Decorin inhibits the formation of hard nodules after microwave ablation by inhibiting the TGF-β1/SMAD and MAPK signaling pathways: in a Bama miniature pig model of mammary gland hyperplasia
Benign breast lesions are often associated with hard nodule formation after microwave ablation (MWA), which persists for a long time and causes problems in patients. The aim of this study was to evaluate the efficacy of decorin in the treatment of hard nodule formation and its potential mechanism of...
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| Veröffentlicht in: | International journal of hyperthermia 2023-12, Vol.40 (1), p.2188151-2188151 |
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| container_title | International journal of hyperthermia |
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| creator | Du, Yue Liu, Xinyao Du, Kai Zhang, Wenkai Li, Rui Yang, Lizhi Cheng, Linggang He, Wen Zhang, Wei |
| description | Benign breast lesions are often associated with hard nodule formation after microwave ablation (MWA), which persists for a long time and causes problems in patients. The aim of this study was to evaluate the efficacy of decorin in the treatment of hard nodule formation and its potential mechanism of action.
Using a Bama miniature pig model of mammary gland hyperplasia, immunohistochemistry, Masson's trichrome and western blotting were firstly applied to compare the extent of fibrosis and activation of key members of the TGF-β1/SMAD and MAPK signaling pathways of hard nodule in the control and MWA groups, and then the extent of fibrosis and expression of signaling pathways in hard nodule were examined after application of decorin.
The results showed that the MWA group had increased levels of TGF-β1, p-SMAD2/3, p-ERK1/2, and collagen I proteins and increased fibrosis at 2 weeks, 4 weeks, and 3 months after MWA. After decorin treatment, the expression levels of each protein were significantly downregulated, and the degree of fibrosis was reduced at 2 weeks, 4 weeks, and 3 months after MWA compared with the MWA group.
In conclusion, these results suggest that activation of TGF-β1 may play an important role in hard nodule formation and that decorin may reduce hard nodule formation after MWA in a model of mammary gland hyperplasia by inhibiting the TGF-β1/SMAD and MAPK signaling pathways. |
| doi_str_mv | 10.1080/02656736.2023.2188151 |
| format | Article |
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Using a Bama miniature pig model of mammary gland hyperplasia, immunohistochemistry, Masson's trichrome and western blotting were firstly applied to compare the extent of fibrosis and activation of key members of the TGF-β1/SMAD and MAPK signaling pathways of hard nodule in the control and MWA groups, and then the extent of fibrosis and expression of signaling pathways in hard nodule were examined after application of decorin.
The results showed that the MWA group had increased levels of TGF-β1, p-SMAD2/3, p-ERK1/2, and collagen I proteins and increased fibrosis at 2 weeks, 4 weeks, and 3 months after MWA. After decorin treatment, the expression levels of each protein were significantly downregulated, and the degree of fibrosis was reduced at 2 weeks, 4 weeks, and 3 months after MWA compared with the MWA group.
In conclusion, these results suggest that activation of TGF-β1 may play an important role in hard nodule formation and that decorin may reduce hard nodule formation after MWA in a model of mammary gland hyperplasia by inhibiting the TGF-β1/SMAD and MAPK signaling pathways.</description><identifier>ISSN: 0265-6736</identifier><identifier>EISSN: 1464-5157</identifier><identifier>DOI: 10.1080/02656736.2023.2188151</identifier><identifier>PMID: 36919520</identifier><language>eng</language><publisher>England: Taylor & Francis</publisher><subject>Animals ; benign breast lesions ; decorin ; Decorin - metabolism ; Decorin - pharmacology ; Fibrosis ; hard nodule ; Hyperplasia ; MAP Kinase Signaling System ; Microwave ablation ; Microwaves ; Signal Transduction ; Swine ; Swine, Miniature - metabolism ; TGF-β1 ; Transforming Growth Factor beta1 - metabolism</subject><ispartof>International journal of hyperthermia, 2023-12, Vol.40 (1), p.2188151-2188151</ispartof><rights>2023 The Author(s). Published with license by Taylor & Francis Group, LLC 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-99761b3c8c816c9ec7ad6cb9a8376893ccfa2e9b3f0804547f20a1a480cde3aa3</citedby><cites>FETCH-LOGICAL-c479t-99761b3c8c816c9ec7ad6cb9a8376893ccfa2e9b3f0804547f20a1a480cde3aa3</cites><orcidid>0000-0003-2389-2366</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/02656736.2023.2188151$$EPDF$$P50$$Ginformaworld$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/02656736.2023.2188151$$EHTML$$P50$$Ginformaworld$$Hfree_for_read</linktohtml><link.rule.ids>315,781,785,865,2103,27507,27929,27930,59148,59149</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36919520$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Du, Yue</creatorcontrib><creatorcontrib>Liu, Xinyao</creatorcontrib><creatorcontrib>Du, Kai</creatorcontrib><creatorcontrib>Zhang, Wenkai</creatorcontrib><creatorcontrib>Li, Rui</creatorcontrib><creatorcontrib>Yang, Lizhi</creatorcontrib><creatorcontrib>Cheng, Linggang</creatorcontrib><creatorcontrib>He, Wen</creatorcontrib><creatorcontrib>Zhang, Wei</creatorcontrib><title>Decorin inhibits the formation of hard nodules after microwave ablation by inhibiting the TGF-β1/SMAD and MAPK signaling pathways: in a Bama miniature pig model of mammary gland hyperplasia</title><title>International journal of hyperthermia</title><addtitle>Int J Hyperthermia</addtitle><description>Benign breast lesions are often associated with hard nodule formation after microwave ablation (MWA), which persists for a long time and causes problems in patients. The aim of this study was to evaluate the efficacy of decorin in the treatment of hard nodule formation and its potential mechanism of action.
Using a Bama miniature pig model of mammary gland hyperplasia, immunohistochemistry, Masson's trichrome and western blotting were firstly applied to compare the extent of fibrosis and activation of key members of the TGF-β1/SMAD and MAPK signaling pathways of hard nodule in the control and MWA groups, and then the extent of fibrosis and expression of signaling pathways in hard nodule were examined after application of decorin.
The results showed that the MWA group had increased levels of TGF-β1, p-SMAD2/3, p-ERK1/2, and collagen I proteins and increased fibrosis at 2 weeks, 4 weeks, and 3 months after MWA. After decorin treatment, the expression levels of each protein were significantly downregulated, and the degree of fibrosis was reduced at 2 weeks, 4 weeks, and 3 months after MWA compared with the MWA group.
In conclusion, these results suggest that activation of TGF-β1 may play an important role in hard nodule formation and that decorin may reduce hard nodule formation after MWA in a model of mammary gland hyperplasia by inhibiting the TGF-β1/SMAD and MAPK signaling pathways.</description><subject>Animals</subject><subject>benign breast lesions</subject><subject>decorin</subject><subject>Decorin - metabolism</subject><subject>Decorin - pharmacology</subject><subject>Fibrosis</subject><subject>hard nodule</subject><subject>Hyperplasia</subject><subject>MAP Kinase Signaling System</subject><subject>Microwave ablation</subject><subject>Microwaves</subject><subject>Signal Transduction</subject><subject>Swine</subject><subject>Swine, Miniature - metabolism</subject><subject>TGF-β1</subject><subject>Transforming Growth Factor beta1 - metabolism</subject><issn>0265-6736</issn><issn>1464-5157</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>EIF</sourceid><sourceid>DOA</sourceid><recordid>eNp9kstu1DAUhiMEokPhEUBessnUl8SxWTG0tFS0Aomyto4vmXGVxKmdYTSv1QfoI_BMJHPpko0tWd__n-Nz_ix7T_CcYIHPMOUlrxifU0zZnBIhSEleZDNS8CIvSVm9zGYTk0_QSfYmpXuMcVHS6nV2wrgksqR4lj1dOBOi75DvVl77IaFh5VAdYguDDx0KNVpBtKgLdt24hKAeXEStNzFs4I9DoJs9qLdHC98tdyZ3V5f530dy9ut2cYGgs-h28fM7Sn7ZQTMxPQyrDWzTp1GIAH2BFkbjzsOwjg71fonaYF0ztdBC20LcomUz-ay2vYt9A8nD2-xVDU1y7w73afb78uvd-bf85sfV9fniJjdFJYdcyooTzYwwgnAjnanAcqMlCFZxIZkxNVAnNavH0RZlUdUUA4FCYGMdA2Cn2fXe1wa4V330UzsqgFe7hxCXCuLgTeOUxpwYKyjWRhSaW4GZLAotHUhWlByPXh_3Xn0MD2uXBtX6ZFwz_s2FdVK0EhUlZDxGtNyj47hTiq5-Lk2wmmKgjjFQUwzUIQaj7sOhxFq3zj6rjnsfgc97wHe7XW9CbKwaYNuEWEfojE-K_b_GP174xBE</recordid><startdate>20231231</startdate><enddate>20231231</enddate><creator>Du, Yue</creator><creator>Liu, Xinyao</creator><creator>Du, Kai</creator><creator>Zhang, Wenkai</creator><creator>Li, Rui</creator><creator>Yang, Lizhi</creator><creator>Cheng, Linggang</creator><creator>He, Wen</creator><creator>Zhang, Wei</creator><general>Taylor & Francis</general><general>Taylor & Francis Group</general><scope>0YH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-2389-2366</orcidid></search><sort><creationdate>20231231</creationdate><title>Decorin inhibits the formation of hard nodules after microwave ablation by inhibiting the TGF-β1/SMAD and MAPK signaling pathways: in a Bama miniature pig model of mammary gland hyperplasia</title><author>Du, Yue ; Liu, Xinyao ; Du, Kai ; Zhang, Wenkai ; Li, Rui ; Yang, Lizhi ; Cheng, Linggang ; He, Wen ; Zhang, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-99761b3c8c816c9ec7ad6cb9a8376893ccfa2e9b3f0804547f20a1a480cde3aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>benign breast lesions</topic><topic>decorin</topic><topic>Decorin - metabolism</topic><topic>Decorin - pharmacology</topic><topic>Fibrosis</topic><topic>hard nodule</topic><topic>Hyperplasia</topic><topic>MAP Kinase Signaling System</topic><topic>Microwave ablation</topic><topic>Microwaves</topic><topic>Signal Transduction</topic><topic>Swine</topic><topic>Swine, Miniature - metabolism</topic><topic>TGF-β1</topic><topic>Transforming Growth Factor beta1 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Du, Yue</creatorcontrib><creatorcontrib>Liu, Xinyao</creatorcontrib><creatorcontrib>Du, Kai</creatorcontrib><creatorcontrib>Zhang, Wenkai</creatorcontrib><creatorcontrib>Li, Rui</creatorcontrib><creatorcontrib>Yang, Lizhi</creatorcontrib><creatorcontrib>Cheng, Linggang</creatorcontrib><creatorcontrib>He, Wen</creatorcontrib><creatorcontrib>Zhang, Wei</creatorcontrib><collection>Access via Taylor & Francis (Open Access Collection)</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>International journal of hyperthermia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Du, Yue</au><au>Liu, Xinyao</au><au>Du, Kai</au><au>Zhang, Wenkai</au><au>Li, Rui</au><au>Yang, Lizhi</au><au>Cheng, Linggang</au><au>He, Wen</au><au>Zhang, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decorin inhibits the formation of hard nodules after microwave ablation by inhibiting the TGF-β1/SMAD and MAPK signaling pathways: in a Bama miniature pig model of mammary gland hyperplasia</atitle><jtitle>International journal of hyperthermia</jtitle><addtitle>Int J Hyperthermia</addtitle><date>2023-12-31</date><risdate>2023</risdate><volume>40</volume><issue>1</issue><spage>2188151</spage><epage>2188151</epage><pages>2188151-2188151</pages><issn>0265-6736</issn><eissn>1464-5157</eissn><abstract>Benign breast lesions are often associated with hard nodule formation after microwave ablation (MWA), which persists for a long time and causes problems in patients. The aim of this study was to evaluate the efficacy of decorin in the treatment of hard nodule formation and its potential mechanism of action.
Using a Bama miniature pig model of mammary gland hyperplasia, immunohistochemistry, Masson's trichrome and western blotting were firstly applied to compare the extent of fibrosis and activation of key members of the TGF-β1/SMAD and MAPK signaling pathways of hard nodule in the control and MWA groups, and then the extent of fibrosis and expression of signaling pathways in hard nodule were examined after application of decorin.
The results showed that the MWA group had increased levels of TGF-β1, p-SMAD2/3, p-ERK1/2, and collagen I proteins and increased fibrosis at 2 weeks, 4 weeks, and 3 months after MWA. After decorin treatment, the expression levels of each protein were significantly downregulated, and the degree of fibrosis was reduced at 2 weeks, 4 weeks, and 3 months after MWA compared with the MWA group.
In conclusion, these results suggest that activation of TGF-β1 may play an important role in hard nodule formation and that decorin may reduce hard nodule formation after MWA in a model of mammary gland hyperplasia by inhibiting the TGF-β1/SMAD and MAPK signaling pathways.</abstract><cop>England</cop><pub>Taylor & Francis</pub><pmid>36919520</pmid><doi>10.1080/02656736.2023.2188151</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-2389-2366</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals benign breast lesions decorin Decorin - metabolism Decorin - pharmacology Fibrosis hard nodule Hyperplasia MAP Kinase Signaling System Microwave ablation Microwaves Signal Transduction Swine Swine, Miniature - metabolism TGF-β1 Transforming Growth Factor beta1 - metabolism |
| title | Decorin inhibits the formation of hard nodules after microwave ablation by inhibiting the TGF-β1/SMAD and MAPK signaling pathways: in a Bama miniature pig model of mammary gland hyperplasia |
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