vivo assays revealed that the highest protoscolicidal effect was observed at 400 µg/mL with 100% lethality. After exposure of protoscoleces with FOMNPsP, the level of gene expression of caspase-3 was dose-dependently increased (p < 0.05). By SEM, the FOMNPsP-treated protoscoleces showed wrinkles and bulges resulting from the formation of blebs. FOMNPsP significantly decreased (p < 0.01) the mean number, size, and weight of the hydatid cyst. FOMNPsP revealed the potent protoscolicidal traits through disrupting the cell wall and apoptosis induction. The results also indicated the promising effect of FOMNPsP in controlling hydatid cysts in the animal model. Although FOMNPsP is safe for human normal cells, more investigations are required to clarify its toxicity and precise mechanisms of action.</abstract><cop>France</cop><pub>Elsevier Masson SAS</pub><pmid>36940617</pmid><doi>10.1016/j.biopha.2023.114536</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Apoptosis Caspase 3 Echinococcosis - drug therapy Echinococcus Humans Hydatid cyst Magnetic Iron Oxide Nanoparticles Mice Nanomedicine Protoscolicidal Scolicidal |
| title | High potency of magnetic iron oxide nanoparticles covered by piroctone olamine against cystic echinococcosis |
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