A comparison of post-ruminal provision of Ca-gluconate and Ca-butyrate on growth performance, gastrointestinal barrier function, short-chain fatty acid absorption, intestinal histology, and brush-border enzyme activity in beef heifers
Abstract The objective of this study was to compare the effects of post-ruminal provision of Ca-butyrate (CaB) when delivered via abomasal dosing, and Ca-gluconate (CaG) when provided ruminally using a rumen protected form or using an unprotected form via abomasal dosing on short-chain fatty acid (S...
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| creator | Watanabe, Daniel H M Doelman, John Steele, Michael A Guan, Le L Seymour, Dave J Penner, Gregory B |
| description | Abstract
The objective of this study was to compare the effects of post-ruminal provision of Ca-butyrate (CaB) when delivered via abomasal dosing, and Ca-gluconate (CaG) when provided ruminally using a rumen protected form or using an unprotected form via abomasal dosing on short-chain fatty acid (SCFA) concentration throughout the GIT, nutrient digestibility, GIT barrier function, ruminal SCFA absorption, ruminal morphometrics, intestinal brush border enzyme activity, and blood parameters for beef heifers. Thirty-two beef heifers fitted with ruminal cannulas were used in a randomized complete block design and assigned to one of four treatments: 1) negative control (ruminal infusion of double-distilled water; CON); 2) abomasal infusion of CaB (AB; 0.0029% of BW); 3) abomasal infusion of CaG (AG; 0.0077% of BW); and 4) ruminal infusion of a hydrogenated fat-embedded CaG (RG; 0.0192% of BW) to provide ruminal protection. Excluding CON, treatments were designed to deliver the same amount of butyrate in the small intestine. Heifers were housed in individual pens and DMI was limited to 95% of voluntary intake to minimize a potential confounding effect of DMI on treatment responses. Total GIT barrier function was assessed on day 17 and SCFA disappearance was evaluated on day 21 using the temporarily isolated and washed reticulo-rumen technique. On day 28, heifers were slaughtered, and ruminal and colonic digesta were collected to assess SCFA concentration. Additionally, ruminal, jejunal, and colonic tissues were collected to assess SCFA fluxes and regional barrier function ex vivo using the Ussing chamber technique. For colonic digesta, both AB and CaG treatments reduced the proportion of acetate (P < 0.05) and increased the proportion on propionate (P < 0.05) compared to CON. Relative to CON, AB but not CaG treatments increased in vivo ruminal disappearance of total SCFA (P = 0.01), acetate (P = 0.03), propionate (P = 0.01), and butyrate (P > 0.01). Treatments did not affect (P ≥ 0.10) acetate and butyrate fluxes in the ruminal and colonic tissues when measured ex vivo; however, when compared with CON, AB tended to decrease (P = 0.09) mannitol flux across ruminal tissue. In addition, mannitol flux was affected (P < 0.01) by region, with greater mannitol flux across the jejunum than rumen and colon. We conclude that while both abomasal infusion of CaB and CaG affect the molar proportion of acetate and propionate in the colon, only abomasal CaB stimulated ruminal |
| doi_str_mv | 10.1093/jas/skad050 |
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The objective of this study was to compare the effects of post-ruminal provision of Ca-butyrate (CaB) when delivered via abomasal dosing, and Ca-gluconate (CaG) when provided ruminally using a rumen protected form or using an unprotected form via abomasal dosing on short-chain fatty acid (SCFA) concentration throughout the GIT, nutrient digestibility, GIT barrier function, ruminal SCFA absorption, ruminal morphometrics, intestinal brush border enzyme activity, and blood parameters for beef heifers. Thirty-two beef heifers fitted with ruminal cannulas were used in a randomized complete block design and assigned to one of four treatments: 1) negative control (ruminal infusion of double-distilled water; CON); 2) abomasal infusion of CaB (AB; 0.0029% of BW); 3) abomasal infusion of CaG (AG; 0.0077% of BW); and 4) ruminal infusion of a hydrogenated fat-embedded CaG (RG; 0.0192% of BW) to provide ruminal protection. Excluding CON, treatments were designed to deliver the same amount of butyrate in the small intestine. Heifers were housed in individual pens and DMI was limited to 95% of voluntary intake to minimize a potential confounding effect of DMI on treatment responses. Total GIT barrier function was assessed on day 17 and SCFA disappearance was evaluated on day 21 using the temporarily isolated and washed reticulo-rumen technique. On day 28, heifers were slaughtered, and ruminal and colonic digesta were collected to assess SCFA concentration. Additionally, ruminal, jejunal, and colonic tissues were collected to assess SCFA fluxes and regional barrier function ex vivo using the Ussing chamber technique. For colonic digesta, both AB and CaG treatments reduced the proportion of acetate (P < 0.05) and increased the proportion on propionate (P < 0.05) compared to CON. Relative to CON, AB but not CaG treatments increased in vivo ruminal disappearance of total SCFA (P = 0.01), acetate (P = 0.03), propionate (P = 0.01), and butyrate (P > 0.01). Treatments did not affect (P ≥ 0.10) acetate and butyrate fluxes in the ruminal and colonic tissues when measured ex vivo; however, when compared with CON, AB tended to decrease (P = 0.09) mannitol flux across ruminal tissue. In addition, mannitol flux was affected (P < 0.01) by region, with greater mannitol flux across the jejunum than rumen and colon. We conclude that while both abomasal infusion of CaB and CaG affect the molar proportion of acetate and propionate in the colon, only abomasal CaB stimulated ruminal SCFA absorption for growing beef heifers.
Butyrate supplementation has been proven to enhance gastrointestinal tract development and functionality. This study evaluates the mode of action and the effects of Ca-gluconate relative to butyrate on gastrointestinal tract function in beef heifers.
Lay Summary
Butyrate, a short-chain fatty acid (SCFA), has received attention due to its ability to promote gastrointestinal (GIT) health and development. However, butyrate in its free form presents a strong odor, limiting its use in diet formulation. Supplementation of butyrate precursors, such as gluconate, have been studied to enhance butyrate production in the GIT. This study evaluated the effects of post-ruminal infusion of Ca-butyrate (AB; 0.0029% of BW) and Ca-gluconate (AG; 0.0077% of BW) and ruminal infusion of a hydrogenated fat-embedded Ca-gluconate (RG; 0.0192% of BW) relative to control (CON; ruminal infusion of double-distilled water). Thirty-two beef heifers fitted with ruminal cannulas were fed for 28 d and GIT barrier function and ruminal SCFA absorption were assessed. At slaughter, the rumen, jejunum, and colon tissues were collected and barrier function and SCFA fluxes were assessed ex vivo. Relative to CON, AB but not AG and RG increased in vivo ruminal SCFA absorption and tended to increase ex vivo barrier function. Thus, the data presented in this study shows that butyrate and gluconate do not function through the same mode of action in the GIT of beef heifers.</description><identifier>ISSN: 0021-8812</identifier><identifier>EISSN: 1525-3163</identifier><identifier>DOI: 10.1093/jas/skad050</identifier><identifier>PMID: 36799118</identifier><language>eng</language><publisher>US: Oxford University Press</publisher><subject>Absorption ; Acetic acid ; Animal Feed - analysis ; Animals ; Beef ; Butyrates - metabolism ; Butyrates - pharmacology ; Cattle ; Colon ; Diet - veterinary ; Digestibility ; Digestion - physiology ; Distilled water ; Dosage ; Enzymatic activity ; Enzyme activity ; Enzymes ; Fatty acids ; Fatty Acids, Volatile - metabolism ; Female ; Fermentation ; Fluctuations ; Fluxes ; Gluconates - metabolism ; Histology ; Intestinal Absorption ; Intestine ; Jejunum ; Mannitol ; Microvilli ; Morphometry ; Nutrient concentrations ; Propionates - metabolism ; Propionic acid ; Rumen ; Rumen - metabolism ; Small intestine ; Trinucleotide repeats</subject><ispartof>Journal of animal science, 2023-01, Vol.101</ispartof><rights>The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com. 2023</rights><rights>The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c348t-d4cfe149852dbc7688aa2dba75a7d538b233bccbcd1507fa94542d17fb705aa93</citedby><cites>FETCH-LOGICAL-c348t-d4cfe149852dbc7688aa2dba75a7d538b233bccbcd1507fa94542d17fb705aa93</cites><orcidid>0000-0001-6208-8720 ; 0000-0002-6396-2130</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1584,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36799118$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Watanabe, Daniel H M</creatorcontrib><creatorcontrib>Doelman, John</creatorcontrib><creatorcontrib>Steele, Michael A</creatorcontrib><creatorcontrib>Guan, Le L</creatorcontrib><creatorcontrib>Seymour, Dave J</creatorcontrib><creatorcontrib>Penner, Gregory B</creatorcontrib><title>A comparison of post-ruminal provision of Ca-gluconate and Ca-butyrate on growth performance, gastrointestinal barrier function, short-chain fatty acid absorption, intestinal histology, and brush-border enzyme activity in beef heifers</title><title>Journal of animal science</title><addtitle>J Anim Sci</addtitle><description>Abstract
The objective of this study was to compare the effects of post-ruminal provision of Ca-butyrate (CaB) when delivered via abomasal dosing, and Ca-gluconate (CaG) when provided ruminally using a rumen protected form or using an unprotected form via abomasal dosing on short-chain fatty acid (SCFA) concentration throughout the GIT, nutrient digestibility, GIT barrier function, ruminal SCFA absorption, ruminal morphometrics, intestinal brush border enzyme activity, and blood parameters for beef heifers. Thirty-two beef heifers fitted with ruminal cannulas were used in a randomized complete block design and assigned to one of four treatments: 1) negative control (ruminal infusion of double-distilled water; CON); 2) abomasal infusion of CaB (AB; 0.0029% of BW); 3) abomasal infusion of CaG (AG; 0.0077% of BW); and 4) ruminal infusion of a hydrogenated fat-embedded CaG (RG; 0.0192% of BW) to provide ruminal protection. Excluding CON, treatments were designed to deliver the same amount of butyrate in the small intestine. Heifers were housed in individual pens and DMI was limited to 95% of voluntary intake to minimize a potential confounding effect of DMI on treatment responses. Total GIT barrier function was assessed on day 17 and SCFA disappearance was evaluated on day 21 using the temporarily isolated and washed reticulo-rumen technique. On day 28, heifers were slaughtered, and ruminal and colonic digesta were collected to assess SCFA concentration. Additionally, ruminal, jejunal, and colonic tissues were collected to assess SCFA fluxes and regional barrier function ex vivo using the Ussing chamber technique. For colonic digesta, both AB and CaG treatments reduced the proportion of acetate (P < 0.05) and increased the proportion on propionate (P < 0.05) compared to CON. Relative to CON, AB but not CaG treatments increased in vivo ruminal disappearance of total SCFA (P = 0.01), acetate (P = 0.03), propionate (P = 0.01), and butyrate (P > 0.01). Treatments did not affect (P ≥ 0.10) acetate and butyrate fluxes in the ruminal and colonic tissues when measured ex vivo; however, when compared with CON, AB tended to decrease (P = 0.09) mannitol flux across ruminal tissue. In addition, mannitol flux was affected (P < 0.01) by region, with greater mannitol flux across the jejunum than rumen and colon. We conclude that while both abomasal infusion of CaB and CaG affect the molar proportion of acetate and propionate in the colon, only abomasal CaB stimulated ruminal SCFA absorption for growing beef heifers.
Butyrate supplementation has been proven to enhance gastrointestinal tract development and functionality. This study evaluates the mode of action and the effects of Ca-gluconate relative to butyrate on gastrointestinal tract function in beef heifers.
Lay Summary
Butyrate, a short-chain fatty acid (SCFA), has received attention due to its ability to promote gastrointestinal (GIT) health and development. However, butyrate in its free form presents a strong odor, limiting its use in diet formulation. Supplementation of butyrate precursors, such as gluconate, have been studied to enhance butyrate production in the GIT. This study evaluated the effects of post-ruminal infusion of Ca-butyrate (AB; 0.0029% of BW) and Ca-gluconate (AG; 0.0077% of BW) and ruminal infusion of a hydrogenated fat-embedded Ca-gluconate (RG; 0.0192% of BW) relative to control (CON; ruminal infusion of double-distilled water). Thirty-two beef heifers fitted with ruminal cannulas were fed for 28 d and GIT barrier function and ruminal SCFA absorption were assessed. At slaughter, the rumen, jejunum, and colon tissues were collected and barrier function and SCFA fluxes were assessed ex vivo. Relative to CON, AB but not AG and RG increased in vivo ruminal SCFA absorption and tended to increase ex vivo barrier function. Thus, the data presented in this study shows that butyrate and gluconate do not function through the same mode of action in the GIT of beef heifers.</description><subject>Absorption</subject><subject>Acetic acid</subject><subject>Animal Feed - analysis</subject><subject>Animals</subject><subject>Beef</subject><subject>Butyrates - metabolism</subject><subject>Butyrates - pharmacology</subject><subject>Cattle</subject><subject>Colon</subject><subject>Diet - veterinary</subject><subject>Digestibility</subject><subject>Digestion - physiology</subject><subject>Distilled water</subject><subject>Dosage</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Enzymes</subject><subject>Fatty acids</subject><subject>Fatty Acids, Volatile - metabolism</subject><subject>Female</subject><subject>Fermentation</subject><subject>Fluctuations</subject><subject>Fluxes</subject><subject>Gluconates - metabolism</subject><subject>Histology</subject><subject>Intestinal Absorption</subject><subject>Intestine</subject><subject>Jejunum</subject><subject>Mannitol</subject><subject>Microvilli</subject><subject>Morphometry</subject><subject>Nutrient concentrations</subject><subject>Propionates - metabolism</subject><subject>Propionic acid</subject><subject>Rumen</subject><subject>Rumen - metabolism</subject><subject>Small intestine</subject><subject>Trinucleotide repeats</subject><issn>0021-8812</issn><issn>1525-3163</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kk-P1CAYhxujccfVk3dDYmJMnLpQypQeNxP_JZt40XPzQmHK2Jb6QndTP7KfQmY7GuPBE_Dy8OQHvFn2nNG3jNb86gjhKnyDlgr6INswUYicsx1_mG0oLVguJSsusichHCllhajF4-yC76q6Zkxusp_XRPthAnTBj8RbMvkQc5wHN0JPJvS3Lrh1Zw_5oZ-1HyEaAmN7Kqg5LnhaJ-SA_i52ZDJoPQ4warMlBwgRvRujCfHeqADRGSR2HnVM4i0JnceY6w7cSCzEuBDQriWggsdpRf4637kQfe8Py_Y-gsI5dLny2CanGX8sQ4qWxLcueZJQGWNJZ5w1GJ5mjyz0wTw7j5fZ1_fvvuw_5jefP3zaX9_kmpcy5m2prWFlLUXRKl3tpARIM6gEVK3gUhWcK62VbpmglYW6FGXRssqqigqAml9mr1dver3vcwreDC5o0_cwGj-HpqiklJTuyl1CX_6DHv2M6Z6h4bRkVHBOT9SbldLoQ0BjmwndALg0jDanFmhSCzTnFkj0i7NzVoNp_7C__zwBr1bAz9N_Tb8Aa6HBxQ</recordid><startdate>20230103</startdate><enddate>20230103</enddate><creator>Watanabe, Daniel H M</creator><creator>Doelman, John</creator><creator>Steele, Michael A</creator><creator>Guan, Le L</creator><creator>Seymour, Dave J</creator><creator>Penner, Gregory B</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>U9A</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6208-8720</orcidid><orcidid>https://orcid.org/0000-0002-6396-2130</orcidid></search><sort><creationdate>20230103</creationdate><title>A comparison of post-ruminal provision of Ca-gluconate and Ca-butyrate on growth performance, gastrointestinal barrier function, short-chain fatty acid absorption, intestinal histology, and brush-border enzyme activity in beef heifers</title><author>Watanabe, Daniel H M ; Doelman, John ; Steele, Michael A ; Guan, Le L ; Seymour, Dave J ; Penner, Gregory B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-d4cfe149852dbc7688aa2dba75a7d538b233bccbcd1507fa94542d17fb705aa93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Absorption</topic><topic>Acetic acid</topic><topic>Animal Feed - analysis</topic><topic>Animals</topic><topic>Beef</topic><topic>Butyrates - metabolism</topic><topic>Butyrates - pharmacology</topic><topic>Cattle</topic><topic>Colon</topic><topic>Diet - veterinary</topic><topic>Digestibility</topic><topic>Digestion - physiology</topic><topic>Distilled water</topic><topic>Dosage</topic><topic>Enzymatic activity</topic><topic>Enzyme activity</topic><topic>Enzymes</topic><topic>Fatty acids</topic><topic>Fatty Acids, Volatile - metabolism</topic><topic>Female</topic><topic>Fermentation</topic><topic>Fluctuations</topic><topic>Fluxes</topic><topic>Gluconates - metabolism</topic><topic>Histology</topic><topic>Intestinal Absorption</topic><topic>Intestine</topic><topic>Jejunum</topic><topic>Mannitol</topic><topic>Microvilli</topic><topic>Morphometry</topic><topic>Nutrient concentrations</topic><topic>Propionates - metabolism</topic><topic>Propionic acid</topic><topic>Rumen</topic><topic>Rumen - metabolism</topic><topic>Small intestine</topic><topic>Trinucleotide repeats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Watanabe, Daniel H M</creatorcontrib><creatorcontrib>Doelman, John</creatorcontrib><creatorcontrib>Steele, Michael A</creatorcontrib><creatorcontrib>Guan, Le L</creatorcontrib><creatorcontrib>Seymour, Dave J</creatorcontrib><creatorcontrib>Penner, Gregory B</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of animal science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Watanabe, Daniel H M</au><au>Doelman, John</au><au>Steele, Michael A</au><au>Guan, Le L</au><au>Seymour, Dave J</au><au>Penner, Gregory B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A comparison of post-ruminal provision of Ca-gluconate and Ca-butyrate on growth performance, gastrointestinal barrier function, short-chain fatty acid absorption, intestinal histology, and brush-border enzyme activity in beef heifers</atitle><jtitle>Journal of animal science</jtitle><addtitle>J Anim Sci</addtitle><date>2023-01-03</date><risdate>2023</risdate><volume>101</volume><issn>0021-8812</issn><eissn>1525-3163</eissn><abstract>Abstract
The objective of this study was to compare the effects of post-ruminal provision of Ca-butyrate (CaB) when delivered via abomasal dosing, and Ca-gluconate (CaG) when provided ruminally using a rumen protected form or using an unprotected form via abomasal dosing on short-chain fatty acid (SCFA) concentration throughout the GIT, nutrient digestibility, GIT barrier function, ruminal SCFA absorption, ruminal morphometrics, intestinal brush border enzyme activity, and blood parameters for beef heifers. Thirty-two beef heifers fitted with ruminal cannulas were used in a randomized complete block design and assigned to one of four treatments: 1) negative control (ruminal infusion of double-distilled water; CON); 2) abomasal infusion of CaB (AB; 0.0029% of BW); 3) abomasal infusion of CaG (AG; 0.0077% of BW); and 4) ruminal infusion of a hydrogenated fat-embedded CaG (RG; 0.0192% of BW) to provide ruminal protection. Excluding CON, treatments were designed to deliver the same amount of butyrate in the small intestine. Heifers were housed in individual pens and DMI was limited to 95% of voluntary intake to minimize a potential confounding effect of DMI on treatment responses. Total GIT barrier function was assessed on day 17 and SCFA disappearance was evaluated on day 21 using the temporarily isolated and washed reticulo-rumen technique. On day 28, heifers were slaughtered, and ruminal and colonic digesta were collected to assess SCFA concentration. Additionally, ruminal, jejunal, and colonic tissues were collected to assess SCFA fluxes and regional barrier function ex vivo using the Ussing chamber technique. For colonic digesta, both AB and CaG treatments reduced the proportion of acetate (P < 0.05) and increased the proportion on propionate (P < 0.05) compared to CON. Relative to CON, AB but not CaG treatments increased in vivo ruminal disappearance of total SCFA (P = 0.01), acetate (P = 0.03), propionate (P = 0.01), and butyrate (P > 0.01). Treatments did not affect (P ≥ 0.10) acetate and butyrate fluxes in the ruminal and colonic tissues when measured ex vivo; however, when compared with CON, AB tended to decrease (P = 0.09) mannitol flux across ruminal tissue. In addition, mannitol flux was affected (P < 0.01) by region, with greater mannitol flux across the jejunum than rumen and colon. We conclude that while both abomasal infusion of CaB and CaG affect the molar proportion of acetate and propionate in the colon, only abomasal CaB stimulated ruminal SCFA absorption for growing beef heifers.
Butyrate supplementation has been proven to enhance gastrointestinal tract development and functionality. This study evaluates the mode of action and the effects of Ca-gluconate relative to butyrate on gastrointestinal tract function in beef heifers.
Lay Summary
Butyrate, a short-chain fatty acid (SCFA), has received attention due to its ability to promote gastrointestinal (GIT) health and development. However, butyrate in its free form presents a strong odor, limiting its use in diet formulation. Supplementation of butyrate precursors, such as gluconate, have been studied to enhance butyrate production in the GIT. This study evaluated the effects of post-ruminal infusion of Ca-butyrate (AB; 0.0029% of BW) and Ca-gluconate (AG; 0.0077% of BW) and ruminal infusion of a hydrogenated fat-embedded Ca-gluconate (RG; 0.0192% of BW) relative to control (CON; ruminal infusion of double-distilled water). Thirty-two beef heifers fitted with ruminal cannulas were fed for 28 d and GIT barrier function and ruminal SCFA absorption were assessed. At slaughter, the rumen, jejunum, and colon tissues were collected and barrier function and SCFA fluxes were assessed ex vivo. Relative to CON, AB but not AG and RG increased in vivo ruminal SCFA absorption and tended to increase ex vivo barrier function. Thus, the data presented in this study shows that butyrate and gluconate do not function through the same mode of action in the GIT of beef heifers.</abstract><cop>US</cop><pub>Oxford University Press</pub><pmid>36799118</pmid><doi>10.1093/jas/skad050</doi><orcidid>https://orcid.org/0000-0001-6208-8720</orcidid><orcidid>https://orcid.org/0000-0002-6396-2130</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-8812 |
| ispartof | Journal of animal science, 2023-01, Vol.101 |
| issn | 0021-8812 1525-3163 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2788800646 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Absorption Acetic acid Animal Feed - analysis Animals Beef Butyrates - metabolism Butyrates - pharmacology Cattle Colon Diet - veterinary Digestibility Digestion - physiology Distilled water Dosage Enzymatic activity Enzyme activity Enzymes Fatty acids Fatty Acids, Volatile - metabolism Female Fermentation Fluctuations Fluxes Gluconates - metabolism Histology Intestinal Absorption Intestine Jejunum Mannitol Microvilli Morphometry Nutrient concentrations Propionates - metabolism Propionic acid Rumen Rumen - metabolism Small intestine Trinucleotide repeats |
| title | A comparison of post-ruminal provision of Ca-gluconate and Ca-butyrate on growth performance, gastrointestinal barrier function, short-chain fatty acid absorption, intestinal histology, and brush-border enzyme activity in beef heifers |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T20%3A03%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20comparison%20of%20post-ruminal%20provision%20of%20Ca-gluconate%20and%20Ca-butyrate%20on%20growth%20performance,%20gastrointestinal%20barrier%20function,%20short-chain%20fatty%20acid%20absorption,%20intestinal%20histology,%20and%20brush-border%20enzyme%20activity%20in%20beef%20heifers&rft.jtitle=Journal%20of%20animal%20science&rft.au=Watanabe,%20Daniel%20H%20M&rft.date=2023-01-03&rft.volume=101&rft.issn=0021-8812&rft.eissn=1525-3163&rft_id=info:doi/10.1093/jas/skad050&rft_dat=%3Cproquest_cross%3E2788800646%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3041053306&rft_id=info:pmid/36799118&rft_oup_id=10.1093/jas/skad050&rfr_iscdi=true |